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Ars Curandi Em Odontologia Oct 1980
Topics: Biochemical Phenomena; Biochemistry; Dental Pulp; Humans
PubMed: 6938179
DOI: No ID Found -
Zhonghua Kou Qiang Yi Xue Za Zhi =... Jan 2018To evaluate the effect of vascular endothelial growth factor (VEGF)-loaded microspheres on dental pulp tissue regeneration and vascularization . release experiment and...
To evaluate the effect of vascular endothelial growth factor (VEGF)-loaded microspheres on dental pulp tissue regeneration and vascularization . release experiment and human umbilical vein endothelial cell migration experiment were conducted with VEGF loaded microspheres. The dental pulp stem cells (DPSC) were co-cultured with VEGF microspheres to observe the compatibility between the cells and the microspheres. DPSC and VEGF loaded microspheres were injected into the root lumen through the apical foramen, which were then transplanted subcutaneously into nude mice. Histological and immunohistochemical features were observed after nine weeks. DPSCs attached and spread on the surface of the microspheres. HE staining showed that the regenerated pulp-like tissue fulfilled the whole apex and middle third of the root. Differentiated odontoblast-like cells aligned with the existing tubular root dentin. VEGF-loaded microspheres promoted the regeneration of pulp-like tissues and formation of blood vessels.
Topics: Animals; Cell Differentiation; Cell Movement; Coculture Techniques; Dental Pulp; Human Umbilical Vein Endothelial Cells; Humans; Mice; Mice, Nude; Microspheres; Neovascularization, Physiologic; Odontoblasts; Regeneration; Stem Cell Transplantation; Stem Cells; Vascular Endothelial Growth Factor A
PubMed: 29972963
DOI: 10.3760/cma.j.issn.1002-0098.2018.01.009 -
Acta Odontologica Scandinavica Aug 1983This article deals with blood flow and tissue pressure changes in the inflamed dental pulp. While detailed patho-physiological studies of the circulation in the inflamed... (Comparative Study)
Comparative Study
This article deals with blood flow and tissue pressure changes in the inflamed dental pulp. While detailed patho-physiological studies of the circulation in the inflamed pulp are still partly lacking, available data from studies of the normal pulp tissue are also discussed. Knowledge of the normal physiological adjustments may indicate which circulatory changes would take place during inflammation. One of the focal points in the present article is how a localized increased tissue pressure may persist in the inflamed area without a circumferential spread to the rest of the pulp. The article is organized in the following manner: First a short review is given of the vascular changes during inflammation in general. Then available data from morphological and functional studies of the pulpal circulation are treated in some detail. Particular attention is given to the transcapillary fluid flow and the so-called 'self-strangulation theory'. The effect of a localized increased tissue pressure on pulpal blood flow is thereafter discussed.
Topics: Animals; Capillary Permeability; Dental Pulp; Dilatation, Pathologic; Humans; Pressure; Pulpitis; Vasodilation
PubMed: 6578666
DOI: 10.3109/00016358309162331 -
Oral Diseases Jan 2015We previously reported that matrix metalloproteinase-3(MMP-3) accelerates wound healing following dental pulp injury. In this study, we tested the hypothesis that...
OBJECTIVE
We previously reported that matrix metalloproteinase-3(MMP-3) accelerates wound healing following dental pulp injury. In this study, we tested the hypothesis that induction of MMP-3 activity by interleukin-1β would promote proliferation and apoptosis of dental pulp cells.
MATERIALS AND METHODS
Dental pulp cells were isolated from rat incisors and subjected to interleukin-1β. Matrix metalloproteinase-3 mRNA and protein expression were assessed using reverse transcription-polymerase chain reaction and Western blotting, respectively. Matrix metalloproteinase-3 activity was measured using fluorescence. Dental pulp cell proliferation and apoptosis were determined using enzyme-linked immunosorbent assays (ELISA) for BrdU and DNA fragmentation, respectively. siRNA was used to reduce MMP-3 transcripts in these cells.
RESULTS
Treatment with interleukin-1β increased MMP-3 mRNA and protein levels as well as its activity in dental pulp cells. Cell proliferation was also markedly increased, with no changes in apoptosis observed. Treatment with siRNA against MMP-3 potently suppressed this interleukin-1β-induced increase in MMP-3 expression and activity, and also suppressed cell proliferation but unexpectedly increased apoptosis in these cells (P < 0.05). This siRNA-mediated increase in apoptosis could be reversed with exogenous MMP-3 stimulation (P < 0.05).
CONCLUSIONS
Interleukin-1β induces MMP-3-regulated cell proliferation and suppresses apoptosis in dental pulp cells.
