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The Anatomical Record Feb 1999Deciduoma induced by mechanical stimulation in pseudopregnant mice is similar to the decidua in normal pregnancy and it undergoes regression after a certain period.... (Comparative Study)
Comparative Study
Deciduoma induced by mechanical stimulation in pseudopregnant mice is similar to the decidua in normal pregnancy and it undergoes regression after a certain period. Therefore, we examined cell death in deciduomas which were induced by artificial stimulation. To analyze the regression mechanism of artificially induced deciduoma, DNA fragmentation, in situ 3'-DNA nick end labeling, and RT-PCR were performed on day 6 to 14 of pseudopregnancy. DNA fragmentation appeared on day 8 and it increased to day 10 of pseudopregnancy in the traumatized uterine horn. A large number of apoptotic cells were found on day 10 in the periphery of deciduoma at the antimesometrial side. Deciduoma underwent degeneration on day 11 of pseudopregnancy. Expression of tumor necrosis factor-alpha (TNF-alpha) mRNA was high on days 8 and 10, then decreased, whereas the expression increased again on day 14. TNF-alpha protein was expressed from day 8 to day 12, showing a peak expression on day 10 when deciduoma reached maximum weight. Serum progesterone level was high in the traumatized pseudopregnant mice on day 6, then it gradually decreased. Life span of deciduoma was prolonged 4 days more by daily injection of progesterone. A reduction in serum progesterone coincides with TNF-alpha increase, resulting in an increase of apoptotic deciduomal cells at the regression period, and that the life span of deciduoma is prolonged by additive supply of progesterone.
Topics: Animals; Apoptosis; DNA Fragmentation; Decidua; Electrophoresis, Agar Gel; Female; Immunoblotting; In Situ Hybridization; Mice; Mice, Inbred ICR; Progesterone; Pseudopregnancy; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Tumor Necrosis Factor-alpha; Uterus
PubMed: 9972805
DOI: 10.1002/(SICI)1097-0185(19990201)254:2<205::AID-AR6>3.0.CO;2-K -
Biology of Reproduction May 1979
Topics: Animals; Chemical Fractionation; Decidua; Electrophoresis, Polyacrylamide Gel; Endometrium; Female; Leucine; Molecular Weight; Myometrium; Organ Size; Pregnancy; Protein Biosynthesis; Proteins; Rats; Uterine Diseases; Uterus
PubMed: 454767
DOI: 10.1095/biolreprod20.4.811 -
Nature Sep 1959
Topics: Animals; Carbonic Anhydrases; Decidua; Deciduoma; Female; Humans; Rats
PubMed: 14407524
DOI: 10.1038/184961a0 -
Acta Anatomica 1994Decidual cell induction in the pseudopregnant rat was examined in this study using the lectin concanavalin A (ConA). The histochemical binding of the lectin to the...
Decidual cell induction in the pseudopregnant rat was examined in this study using the lectin concanavalin A (ConA). The histochemical binding of the lectin to the uterine cell surface at the time of deciduomatic induction was also studied. ConA was found to induce significant deciduomata (decidual-like tissue) in the uterine horn when injected intraluminally on day 5 of pseudopregnancy (PSP). ConA-induced deciduomata appeared as a series of discrete nodules in the uterine horn, reminiscent of the anatomical appearance of normal embryo implantation sites. Deciduoma induction by ConA was greatly reduced by pre-absorption of the lectin with its competitive sugar. Lectin histochemistry revealed binding of ConA to the cell surface on day 5 of PSP. Pre-absorption of the lectin with its competitive sugar also significantly reduced surface binding of the lectin, and this finding may be correlated with the greatly reduced ability of the pre-absorbed lectin to induce deciduomata. Possible mechanisms for the induction of deciduomata by lectins are considered.
