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Lancet (London, England) Jun 1979
Topics: Alopecia; Alopecia Areata; Dinitrochlorobenzene; Humans; Nitrobenzenes
PubMed: 87877
DOI: 10.1016/s0140-6736(79)92052-x -
Annales de Dermatologie Et de... 1984
Review
Topics: Alopecia Areata; Carcinoma, Basal Cell; Dermatitis, Contact; Dinitrochlorobenzene; Humans; Hypersensitivity; Keratosis; Melanoma; Mutation; Neoplasm Metastasis; Nitrobenzenes; Risk; Warts
PubMed: 6393839
DOI: No ID Found -
Archives of Dermatology Mar 1985
Topics: Animals; Carcinogens; Dinitrochlorobenzene; Humans; Mutagenicity Tests; Nitrobenzenes; Rats
PubMed: 3977355
DOI: No ID Found -
Clinical Immunology (Orlando, Fla.) Nov 2022Atopic dermatitis (AD), a type of skin inflammation, is associated with immune response mediated by T-helper 2 (Th2) cells, and mast cells. Vasicine is an alkaloid...
Atopic dermatitis (AD), a type of skin inflammation, is associated with immune response mediated by T-helper 2 (Th2) cells, and mast cells. Vasicine is an alkaloid isolated from Adhatoda vasica, a popular Ayurvedic herbal medicine used for treating inflammatory conditions. In the present study, the anti-AD effects of vasicine were evaluated on 2,4-dinitrochlorobenzene-induced AD-like skin lesions in BALB/c mice. The potential anti-allergic effects of vasicine were also assessed using the passive cutaneous anaphylaxis (PCA) test. The results showed that the oral administration of vasicine improved the severity of AD-like lesional skin by decreasing histopathological changes and restoring epidermal thickness. Vasicine also inhibited the infiltration of mast cells in the skin and reduced the levels of pro-Th2 and Th2 cytokines as well as immunoglobulin E in the serum. Finally, vasicine inhibited the expression of pro-Th2 and Th2 cytokines in skin tissues, indicating the therapeutic potential of vasicine for AD.
Topics: Alkaloids; Animals; Anti-Allergic Agents; Cytokines; Dermatitis, Atopic; Dinitrochlorobenzene; Immunoglobulin E; Mice; Mice, Inbred BALB C; Passive Cutaneous Anaphylaxis; Quinazolines; Skin; Skin Diseases
PubMed: 36049600
DOI: 10.1016/j.clim.2022.109102 -
Journal of the American Academy of... Oct 1983DNCB (2,4-dinitrochlorobenzene) is used in the treatment of alopecia areata, recalcitrant verrucae, and for evaluating immunocompromised patients. Currently, there is no...
DNCB (2,4-dinitrochlorobenzene) is used in the treatment of alopecia areata, recalcitrant verrucae, and for evaluating immunocompromised patients. Currently, there is no pharmaceutical grade of DNCB available for clinical use. Recently, no nitrochlorobenzene contamination was found with the use of positive ion detection gas chromatography--mass spectrometry. We examined six commercial sources of DNCB, with the use of negative ion detection gas chromatography--mass spectrometry, for volatile impurities such as nitrochlorobenzene which might remain from the manufacturing process. The use of negative ion detection increases the sensitivity of this technic to benzene rings substituted with electron withdrawing groups like the nitrochlorobenzenes. We found that all sources of DNCB contain various combinations of nitrochlorobenzene, dinitrobenzene, nitrodichlorobenzene, and other isomers of DNCB. Nitrochlorobenzene has been shown to be mutagenic in the Ames test and carcinogenic in animal studies. The presence of nitrochlorobenzene and other contaminants in commercial grades of DNCB raises questions about the continued clinical application of this agent.
Topics: Chemical Phenomena; Chemistry; Chlorobenzenes; Dinitrobenzenes; Dinitrochlorobenzene; Drug Contamination; Gas Chromatography-Mass Spectrometry; Isomerism; Nitrobenzenes; Volatilization
PubMed: 6630616
DOI: 10.1016/s0190-9622(83)70169-6 -
Archives of Dermatology Aug 1982Forty-two patients with alopecia areata were treated with local applications of dinitrochlorobenzene (DNCB); We used DNCB in two forms, an acetone solution applied... (Clinical Trial)
Clinical Trial
Forty-two patients with alopecia areata were treated with local applications of dinitrochlorobenzene (DNCB); We used DNCB in two forms, an acetone solution applied weekly or a cream used every day, employing a wide range of DNCB concentrations. The concentration used was varied at the time of each application to produce a contact dermatitis. Seven patients experienced complete and lasting hair regrowth, 17 had poor results, and in 18 patients the treatment was a failure. Acquired tolerance to DNCB was observed in six patients; in five it was abolished by the administration of cimetidine. Certain factors such as the delay in appearance and the intensity of the sensitization reaction influence the hair regrowth. Poor prognostic criteria for treatment effect included a history of previous systemic corticosteroid therapy, atopy, and the presence of alopecia areata in close relatives.
Topics: Administration, Topical; Adolescent; Adult; Alopecia Areata; Child; Cimetidine; Dinitrochlorobenzene; Drug Therapy, Combination; Drug Tolerance; Female; Humans; Male; Middle Aged; Nitrobenzenes
PubMed: 7103522
DOI: 10.1001/archderm.118.8.542 -
Archives of Dermatology Nov 1978Ninety patients with alopecia areata were treated with weekly applications to one side of the head of dinitrochlorobenzene (DNCB) dissolved in acetone, the other side of...
