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New Biotechnology Dec 2022A range of Desulfovibrio spp. can reduce metal ions to form metallic nanoparticles that remain attached to their surfaces. The bioreduction of palladium (Pd) has been...
A range of Desulfovibrio spp. can reduce metal ions to form metallic nanoparticles that remain attached to their surfaces. The bioreduction of palladium (Pd) has been given considerable attention due to its extensive use in areas of catalysis and electronics and other technological domains. In this study we report, for the first time, evidence for Pd(II) reduction by the highly corrosive Desulfovibrio ferrophilus IS5 strain to form surface attached Pd nanoparticles, as well as rapid formation of Pd(0) coated microbial nanowires. These filaments reached up to 8 µm in length and led to the formation of a tightly bound group of interconnected cells with enhanced ability to attach to a low carbon steel surface. Moreover, when supplied with high concentrations of Pd (≥ 100 mmol Pd(II) g dry cells), both Desulfovibrio desulfuricans and D. ferrophilus IS5 formed bacteria/Pd hybrid porous microstructures comprising millions of cells. These three-dimensional structures reached up to 3 mm in diameter with a dose of 1200 mmol Pd(II) g dry cells. Under suitable hydrodynamic conditions during reduction, two-dimensional nanosheets of Pd metal were formed that were up to several cm in length. Lower dosing of Pd(II) for promoting rapid synthesis of metal coated nanowires and enhanced attachment of cells onto metal surfaces could improve the efficiency of various biotechnological applications such as microbial fuel cells. Formation of biologically stimulated Pd microstructures could lead to a novel way to produce metal scaffolds or nanosheets for a wide variety of applications.
Topics: Palladium; Desulfovibrio desulfuricans; Desulfovibrio; Catalysis
PubMed: 36396027
DOI: 10.1016/j.nbt.2022.11.001 -
Biofouling Mar 2023Microbial biofilms of sulfate-reducing bacteria SRB1 and SRB2 were evaluated on polyethylene terephthalate in mono- and associative bacterial cultures. strains C1 and...
Microbial biofilms of sulfate-reducing bacteria SRB1 and SRB2 were evaluated on polyethylene terephthalate in mono- and associative bacterial cultures. strains C1 and C2b suppressed both the formation of biofilm and reduced the number of sulfate-reducing bacteria in the biofilm on the polyethylene terephthalate during the 50-day experiment. A decrease in the number of sulfate-reducing bacteria compared to the monoculture was also noted in association of SRB1 Sat1 (bacterium-satellite of the sulfate-reducing bacteria). The strain Sat1 was identified as based on some microbiological, physiological and biochemical, genetic features. The importance of studying existing interactions between microorganisms in the ferrosphere and plastisphere is emphasized.
Topics: Polyethylene Terephthalates; Biofilms; Bacteria; Desulfovibrio; Sulfates
PubMed: 37222310
DOI: 10.1080/08927014.2023.2215694 -
Bacteriological Reviews Dec 1965
Review
Topics: Bacteria; Desulfovibrio; In Vitro Techniques
PubMed: 5322044
DOI: 10.1128/br.29.4.425-441.1965 -
Scientific Reports Sep 2018The intestinal microbiota plays a key role in the maintenance of human health. Alterations in this microbiota have been described in several autoimmune diseases,...
The intestinal microbiota plays a key role in the maintenance of human health. Alterations in this microbiota have been described in several autoimmune diseases, including nervous system diseases. Nevertheless, the information regarding neuromuscular conditions is still limited. In this study, we aimed at characterizing the intestinal microbiota composition in myasthenia gravis patients (MG). To this end fecal samples were taken from ten patients, with antibodies against the acetylcholine receptor, and ten age and sex matched controls from the same population (Asturias region, Spain). Fecal samples were submitted to microbiota analyses by 16S rRNA gene profiling, bifidobacterial ITS-region profiling and qPCR. The fecal levels of short chain fatty acids were determined by gas chromatography. MG patients were found to harbor lower relative proportions of Verrucomicrobiaceae and Bifidobacteriaceae, among others, and increased of the phylum Bacteroidetes and the family Desulfovibrionaceae. The increase of these latter microbial groups was also confirmed at quantitative level by qPCR. In contrast, no statistically significant differences were found between MG patients and the control group in the bifidobacterial population at the species level or in short chain fatty acids profiles. Our data indicates an altered fecal microbiota pattern in MG patients and point out at specific microbiota targets for intervention in this population.
