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The Journal of Biological Chemistry Jul 1958
Topics: DNA; Escherichia coli
PubMed: 13563462
DOI: No ID Found -
Journal of Bacteriology Sep 1963Young, F. E. (Western Reserve University Cleveland, Ohio) and John Spizizen. Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system. J....
Young, F. E. (Western Reserve University Cleveland, Ohio) and John Spizizen. Incorporation of deoxyribonucleic acid in the Bacillus subtilis transformation system. J. Bacteriol. 86:392-400. 1963.-The optimal conditions for the incorporation of deoxyribonucleic acid (DNA) were studied. In competent cells, the irreversible binding of DNA was influenced by temperature, hydrogen ion concentration, and aeration. Divalent cations, such as barium, strontium, calcium, or magnesium, were required. Under suboptimal environmental conditions and with metabolic inhibitors, the process of transformation was decreased to a greater extent than was incorporation of DNA. Under conditions of phosphate depletion, the incorporation of P(32) increased. However, the frequency of transformation decreased. This inducible process was not related to competence.
Topics: Bacillus subtilis; DNA; DNA, Bacterial; Hydrogen-Ion Concentration; Magnesium; Research; Temperature
PubMed: 14066414
DOI: 10.1128/jb.86.3.392-400.1963 -
Nature Dec 1961
Topics: DNA; Solutions
PubMed: 13889705
DOI: 10.1038/192963a0 -
The Biochemical Journal Dec 1972Previous investigations on the persistence length of DNA in solution have revealed large discrepancies between hydrodynamic results and those from light-scattering...
Previous investigations on the persistence length of DNA in solution have revealed large discrepancies between hydrodynamic results and those from light-scattering techniques which have potentially a greater resolving power. The information obtained from experiments on a small circular DNA molecule has resolved these discrepancies. The non-superhelical circular double-stranded DNA molecule from bacteriophage [unk]X174-infected cells is small enough to permit accurate light-scattering extrapolations, and its solutions have negligible anisotropy. The persistence length obtained from experimental investigations on this molecule is comparable with that obtained by hydrodynamic techniques, even with variation of the excluded-volume factor.
Topics: Bacteriophages; Chemical Phenomena; Chemistry; DNA, Circular; DNA, Viral; Light; Mathematics; Molecular Weight; Scattering, Radiation; Solutions
PubMed: 4656791
DOI: 10.1042/bj1301019 -
The Journal of Biological Chemistry Jun 1962
Topics: Base Sequence; Cluster Analysis; DNA
PubMed: 13918810
DOI: No ID Found -
Journal of Molecular Biology Jul 1962
Topics: Base Composition; DNA; Temperature
PubMed: 14470099
DOI: 10.1016/s0022-2836(62)80066-7 -
Cold Spring Harbor Symposia on... 1961
Topics: Bacteria; Ciliophora; DNA; Nucleoproteins; Proteins
PubMed: 13918160
DOI: 10.1101/sqb.1961.026.01.009 -
The Biochemical Journal May 19691. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a...
1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 10(8) daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3x10(6) and 1.2x10(8) daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.
Topics: Cell Nucleus; Centrifugation, Density Gradient; Chloroplasts; DNA; DNA, Bacterial; DNA, Viral; Densitometry; Kinetics; Methods; Microscopy, Electron; Mitochondria; Molecular Weight; Nucleic Acid Denaturation; Plant Cells; Plants; Purines; Pyrimidinones; Species Specificity; Time Factors
PubMed: 5821734
DOI: 10.1042/bj1120777 -
Journal of Bacteriology Jan 1977The structural gene for ampicillin resistance resides upon a 3.2 X 10(6)-dalton sequence of deoxyribonucleic acid, TnA that can be transposed from replicon to replicon...
The structural gene for ampicillin resistance resides upon a 3.2 X 10(6)-dalton sequence of deoxyribonucleic acid, TnA that can be transposed from replicon to replicon in laboratory experiments. TnA was transposed from a large conjugative plasmid to a small nonconjugative plasmid, RSF1010. Several RSF1010::TnA plasmids isolated in these laboratory experiments have been shown to be identical to plasmids found in clinical isolates. These data provide direct support to the theory that transposition of drug resistance genes play a key role in the evolution of R plasmids.
Topics: Ampicillin; Conjugation, Genetic; DNA, Bacterial; DNA, Circular; DNA, Recombinant; Enterobacteriaceae; Penicillin Resistance; R Factors; Recombination, Genetic
PubMed: 583751
DOI: 10.1128/jb.129.1.530-533.1977 -
Archives of Biochemistry and Biophysics Jan 1962
Topics: Acridine Orange; Acridines; DNA; Humans
PubMed: 13872209
DOI: 10.1016/0003-9861(62)90448-4