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Virology Jul 1995Goose parvovirus is the etiological agent of Derzsy's disease, a fatal hepatitis of young geese. The virus infects geese and Muscovy ducks and can be propagated in the...
Goose parvovirus is the etiological agent of Derzsy's disease, a fatal hepatitis of young geese. The virus infects geese and Muscovy ducks and can be propagated in the laboratory in primary embryonic goose fibroblasts. To date the virus has only been classified by morphological, biochemical, and culture characteristics as an autonomous parvovirus. We now report the cloning and partial sequencing of 3434 nucleotides of the viral genome. Three overlapping clones were obtained, encoding regions in the nonstructural and capsid coding region. The nucleotide sequence show little homology to other autonomous parvoviruses but 55% homology to the dependovirus AAV2. The homology to AAV2 was also confirmed at the amino acid level (nonstructural protein 55%, capsid coding region 51%). DNA cross hybridization studies indicate an even closer similarity of goose parvovirus to the yet unsequenced human dependoviruses AAV1 and AAV3 than to AAV2. These findings suggest that goose parvovirus may be genetically related to the dependovirus genus rather than to the other autonomous parvoviruses.
Topics: Amino Acid Sequence; Animals; Base Sequence; Capsid; Cloning, Molecular; DNA, Single-Stranded; DNA, Viral; Dependovirus; Geese; Genome, Viral; Molecular Sequence Data; Nucleic Acid Hybridization; Parvovirus; Polymerase Chain Reaction; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Viral Nonstructural Proteins
PubMed: 7618268
DOI: 10.1006/viro.1995.1345 -
Methods in Molecular Biology (Clifton,... 2011Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated...
Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated by early studies that generated infectious molecular clones, determined the sequence of the genome, and defined the genetic elements of the virus. The refinement of methods and protocols for the production and application of rAAV vectors has come from years of studies that explored the basic biology of this virus and its interaction with host cells. Interest in improving vector performance has in turn driven studies that have provided tremendous insights into the basic biology of the AAV lifecycle. In this chapter, we review the background on AAV biology and its exploitation for vectors and gene delivery.
Topics: Dependovirus; Gene Transfer Techniques; Genetic Vectors; Serotyping; Virus Replication
PubMed: 22034024
DOI: 10.1007/978-1-61779-370-7_1 -
Current Opinion in Biotechnology Oct 1992Adeno-associated virus is a human parvovirus that integrates its DNA genome into host cell chromosomes with very high efficiency. This suggests that adeno-associated... (Review)
Review
Adeno-associated virus is a human parvovirus that integrates its DNA genome into host cell chromosomes with very high efficiency. This suggests that adeno-associated virus may be a useful vector for human gene therapy. Interest in adeno-associated virus vectors increased greatly in the last year following reports that adeno-associated virus genome integration may be site specific and occur at preferred sites in the human genome. Several genes relevant to the treatment of genetic or infectious diseases have been expressed in adeno-associated virus vectors in vitro.
Topics: Biotechnology; Dependovirus; Genetic Therapy; Genetic Vectors; Genome, Viral; Humans; Safety; Virus Integration
PubMed: 1369403
DOI: 10.1016/0958-1669(92)90082-t -
Neuroscience Bulletin May 2023
Topics: Dependovirus; Microglia; Cells, Cultured; Transduction, Genetic
PubMed: 36333483
DOI: 10.1007/s12264-022-00975-x -
Current Opinion in Virology Dec 2016In this review, a brief account of the historical perspective of the discovery of the first cellular receptor and co-receptor of the prototype adeno-associated virus... (Review)
Review
In this review, a brief account of the historical perspective of the discovery of the first cellular receptor and co-receptor of the prototype adeno-associated virus serotype 2 (AAV2) will be presented. The Subsequent discovery of a number of AAV serotypes, and attempts to identify the cellular receptors and co-receptors for these serotype vectors has had significant implications in their use in human gene therapy. As additional AAV serotypes are discovered and isolated, a detailed understanding of their tropism is certainly likely to play a key role in all future studies, both basic science as well as clinical.
