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The American Journal of Tropical... Sep 1967
Topics: Dipetalonema; Female; Filariasis; Guyana; Humans; Indians, South American; Male; Mansonella
PubMed: 6069567
DOI: 10.4269/ajtmh.1967.16.628 -
Acta Tropica Oct 1997To assess the current status of Dirofilaria immitis infection and to determine whether there were other canine filarial infections in north Taiwan, postmortem...
To assess the current status of Dirofilaria immitis infection and to determine whether there were other canine filarial infections in north Taiwan, postmortem examination was conducted in 180 stray dogs more than 12 months old. Blood and serum samples were examined using a modified Knott's test and an antigen-detecting enzyme-linked immunosorbent assay (ELISA) kit, respectively. Filarial infection was found in 60.6% of the dogs: 55% with D. immitis and 12.2% Dipetalonema reconditum. Moreover, the ELISA was determined to be more sensitive than the Knott's test. Although canine heartworm infection in Taiwan has been attributed to the unrestricted import of dogs from endemic areas, the results of this study indicate that transmission of D. immitis and Dip. reconditum may occur indigenously in the local canine population. This study is also the first record of Dip. reconditum in Taiwan.
Topics: Animals; Dipetalonema Infections; Dirofilariasis; Dog Diseases; Dogs; Enzyme-Linked Immunosorbent Assay; Female; Male; Prevalence; Taiwan
PubMed: 9352007
DOI: 10.1016/s0001-706x(97)00081-8 -
Veterinary Parasitology Jun 2002Both Dirofilaria immiti and Dipetalonema reconditum may be found in blood of infected dogs but it is not easy to distinguish D. immitis from D. reconditum in morphology....
Specific polymerase chain reaction for differential diagnosis of Dirofilaria immitis and Dipetalonema reconditum using primers derived from internal transcribed spacer region 2 (ITS2).
Both Dirofilaria immiti and Dipetalonema reconditum may be found in blood of infected dogs but it is not easy to distinguish D. immitis from D. reconditum in morphology. We cloned and sequenced the contiguous internal transcribed spacer (ITS) region, ITS1-5.8S-ITS2, of these two different parasites and published on GenBank as AF217800 for D. immiti and AF217801 for D. reconditum in this study. We designed two pairs of specific primers derived from ITS2 being used for polymerase chain reaction (PCR). The amplicons of ITS2 from D. immiti and D. reconditum are 302 and 348bp, respectively. Moreover, the limitation for amplifying ITS2 gene using this PCR demonstrated that 1 x 10(-2) microfilaria of each species of parasite smashed or even with mixed samples could be detected and the PCR products were predicted as the same as that described above. Thus, D. immiti and D. reconditum could be differentially diagnosed by this specific PCR. Seventeen clinical cases were evaluated and all of them were correctly identified. In this study, ITS1-5.8S-ITS2 of D. immiti or D. reconditum were the first time sequenced and analyzed. No significant similarity of ITS1 and ITS2 between D. immiti and D. reconditum could be observed.
Topics: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Helminth; DNA, Ribosomal Spacer; Diagnosis, Differential; Dipetalonema; Dipetalonema Infections; Dirofilaria immitis; Dirofilariasis; Dog Diseases; Dogs; Molecular Sequence Data; Polymerase Chain Reaction; Sequence Alignment
PubMed: 12062512
DOI: 10.1016/s0304-4017(02)00032-8 -
Transactions of the Royal Society of... 1980
Topics: Animals; Antinematodal Agents; Benzimidazoles; Dipetalonema; Dipetalonema Infections; Filariasis; Gerbillinae; Mebendazole; Rodent Diseases
PubMed: 7193927
DOI: 10.1016/0035-9203(80)90222-9 -
Dipetalonema viteae: resistance in Meriones unguiculatus with multiple infections of stage-3 larvae.Experimental Parasitology Oct 1986The jird, Meriones unguiculatus, infected with 80 normal infective larvae of Dipetalonema viteae, revealed a recovery rate of 27.9% 12 weeks after infection. A...
