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Journal of Biochemical and Biophysical... Sep 2005Evaluation of glucose uptake ability in cells plays a fundamental role in diabetes mellitus research. In this study, we describe a sensitive and non-radioactive assay...
Evaluation of glucose uptake ability in cells plays a fundamental role in diabetes mellitus research. In this study, we describe a sensitive and non-radioactive assay for direct and rapid measuring glucose uptake in single, living cells. The assay is based on direct incubation of mammalian cells with a fluorescent d-glucose analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (2-NBDG) followed by flow cytometric detection of fluorescence produced by the cells. A series of experiments were conducted to define optimal conditions for this assay. By this technique, it was found that insulin lost its physiological effects on cells in vitro meanwhile some other anti-diabetic drugs facilitated the cell glucose uptake rates with mechanisms which likely to be different from those of insulin or those that were generally accepted of each drug. Our findings show that this technology has potential for applications in both medicine and research.
Topics: 4-Chloro-7-nitrobenzofurazan; Biological Transport; Cell Line, Tumor; Deoxyglucose; Flow Cytometry; Fluorescence; Fluorescent Dyes; Glucose; Humans
PubMed: 16182371
DOI: 10.1016/j.jbbm.2005.08.001 -
Biochemical Pharmacology Jun 1987
Review
Topics: Animals; Brain; Deoxy Sugars; Deoxyglucose; Energy Metabolism; Fluorodeoxyglucose F18; Glucose; Humans; Mathematics
PubMed: 3297067
DOI: 10.1016/0006-2952(87)90480-1 -
Cancer Biotherapy & Radiopharmaceuticals Jun 2008There are several radionuclide-labeled derivatives of deoxyglucose (DG) that have been developed including 2-fluro-deoxyglucose, ethylenedicysteine-deoxyglucose,... (Review)
Review
There are several radionuclide-labeled derivatives of deoxyglucose (DG) that have been developed including 2-fluro-deoxyglucose, ethylenedicysteine-deoxyglucose, diethylenetriaminepentaacetate-deoxyglucose, N-(2'-hydroxybenzyl)-2-amino-2-deoxy-D-glucose, and methyl D-glucoside that were synthesized and successfully labeled in high labeling fields. The former 4 were used for tumor imaging and methyl-D-glucoside for the diagnosis and the monitoring of the functional status of renal tubules. These derivatives are suitable for imaging examinations when labeled with either fluorine-18 (18F), technetium-99m (99mTc), carbon-11 (11C), or gallium-68 (68Ga). These compounds are suitable both for imaging and for therapy if labeled with rhenium-188 (188Re). In the area of molecular imaging of nuclear medicine, derivatives of radionuclide-labeled deoxyglucose will become an important tool for the diagnosis and carcinoma treatment in the clinic.
Topics: Animals; Deoxyglucose; Diagnostic Imaging; Fluorine Radioisotopes; Fluorodeoxyglucose F18; Humans; Mice; Neoplasm Transplantation; Neoplasms; Pentetic Acid; Positron-Emission Tomography; Radioisotopes; Rhenium; Technetium; Tomography, Emission-Computed, Single-Photon
PubMed: 18593371
DOI: 10.1089/cbr.2007.0443 -
Biochemical Pharmacology Nov 2014The use of energy restriction mimetic agents (ERMAs) to selectively target cancer cells addicted to glycolysis could be a promising therapeutic approach.... (Review)
Review
The use of energy restriction mimetic agents (ERMAs) to selectively target cancer cells addicted to glycolysis could be a promising therapeutic approach. Thiazolidinediones (TZDs) are synthetic agonists of the nuclear receptor peroxisome proliferator-activated receptor (PPAR)γ that were developed to treat type II diabetes. These compounds also display anticancer effects which appear mainly to be independent of their PPARγ agonist activity but the molecular mechanisms involved in the anticancer action are not yet well understood. Results obtained on ciglitazone derivatives, mainly in prostate cancer cell models, suggest that these compounds could act as ERMAs. In the present paper, we introduce how compounds like 2-deoxyglucose target the Warburg effect and then we discuss the possibility that the PPARγ-independent effects of various TZD could result from their action as ERMAs.
Topics: Antineoplastic Agents; Apoptosis; Autophagy; Cell Line, Tumor; Deoxyglucose; Energy Metabolism; Humans; Neoplasms; Thiazolidinediones
PubMed: 25083915
DOI: 10.1016/j.bcp.2014.07.021 -
Nuclear Medicine and Biology Oct 1995
Review
Topics: Animals; Deoxyglucose; Fluorine Radioisotopes; Fluorodeoxyglucose F18; Glucose; Hexokinase; Iodine Radioisotopes; Isotope Labeling; Monosaccharide Transport Proteins; Tomography, Emission-Computed, Single-Photon
PubMed: 8547880
DOI: 10.1016/0969-8051(95)00034-u -
Seminars in Nuclear Medicine Oct 1996The use of positron-emission tomography in clinical practice is increasing, particularly with the use of 18-fluoro-2-deoxyglucose (FDG) for oncological studies. As in... (Review)
Review
The use of positron-emission tomography in clinical practice is increasing, particularly with the use of 18-fluoro-2-deoxyglucose (FDG) for oncological studies. As in other imaging modalities, it is important to be aware of normal variants and benign diseases that may mimic more serious pathology. Uptake of FDG in a number of sites may be variable. Uptake of FDG may be seen normally in the skeletal muscle after exercise or under tension, in the myocardium, in parts of the gastrointestinal tract, especially the stomach and cecum, and in the urinary tract. Some causes of increased physiological uptake are avoidable, and measures can be taken to minimize accumulation, thus aiding study interpretation. Inflammatory lesions may cause an increase in FDG uptake, but not usually to the same degree as malignancy. Benign disease such as Paget's disease of bone, sarcoidosis, and tuberculosis may cause uptake that occasionally mimics that of malignancy. Typical examples of a number of physiological and benign variants are described and illustrated.
