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Meditsinskaia Parazitologiia I... 1964
Topics: Anthelmintics; Dichlorophen; Mice; Niclosamide; Pharmacology; Phenols; Research; Toxicology
PubMed: 14331782
DOI: No ID Found -
Journal of Applied Toxicology : JAT 1998As part of a health-hazard survey on the health risk of hospital cleaning workers from exposure to Lyorthol, a hazard assessment of o-benzo-p-chlorophenol, one of the...
As part of a health-hazard survey on the health risk of hospital cleaning workers from exposure to Lyorthol, a hazard assessment of o-benzo-p-chlorophenol, one of the constituents of Lyorthol, has been prepared. In this paper, the physical and chemical characteristics, kinetics and effects of o-benzochlorophenol are described and discussed, and an overall, summarizing hazard evaluation is presented.
Topics: Administration, Oral; Adult; Animals; Dichlorophen; Disinfectants; Female; Housekeeping, Hospital; Humans; Male; Middle Aged; Mutagenicity Tests; Occupational Exposure; Personnel, Hospital; Rabbits; Rats; Reproduction; Skin; Tissue Distribution
PubMed: 9719427
DOI: 10.1002/(sici)1099-1263(199807/08)18:4<271::aid-jat504>3.0.co;2-b -
Acta Odontologica Latinoamericana : AOL 2008The aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the Direct Contact Test. The sealers tested...
The aim of this study was to evaluate in vitro the duration of the antimicrobial effect of endodontic sealers by means of the Direct Contact Test. The sealers tested were: Endomethasone - Septodont, Endomethasone C-Septodont, Endion-Voco, Diaket-ESPE, Pulp Canal Sealer-SybronEndo, and AH26-Dentsply DeTrey. The endodontopathic microorganisms (MO) confronted were: Staphylococcus aureus (Sa), Candida albicans (Ca), Enterococcus faecalis (Ef), Prevotella intermedia (Pi), Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn). Test specimens of each sealer were prepared and placed on the surface of agar plates that had been inoculated with each MO, and after predetermined periods, transfers were made from the contact area between the test specimen and the cultured agar and from the area that had not been in contact with the test specimens (control). The results were read as presence/absence of microbial growth and analyzed statistically using the Kruskal-Wallis test. It was concluded that the structural features and virulence of endodontopathic microorganisms determine their response to the sealers, independently of the time during which sealers act and the mechanism by which the antiseptic reaches the microorganism, which in this case was by direct contact.
Topics: Anti-Infective Agents; Bismuth; Candida albicans; Colony Count, Microbial; Creosote; Dental Pulp Diseases; Dexamethasone; Drug Combinations; Enterococcus faecalis; Epoxy Resins; Formaldehyde; Formocresols; Fusobacterium nucleatum; Glass Ionomer Cements; Humans; Hydrocarbons, Iodinated; Hydrocortisone; Microbiological Techniques; Polyvinyls; Porphyromonas gingivalis; Prevotella intermedia; Root Canal Filling Materials; Silver; Staphylococcus aureus; Thymol; Time Factors; Titanium; Virulence; Zinc Oxide
PubMed: 19177855
DOI: No ID Found -
International Endodontic Journal Mar 2009To evaluate the effect of leakage on differences in genotoxicity of root canal sealers ex vivo according to their main components using two different cytogenetic assays.
AIM
To evaluate the effect of leakage on differences in genotoxicity of root canal sealers ex vivo according to their main components using two different cytogenetic assays.
METHODOLOGY
Six materials of different composition (GuttaFlow, Epiphany, Diaket, IRM, SuperEBA and Hermetic) were tested on human peripheral blood lymphocytes using the comet assay and chromosomal aberration analysis. Prepared materials were eluted in physiological solution for 1 h, 1 day, 5 and 30 days. Thereafter cultures were treated with 8 microg, 4 microg and 2 microg of each sealer. Frequencies of chromatide and chromosome breaks and accentric fragments were determined. Comet assay was used to evaluate primary DNA damage by measuring tail length and tail intensity. Chi-square, Fisher's PLSD (Protected Least Significant Difference) and Kruskall-Wallis non parametric tests were used for statistical analysis.
