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Biochemical Pharmacology Nov 1982Male mice treated for 2 weeks with the anticholinesterase insecticide disulfoton (O,O-diethyl S-[2-(ethylthio)-ethyl] phosphorodithioate; 10 mg per kg per day) became...
Male mice treated for 2 weeks with the anticholinesterase insecticide disulfoton (O,O-diethyl S-[2-(ethylthio)-ethyl] phosphorodithioate; 10 mg per kg per day) became tolerant to the hypothermic and antinociceptive effects of disulfoton itself and of oxotremorine, a muscarinic cholinergic agonist. Homogenates of brain and ileum from tolerant animals exhibited reduced binding of the specific muscarinic antagonist [3H]quinuclidinyl benzilate ([3H]QNB). In forebrains of tolerant animals, the number of receptors (Bmax) was decreased 40% with no change in the affinity constant. Acetylcholinesterase (AChE) activity was 15% of control. Forty-eight hours after a single injection of disulfoton (10 mg/kg) mice were more resistant than their controls to the hypothermic and antinociceptive effects of a second administration of the same insecticide and of oxotremorine. Tolerance was not present 96 hr after a single administration of disulfoton. A single injection of disulfoton produced 74, 65 and 27% inhibition of AChE activity after 4, 48 and 96 hr respectively. Four hours after a second injection at 49 and 96 hr, 73 or 72% inhibition was found. [3H]QNB binding of animals treated with a single injection of disulfoton and of controls did not differ at either time point. An increase in the Ki for inhibition of [3H]QNB binding by unlabeled oxotremorine was observed in forebrain from mice killed 48 hr after a single injection of disulfoton, indicating a decreased affinity of the muscarinic receptor for agonists. Binding of [3H]oxotremorine-M was decreased significantly 48 hr after a single injection of disulfoton and after chronic treatment. It is suggested that a differential down-regulation of muscarinic receptors occurs in acute and chronic tolerance, involving agonist and antagonist binding sites and depending on duration of exposure.
Topics: Acetylcholinesterase; Analgesics; Animals; Body Temperature; Brain; Disulfoton; Drug Tolerance; Ileum; Insecticides; Male; Mice; Oxotremorine; Quinuclidinyl Benzilate; Receptors, Cholinergic; Receptors, Muscarinic; Time Factors
PubMed: 7150362
DOI: 10.1016/0006-2952(82)90619-0 -
Bulletin of Environmental Contamination... Jan 1970
PubMed: 24185714
DOI: 10.1007/BF01545114 -
Toxicology and Applied Pharmacology Sep 1982
Topics: Acetylcholinesterase; Animals; Avoidance Learning; Brain; Choline; Disulfoton; Drug Tolerance; Electroshock; Hippocampus; Insecticides; Male; Memory; Mice; Quinuclidinyl Benzilate
PubMed: 7157376
DOI: 10.1016/0041-008x(82)90390-8 -
Bulletin of Environmental Contamination... Apr 1981
Topics: Adult; Cholinesterases; Disulfoton; Environmental Exposure; Erythrocytes; Humans; Insecticides; Male; Middle Aged; Organophosphorus Compounds; Phorate
PubMed: 7236911
DOI: 10.1007/BF01622131 -
Toxicology and Applied Pharmacology Sep 1981
Topics: Acetylcholinesterase; Animals; Body Weight; Disulfoton; Drug Tolerance; Insecticides; Kinetics; Male; Mice; Proteins; Quinuclidines; Quinuclidinyl Benzilate; Receptors, Cholinergic; Receptors, Muscarinic
PubMed: 7292482
DOI: 10.1016/0041-008x(81)90329-x -
Journal of Economic Entomology Jun 1971
Topics: Animals; Coleoptera; Insecticides; Larva; Maryland; Metamorphosis, Biological; Organothiophosphorus Compounds; Phosphoric Acids; Plants, Toxic; Soil; Nicotiana
PubMed: 5558292
DOI: 10.1093/jee/64.3.766a -
Talanta May 2006A technique for the analysis of organophosphorus pesticides (ethoprop, diazinon, disulfoton, fenthion) in aqueous sample using liquid-phase microextraction (LPME),...
Determination of ethoprop, diazinon, disulfoton and fenthion using dynamic hollow fiber-protected liquid-phase microextraction coupled with gas chromatography-mass spectrometry.
A technique for the analysis of organophosphorus pesticides (ethoprop, diazinon, disulfoton, fenthion) in aqueous sample using liquid-phase microextraction (LPME), coupled with gas chromatography-mass spectrometry (GC-MS) was developed. A small section of a hollow fiber inserted into the needle of GC syringe and filled with the 3.5mul of organic solvent was used to extract pesticides from a 20ml aqueous sample. The limits of detection (LOD) with the selected ion monitoring (SIM) mode varied from 0.2 to 0.006mug/l. The calibration curves were linear over three orders of magnitude with R(2)>/=0.996. The relative standard deviations of the analysis (inter- and intra-day) were 5-8%, and the relative recoveries from the lake water sample were greater than 83%. The results were compared with results obtained using solid-phase microextraction (SPME/GC/MS).
PubMed: 18970620
DOI: 10.1016/j.talanta.2005.10.042 -
Journal of Chromatography Oct 1969
Topics: Chromatography, Gas; Chromatography, Thin Layer; Coloring Agents; Enzyme Inhibitors; Oxidation-Reduction; Phosphoric Acids; Sulfones; Sulfoxides
PubMed: 4981072
DOI: 10.1016/s0021-9673(01)92499-9 -
Toxicology and Applied Pharmacology Mar 1969
Topics: Animals; Atropine; Drug Synergism; Liver; Phosphoric Acids; RNA; Rats; Staining and Labeling
PubMed: 4181886
DOI: 10.1016/0041-008x(69)90119-7 -
Life Sciences. Pt. 1: Physiology and... Apr 1971
Topics: Acetylcholinesterase; Animals; Brain; Cell Membrane Permeability; Copper; Ferricyanides; Glycine; Mice; Phosphoric Acids; Rats; Staining and Labeling
PubMed: 4102560
DOI: 10.1016/0024-3205(71)90106-8