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Journal of Hazardous Materials Oct 2023In this study, we developed a colorimetric ozone passive sampler (OPS) incorporating o-dianisidine, a redox dye, into a polydimethylsiloxane sheet. The reaction between...
In this study, we developed a colorimetric ozone passive sampler (OPS) incorporating o-dianisidine, a redox dye, into a polydimethylsiloxane sheet. The reaction between ozone (O) and o-dianisidine result in a visible yellowish color change. Unlike previous passive methods that rely on nitrate extraction or the color disappearance of indigotrisulfonate, the OPS offered improved recognition of average O exposure. To optimize OPS based on time-weighted average (TWA), we extracted and quantified the amount of reacted o-dianisidine after exposing OPS to O by varying concentrations (0-200 ppb) within 8 h. Colorimetric changes of OPS were further analyzed by capturing images, and the effective absorbance of blue scale showed the best fit (EA, R =0.997). OPS validation on visual detection assessed by six parameters: limit of detection, limit of quantification, reproducibility, sampling rate, selectivity to interfering gases, and sensitivity to environmental factors. To enhance visibility, the OPS was assembled with coloration exposure guidelines, and a smartphone app was developed to quantify average O exposures. We further conducted field tests that showed the significant disparity between O concentrations and personal O exposures, which is considered more crucial for assessing health risks. The OPS was optimized to monitor O exposure levels and raise awareness among workers and occupants regarding invisible indoor hazards.
Topics: Humans; Colorimetry; Dianisidine; Reproducibility of Results; Levonorgestrel; Ozone
PubMed: 37703734
DOI: 10.1016/j.jhazmat.2023.132510 -
Bioelectrochemistry (Amsterdam,... Aug 2004o-Dianisidine (3,3'-dimethoxybenzidine) is applied in the production of some dyes and also used in analytical tests. However, this compound is anticipated to be a human...
o-Dianisidine (3,3'-dimethoxybenzidine) is applied in the production of some dyes and also used in analytical tests. However, this compound is anticipated to be a human carcinogen. An analytical strategy utilizing square wave voltammetry for the determination of o-dianisidine is presented. An electrochemical system was consisted of three electrodes: carbon paste working electrode, platinum wire counter electrode and silver-silver chloride (Ag/AgCl) reference electrode. However, square wave voltammograms of direct measurements of o-dianisidine were found to be hardly reproducible, exhibiting few peaks due to some labile short-lived intermediates with the only exception of a quite stable peak at +0.7 V vs. Ag/AgCl. Quantitative determination of o-dianisidine gave satisfactory results only when the carbon paste working electrode was replaced by deoxyribonucleic acids (DNA) electrode obtained by immobilization of double-stranded (ds) DNA on carbon electrode. Square wave voltammogram of DNA showed two peaks attributed to adenine and guanine and the latter was used as analytical signal. After interaction with o-dianisidine, guanine oxidation peak was reduced to the extent related to the concentration of the analyte. Initial reduction of guanine peak took place already at the concentration of o-dianisidine equal to 0.4 microM; high concentrations (above 100 microM) of the analyte quenched completely a guanine response. The presented electrochemical system enables a specific detection of o-dianisidine by the presence of an oxidation peak at +0.7 V and its quantitative determination by measuring a reduction of guanine peak by means of a DNA sensor.