Topics: Animals; Concanavalin A; Deciduoma; Female; Organ Size; Pregnancy; Pseudopregnancy; Rats; Uterus
PubMed: 14758848
DOI: 10.1159/000147659 -
Nature Oct 1964
Topics: Animals; Biological Assay; Chorionic Gonadotropin; Decidua; Deciduoma; Female; Gonadotropins; Gonadotropins, Pituitary; Humans; Mice; Pituitary Gland; Research; Wounds and Injuries
PubMed: 14228880
DOI: 10.1038/204385a0 -
The Indian Medical Gazette Jan 1897
Obstetrics and Gynæcology: Deciduoma Malignum-Ovarian Dermoids-Methyl Blue in Inoperable Cancer-Ovarian Therapy-Contribution to the Study of Ovulation, Menstruation and Conception-Position of the Normal Ovary-Physiology of Menstruation.
PubMed: 29002763
DOI: No ID Found -
Obstetricia Y Ginecologia... 1957
Topics: Deciduoma; Female; Humans; Neoplasms; Placenta; Polyps; Pregnancy
PubMed: 13452301
DOI: No ID Found -
Annals of Surgery May 1895
PubMed: 17860165
DOI: 10.1097/00000658-189521060-00050 -
Gynecologic and Obstetric Investigation 1983The relationships between experimentally induced deciduoma formation, circulating estradiol (E) and progesterone (P) levels and alterations in uterine blood flow (UBF)...
The relationships between experimentally induced deciduoma formation, circulating estradiol (E) and progesterone (P) levels and alterations in uterine blood flow (UBF) were studied between days 4 and 15 of pseudopregnancy (PSP: day 0 = ovulation) in rats. Blood flow was measured with an electromagnetic blood flow monitor and serum analyzed for E and P levels by radioimmunoassay. Neither uterine trauma on day 4 of PSP nor the site of trauma had any direct influence on altering UBF. A dramatic increase in UBF occurred in response to stromal proliferation on days 5-6 and continued to remain above sham-operated control levels through day 9. These vascular changes correlated temporally with the day 9 peak in uterine weight resulting from deciduoma formation. Both uterine weight and vascular fluctuations in deciduoma-bearing rats were related to the cyclic changes in E/P ratios between days 5 and 8 of PSP. A decline in UBF preceded deciduoma regression between days 9 and 15 of PSP. These results suggest that an increase in UBF is causally associated with the formation and maintenance of deciduoma in the PSP rat and that deciduoma regression may result from a subsequent decline in UBF rates. Both factors may be directly dependent on fluctuating E/P ratios.
Topics: Animals; Blood Flow Velocity; Decidua; Estradiol; Female; Organ Size; Pregnancy; Progesterone; Pseudopregnancy; Rats; Rats, Inbred Strains; Regional Blood Flow; Uterus
PubMed: 6826114
DOI: 10.1159/000299398 -
Endocrinology Feb 1981Nuclear estrogen receptor levels were measured in fractions of dispersed deciduoma cells separated according to size by velocity sedimentation at unit gravity. Previous...
Nuclear estrogen receptor levels were measured in fractions of dispersed deciduoma cells separated according to size by velocity sedimentation at unit gravity. Previous studies demonstrated that velocity sedimentation separated larger fully differentiated polyploid deciduoma cells from smaller diploid cells. Since levels of estrogen receptor measured in homogenates of deciduoma tissue decreased during the growth and differentiation of the deciduoma, lower levels of estrogen receptor per cell were anticipated in polyploid deciduoma cells. Although levels of estrogen receptor decreased slightly in all fractions of deciduoma cells from days 7-8 of pseudopregnancy, the larger polyploid cells had higher levels of estrogen receptor than smaller cells on both days. These results indicate that differentiation of endometrial stromal cells during decidualization involves a decrease in receptor levels in all deciduomal cell types rather than a specific decrease in the receptor levels of only fully differentiated deciduoma cells. (Endocrinology 108: 484, 1981)
Topics: Animals; Cell Nucleus; Cell Separation; Centrifugation; DNA; Decidua; Estradiol; Female; In Vitro Techniques; Ploidies; Pseudopregnancy; Rats; Receptors, Estrogen
PubMed: 7449737
DOI: 10.1210/endo-108-2-484