Ninety patients with alopecia areata were treated with weekly applications to one side of the head of dinitrochlorobenzene (DNCB) dissolved in acetone, the other side of the head serving as control region. In 80 patients (89%) hair regrew either exclusively on the treated side, or considerably faster and denser on this side. The difference was noted, in the majority of cases, within eight weeks. The initial response, however, could not be maintained in all of these patients. Persistent response was observed in 72 patients (80%). Peribulbar round cell infiltrates were found to be more constant and denser on the treated side, suggesting that topically applied DNCB affects the peribulbar infiltrate present in alopecia areata. Possibly, the therapeutic result is due to altered local immunoregulation.
Topics: Adolescent; Adult; Aged; Alopecia Areata; Child; Dinitrochlorobenzene; Drug Eruptions; Female; Humans; Male; Middle Aged; Nitrobenzenes; Scalp; T-Lymphocytes
PubMed: 152611
DOI: No ID Found -
Mutation Research Nov 1996Dinitrochlorobenzene (DNCB) is clinically efficacious in the therapy of alopecia areata, but its use was limited when it was found to be mutagenic in the Ames test....
Dinitrochlorobenzene (DNCB) is clinically efficacious in the therapy of alopecia areata, but its use was limited when it was found to be mutagenic in the Ames test. However, there has been renewed interest in the immunomodulatory benefits of topically applied dinitrochlorobenzene in patients with human immunodeficiency virus and systemic lupus erythematosus. The current study examines the genotoxicity of dinitrochlorobenzene in human skin fibroblasts using sister chromatid exchange. Dinitrochlorobenzene caused a significant increase in sister chromatid exchange at concentrations ranging from 2.5 to 10 microM. Thus, dinitrochlorobenzene is genotoxic in human skin fibroblasts at concentrations well below those used clinically. The potential for long-term toxicity from dinitrochlorobenzene will have to be weighed against the severity and prognosis of the diseases for which it is used.
Topics: Dinitrochlorobenzene; Fibroblasts; Humans; Mutagens; Sister Chromatid Exchange; Skin; Th1 Cells
PubMed: 8950355
DOI: 10.1016/s0165-1218(96)90099-3 -
Lancet (London, England) Oct 1995
Topics: Administration, Topical; Dinitrochlorobenzene; Humans; Lupus Erythematosus, Systemic; Patch Tests
PubMed: 7564768
DOI: 10.1016/s0140-6736(95)91601-6 -
Zhong Nan Da Xue Xue Bao. Yi Xue Ban =... Jan 2023Ozone is widely applied to treat allergic skin diseases such as eczema, atopic dermatitis, and contact dermatitis. However, the specific mechanism remains unclear. This...
OBJECTIVES
Ozone is widely applied to treat allergic skin diseases such as eczema, atopic dermatitis, and contact dermatitis. However, the specific mechanism remains unclear. This study aims to investigate the effects of ozonated oil on treating 2,4-dinitrochlorobenzene (DNCB)-induced allergic contact dermatitis (ACD) and the underling mechanisms.
METHODS
Besides the blank control (Ctrl) group, all other mice were treated with DNCB to establish an ACD-like mouse model and were randomized into following groups: a model group, a basal oil group, an ozonated oil group, a FcεRI-overexpressed plasmid (FcεRI-OE) group, and a FcεRI empty plasmid (FcεRI-NC) group. The basal oil group and the ozonated oil group were treated with basal oil and ozonated oil, respectively. The FcεRI-OE group and the FcεRI-NC group were intradermally injected 25 µg FcεRI overexpression plasmid and 25 µg FcεRI empty plasmid when treating with ozonated oil, respectively. We recorded skin lesions daily and used reflectance confocal microscope (RCM) to evaluate thickness and inflammatory changes of skin lesions. Hematoxylin-eosin (HE) staining, real-time PCR, RNA-sequencing (RNA-seq), and immunohistochemistry were performed to detct and analyze the skin lesions.
RESULTS
Ozonated oil significantly alleviated DNCB-induced ACD-like dermatitis and reduced the expressions of IFN-γ, IL-17A, IL-1β, TNF-α, and other related inflammatory factors (all P<0.05). RNA-seq analysis revealed that ozonated oil significantly inhibited the activation of the DNCB-induced FcεRI/Syk signaling pathway, confirmed by real-time PCR and immunohistochemistry (all P<0.05). Compared with the ozonated oil group and the FcεRI-NC group, the mRNA expression levels of IFN-γ, IL-17A, IL-1β, IL-6, TNF-α, and other inflammatory genes in the FcεRI-OE group were significantly increased (all P<0.05), and the mRNA and protein expression levels of FcεRI and Syk were significantly elevated in the FcεRI-OE group as well (all P<0.05).
CONCLUSIONS
Ozonated oil significantly improves ACD-like dermatitis and alleviated DNCB-induced ACD-like dermatitis via inhibiting the FcεRI/Syk signaling pathway.
Topics: Animals; Mice; Dinitrochlorobenzene; Skin; Cytokines; Interleukin-17; Tumor Necrosis Factor-alpha; Dermatitis, Allergic Contact; Dermatitis, Atopic; Signal Transduction; RNA, Messenger; Mice, Inbred BALB C
PubMed: 36935172
DOI: 10.11817/j.issn.1672-7347.2023.220082