-
International Journal of Surgical... Sep 2017
Topics: Aged; Carcinoma, Basal Cell; Dermis; Humans; Male; Neoplasm Invasiveness; Skin Neoplasms
PubMed: 28381139
DOI: 10.1177/1066896917696752 -
Wound Repair and Regeneration :... 2003Novel approaches to healing of chronic wounds, such as venous leg ulcers, include the use of tissue-engineered skin substitutes, e.g., human fibroblast-derived dermis....
Novel approaches to healing of chronic wounds, such as venous leg ulcers, include the use of tissue-engineered skin substitutes, e.g., human fibroblast-derived dermis. The exact mechanisms of action of these products and their effects on wound healing at a cellular level are yet to be fully defined. The aim of our study was to evaluate the potential effects of human fibroblast-derived dermis on the healing of chronic wounds using an experimental model. We used a tissue expansion model to examine the effect of human fibroblast-derived dermis on the growth of human tissue biopsied from venous leg ulcers. Further characterization of the cytokine profile produced by human fibroblast-derived dermis in culture was performed using enzyme-linked immunosorbent assay techniques. Addition of medium conditioned with human fibroblast-derived dermis significantly increased the outgrowth of cells from venous leg ulcer biopsies (p = 0.001). We detected bioactive levels of hepatocyte growth factor/scatter factor and interleukin-8 in media conditioned with human fibroblast-derived dermis. Therefore, conditioned media from human fibroblast-derived dermis enhances ex vivo expansion of tissue taken from chronic venous leg ulcers, and contains potent angiogenic factors. These experimental findings may explain the enhanced healing seen with clinical applications of human fibroblast-derived dermis on chronic wounds.
Topics: Cell Culture Techniques; Cell Movement; Dermis; Fibroblasts; Humans; Models, Biological; Skin Transplantation; Time Factors; Tissue Expansion; Varicose Ulcer; Wound Healing
PubMed: 12846917
DOI: 10.1046/j.1524-475x.2003.11409.x -
Medecine Sciences : M/S Apr 2008
Topics: Dendritic Cells; Dermis; Epidermal Cells; Epidermis; Humans; Monocytes; Stem Cells
PubMed: 18405622
DOI: 10.1051/medsci/2008244346 -
Optics Express Jun 2008Dermis is the major source of the fluorescence and light scattering of skin. Tumor-induced degradation of the dermis is expected to change the fluorescence and light...
Dermis is the major source of the fluorescence and light scattering of skin. Tumor-induced degradation of the dermis is expected to change the fluorescence and light scattering properties of skin. To investigate how these fluorescence and light scattering properties are changed, human skin dermis was degraded with enzymes to mimic tumor invasion. The enzymatic erosion process was investigated with fluorescence and reflectance spectroscopy. Dermis degradation by the enzymes resulted in a decrease in fluorescence emission and light scattering in the dermis. Fluorescence anisotropy, however, could not detect the change in the dermis induced by the enzyme treatments.
Topics: Dermis; Dermoscopy; Endopeptidases; Humans; In Vitro Techniques; Microscopy, Fluorescence; Spectrometry, Fluorescence
PubMed: 18575556
DOI: 10.1364/oe.16.009857 -
Journal of Dermatological Science Feb 2018
Topics: Cell Differentiation; Cells, Cultured; Collagen Type V; Dermis; Fibroblasts; Gene Expression Profiling; Humans; Stem Cells
PubMed: 29146132
DOI: 10.1016/j.jdermsci.2017.10.005 -
Clinical and Experimental Dermatology Oct 2011Multipotent cells can be isolated from dermis, and have wound-healing and antioxidant effects on human and murine skin.
BACKGROUND
Multipotent cells can be isolated from dermis, and have wound-healing and antioxidant effects on human and murine skin.
AIM
To investigate the changes in aged dermis after injection of dermal multipotent cells and adult fibroblasts.
METHODS
Dermal multipotent cells were isolated and cultured from 3-day-old BALB/c mice by a regular dermal fibroblast culture system. We used various inducers to confirm the multilineage differentiation of these cells, then the dermal multipotent cells were transplanted into the dermis of aged (12-week-old) BALB/c mice. At 2 and 4 weeks after transplantation, we examined dermal thickness, and determined the amounts of collagen type I present, using PCR and western blotting.
RESULT
The dermal multipotent cells exhibited adipogenic and osteogenic phenotypes when cultured in the presence of certain inducers. Dermal thickness and collagen content in the dermis also increased in the mice injected with dermal multipotent cells, which also had increased RNA and protein expression of collagen type I.
CONCLUSIONS
Collectively, these results indicate that dermal multipotent cells are more effective than fibroblasts in increasing collagen type I and dermal thickness, which may be of importance in preventing skin ageing.
Topics: Animals; Blotting, Western; Collagen Type I; Dermis; Fibroblasts; Mice; Mice, Inbred BALB C; Multipotent Stem Cells; Polymerase Chain Reaction; Skin Aging; Up-Regulation
PubMed: 21507040
DOI: 10.1111/j.1365-2230.2011.04071.x -
ACS Applied Materials & Interfaces Sep 2019Engineering bioscaffolds for improved cutaneous tissue regeneration remains a healthcare challenge because of the increasing number of patients suffering from acute and...
