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Journal of Bacteriology Nov 1979Plasmid and phage deoxyribonucleic acid (DNA) harboring bacterial insertion sequence (IS) elements IS1, IS2, and IS5 were characterized and used as probes to detect...
Plasmid and phage deoxyribonucleic acid (DNA) harboring bacterial insertion sequence (IS) elements IS1, IS2, and IS5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. The hybridization method used permits the detection of sequences partially homologous to the elements. Hybridization of the IS-containing probes to each other revealed a region of limited homology between IS1 and IS2. Homologous sequences were then detected by computer analysis of the published IS1 and IS2 nucleotide sequences. The homologous sequence contains a tandemly repeated tetranucleotide sequence which resembles the repeated sequence at the hot spot for spontaneous mutations in the lacI gene (P. J. Farabaugh, U. Schmeissner, M. Hofer, and J. Miller, J. Mol. Biol. 126:847-863, 1978). Homology between the IS elements and various genomes was determined by hybridizing labeled DNA containing IS1, IS2, and IS5 sequences to Southern blots of chromosomal DNA cleaved with restriction endonucleases. IS1 and IS5 appear limited to the enteric bacteria, whereas IS2 sequences can also be detected in Pseudomonas putida, Pseudomonas aeruginosa, and Serratia marcescens. Bacteria which appear not to possess extrachromosomal elements, e.g., Caulobacter crescentus, did not show homology with any insertion sequences tested. In addition, sequences homologous to IS1, IS2, or IS5 were not detected in Saccharomyces cerevisiae, Dictyostelium discoideum, or calf thymus DNA.
Topics: Bacteria; Bacteriophage lambda; Base Sequence; Computers; DNA; DNA Transposable Elements; DNA, Bacterial; DNA, Fungal; Nucleic Acid Hybridization; Plasmids
PubMed: 159291
DOI: 10.1128/jb.140.2.588-596.1979 -
Science (New York, N.Y.) Jul 1958
Topics: Blood Cells; Cell Division; DNA; Humans
PubMed: 13568764
DOI: 10.1126/science.128.3317.202 -
Nature Apr 1963
Topics: DNA; DNA, Neoplasm; Neoplasms; Nuclear Proteins; Nucleoproteins; RNA; RNA, Neoplasm
PubMed: 13987611
DOI: 10.1038/198147a0 -
Biochimica Et Biophysica Acta Sep 1964
Topics: Bacillus subtilis; Biophysical Phenomena; DNA; DNA, Bacterial; Hydrogen-Ion Concentration; Research; Temperature
PubMed: 14227280
DOI: 10.1016/0926-6550(64)90171-9 -
The Analyst Apr 1994Tandem mass spectrometry (MS-MS) has proved to be a state-of-the-art technique for the structure of synthetic and biological compounds. One opportunity for MS-MS is the... (Review)
Review
Tandem mass spectrometry (MS-MS) has proved to be a state-of-the-art technique for the structure of synthetic and biological compounds. One opportunity for MS-MS is the study of modified deoxyribonucleic acid (DNA) bases resulting from the attachment of carcinogens such as polycyclic aromatic hydrocarbons (PAHs). The determination of PAH-DNA adducts, formed in vivo via a one-electron oxidation or a diol-epoxide mechanism, requires high efficiency separation and very sensitive techniques. This is because the analyte will occur in complex biological mixtures and at low femtomole levels, considering that one modification occurs for 10(6) or 10(8) bases. This paper reviews various approaches to the separation and mass spectrometric structural determination of DNA adducts. The main emphasis of our research is the sub-picomolar detection and identification of DNA-PAH adducts, particularly those formed via a one-electron oxidation mechanism.
Topics: Animals; DNA; DNA Damage; Mammals; Mass Spectrometry; Polycyclic Compounds
PubMed: 8024117
DOI: 10.1039/an9941900497 -
Journal of Bacteriology May 1962Randall, Charles C. (University of Mississippi School of Medicine, Jackson), Lanelle G. Gafford, and Robert W. Darlington. Bases of the nucleic acid of fowlpox virus and...
Randall, Charles C. (University of Mississippi School of Medicine, Jackson), Lanelle G. Gafford, and Robert W. Darlington. Bases of the nucleic acid of fowlpox virus and host deoxyribonucleic acid. J. Bacteriol. 83:1037-1041. 1962.-The nucleic acid of fowlpox virus obtained from purified inclusions was identified as deoxyribonucleic acid (DNA). The base ratios determined on whole virus and on isolated DNA were practically identical. The molar ratios of purines to pyrimidines were approximately unity. The ratio of adenine plus thymine to guanine plus cytosine was 1.8. The base ratios of DNA obtained from chick red cells were approximately the same as other animal DNA preparations reported in the literature. It is of interest that the molar proportions of host DNA are significantly different from viral DNA.
Topics: Adenine; DNA; Fowlpox virus; Guanine; Nucleic Acids; Purines; Pyrimidines; Viruses
PubMed: 14490218
DOI: 10.1128/jb.83.5.1037-1041.1962 -
Journal of Bacteriology Oct 1964De Ley, J. (State University, Ghent, Belgium), and S. Friedman. Deoxyribonucleic acid hybrids of acetic acid bacteria. J. Bacteriol. 88:937-945. 1964.-Deuterated...
De Ley, J. (State University, Ghent, Belgium), and S. Friedman. Deoxyribonucleic acid hybrids of acetic acid bacteria. J. Bacteriol. 88:937-945. 1964.-Deuterated N(15)-labeled deoxyribonucleic acid (DNA) from Acetobacter aceti (mesoxydans 4) forms hybrids with ordinary DNA from other species of this genus (A. xylinum, A. pasteurianus, A. estunensis, and possibly A. xylinoides) when the guanine plus cytosine base composition does not vary by more than 1 to 2%. Beyond this limit (A. aceti Ch31 and A. muciparus 5) no hybrids are formed. The hybrids are apparently derived from an asymmetrical part of the compositional distribution. The results lend strength to the concept of a genetic species rather than to a division of a genus into sharply separated species, based on small phenotypic differences. Taxonomic implications are discussed.
Topics: Acetic Acid; Acetobacter; Bacteria; Base Composition; DNA; DNA, Bacterial; Hybridization, Genetic; Nucleic Acid Hybridization; Research
PubMed: 14219057
DOI: 10.1128/JB.88.4.937-945.1964 -
Nature Feb 1963
Topics: Bacillus subtilis; Bacteriophages; DNA; DNA, Viral; Nucleotides; Thymidine Monophosphate; Uracil Nucleotides
PubMed: 13980287
DOI: 10.1038/197794a0 -
Journal of Bacteriology Aug 1961Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent...
Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the presence of DNA + deoxyribonuclease + mixtures of deoxynucleosides and deoxynucleotides (supplement-1) than unsupplemented suspensions. Under identical conditions, avirulent and weakly virulent pneumococci do not respond to these DNA breakdown products. Glucose and casitone must be present for maximal effects to occur.A DNA turnover occurs in virulent cells. This has been demonstrated by a decrease in specific activity of H(3)-DNA extracted from virulent (S) cells exposed to nonradioactive supplement-1.
Topics: DNA; DNA Replication; Deoxyribonucleases; Streptococcus pneumoniae
PubMed: 13699770
DOI: 10.1128/jb.82.2.169-180.1961 -
The Journal of Biological Chemistry Oct 1959
Topics: DNA; DNA Primers
PubMed: 13802337
DOI: No ID Found