Topics: Aged; Aged, 80 and over; Bacteroidetes; Bifidobacterium; Desulfovibrionaceae; Feces; Female; Gastrointestinal Microbiome; Humans; Male; Middle Aged; Myasthenia Gravis; RNA, Ribosomal, 16S; Transcriptome; Verrucomicrobia
PubMed: 30258104
DOI: 10.1038/s41598-018-32700-y -
Water Research Feb 2018The intensive use of silver nanoparticles (AgNPs) in cosmetics and textiles causes their release into sewer networks of urban water systems. Although a few studies have...
The intensive use of silver nanoparticles (AgNPs) in cosmetics and textiles causes their release into sewer networks of urban water systems. Although a few studies have investigated antimicrobial activities of nanoparticles against environmental bacteria, little is known about potential impacts of the released AgNPs on sulfate reducing bacteria in sewers. Here, we investigated the effect of AgNPs on Desulfovibrio vulgaris Hidenborough (D. vulgaris), a typical sulfate-reducing bacterium (SRB) in sewer systems. We found AgNPs stimulated the proliferation of D. vulgaris, rather than exerting inhibitory or biocidal effects. Based on flow cytometer detections, both the cell growth rate and the viable cell ratio of D. vulgaris increased during exposure to AgNPs at concentrations of up to 100 mg/L. The growth stimulation was dependent on the AgNP concentration. These results imply that the presence of AgNPs in sewage may affect SRB abundance in sewer networks. Our findings also shed new lights on the interactions of nanoparticles and bacteria.
Topics: Bacteria; Cell Proliferation; Desulfovibrio vulgaris; Lactates; Metal Nanoparticles; Sewage; Silver; Sulfates
PubMed: 29149671
DOI: 10.1016/j.watres.2017.11.021 -
Journal of Infection and Chemotherapy :... Oct 2008Desulfovibrio species are anaerobic gram-negative, pleomorphic bacilli rarely causing infection in humans. In the present report, we describe a case of bacteremia caused...
Desulfovibrio species are anaerobic gram-negative, pleomorphic bacilli rarely causing infection in humans. In the present report, we describe a case of bacteremia caused by Desulfovibrio fairfieldensis. The patient, for whom biapenem was administered, rapidly improved without any sequelae. As far as we know, this is the first case report of infection by Desulfovibrio species in Japan.
Topics: Aged; Anti-Bacterial Agents; Bacteremia; Cefazolin; Desulfovibrio; Desulfovibrionaceae Infections; Female; Humans; Microbial Sensitivity Tests; RNA, Bacterial; RNA, Ribosomal, 16S; Thienamycins
PubMed: 18936890
DOI: 10.1007/s10156-008-0629-9 -
Applied Microbiology and Biotechnology Jul 2017Trihalomethanes such as chloroform and bromoform, although well-known as a prominent class of disinfection by-products, are ubiquitously distributed in the environment...
Trihalomethanes such as chloroform and bromoform, although well-known as a prominent class of disinfection by-products, are ubiquitously distributed in the environment due to widespread industrial usage in the past decades. Chloroform and bromoform are particularly concerning, of high concentrations detected and with long half-lives up to several hundred days in soils and groundwater. In this study, we report a Dehalobacter- and Desulfovibrio-containing co-culture that exhibits dehalogenation of chloroform (~0.61 mM) to dichloromethane and bromoform (~0.67 mM) to dibromomethane within 10-15 days. This co-culture was further found to dechlorinate 1,1,1-trichloroethane (1,1,1-TCA) (~0.65 mM) to 1,1-dichloroethane within 12 days. The Dehalobacter species present in this co-culture, designated Dehalobacter sp. THM1, was found to couple growth with dehalogenation of chloroform, bromoform, and 1,1,1-TCA. Strain THM1 harbors a newly identified reductive dehalogenase (RDase), ThmA, which catalyzes chloroform, bromoform, and 1,1,1-TCA dehalogenation. Additionally, based on the sequences of thmA and other identified chloroform RDase genes, ctrA, cfrA, and tmrA, a pair of chloroform RDase gene-specific primers were designed and successfully applied to investigate the chloroform dechlorinating potential of microbial communities. The comparative analysis of chloroform RDases with tetrachloroethene RDases suggests a possible approach in predicting the substrate specificity of uncharacterized RDases in the future.
Topics: Catalysis; Coculture Techniques; Desulfovibrionaceae; Ethyl Chloride; Halogenation; Oxidoreductases; Peptococcaceae; Substrate Specificity; Trihalomethanes
PubMed: 28424844
DOI: 10.1007/s00253-017-8236-2 -
Biotechnology and Bioengineering Jun 2018In situ bioreduction of soluble hexavalent uranium U(VI) to insoluble U(IV) (as UO ) has been proposed as a means of preventing U migration in the groundwater. This work...
