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Nature Sep 1962
Topics: DNA
PubMed: 13859584
DOI: 10.1038/195998a0 -
British Medical Bulletin Jan 1962
Topics: DNA; Humans
PubMed: 13875073
DOI: 10.1093/oxfordjournals.bmb.a069930 -
Analytical Sciences : the International... Aug 2019This paper describes a deoxyribonucleic-acid-sensitive electrolyte solution-gate field-effect transistor (SGFET) sensor utilizing a partial carboxyl-terminated...
This paper describes a deoxyribonucleic-acid-sensitive electrolyte solution-gate field-effect transistor (SGFET) sensor utilizing a partial carboxyl-terminated boron-doped polycrystalline diamond surface as a linker to connect a deoxyribonucleic acid (DNA) probe. A high density of carboxyl termination on the polycrystalline diamond surface that was employed as a FET channel was achieved using a vacuum ultraviolet system with oxygen gas. A single-stranded DNA probe was immobilized on the polycrystalline diamond channel via amino coupling. The current-voltage characteristics of the polycrystalline diamond SGFET sensor was examined with bias voltages within its potential voltage window. The characteristics of the drain-source current verses the drain-source voltage showed a pinch-off, a shift voltage of up to 40 mV with a coefficient of variation of 4 - 11% was obtained between hybridization and denaturation. In addition, a single nucleotide mutation of DNA sequence was selectively recognized by the shift voltage up to ca. 10 mV.
Topics: Boron; Crystallization; DNA; Diamond; Nucleic Acid Hybridization; Polymers; Solutions; Transistors, Electronic
PubMed: 31061238
DOI: 10.2116/analsci.18P520 -
British Medical Bulletin Sep 1965
Review
Topics: DNA; In Vitro Techniques; Molecular Biology; RNA
PubMed: 5317916
DOI: 10.1093/oxfordjournals.bmb.a070395 -
Bacteriological Reviews Jun 1970
Topics: Ascomycota; Basidiomycota; Biological Evolution; Centrifugation, Density Gradient; Cytosine; DNA; Fungi; Guanine; Mitosporic Fungi; Statistics as Topic; Yeasts
PubMed: 5528264
DOI: 10.1128/br.34.2.126-154.1970 -
Molecules (Basel, Switzerland) Feb 2020Deoxyribonucleic acid (DNA) serves not only as a genetic information carrier but also as an excellent material for programmable nanoscale assembly [...].
Deoxyribonucleic acid (DNA) serves not only as a genetic information carrier but also as an excellent material for programmable nanoscale assembly [...].
Topics: DNA; Nanostructures; Nanotechnology
PubMed: 32028556
DOI: 10.3390/molecules25030639 -
Journal of Biomolecular Structure &... Jul 2019The paper represents the study of interaction between deoxyribonucleic acid (DNA) and deoxyribonucleic acid-binding protein from starved cells (DPS) cluster formation...
Interaction of deoxyribonucleic acid with deoxyribonucleic acid-binding protein from starved cells: cluster formation and crystal growing as a model of initial stages of nucleoid biocrystallization.
The paper represents the study of interaction between deoxyribonucleic acid (DNA) and deoxyribonucleic acid-binding protein from starved cells (DPS) cluster formation and crystal growing within a cell. This study is a part of the project that includes European Synchrotron Radiation Facility (ESRF) investigations of in vivo and in vitro nanocrystallization processes of Escherichia coli (E. coli) nucleoid under stress condition combined with theoretical molecular dynamics approaches. Nucleoid biocrystallization is an adaptive mechanism of bacterial cells under stress. It is poorly understood at the present time. Understanding crystal formation process is a very important for molecular biology, pharmacology and other areas. In the simulation part the coarse-grained modeling of various combinations of the following molecules was used: DPS proteins (from 1 to 108 DPS dodecamers in simulation box), short DNA fragments with a length of 24 base pairs (b.p., from 1 to 100 DNA fragments in simulation box) and a part of pBluescript SK(+) plasmide with a length of 161 b.p., in the presence of ions. We defined structural, energetic and dynamic properties of DPS-DPS and DPS-DNA complexes in clusters and crystals that allow us to predict crystal formation and the structure of these crystals in experimental systems. Communicated by Ramaswamy H. Sarma.
Topics: Algorithms; DNA; DNA-Binding Proteins; Humans; Molecular Conformation; Molecular Docking Simulation; Molecular Dynamics Simulation; Protein Binding
PubMed: 30033848
DOI: 10.1080/07391102.2018.1492458 -
Journal of Molecular Graphics &... Dec 2019Metal ion interaction with deoxyribonucleic acid and peptide nucleic acid were studied using B3LYP-D3/6-311++g(d,p)//B3LYP/6-31 + G(d) level of theory in aqueous phase...