The jird, Meriones unguiculatus, infected with 80 normal infective larvae of Dipetalonema viteae, revealed a recovery rate of 27.9% 12 weeks after infection. A pretreatment by three injections of 50 normal larvae each and challenge by 80 larvae resulted in a recovery rate of 10.7%. The recovered worms were longer than those from the challenge control animals. When three times 50 irradiated larvae (35 krad) were inoculated, the recovery rate of the challenge decreased to 2.6%, representing a protection of 90.7%. The surviving adult worms were stunted and derived exclusively from the 80 normal larvae given for challenge, since absolutely no adult worms were recovered in eight animals inoculated three times with 50 irradiated larvae only. Sera of all pretreated jirds contained IgG and IgM antibodies which bound in immunoblotting experiments bound predominantly to three proteins of larvae with molecular masses of 68,140, and 165 kDa, respectively. Enzymatic surface iodination revealed that the three antigens were exposed on the larval surface. The coincidence of a partial resistance to a challenge infection and of an antibody response against surface proteins of infective larvae suggests an importance of these antigens for the rejection of D. viteae mediated by an acquired immunological resistance of M. unguiculatus.
Topics: Animals; Antigens, Helminth; Antigens, Surface; Dipetalonema; Dipetalonema Infections; Filariasis; Gerbillinae; Immunity, Active; Immunoglobulin G; Immunoglobulin M; Proteins
PubMed: 3743715
DOI: 10.1016/0014-4894(86)90028-7 -
Veterinary Research Communications 1996
Topics: Animals; Dipetalonema Infections; Dirofilaria; Dirofilariasis; Dog Diseases; Dogs; Europe; Prevalence
PubMed: 8865570
DOI: 10.1007/BF00366534 -
Tropical and Geographical Medicine Jan 1989The study of haemoparasites in our blood donors revealed the following parasite prevalences: microfilaria (mf) of Loa loa (1.3%); Dipetalonema perstans (15.6%); Loa loa...
The study of haemoparasites in our blood donors revealed the following parasite prevalences: microfilaria (mf) of Loa loa (1.3%); Dipetalonema perstans (15.6%); Loa loa and D. perstans (0.2%), Plasmodium falciparum (3.3%), Plasmodium malariae (1.0%) and a mixture of P. falciparum and P. malariae (0.2%). No trypanosomes were observed in the 480 blood samples screened. There were more cases of D. perstans infection in the 24-30 year age group, indicating their increased exposure frequencies. Matching of ABO blood group and mf infection rates shows that the O blood group has a higher prevalence rate with regard to D. perstans than Loa loa. The B blood group, however, had the highest cumulative % of mf infection (23.4%), but these values are consistent with the preponderance of the various blood groups in the study population. Commercial donors, most of whom come from the less affluent social classes, had higher prevalence rates of mf. It is recommended that blood be properly screened for mf before donation for transfusion. Recipients of infected blood should be followed up so that any consequent infection would be treated immediately.
Topics: Adolescent; Adult; Blood Donors; Blood Group Antigens; Filariasis; Humans; Loiasis; Malaria; Mass Screening; Nigeria
PubMed: 2763348
DOI: No ID Found -
Acta Tropica Mar 1987
Topics: Animals; Antibodies; Dipetalonema; Dipetalonema Infections; Filariasis; Muridae
PubMed: 2884834
DOI: No ID Found -
Journal of Helminthology Jun 1982Water decoction of the leaves of Andrographis paniculata killed in vitro the microfilaria of Dipetalonema reconditum in 40 min. Three subcutaneous injections of the...
Water decoction of the leaves of Andrographis paniculata killed in vitro the microfilaria of Dipetalonema reconditum in 40 min. Three subcutaneous injections of the extract into infected dogs at 0.06 ml per kg body-weight reduced the number of microfilariae in blood by more than 85%. The larvae were not totally eliminated with more infections but the reduced microfilarial level persisted. No toxic effect of the extract was observed in rabbits. The treated dogs became lethargic initially for a week, probably due to the mass killing of microfilariae.
Topics: Animals; Anthelmintics; Dipetalonema Infections; Dogs; Drug Evaluation, Preclinical; Female; Filaricides; India; Male; Microfilariae; Plant Extracts; Plants, Medicinal; Rabbits
PubMed: 7201486
DOI: 10.1017/s0022149x0003426x -
Journal of the American Veterinary... Mar 1971
Topics: Acid Phosphatase; Animals; Diagnosis, Differential; Dipetalonema; Dirofilariasis; Dog Diseases; Dogs; Female; Filarioidea; Histocytochemistry; Male; Nematode Infections
PubMed: 5101887
DOI: No ID Found