Topics: Dependovirus; Genetic Vectors; Humans; Receptors, Virus; Viral Tropism; Virus Attachment; Virus Internalization
PubMed: 27596608
DOI: 10.1016/j.coviro.2016.08.003 -
Current Opinion in Virology Jun 2017Viral vectors based on adeno-associated virus (AAV) are leading candidates for therapeutic gene delivery. Understanding rate-limiting steps in the entry of AAV vectors... (Review)
Review
Viral vectors based on adeno-associated virus (AAV) are leading candidates for therapeutic gene delivery. Understanding rate-limiting steps in the entry of AAV vectors may be used in a rational approach to improve efficiency and specificity of transduction. This review describes our current understanding of AAV entry, a key step during infection. We discuss the identity and functions of AAV receptors and attachment factors, including the recently discovered multi-serotype receptor AAVR. We further provide an overview of other host factors that act during the trafficking stage of AAV vector transduction. In particular, we focus on cellular protein complexes associated with retrograde transport from endosomes to the trans-Golgi network. The novel insights in AAV-host interactions facilitated by technological advances in genetic screening approaches provide a greater depth in our understanding how AAV vectors exploit host factors to deliver its genetic cargo to the nucleus.
Topics: Animals; Biological Transport; Dependovirus; Genetic Vectors; Golgi Apparatus; Host-Pathogen Interactions; Humans; Mice; Polysaccharides; Serogroup; Transduction, Genetic; Virus Internalization
PubMed: 28672171
DOI: 10.1016/j.coviro.2017.06.003 -
Journal of Biosciences Sep 2019The success of viral vectors mediated gene therapy is still hampered by immunogenicity and insufficient transgene expression. Alternatively, non-viral vectors mediated... (Review)
Review
The success of viral vectors mediated gene therapy is still hampered by immunogenicity and insufficient transgene expression. Alternatively, non-viral vectors mediated gene delivery has the advantage of low immunogenicity despite showing low transgene expression. By carefully considering the advantages of each approach, hybrid vectors are currently being developed by modifying the viral vectors using non-viral biopolymers. This review provides an overview of the hybrid vectors currently being developed.
Topics: Animals; Biopolymers; Dependovirus; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Humans; Transgenes
PubMed: 31502562
DOI: No ID Found -
Methods in Molecular Biology (Clifton,... 2021Virotherapy, enabled by recent advances in the transdisciplinary field of biotechnology, has emerged as a powerful tool for use in anticancer treatment, gene therapy,...
Virotherapy, enabled by recent advances in the transdisciplinary field of biotechnology, has emerged as a powerful tool for use in anticancer treatment, gene therapy, immunotherapy, etc. Examining the effects of viruses and virus-derived immune-modulating therapeutics is of great fundamental and clinical interest. Here we describe a sample preparation protocol for metabolite extraction from virus-infected tissue, in addition to liquid chromatography-mass spectrometry conditions essential for subsequent analysis. This metabolomics approach delivers highly sensitive and specific metabolite information on various biospecimens. Such an approach may be adopted to monitor biological changes in over 30 relevant metabolic pathways in response to viral infection and also viral therapeutics.
Topics: Animals; Chromatography, Liquid; Dependovirus; Humans; Metabolic Networks and Pathways; Metabolome; Metabolomics; Neoplasms; Oncolytic Virotherapy; Tandem Mass Spectrometry
PubMed: 33108663
DOI: 10.1007/978-1-0716-1012-1_10 -
Human Gene Therapy Feb 2017
Topics: Capsid; Dependovirus; Genetic Vectors; Humans
PubMed: 28186848
DOI: 10.1089/hum.2017.29039.trf -
Microbiology Spectrum Aug 2015In the nearly five decades since its accidental discovery, adeno-associated virus (AAV) has emerged as a highly versatile vector system for both research and clinical... (Review)
Review
In the nearly five decades since its accidental discovery, adeno-associated virus (AAV) has emerged as a highly versatile vector system for both research and clinical applications. A broad range of natural serotypes, as well as an increasing number of capsid variants, has combined to produce a repertoire of vectors with different tissue tropisms, immunogenic profiles and transduction efficiencies. The story of AAV is one of continued progress and surprising discoveries in a viral system that, at first glance, is deceptively simple. This apparent simplicity has enabled the advancement of AAV into the clinic, where despite some challenges it has provided hope for patients and a promising new tool for physicians. Although a great deal of work remains to be done, both in studying the basic biology of AAV and in optimizing its clinical application, AAV vectors are currently the safest and most efficient platform for gene transfer in mammalian cells.
Topics: Animals; Dependovirus; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Humans; Mammals
PubMed: 26350320
DOI: 10.1128/microbiolspec.MDNA3-0052-2014