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Journal of Clinical Microbiology Sep 2001Dermabacter hominis was the cause of a peritoneal dialysis-associated peritonitis. D. hominis was identified by phenotypic criteria and by sequencing the 16S rRNA gene....
Dermabacter hominis was the cause of a peritoneal dialysis-associated peritonitis. D. hominis was identified by phenotypic criteria and by sequencing the 16S rRNA gene. Clinical cure was achieved with cefuroxime treatment despite the isolate's reduced susceptibility to this drug (MIC, 12 mg/liter) on in vitro testing. The successful treatment was probably due to the high concentrations attained by intraperitoneal administration of the drug.
Topics: Actinomycetales; Actinomycetales Infections; Aged; Female; Humans; Molecular Sequence Data; Peritoneal Dialysis; Peritonitis
PubMed: 11526195
DOI: 10.1128/JCM.39.9.3420-3421.2001 -
New Microbes and New Infections May 2016Strain FF11(T) was isolated from the wound on a researcher's finger who had been bitten by a fish (Protopterus annectens) in Senegal. Analysis by matrix-assisted laser...
Strain FF11(T) was isolated from the wound on a researcher's finger who had been bitten by a fish (Protopterus annectens) in Senegal. Analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry did not provide any identification, but the 16S rRNA sequence exhibited 97.9% identity with Dermabacter hominis. Phenotypic and genomic analyses demonstrated that strain FF11(T) is Gram-positive, facultatively anaerobic, nonmotile and non-spore forming; it exhibited a genome of 2 222 902 bp encoding 2074 protein-coding and 50 RNA genes, with a 63.2% G+C content. We consequently proposed the creation of Dermabacter indicis strain FF11(T).
PubMed: 27081494
DOI: 10.1016/j.nmni.2016.02.007 -
International Journal of Systematic and... Jul 2016A Gram-stain-positive, catalase-positive, facultatively anaerobic, non-motile, coryneform bacterium, designated strain 32, was isolated from a closed pus sample from a...
A Gram-stain-positive, catalase-positive, facultatively anaerobic, non-motile, coryneform bacterium, designated strain 32, was isolated from a closed pus sample from a patient having finger necrosis in Korea. Strain 32 was considered as representing a novel species according to its initial identification by matrix-assisted laser desorption/ionization-time-of-flight MS. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 32 belonged to the genus Dermabacter and was closely related to Dermabacter hominis DSM 7083 (=ATCC 49369) (98.34 % similarity). Optimal growth was observed at 30-40 °C and pH 7. Growth occurred in the presence of 0-6 % (w/v) NaCl. Menaquinones MK-8, MK-7 and MK-9 were the major respiratory quinones. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, glycolipid and two unknown lipids. The major cellular fatty acids were anteiso-C17 : 0, anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0. The DNA G+C content of strain 32 was 62.58 mol%, and the mean level of DNA-DNA relatedness between strain 32 and D. hominis ATCC 49369 was 49±1.6 %. Based on the phenotypic and genotypic characteristics, strain 32 is confirmed to represent a novel species of the genus Dermabacter, for which the name Dermabacter jinjuensis sp. nov. is proposed. The type strain is 32 (=NCCP 16133=DSM 101003).
Topics: Actinomycetales; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Fingers; Humans; Necrosis; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA; Suppuration; Vitamin K 2
PubMed: 27088668
DOI: 10.1099/ijsem.0.001092 -
Anaerobe Oct 2009A case of recurrent abscesses in an immunocompetent patient is reported, involving the opportunistic human pathogen Dermabacter hominis, the virulent anaerobic pathogen...
A case of recurrent abscesses in an immunocompetent patient is reported, involving the opportunistic human pathogen Dermabacter hominis, the virulent anaerobic pathogen Finegoldia magna and Staphylococcus aureus.
