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Veterinary Parasitology Jun 2002Both Dirofilaria immiti and Dipetalonema reconditum may be found in blood of infected dogs but it is not easy to distinguish D. immitis from D. reconditum in morphology....
Specific polymerase chain reaction for differential diagnosis of Dirofilaria immitis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2).
Both Dirofilaria immiti and Dipetalonema reconditum may be found in blood of infected dogs but it is not easy to distinguish D. immitis from D. reconditum in morphology. We cloned and sequenced the contiguous internal transcribed spacer (ITS) region, ITS1-5.8S-ITS2, of these two different parasites and published on GenBank as AF217800 for D. immiti and AF217801 for D. reconditum in this study. We designed two pairs of specific primers derived from ITS2 being used for polymerase chain reaction (PCR). The amplicons of ITS2 from D. immiti and D. reconditum are 302 and 348bp, respectively. Moreover, the limitation for amplifying ITS2 gene using this PCR demonstrated that 1 x 10(-2) microfilaria of each species of parasite smashed or even with mixed samples could be detected and the PCR products were predicted as the same as that described above. Thus, D. immiti and D. reconditum could be differentially diagnosed by this specific PCR. Seventeen clinical cases were evaluated and all of them were correctly identified. In this study, ITS1-5.8S-ITS2 of D. immiti or D. reconditum were the first time sequenced and analyzed. No significant similarity of ITS1 and ITS2 between D. immiti and D. reconditum could be observed.
Topics: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Helminth; DNA, Ribosomal Spacer; Diagnosis, Differential; Dipetalonema; Dipetalonema Infections; Dirofilaria immitis; Dirofilariasis; Dog Diseases; Dogs; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Alignment
PubMed: 12062512
DOI: 10.1016/s0304-4017(02)00032-8 -
Tropical Medicine and Parasitology :... Mar 1988The susceptibility of hosts to filarial infection during pregnancy and lactation was studied using Dipetalonema viteae in Mastomys natalensis as working model. Though no...
The susceptibility of hosts to filarial infection during pregnancy and lactation was studied using Dipetalonema viteae in Mastomys natalensis as working model. Though no difference in prepatency could be observed yet significantly higher density of microfilaraemia than controls was detected in both pregnant and lactating groups. Extended duration of patency was also found in both pregnant and lactating animals. On autopsy, animals exposed at the time of pregnancy or lactation revealed significantly higher recovery of adult worms as compared to normal controls. Thus pregnancy and/or lactation may lead to higher density and duration of microfilaraemia and enhanced establishment of adult worms in female mastomys which are otherwise less susceptible to filarial infection.
Topics: Animals; Dipetalonema; Dipetalonema Infections; Disease Models, Animal; Disease Susceptibility; Female; Filariasis; Lactation; Muridae; Pregnancy; Pregnancy Complications, Infectious
PubMed: 3387824
DOI: No ID Found -
The American Journal of Tropical... Nov 1978Thirty-three patients in Zaire with streptocerciasis were treated daily with diethylcarbamazine (DEC) for 21 days. Histopathologic studies of biopsy specimens with...
Thirty-three patients in Zaire with streptocerciasis were treated daily with diethylcarbamazine (DEC) for 21 days. Histopathologic studies of biopsy specimens with papules of skin established that during DEC treatment adult male and female Dipetalonema streptocerca die and degenerate. DEC may thus produce radical cures of streptocerciasis.
Topics: Adolescent; Adult; Child; Diethylcarbamazine; Dipetalonema; Dipetalonema Infections; Female; Filariasis; Humans; Male; Middle Aged; Skin
PubMed: 569443
DOI: 10.4269/ajtmh.1978.27.1137 -
The American Journal of Tropical... Nov 1977In 75 biopsy specimens of skin from 34 patients with streptocerciasis who had been treated with diethylcarbamazine, we found 39 female and six male adult Dipetalonema...
In 75 biopsy specimens of skin from 34 patients with streptocerciasis who had been treated with diethylcarbamazine, we found 39 female and six male adult Dipetalonema streptocerca in the dermal collagen. This is the first report of adult male D. streptocerca in man, and identifying features are described.
Topics: Animals; Dipetalonema; Dipetalonema Infections; Female; Filariasis; Humans; Male; Skin
PubMed: 563682
DOI: 10.4269/ajtmh.1977.26.1153 -
Tropenmedizin Und Parasitologie Mar 1979Experimental infections were carried out with the tissue-dwelling filaria Dipetalonema viteae using the argasid tick Ornithodorus moubata as the intermediate and the...
Experimental infections were carried out with the tissue-dwelling filaria Dipetalonema viteae using the argasid tick Ornithodorus moubata as the intermediate and the multimammate rat Mastomys natalensis (Strain GRA Giessen) as the final host. The optimum infective dose was found to be 50 third-stage larvae, which produced patent infections and the recovery rates of adult parasites were 47.6 and 26.4% of the inoculated larvae 140 and 189 days after infection, respectively. After an average prepatent period of 57 days, the microfilaraemia increased progressively and reached relatively low maximum values about 192 days after infection. These maximum values were followed by rapid decrease of microfilaraemia, but microfilariae were still detectable at 261 days post infection. Following the subcutaneous injection of infected animals with dexamethasone in single doses each of 1, 10 or 20 mg/kg body weight 30 minutes before blood puncture, a dose-dependent increase in the microfilarial counts in the circulating blood was observed, this reaching maximum values between 120 and 160 days after infection. Repeated administration of single doses of 10 mg/kg dexamethasone revealed an uniform but temporary increase in the microfilaraemia but this was not associated with any alterations in the reproductive organs of adult female parasites. No correlation could be found between the number of microfilariae in the circulating blood and the number of adult worms recovered from the subcutaneous connective tissue. At necropsy 300 days after infection living female parasites could not be found any more.
Topics: Animals; Arachnid Vectors; Dexamethasone; Dipetalonema; Dipetalonema Infections; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Filariasis; Male; Microfilariae; Rats; Ticks; Time Factors
PubMed: 571635
DOI: No ID Found -
Experimental Parasitology Jun 1980
Comparative Study
Topics: Animals; Dipetalonema; Dipetalonema Infections; Female; Filariasis; Host-Parasite Interactions; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Nude; Microfilariae; Species Specificity; T-Lymphocytes
PubMed: 6966226
DOI: 10.1016/0014-4894(80)90074-0 -
Parasitology Feb 1978
Topics: Animals; Blood; Cricetinae; Dipetalonema; Dipetalonema Infections; Female; Filariasis; Immune Sera; Immunization; Male; Mesocricetus; Microfilariae
PubMed: 564017
DOI: 10.1017/s0031182000047399 -
The Southeast Asian Journal of Tropical... Mar 1980
Topics: Animals; Dipetalonema; Dipetalonema Infections; Female; Filariasis; Haplorhini; Malaysia; Male; Monkey Diseases; Species Specificity
PubMed: 6773151
DOI: No ID Found -
Canadian Journal of Zoology Mar 1965
Topics: Animals; Carnivora; Dipetalonema; Filarioidea; Mephitidae; Microfilariae; Pathology; Rabies; Rabies Vaccines; Research
PubMed: 14322432
DOI: 10.1139/z65-030 -
The Journal of Parasitology Apr 1974
Topics: Animals; Blood; Cricetinae; Dipetalonema; Female; Gerbillinae; Larva; Male; Nematode Infections; Sex Factors; Ticks; Time Factors
PubMed: 4856593
DOI: No ID Found