Topics: Artifacts; Deoxyglucose; Fluorine Radioisotopes; Fluorodeoxyglucose F18; Humans; Tomography, Emission-Computed
PubMed: 8916319
DOI: 10.1016/s0001-2998(96)80006-7 -
Cardiology 1991
Review
Topics: Blood Glucose; Contraindications; Coronary Disease; Deoxyglucose; Energy Metabolism; Fluorodeoxyglucose F18; Humans; Myocardium; Tissue Survival; Tomography, Emission-Computed
PubMed: 1769037
DOI: 10.1159/000174881 -
Journal of Nuclear Medicine : Official... Jul 1992
Topics: Animals; Coronary Circulation; Coronary Disease; Deoxyglucose; Dogs; Fluorodeoxyglucose F18; Humans; Myocardium; Radionuclide Imaging
PubMed: 1613577
DOI: No ID Found -
Progress in Neuro-psychopharmacology &... Sep 19951. To define the neural circuits activated by dopaminergic stimulation in rat models of parkinsonism, the author studied the effects of L-dopa and selective D1 and D2... (Review)
Review
1. To define the neural circuits activated by dopaminergic stimulation in rat models of parkinsonism, the author studied the effects of L-dopa and selective D1 and D2 agonists on RCGU in rats with unilateral 6-OHDA substantia nigra lesions. 2. Systemic administration of L-dopa markedly increased RCGU in the EP and SNr ipsilateral to the nigral lesions; it is suggested that this represents metabolic activity primarily in axon terminals of GABAergic striatal projection neurons. These effects were reproduced by selective D1, but not D2, dopamine agonists, and were blocked completely by a D1 antagonist, indicating their critical dependence on D1 stimulation. L-dopa moderately increased RCGU in the STN; this effect was reproduced by D1 and D2 agonists and was blocked completely only by combined D1 and D2 antagonist pretreatment. 3. The RCGU data support a direct stimulatory action of dopamine, formed from L-dopa, on D1 receptor-bearing striatal GABAergic neurons projecting to the EP and SNr as well as a net stimulatory action on the GP output to the STN. 4. The marked D1-mediated RCGU increase in the SNr ipsilateral to the dopamine depletion contrasts with the modest increase seen on the contralateral side and in naive rats, suggesting that the enhanced RCGU response to D1 stimulation is an index of dopaminergic supersensitivity. The stimulatory effect of the D1 agonist SKF 38393 on RCGU in the SNr is enhanced 6-12 hours after acute dopamine depletion with reserpine/AMPT indicating that supersensitive responses develop within this rapid time frame. 5. The RCGU data indicate that D1 receptor stimulation contributes importantly, in an anatomically selective manner, to the effects of L-dopa on basal ganglia circuits and that the response to D1 stimulation is rapidly modifiable by dopamine depletion.
Topics: Animals; Autoradiography; Deoxyglucose; Levodopa; Radioligand Assay; Receptors, Dopamine D1; Receptors, Dopamine D2
PubMed: 8539420
DOI: 10.1016/0278-5846(95)00132-f -
Acta Crystallographica. Section C,... Oct 2007The beta-pyranose form, (III), of 3-deoxy-D-ribo-hexose (3-deoxy-D-glucose), C(6)H(12)O(5), crystallizes from water at 298 K in a slightly distorted (4)C(1) chair...
The beta-pyranose form, (III), of 3-deoxy-D-ribo-hexose (3-deoxy-D-glucose), C(6)H(12)O(5), crystallizes from water at 298 K in a slightly distorted (4)C(1) chair conformation. Structural analyses of (III), beta-D-glucopyranose, (IV), and 2-deoxy-beta-D-arabino-hexopyranose (2-deoxy-beta-D-glucopyranose), (V), show significantly different C-O bond torsions involving the anomeric carbon, with the H-C-O-H torsion angle approaching an eclipsed conformation in (III) (-10.9 degrees ) compared with 32.8 and 32.5 degrees in (IV) and (V), respectively. Ring carbon deoxygenation significantly affects the endo- and exocyclic C-C and C-O bond lengths throughout the pyranose ring, with longer bonds generally observed in the monodeoxygenated species (III) and (V) compared with (IV). These structural changes are attributed to differences in exocyclic C-O bond conformations and/or hydrogen-bonding patterns superimposed on the direct (intrinsic) effect of monodeoxygenation. The exocyclic hydroxymethyl conformation in (III) (gt) differs from that observed in (IV) and (V) (gg).
Topics: Crystallography, X-Ray; Deoxyglucose; Hydrogen Bonding; Models, Molecular; Molecular Structure
PubMed: 17917226
DOI: 10.1107/S0108270107038553