Topics: Animals; Apoptosis; Blotting, Western; Cell Proliferation; Dental Pulp; Interleukin-1beta; Matrix Metalloproteinase 3; Rats; Reverse Transcriptase Polymerase Chain Reaction
PubMed: 24330147
DOI: 10.1111/odi.12219 -
Proceedings of the Finnish Dental... 1986
Review
Topics: Animals; Dental Pulp; Dental Pulp Diseases; Humans; Toothache
PubMed: 3543931
DOI: No ID Found -
Australian Dental Journal Aug 1992
Topics: Anesthesia, Dental; Anesthetics; Dental Pulp; Humans; Syndrome; Toothache
PubMed: 1444952
DOI: No ID Found -
The Journal of Physiology May 2006
Review
Topics: Dental Pulp; Humans; Models, Biological; Pulpitis
PubMed: 16581853
DOI: 10.1113/jphysiol.2006.110049 -
Romanian Journal of Morphology and... 2011The diagnosis of occlusion-generated disorders of the dento-maxillary apparatus represents a sensitive stage within the establishment of the therapeutic means for the...
The diagnosis of occlusion-generated disorders of the dento-maxillary apparatus represents a sensitive stage within the establishment of the therapeutic means for the functional rehabilitation of dental arches. The laborious effort carried out in order to specify the diagnosis resides in the fact that any trauma arising at the level of any component of the stomatognate system may lead to an occlusal dysfunction. The uncured carious processes, besides the pulp and periapical complications, may lead to an occlusal dysfunction through horizontal migrations of teeth resulting in the derangement of the occlusal curvatures as well as through vertical migrations of the teeth opposing a tooth diagnosed with occlusal caries or which largely reduced the coronary height. The dental iatrogenia, besides the eructation anomalies and neuromuscular dysfunction within the oromaxillofacial area also determines the appearance of occlusal dysfunction. The radiological examination through correlation with the clinical manifestations may provide significant data related to the magnitude of the prejudice caused to dento-paradontal units experiencing occlusal trauma. The histopathological study through correlation with the clinical manifestations provides significant data on the tolerance of dento-paradontal units within the occlusal dysfunction. Also, subsequent to the analysis of the possible actions of aggression generated by the occlusal trauma correlations could be determined between the type of the histological lesion of the pulp-dentine complex and the etiopathogenic factors, as well as correlations depending on the damage degree through occlusal trauma of the dental parts involved.
Topics: Atrophy; Calcinosis; Dental Occlusion; Dental Pulp; Humans; Radiography; Wounds and Injuries
PubMed: 21424087
DOI: No ID Found -
Journal of Oral Pathology Feb 1985Four adult Rhesus monkeys provided 120 teeth for buccal Class V cavities. Twenty-nine were non-exposed controls and 91 were exposed for 3 intervals. All 120 teeth were...
Four adult Rhesus monkeys provided 120 teeth for buccal Class V cavities. Twenty-nine were non-exposed controls and 91 were exposed for 3 intervals. All 120 teeth were capped with a hard set Ca(OH)2 medicament, restored with amalgam, 57 evaluated after 1 year and 63 after 2 years. Of the 91 exposed pulps, 45 showed complete healing, 25 showed pulpal inflammation varying from acute to chronic, 12 showed severe pulpal breakdown and abscess formation and 9 were necrotic. No difference was observed in the healing response between the 3 exposure times. New hard tissue formed at, or subjacent to, the medicament in 77 of 91 exposed pulps with a tunnel defect frequently present, running from the medicament interface to the pulp. This study demonstrates that recurring pulp inflammation observed after 1 & 2 year direct pulp capping, is associated with bacterial contamination.
Topics: Animals; Calcium Hydroxide; Dental Amalgam; Dental Pulp; Dental Pulp Capping; Dental Pulp Exposure; Macaca mulatta; Pulpitis; Time Factors; Wound Healing
PubMed: 3920366
DOI: 10.1111/j.1600-0714.1985.tb00479.x -
The Journal of Prosthetic Dentistry Sep 1982The stressed pulp condition is a clinical concept that describes pulps that have received repeated previous injury and survived with diminished responses and lessened... (Review)
Review
The stressed pulp condition is a clinical concept that describes pulps that have received repeated previous injury and survived with diminished responses and lessened repair potentials. Before performing restorative dentistry the dentist should conduct a comprehensive pulpal health evaluation on teeth to be restored. This evaluation should include (1) traditional pulp-testing methods and (2) a review of the past, present, and planned future treatment of the tooth. This analysis will usually identify teeth with stressed pulp conditions. Teeth with stressed pulps should be treated before complex restorative dentistry.
Topics: Crowns; Dental Pulp; Dental Pulp Diseases; Dental Pulp Test; Humans; Root Canal Therapy; Stress, Mechanical
PubMed: 6750089
DOI: 10.1016/0022-3913(82)90008-7