Topics: Abscess; Actinomycetales; Adult; Gram-Positive Bacteria; Gram-Positive Bacterial Infections; Humans; Male; Recurrence; Staphylococcus aureus
PubMed: 19332143
DOI: 10.1016/j.anaerobe.2009.03.006 -
European Journal of Clinical... Jun 2002
Topics: Actinomyces; Actinomycetales; Actinomycetales Infections; Aged; Chronic Disease; Humans; Male; Osteomyelitis
PubMed: 12111611
DOI: 10.1007/s10096-002-0747-8 -
International Journal of Systematic... Jul 199416S rRNA gene sequencing studies were performed on Dermabacter hominis and four meso-diaminopimelic acid-containing species of the genus Brevibacterium. Phylogenetic...
16S rRNA gene sequencing studies were performed on Dermabacter hominis and four meso-diaminopimelic acid-containing species of the genus Brevibacterium. Phylogenetic analysis revealed a close association between Dermabacter hominis and representatives of the lysine-containing genera Arthrobacter, Micrococcus, and Renibacterium. By contrast, the genus Brevibacterium formed a distinct line of descent within the high-guanine-plus-cytosine-containing actinomycetes, displaying no specific affinity with any other organism examined.
Topics: Actinomycetales; Base Composition; Brevibacterium; Diaminopimelic Acid; Molecular Sequence Data; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Species Specificity
PubMed: 7520745
DOI: 10.1099/00207713-44-3-583 -
Journal of Clinical Microbiology May 1994Over a 1-year period, 11 isolates (including 5 from blood cultures) of the recently described CDC group 3 and group 5 coryneform bacteria were derived from clinical...
Over a 1-year period, 11 isolates (including 5 from blood cultures) of the recently described CDC group 3 and group 5 coryneform bacteria were derived from clinical specimens and compared with reference strains. Biochemical characteristics indicated a very close relationship between CDC group 3 and group 5 coryneform bacteria. The ability of CDC group 3 and the inability of CDC group 5 coryneform bacteria to ferment xylose were the only reactions that were different for the two taxa. Chemotaxonomic features of the two groups included the presence of meso-diaminopimelic acid, a lack of mycolic acids, and the presence of predominantly branched cellular fatty acids, a combination found among gram-positive rods only in Brevibacterium spp., Brachybacterium faecium, and Dermabacter hominis. 16S rRNA gene sequence analysis revealed that CDC group 3 and group 5 coryneform bacteria are members of the genus Dermabacter, which to date has been isolated exclusively from human skin.
Topics: Actinomycetales; Actinomycetales Infections; Bacteremia; Bacterial Typing Techniques; Base Sequence; DNA, Bacterial; DNA, Ribosomal; Fermentation; Humans; Molecular Sequence Data; Species Specificity; Xylose
PubMed: 8051248
DOI: 10.1128/jcm.32.5.1223-1228.1994 -
International Journal of Cosmetic... Jun 2024This study aimed to expound on the correlation between facial skin microbiome and sensitive skin (SS) using a novel sequencing technique.
OBJECTIVE
This study aimed to expound on the correlation between facial skin microbiome and sensitive skin (SS) using a novel sequencing technique.
METHODS
We applied the 2bRAD sequencing for the microbiome, which enables accurate characterization of the low-biomass microbiome at species resolution to profile facial skin microbes in SS and non-SS groups. Further, the bacterial colonies were isolated and cultured from skin surfaces to study the pro-inflammatory effect on human keratinocytes by ELISA.
RESULTS
We accordingly identified 1142 genera and 4436 strains. In the SS group, the proportions of Actinomyces and Microbotryomycetes were significantly increased, whereas that of Acidimicrobiia was decreased. Kruskal-Wallis analysis revealed significant differences in 11 genera and 35 species, among which the proportions of Dermabacter, Chryseobacterium, Rhodotorula and Peptoniphilus A were increased in the SS group. Analysis of the top 10 genera revealed increased proportions of Cutibacterium, Corynebacterium and Staphylococcus. Moreover, the proportion of Dermabacter hominis was significantly increased by 18.9-fold in the SS group, whereas those of many Streptococcus strains were significantly decreased. Focus on the isolated bacterial colonies from skin surfaces, more yellow colonies were found in SS group when cultured in Tryptic Soy Broth medium for 48 h, and more interleukin-8 was detected on keratinocytes after yellow colonies stimulation, such as S.capitis, M.luteus.