RESULTS
After 1-h elution only the highest dose of Diaket, Hermetic and SuperEBA significantly (P = 0.035, P = 0.048, P = 0.037 respectively) affected the measured cytogenetic parameters. The migration ability of DNA was more strongly affected than induction of chromosomal aberrations. After elutions longer than 24 h none of the tested sealers exhibited a genotoxic effect.
CONCLUSION
Under the conditions used in the study all sealers had acceptable biocompatibility in terms of genotoxicity.
Topics: Biocompatible Materials; Bismuth; Cell Survival; Cells, Cultured; Chromatids; Chromosome Aberrations; Coloring Agents; Comet Assay; Dental Leakage; Dentin-Bonding Agents; Dimethylpolysiloxanes; Drug Combinations; Gutta-Percha; Humans; Lymphocytes; Materials Testing; Methylmethacrylates; Mutagens; Polyvinyls; Root Canal Filling Materials; Time Factors; Trypan Blue; Zinc Oxide; Zinc Oxide-Eugenol Cement
PubMed: 19228216
DOI: 10.1111/j.1365-2591.2008.01510.x -
Meditsinskaia Parazitologiia I... 1975
Comparative Study
Topics: Alanine Transaminase; Animals; Anticestodal Agents; Ascorbic Acid; Aspartate Aminotransferases; Bile; Bilirubin; Blood Proteins; Calcium; Cholesterol; Cholic Acids; Cholinesterases; Dichlorophen; Glycogen; Guinea Pigs; Liver; Male; Phenols; Rabbits; Rats; Urea
PubMed: 1177836
DOI: No ID Found -
Chemical Biology & Drug Design Aug 2019The need for new antibacterial agents is increasingly becoming of great importance as bacterial resistance to current drugs is quickly spreading. Enoyl-acyl carrier...
The need for new antibacterial agents is increasingly becoming of great importance as bacterial resistance to current drugs is quickly spreading. Enoyl-acyl carrier protein reductases (FabI) are important enzymes for fatty acid biosynthesis in bacteria and other micro-organisms. In this project, we conducted structure-based virtual screening against the FabI enzyme, and accordingly, 37 compounds were selected for experimental testing. Interestingly, five compounds were able to demonstrate antimicrobial effect with variable inhibition activity against various strains of bacteria and fungi. Minimum inhibitory concentrations of the active compounds were determined and showed to be in low to medium micromolar range. Subsequently, enzyme inhibition assay was carried out for our five antimicrobial hits to confirm their biological target and determine their IC values. Three of these tested compounds exhibited inhibition activity for the FabI enzyme where our best hit MN02 had an IC value of 7.8 μM. Furthermore, MN02 is a small bisphenolic compound that is predicted to have all required features to firmly bind with the target enzyme. To sum up, hits discovered in this work can act as a good starting point for the future development of new and potent antimicrobial agents.
Topics: Anti-Bacterial Agents; Bacteria; Bacterial Proteins; Drug Design; Enoyl-(Acyl-Carrier-Protein) Reductase (NADH); Enzyme Inhibitors
PubMed: 31063658
DOI: 10.1111/cbdd.13536 -
Enzyme and Microbial Technology Jan 2013A Structure Activity Relationship (SAR) study for laccase mediator systems was performed in order to correctly classify different natural phenolic mediators. Decision... (Comparative Study)
Comparative Study
A Structure Activity Relationship (SAR) study for laccase mediator systems was performed in order to correctly classify different natural phenolic mediators. Decision tree (DT) classification models with a set of five quantum-chemical calculated molecular descriptors were used. These descriptors included redox potential (ɛ°), ionization energy (E(i)), pK(a), enthalpy of formation of radical (Δ(f)H), and OH bond dissociation energy (D(O-H)). The rationale for selecting these descriptors is derived from the laccase-mediator mechanism. To validate the DT predictions, the kinetic constants of different compounds as laccase substrates, their ability for pesticide transformation as laccase-mediators, and radical stability were experimentally determined using Coriolopsis gallica laccase and the pesticide dichlorophen. The prediction capability of the DT model based on three proposed descriptors showed a complete agreement with the obtained experimental results.
