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Analytica Chimica Acta Oct 2014A novel enzyme-free amplification strategy was designed for sensitive electrochemical detection of deoxyribonucleic acid (DNA) based on Zn(2+) assistant DNA recycling...
Assistant deoxyribonucleic acid recycling with Zn(2+) and molecular beacon for electrochemical detection of deoxyribonucleic acid via target-triggered assembly of mutated DNAzyme.
A novel enzyme-free amplification strategy was designed for sensitive electrochemical detection of deoxyribonucleic acid (DNA) based on Zn(2+) assistant DNA recycling via target-triggered assembly of mutated DNAzyme. A gold electrode was used to immobilize molecular beacon (MB) as the recognition probe and perform the amplification procedure. In the presence of target DNA, the hairpin probe 1 was opened, and the DNAzyme was liberated from the caged structure. The activated DNAzyme first hybridized and then cleaved the MB in the presence of cofactor Zn(2+). After cleavage, the MB was cleaved into two pieces and the ferrocene (Fc) labeled piece dissociated from the gold electrode, thus obviously decreasing the Fc signal and forming a free DNAzyme strand. Finally, each target-induced activated DNAzyme underwent many cycles to trigger the cleavage of many MB substrates. Therefore, the peak current of Fc dramatically decreased to approximately zero. The strategy showed a detection limit at 35 fM levels, which was about 2 orders of magnitude lower than that of the conventional hybridization without Zn(2+)-based amplification. The Zn(2+) assistant DNA recycling offers a versatile platform for DNA detection in a cost-effective manner, and has a promising application in clinical diagnosis.
Topics: DNA; DNA, Catalytic; Electrochemical Techniques; Electrodes; Gold; Mutation; Zinc
PubMed: 25201265
DOI: 10.1016/j.aca.2014.07.013 -
Cold Spring Harbor Symposia on... 1956
Topics: DNA; Micrococcus
PubMed: 13433579
DOI: 10.1101/sqb.1956.021.01.004 -
The Journal of Biological Chemistry Mar 1961
Topics: Base Sequence; Cluster Analysis; DNA
PubMed: 13790780
DOI: No ID Found -
Science (New York, N.Y.) Sep 1960
Topics: DNA; DNA Replication
PubMed: 13795013
DOI: 10.1126/science.132.3427.617 -
The Biochemical Journal Mar 1962
Topics: DNA; DNA Primers; DNA-Directed DNA Polymerase; RNA
PubMed: 14454860
DOI: 10.1042/bj0820493 -
Saudi Medical Journal Feb 2011
Topics: DNA; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Humans; Lupus Erythematosus, Systemic; Sensitivity and Specificity
PubMed: 21301775
DOI: No ID Found -
Journal of Bacteriology Jul 1959
Topics: DNA; Thymidine
PubMed: 13672916
DOI: 10.1128/jb.78.1.110-113.1959 -
The Journal of Biological Chemistry Aug 1962
Topics: DNA; DNA-Directed RNA Polymerases; Endonucleases; RNA
PubMed: 13895983
DOI: No ID Found -
The Journal of Biological Chemistry Jan 1964
Topics: Chromatography; DNA; DNA, Bacterial; DNA-Directed DNA Polymerase; Electrophoresis; Escherichia coli; Metabolism; Nucleotidases; Phosphorus Isotopes; Research
PubMed: 14114848
DOI: No ID Found -
The Journal of Biological Chemistry Aug 1956
Topics: DNA; DNA Replication; Digestion; Liver
PubMed: 13357466
DOI: No ID Found