Topics: Adenine; Animals; Biosensing Techniques; Calibration; Carbon; Coloring Agents; DNA; Dianisidine; Electrochemistry; Electrodes; Guanine; Humans; Oxidation-Reduction; Potentiometry; Silver; Silver Compounds
PubMed: 15219251
DOI: 10.1016/j.bioelechem.2004.03.004 -
Agents and Actions Mar 1977Until now o-dianisidine was used as an indicator substance in a test system for the determination of diamine oxidase. More recently, however, this substance was also... (Comparative Study)
Comparative Study
Until now o-dianisidine was used as an indicator substance in a test system for the determination of diamine oxidase. More recently, however, this substance was also used to measure ceruloplasmin activity. A study of the test principles revealed that o-dianisidine was the one denominator for both enzymes. As it was found for diamine oxidase the indicator was oxidized via peroxidase mediated H2O2 cleavage. Ceruloplasmin, however, oxidized o-dianisidine directly with resulting free radical formation. An addition of histamine dihydrochloride or putrescine dihydrochloride to an incubation mixture, containing ceruloplasmin as enzyme and o-dianisidine or p-phenylene-diamine as substrates, produced an activation of the enzyme, being more than 10-fold in the presence of 1 X 10(-2) M putrescine at pH 7.0. It was assumed that an allosteric effect of the dihydrochloride component might be responsible for this activation. When the activity of purified diamine oxidase was determined by the o-dianisidine test and by the isotope assay, a very good correlation between both methods was found. But, in a mixture of diamine oxidase and ceruloplasmin, no differentiation between the two enzymic activities by the o-dianisidine test was possible. This observation demonstrated an interference of ceruloplasmin when the o-dianisidine method was used for the determination of diamine oxidase activity. To apply our findings also in vivo the amine oxidase activity increasing in guinea-pig plasma during inflammation, was determined by the o-dianisidine test and by specific methods for some amine oxidase. Despite an enhanced oxidation of the o-dianisidine observed, only an increase of ceruloplasmin activity was found. It was concluded that ceruloplasmin had no 'histaminase activity' as has been assumed by other authors using the o-dianisidine test.
Topics: Amine Oxidase (Copper-Containing); Animals; Benzidines; Benzylamine Oxidase; Ceruloplasmin; Dianisidine; Female; Guinea Pigs; Indicators and Reagents; Inflammation; Kinetics; Male; Methods; Peroxidases; Putrescine; Spectrophotometry, Ultraviolet
PubMed: 404858
DOI: 10.1007/BF01964886 -
The Analyst Nov 2001A flow injection analysis (FIA) procedure for the determination of free chlorine in industrial formulations and water samples is proposed. The manifold is provided with...
A flow injection analysis (FIA) procedure for the determination of free chlorine in industrial formulations and water samples is proposed. The manifold is provided with a gas-diffusion unit which permits the removal of interfering species and also the preconcentration of chlorine. The determination of chlorine is performed on the basis of the oxidation by o-dianisidine as a chromogenic reagent to a coloured product which can be monitored at 445 nm. The method (for a preconcentration step of 60 s) is linear over the range 0.04-1.00 mg l(-1) of chlorine, the limit of detection is 0.04 mg l(-1), the reproducibility of the procedure (as RSD of the slope) is 3.7% for a series of four independent calibrations, the precision (as RSD of a series of 30 continuous FIA peaks of 0.56 mg l(-1) of chlorine) is 1.4% and the sample throughput is 40 h(-1). A detailed comparative study of the analytical characteristics of a single mono-channel reverse FIA assembly and the same system but provided with a Fluoropore membrane filter of 0.5 microm pore size was performed to check the advantages of the new approach in terms of sensitivity, selectivity and limit of detection.
Topics: Chlorine; Chromogenic Compounds; Dianisidine; Flow Injection Analysis; Spectrophotometry; Water Pollutants, Chemical
PubMed: 11763097
DOI: 10.1039/b107000m -
The Japanese Journal of Physiology 1981A new method of horseradish peroxidase (HRP) neurohistochemistry using o-dianisidine (OD) as the chromogen was described. In labelling neurones of the hypoglossal... (Comparative Study)
Comparative Study
A new method of horseradish peroxidase (HRP) neurohistochemistry using o-dianisidine (OD) as the chromogen was described. In labelling neurones of the hypoglossal nucleus in rats and cats, the new OD method was as sensitive as Mesulam's method using tetramethyl benzidine (TMB) as the chromogen.
Topics: Animals; Anterior Horn Cells; Benzidines; Brain Stem; Cats; Dianisidine; Female; Histocytochemistry; Horseradish Peroxidase; Male; Methods; Peroxidases; Rats
PubMed: 7289229
DOI: 10.2170/jjphysiol.31.269 -
Journal of Neural Transmission (Vienna,... Oct 2014Ceruloplasmin (Cp) is a serum ferroxidase that plays an essential role in iron metabolism. It is routinely tested by immunoturbidimetric assays that quantify the...