CONCLUSIONS
This study suggests that more SS-associated microorganisms can be identified using the 2bRAD technique even with a small sample size. Dermabacter hominis and Chryseobacterium was firstly reported with a significantly increase in SS, and the S.capitis, as well as M.luteus, but not S.aureus, may be associated with skin inflammation.
Topics: Humans; Skin; Microbiota; Face; Adult; Female; Keratinocytes; Middle Aged
PubMed: 38229273
DOI: 10.1111/ics.12941 -
Evidence-based Complementary and... 2017Several species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. In the...
Several species are known for their uses in traditional medicine and consequently are studied for their phytochemical content and their biological activities. In the framework of a previous study conducted on eight extremophile plants from Tunisia, we highlighted that the crude methanolic extract of , a not investigated thistle, showed moderate but quite selective cytotoxic activity against the cancerous cell line J774 compared to the noncancerous cell line WI38 (IC = 11.53 g/ml on J774, IC = 29.89 g/ml on WI38, and selectivity index = 2.6). In the current study, the partitions of the leaves of were analyzed for their antiproliferative activity on the same cell lines. From the most active petroleum ether partition, we isolated four triterpenoids including lupeol, taraxasterol acetate, and a (1 : 1) mixture of 25-hydroperoxycycloart-23-en-3-ol and 24-hydroperoxycycloart-25-en-3-ol. These two cycloartane-type triterpenoids are mostly responsible for this cytotoxic activity. On the other hand, the antimicrobial potential of this plant was also evaluated against 36 microorganisms. The moderate antibacterial activity against 6 and 2 strains is mainly attributed to the butanol partition whose major compounds are glycosides of flavones.
PubMed: 28785293
DOI: 10.1155/2017/7247016 -
Microbial Ecology in Health and Disease 2014Coryneform bacteria constitute an important segment of male urogenital microbiota. They have been generally considered as saprophytes, although some species have been...
BACKGROUND
Coryneform bacteria constitute an important segment of male urogenital microbiota. They have been generally considered as saprophytes, although some species have been associated with prostatitis as well. At the same time, biofilm infections have been suspected as a cause of prostatitis.
OBJECTIVE
To identify a set of coryneform bacteria isolated from semen of either healthy men or prostatitis patients applying different methods to reveal inter-assay variability and to determine their ability of adhesion and biofilm production.
DESIGN
Coryneform bacteria were identified by API Coryne 2.0 biochemical identification system and 16S rDNA sequencing using different primer sets. Quantitative assessment of biofilm production was performed using crystal violet binding assay method.
RESULTS
The most common species were Corynebacterium seminale, C. minutissimum, and Dermabacter hominis. Altogether 14 species and related genera were found. We observed the best inter-assay agreement when identifying C. seminale. Biofilm was observed in 7 out of 24 strains. The biofilm-producing strains belonged to Arthrobacter cumminsii, Dermabacter hominis, C. minutissimum, and Actinomyces neuii. No differences were found between the strains originating from prostatitis patients and healthy men. Dermabacter hominis strains were more potent biofilm producers than C. seminale strains (p=0.048).
CONCLUSIONS
We can conclude that a wide variety of coryneform bacteria can be found from the male genital tract, although their exact identification is problematic due to insufficient representation in databases. Nearly one third of the strains are able to form biofilm that may give them an advantage for surviving several host- and treatment-related conditions.
PubMed: 24563649
DOI: 10.3402/mehd.v25.22701