In situ bioreduction of soluble hexavalent uranium U(VI) to insoluble U(IV) (as UO ) has been proposed as a means of preventing U migration in the groundwater. This work focuses on the bioreduction of U(VI) and precipitation of U(IV). It uses anaerobic batch reactors with Desulfovibrio vulgaris, a well-known sulfate, iron, and U(VI) reducer, growing on lactate as the electron donor, in the absence of sulfate, and with a 30-mM bicarbonate buffering. In the absence of sulfate, D. vulgaris reduced >90% of the total soluble U(VI) (1 mM) to form U(IV) solids that were characterized by X-ray diffraction and confirmed to be nano-crystalline uraninite with crystallite size 2.8 ± 0.2 nm. pH values between 6 and 10 had minimal impact on bacterial growth and end-product distribution, supporting that the mono-nuclear, and poly-nuclear forms of U(VI) were equally bioavailable as electron acceptors. Electron balances support that H transiently accumulated, but was ultimately oxidized via U(VI) respiration. Thus, D. vulgaris utilized H as the electron carrier to drive respiration of U(VI). Rapid lactate utilization and biomass growth occurred only when U(VI) respiration began to draw down the sink of H and relieve thermodynamic inhibition of fermentation.
Topics: Bioreactors; Biotransformation; Culture Media; Desulfovibrio vulgaris; Hydrogen; Hydrogen-Ion Concentration; Lactates; Oxidation-Reduction; Uranium
PubMed: 29476629
DOI: 10.1002/bit.26579 -
Journal of Hazardous Materials Oct 2021Uranium in groundwater during uranium mining activities urgently needs to be remediated through effective and environmental-friendly approaches. The reduction and...
Uranium in groundwater during uranium mining activities urgently needs to be remediated through effective and environmental-friendly approaches. The reduction and immobilization of soluble U(VI) using biogenic carboxymethyl cellulose modified iron sulfide complex (biogenic CMC-FeS complex) is one of the emerging and innovative methods. However, its removal mechanism is largely unknown. Here, biogenic CMC-FeS complex with extracellular polymeric substances (EPS) and CMC was successfully synthesized by sulfate-reducing bacteria (SRB) and showed highly dispersible capacity. The tryptophan and tyrosine, which were the main components in EPS produced by SRB on CMC-FeS surface, significantly increased the U(VI) removal capacity of the biogenic CMC-FeS complex compared with chemically synthesized CMC-FeS. U(VI) removal was attributed to the adsorption of soluble U(VI) by ≡FeO, CMC, tryptophan, and tyrosine on the biogenic CMC-FeS complex, following its reduction by S, S and Fe. Moreover, biogenic CMC-FeS complex with CMC-to-FeS molar ratio of 0.0005 performed well in the presence of bicarbonate (5 mM), humic acid (10 mg/L), or co-existing cations such as Pb, Ni, Cd, Mn and Cu (200 ug/L) at pH 7.0, and displayed relatively high oxidation resistance and stability ability. This work provides an in-depth understanding of the biogenic CMC-FeS complex for the U(VI) removal and contributes to the development of cost-effective U(VI) remediation technologies.
Topics: Adsorption; Desulfovibrio; Groundwater; Oxidation-Reduction; Sulfates; Uranium
PubMed: 34329121
DOI: 10.1016/j.jhazmat.2021.126645 -
Environmental Microbiology Nov 2020Direct electron uptake is emerging as a key process for electron transfer in anaerobic microbial communities, both between species and from extracellular sources, such...
Direct electron uptake is emerging as a key process for electron transfer in anaerobic microbial communities, both between species and from extracellular sources, such as zero-valent iron (Fe ) or cathodic surfaces. In this study, we investigated cathodic electron uptake by Fe -corroding Desulfovibrio ferrophilus IS5 and showed that electron uptake is dependent on direct cell contact via a biofilm on the cathode surface rather than through secreted intermediates. Induction of cathodic electron uptake by lactate-starved D. ferrophilus IS5 cells resulted in the expression of all components necessary for electron uptake; however, protein synthesis was required for full biofilm formation. Notably, proteinase K treatment uncoupled electron uptake from biofilm formation, likely through proteolytic degradation of proteinaceous components of the electron uptake machinery. We also showed that cathodic electron uptake is dependent on SO reduction. The insensitivity of Fe corrosion to proteinase K treatment suggests that electron uptake from a cathode might involve different mechanism(s) than those involved in Fe corrosion.
Topics: Bacterial Proteins; Biofilms; Biological Transport; Corrosion; Desulfovibrio; Electrodes; Electrons; Iron; Oxidation-Reduction; Sulfates
PubMed: 32939950
DOI: 10.1111/1462-2920.15235