Ceruloplasmin (Cp) is a serum ferroxidase that plays an essential role in iron metabolism. It is routinely tested by immunoturbidimetric assays that quantify the concentration of the protein both in its active and inactive forms. Cp activity is generally analyzed manually; the process is time-consuming, has a limited repeatability, and is not suitable for a clinical setting. To overcome these inconveniences, we have set the automation of the o-dianisidine Cp activity assay on a Cobas Mira Plus apparatus. The automation was rapid and repeatable, and the data were provided in terms of IU/L. The assay was adapted for human sera and showed a good precision [coefficient of variation (CV) 3.7 %] and low limit of detection (LoD 11.58 IU/L). The simultaneous analysis of Cp concentration and activity in the same run allowed us to calculate the Cp-specific activity that provides a better index of the overall Cp status. To test the usefulness of this automation, we tested this assay on 104 healthy volunteers and 36 patients with Wilson's disease, hepatic encephalopathy, and chronic liver disease. Cp activity and specific activity distinguished better patients between groups with respect to Cp concentration alone, and providing support for the clinical investigation of neurological diseases in which liver failure is one of the clinical hallmarks.
Topics: Automation, Laboratory; Blood Chemical Analysis; Ceruloplasmin; Dianisidine; End Stage Liver Disease; Fasting; Hepatic Encephalopathy; Hepatolenticular Degeneration; Humans
PubMed: 24663495
DOI: 10.1007/s00702-014-1196-0 -
Use of glucose oxidase, peroxidase, and O-dianisidine in determination of blood and urinary glucose.Lancet (London, England) Aug 1957
Topics: Blood Glucose; Coloring Agents; Dianisidine; Glucose; Glucose Oxidase; Glycosuria; Humans; Peroxidase; Peroxidases
PubMed: 13464070
DOI: 10.1016/s0140-6736(57)92595-3 -
Clinica Chimica Acta; International... Jun 1976Automated procedures for the kinetic assay of serum ceruloplasmin activity using a bichromatic and a centrifugal analyzer are described. The method is based on the... (Comparative Study)
Comparative Study
Automated procedures for the kinetic assay of serum ceruloplasmin activity using a bichromatic and a centrifugal analyzer are described. The method is based on the oxidase activity of ceruloplasmin at pH 5.0 with o-dianisidine as substrate. Enzyme activity is reported in I.U./l, based on the molar absorption coefficient of o-dianisidine consumed. The substrate is stable and is not subject to non-enzymatic oxidation. Comparison with a manual reference end-point assay using the same substrate indicates good correlation of the bichromatic and centrifugal methods. The analytical precision is comparable to the manual assay for both methods.
Topics: Autoanalysis; Ceruloplasmin; Dianisidine; Evaluation Studies as Topic; Humans; Indicators and Reagents; Kinetics; Methods; Oxidation-Reduction; Spectrophotometry
PubMed: 1277556
DOI: 10.1016/0009-8981(76)90501-5 -
British Medical Journal Aug 1967
Topics: Biochemical Phenomena; Biochemistry; Biphenyl Compounds; Carcinogens; Humans; Occupational Diseases
PubMed: 6038325
DOI: 10.1136/bmj.3.5564.557-d -
Journal of Biochemical and Biophysical... Aug 1979The differential mobilities of compounds in an electric field are important analytical criteria and we can use them to bring electrophoretically pure components of a...
The differential mobilities of compounds in an electric field are important analytical criteria and we can use them to bring electrophoretically pure components of a mixture medium on which they are separated. To this end, the compound undergoing reaction are brought into positions on the carrier to assure optimal contact between selected fractions, within a predetermined domain of time and distance. The appearance of a product defines their reactivities, and the product's continued migration on the same carrier can provide the first key to its identity as is demonstrated and discussed. The method is called reaction electrophoresis and it will be of particular use in studies with labile components. It is illustrated here with the coupling reaction of the sodium salt of 1,4-naphthol sulfonic acid and tetrazotized o-dianisidine.
Topics: Azo Compounds; Diazonium Compounds; Electrophoresis, Polyacrylamide Gel; Naphthalenesulfonates; Spectrophotometry, Ultraviolet
PubMed: 552387
DOI: 10.1016/0165-022x(79)90007-1