Engineering bioscaffolds for improved cutaneous tissue regeneration remains a healthcare challenge because of the increasing number of patients suffering from acute and chronic wounds. To help address this problem, we propose to utilize alfalfa, an ancient medicinal plant that contains antibacterial/oxygenating chlorophylls and bioactive phytoestrogens, as a building block for regenerative wound dressings. Alfalfa carries genistein, which is a major phytoestrogen known to accelerate skin repair. The scaffolds presented herein were built from composite alfalfa and polycaprolactone (PCL) nanofibers with hydrophilic surface and mechanical stiffness that recapitulate the physiological microenvironments of skin. This composite scaffold was engineered to have aligned nanofibrous architecture to accelerate directional cell migration. As a result, alfalfa-based composite nanofibers were found to enhance the cellular proliferation of dermal fibroblasts and epidermal keratinocytes in vitro. Finally, these nanofibers exhibited reproducible regenerative functionality by promoting re-epithelialization and granulation tissue formation in both mouse and human skin, without requiring additional proteins, growth factors, or cells. Overall, these findings demonstrate the potential of alfalfa-based nanofibers as a regenerative platform toward accelerating cutaneous tissue repair.
Topics: Cell Line; Dermis; Humans; Keratinocytes; Medicago sativa; Nanocomposites; Nanofibers; Polyesters; Wound Healing
PubMed: 31369233
DOI: 10.1021/acsami.9b07626 -
PloS One 2014Dermis isolated adult stem (DIAS) cells, a subpopulation of dermis cells capable of chondrogenic differentiation in the presence of cartilage extracellular matrix, are a...
Dermis isolated adult stem (DIAS) cells, a subpopulation of dermis cells capable of chondrogenic differentiation in the presence of cartilage extracellular matrix, are a promising source of autologous cells for tissue engineering. Hypoxia, through known mechanisms, has profound effects on in vitro chondrogenesis of mesenchymal stem cells and could be used to improve the expansion and differentiation processes for DIAS cells. The objective of this study was to build upon the mechanistic knowledge of hypoxia and translate it to tissue engineering applications to enhance chondrogenic differentiation of DIAS cells through exposure to hypoxic conditions (5% O2) during expansion and/or differentiation. DIAS cells were isolated and expanded in hypoxic (5% O2) or normoxic (20% O2) conditions, then differentiated for 2 weeks in micromass culture on chondroitin sulfate-coated surfaces in both environments. Monolayer cells were examined for proliferation rate and colony forming efficiency. Micromasses were assessed for cellular, biochemical, and histological properties. Differentiation in hypoxic conditions following normoxic expansion increased per cell production of collagen type II 2.3 fold and glycosaminoglycans 1.2 fold relative to continuous normoxic culture (p<0.0001). Groups expanded in hypoxia produced 51% more collagen and 23% more GAGs than those expanded in normoxia (p<0.0001). Hypoxia also limited cell proliferation in monolayer and in 3D culture. Collectively, these data show hypoxic differentiation following normoxic expansion significantly enhances chondrogenic differentiation of DIAS cells, improving the potential utility of these cells for cartilage engineering.
Topics: Adult; Adult Stem Cells; Cartilage; Cell Differentiation; Cell Hypoxia; Cell Proliferation; Chondrogenesis; Collagen; Culture Media; Dermis; Glycosaminoglycans; Humans; Tissue Engineering
PubMed: 24867063
DOI: 10.1371/journal.pone.0098570 -
The International Journal of... 2004Skin morphogenesis occurs following a continuous series of cell-cell interactions which can be subdivided into three main stages: 1- the formation of a dense dermis and... (Review)
Review
Skin morphogenesis occurs following a continuous series of cell-cell interactions which can be subdivided into three main stages: 1- the formation of a dense dermis and its overlying epidermis in the future appendage fields (macropattern); 2- the organization of these primary homogeneous fields into heterogeneous ones by the appearance of cutaneous appendage primordia (micropattern) and 3- cutaneous appendage organogenesis itself. In this review, we will first show, by synthesizing novel and previously published data from our laboratory, how heterogenetic and heterospecific dermal/epidermal recombinations have allowed us to distinguish between the respective roles of the dermis and the epidermis. We will then summarize what is known from the work of many different research groups about the molecular signaling which mediates these interactions in order to introduce the following articles of this Special Issue and to highlight what remains to done.
Topics: Animals; Dermis; Embryonic Induction; Epidermal Cells; Epidermis; Feathers; Models, Biological; Morphogenesis; Mutation; Organ Culture Techniques; Signal Transduction; Skin; Vertebrates
PubMed: 15272376
DOI: 10.1387/ijdb.15272376 -
Journal of Dermatological Science Dec 2000The skin consists of two main layers, epidermis and dermis, separated by the basement membrane. Epidermal-dermal communication through the basement membrane is important... (Review)
Review
The skin consists of two main layers, epidermis and dermis, separated by the basement membrane. Epidermal-dermal communication through the basement membrane is important for skin homeostasis. The basement membrane contains specialized structures, called the anchoring complex, which ensure the stability of connection and communication between these two tissue compartments. The proteins within the anchoring complex provide links to both the intracellular cytoskeletal keratins in keratinocytes and connective tissue proteins of the dermis. One of the key components of the complex is laminin 5, which is essential to epidermal cell attachment. The biological function of laminin 5 has been investigated by using a skin equivalent model in vitro and during keratinocyte sheet grafting in vivo. As a major link between the epidermal basal cells and the papillary dermis, laminin 5 initiates hemidesmosome formation and provides stable attachment of the epidermis to the dermis. Laminin 5 also accelerates the assembly of basement membranes and may enhance the recovery of damaged skin. An intact basement membrane at the epidermal-dermal junction is essential to stability of the skin.
Topics: Animals; Basement Membrane; Child; Dermis; Epidermal Cells; Epidermis; Humans; Laminin; Skin Physiological Phenomena
PubMed: 11137397
DOI: 10.1016/s0923-1811(00)00142-0