Metal ion interaction with deoxyribonucleic acid and peptide nucleic acid were studied using B3LYP-D3/6-311++g(d,p)//B3LYP/6-31 + G(d) level of theory in aqueous phase employing polarized continuum (PCM) model. This study reports the role of backbones on deoxyribonucleic acid and peptide nucleic acid for complexation with different metal ions. The systematic study performed with DFT calculations reveals that central binding (Type-4) shows the strongest binding compared to the other binding modes because of the involvement of the backbone as well as the nitrogenous bases. The charged backbone of DNA nucleotides contributes significantly towards binding with the metal ions. The deoxyguanosine monophosphate (dGMP) clearly indicates the strongest binding upon complexation with Mg (-49.6 kcal/mol), Zn (-45.3 kcal/mol) and Cu (-148.4 kcal/mol), respectively. The neutral backbone of PNA also assists to complex the metal ions with PNA nucleotides. The Mg and Cu prefer to bind with the PNA-Cytosine (-32.9 kcal/mol & -132.9 kcal/mol) in central binding mode (type-4). PNA-Adenine-Zn (-29.1 kcal/mol) is the preferred binding mode (type-4) compared to other modes of interaction for this metal ion with PNA-Adenine nucleotide. The Cu ion showed the superior complexation ability with deoxyribonucleic acid and peptide nucleic acid compared to Mg and Zn ions. The cation-π complexation with the bases of nucleotides was also obtained with Cu ion. The AIM (atoms in molecule) theory has been applied to examine the nature of the interaction of Mg, Zn, and Cu ion to the deoxyribonucleic acid and peptide nucleic acid. The alkaline earth metal, Mg ion shows electrostatic nature while interaction with deoxyribonucleic acid and peptide nucleic acid, however, the transition metal ions (Zn, Cu) showed partly covalent nature as well with deoxyribonucleic acid and peptide nucleic acid. The optical properties calculated for the binding of metal ions with deoxyribonucleic acid and peptide nucleic acid showed a diagnostic signature to ascertain the interaction of metal ions with such nucleotides. Cu ion showed larger red shifts in the absorption spectrum values upon complexation with the DNAs and PNAs. The calculated results suggest that such metal ions would prefer to bind with the DNA compared to PNA in DNA-PNA duplexes. The preference for the binding of metal ions with DNA nucleotides is largely attributed to the contribution of charged backbones compared to the neutral PNA backbones.
Topics: Copper; DNA; Molecular Structure; Peptide Nucleic Acids; Zinc
PubMed: 31494536
DOI: 10.1016/j.jmgm.2019.107445 -
Otology & Neurotology : Official... Sep 2002Contamination of archival human temporal bones with extraneous deoxyribonucleic acid may represent a potentially significant problem in the analysis of nucleic acids...
HYPOTHESIS
Contamination of archival human temporal bones with extraneous deoxyribonucleic acid may represent a potentially significant problem in the analysis of nucleic acids isolated from archival specimens.
BACKGROUND
During the past decade, there has been growing interest in the development of molecular biologic techniques that can be applied to the investigation of pathologic changes in archival human temporal bones. The impetus for the development of these techniques is in part related to the fact that the temporal bone collections represent a repository of archival material compiled over decades, which is not available from living patients.
METHODS
An archival human temporal bone specimen from a male patient with the Mohr-Tranebjaerg syndrome (formerly called DFN-1) and a well-characterized mutation was analyzed for the presence of the mutation by a standard method for extraction, isolation, amplification, and sequencing of deoxyribonucleic acid. The experiment was repeated four times.
RESULTS
The deoxyribonucleic acid sequence from three of four extractions was normal. The known mutation was easily and repeatedly demonstrated in a blood sample from the same individual. Because Mohr-Tranebjaerg syndrome is X-linked, there is only one allele, and therefore there is no potential endogenous source to account for the normal sequence that was amplified. Contamination of the tissue sections by extraneous deoxyribonucleic acid presumably occurred during acquisition and processing of the temporal bone.
CONCLUSIONS
Contamination of archival temporal bones with exogenous deoxyribonucleic acid is a significant potential problem that must be considered in the interpretation of the results of deoxyribonucleic acid retrieved from archival sections. The authors recommend collecting blood samples from temporal bone donors in the future to ensure the availability of a reliable source of deoxyribonucleic acid.
Topics: Aged; Archives; Culture Techniques; DNA; DNA Damage; DNA Mutational Analysis; Humans; Male; Nucleic Acid Amplification Techniques; Orofaciodigital Syndromes; Temporal Bone
PubMed: 12218636
DOI: 10.1097/00129492-200209000-00030 -
Nature Nov 1965