Topics: Acetophenones; Benzaldehydes; Biocatalysis; Catechols; Coumaric Acids; Decision Trees; Dichlorophen; Fungal Proteins; Hydrazones; Laccase; Models, Chemical; Models, Molecular; Molecular Structure; Nitrophenols; Oxidation-Reduction; Phenols; Polyporales; Protein Conformation; Quantitative Structure-Activity Relationship; Vanillic Acid
PubMed: 23199741
DOI: 10.1016/j.enzmictec.2012.10.009 -
Chemistry, An Asian Journal Jul 2022Titanate nanotubes (TNTs) were coated with a cyclic oligosaccharide (carboxymethyl-β-cyclodextrin, CM-β-CD) to obtain a photocatalyst (CM-β-CD-TNT) for efficiently...
Titanate nanotubes (TNTs) were coated with a cyclic oligosaccharide (carboxymethyl-β-cyclodextrin, CM-β-CD) to obtain a photocatalyst (CM-β-CD-TNT) for efficiently activating molecular oxygen and removing the target contaminant. The hydrophobic cavity and the large specific surface area of the photocatalyst provide abundant active sites for activating molecular oxygen. The free radical capture experiment and quenching experiment showed that cyclodextrin could facilitate adsorption and activation of molecular oxygen to produce O . Therefore, compared with the pristine TNT, CM-β-CD-TNT accelerated the oxidation efficiency of paracetamol (APAP) by 3.4 times. Moreover, the ring cleavage reaction induced by CM-β-CD-TNT effectively reduced the acute toxicity of wastewater containing APAP. Furthermore, 100% of bisphenol A (BPA), bisphenol S (BPS), phenol, 2,4-dichlorophen (2,4-DCP), and carbamazepine (CBZ) were degraded by CM-β-CD-TNT after 2.5 h ultraviolet (UV) light irradiation. This strategy provides a new dimension for the advanced treatment of organic wastewater by organic macrocyclic molecule-modified materials.
Topics: Acetaminophen; Nanotubes; Oxygen; Wastewater; beta-Cyclodextrins
PubMed: 35470547
DOI: 10.1002/asia.202200352 -
Pharmaceutical Development and... 2016Chlorophene-loaded nanospheres with various formulation parameters were evaluated. The optimal formulation was found at 0.1% w/v of poloxamer 407, 15 mL of ethyl...
Chlorophene-loaded nanospheres with various formulation parameters were evaluated. The optimal formulation was found at 0.1% w/v of poloxamer 407, 15 mL of ethyl acetate and 20% initial chlorophene loading that provided the suitable size (179 nm), the highest loading content (19.2%), encapsulation efficiency (88.0%) and yield (91.6%). Moreover, encapsulation of chlorophene in nanospheres was able to prolong and sustain drug release over one month. Chlorophene-loaded nanospheres were effective against Staphylococcus aureus (S. aureus) and Candida albicans (C. albicans), the main cause of hospital-acquired infections. Chlorophene-loaded nanospheres were effective against S. aureus (>46 µg/mL) and C. albicans (>184 µg/mL). These nanospheres appeared to have profound effect on the time-dependent hemolytic activity due to gradual release of chlorophene. At the concentration of 46 µg/mL, nearly no HRBC hemolysis in 24 h compared to 80% of hemolysis from free drug. In conclusion, polymeric nanospheres were successfully fabricated to encapsulate chlorophene which can eliminate inherent toxicity of drugs and have potential uses in prolonged release of antimicrobial.
Topics: Anti-Infective Agents; Candida albicans; Chemistry, Pharmaceutical; Delayed-Action Preparations; Dichlorophen; Dose-Response Relationship, Drug; Drug Delivery Systems; Humans; Microbial Sensitivity Tests; Nanospheres; Staphylococcus aureus
PubMed: 25220889
DOI: 10.3109/10837450.2014.959180 -
Ugeskrift For Laeger May 1958
Topics: Animals; Anthelmintics; Cestode Infections; Communicable Diseases; Dichlorophen; Humans; Parasitic Diseases; Quinacrine; Taenia saginata
PubMed: 13569657
DOI: No ID Found