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Pharmaceutical Research Sep 2017To utilize nanoparticles produced by condensation of zymosan (an immunotherapeutic polysaccharide) with pegylated polyethylenimine (PEG-PEI) for dual intervention in...
OBJECTIVE
To utilize nanoparticles produced by condensation of zymosan (an immunotherapeutic polysaccharide) with pegylated polyethylenimine (PEG-PEI) for dual intervention in breast cancer by modulating tumor microenvironment and direct chemotherapy.
METHOD
Positively charged PEG-PEI and negatively charged sulphated zymosan were utilized for electrostatic complexation of chemoimmunotherapeutic nanoparticles (ChiNPs). ChiNPs were loaded with doxorubicin hydrochloride (DOX) for improved delivery at tumor site and were tested for in-vivo tolerability. Biodistribution studies were conducted to showcase their effective accumulation in tumor hypoxic regions where tumor associated macrophages (TAMs) are preferentially recruited.
RESULTS
ChiNPs modulated TAMs differentiation resulting in decrement of CD206 positive population. This immunotherapeutic action was furnished by enhanced expression of Th1 specific cytokines. ChiNPs also facilitated an anti-angiogenetic effect which further reduces the possibility of tumor progression and metastasis.
Topics: Animals; Antibiotics, Antineoplastic; Breast; Breast Neoplasms; Cell Proliferation; Cytokines; Doxorubicin; Drug Carriers; Drug Delivery Systems; Female; Immunologic Factors; Macrophages; Mice, Inbred BALB C; Nanoparticles; Polyethyleneimine; Static Electricity; Tissue Distribution; Zymosan
PubMed: 28608139
DOI: 10.1007/s11095-017-2195-2 -
Report on Carcinogens : Carcinogen... 2002
Topics: Animals; Carcinogens; Doxorubicin; Government Regulation; Health Occupations; Humans; Occupational Exposure; United States
PubMed: 15317113
DOI: No ID Found -
Colloids and Surfaces. B, Biointerfaces Sep 2016The hydrophilic drug Doxorubicin hydrochloride (DOX) paired with oleic acid (OA) was successfully incorporated into nanostructured lipid carriers (NLCs) by a...
The hydrophilic drug Doxorubicin hydrochloride (DOX) paired with oleic acid (OA) was successfully incorporated into nanostructured lipid carriers (NLCs) by a high-pressure homogenization (HPH) method. Drug nanovehicles with proper physico-chemical characteristics (less than 200nm with narrow size distribution, spherical shape, layered internal organization, and negative electrical charge) were prepared and characterized by dynamic light scattering, zeta potential measurements, transmission electron microscopy, small-angle X-ray scattering and differential scanning calorimetry. The drug loading and entrapment efficiency of DOX-OA/NLCs were 4.09% and 97.80%, respectively. A pH-dependent DOX release from DOX-OA/NLCs, i.e., fast at pH 3.8 and 5.7 and sustained at pH 7.4, was obtained. A cytotoxicity assay showed that DOX-OA/NLCs had comparable cytotoxicity to pure DOX and were favorably taken up by HCT 116 cells. The intracellular distribution of DOX was also studied using a confocal laser scanning microscope. All of these results demonstrated that DOX-OA/NLCs could be a promising drug delivery system with tumor-specific DOX release for cancer treatment.
Topics: Doxorubicin; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Nanostructures; Oleic Acid
PubMed: 27137808
DOI: 10.1016/j.colsurfb.2016.04.027 -
ACS Biomaterials Science & Engineering Oct 2021Iron-based metal-organic frameworks (MOFs) have been reported to have great potential for encapsulating doxorubicin hydrochloride (DOX), which is a frequently used...
Iron-based metal-organic frameworks (MOFs) have been reported to have great potential for encapsulating doxorubicin hydrochloride (DOX), which is a frequently used anthracycline anticancer drug. However, developing a facile approach to realize high loading capacity and efficiency as well as controlled release of DOX in MOFs remains a huge challenge. Herein, we synthesized water-stable MIL-101(Fe)-CH through a microwave-assisted method. It was found the nano-MOFs acted as nanosponges when soaked in a DOX alkaline aqueous solution with a loading capacity experimentally up to 24.5 wt %, while maintaininga loading efficiency as high as 98%. The mechanism of the interaction between DOX and nanoMOFs was investigated by absorption spectra and density functional theory (DFT) calculations, which revealed that the deprotonated DOX was electrostatically adsorbed to the unsaturated FeOCl(COO)·HO (named Fe trimers). In addition, the as-designed poly(ethylene glycol--propylene glycol) (F127) modified nanoparticles (F127-DOX-MIL) could be decomposed under the stimulation of glutathione (GSH) and ATP. As a result, DOX and Fe(III) ions were released, and they could undergo a Fenton-like reaction with the endogenous HO to generate the highly toxic hydroxyl radical (·OH). The in vitro experiments indicated that F127-DOX-MIL could cause remarkable Hela cells inhibition through chemotherapy and chemodynamic therapy. Our study provides a new strategy to design a GSH/ATP-responsive drug-delivery nanosystem for chemo/chemodynamic therapy.
Topics: Doxorubicin; Ferric Compounds; HeLa Cells; Humans; Hydrogen Peroxide; Metal-Organic Frameworks
PubMed: 34550683
DOI: 10.1021/acsbiomaterials.1c00874 -
Bioelectrochemistry (Amsterdam,... Jun 2021A glassy carbon electrode modified with multi-walled carbon nanotubes (MWCNTs) decorated with gold nanoparticles has been investigated for the first time as an...
A glassy carbon electrode modified with multi-walled carbon nanotubes (MWCNTs) decorated with gold nanoparticles has been investigated for the first time as an ultrasensitive electrochemical sensor for the determination of doxorubicin hydrochloride (DOX), an efficient antitumor agent. The developed nanocomposite has been characterized by scanning electron microscopy (SEM), besides cyclic and linear sweep voltammetry electrochemical techniques. An efficient catalytic activity for the reduction of DOX has been demonstrated, leading to a significant increase in peak current density and a remarkable decrease in reduction over-potential. Under the optimal condition, a wide linear DOX concentration range from 1×10 to 1×10 M with a very low detection limit of 6.5 pM was achieved with the modified electrode. Meanwhile, the functionalized MWCNTs/gold nanoparticles indicated an appropriate selectivity, reproducibility, and repeatability as well as long-term stability. The promising outcomes of this research approved the applicability of the developed nanocomposite sensor towards trace amounts of DOX in pharmaceutical and clinical preparations.
Topics: Antineoplastic Agents; Biosensing Techniques; Doxorubicin; Electrochemical Techniques; Electrodes; Gold; Limit of Detection; Metal Nanoparticles; Nanocomposites; Nanotubes, Carbon; Sensitivity and Specificity
PubMed: 33524656
DOI: 10.1016/j.bioelechem.2021.107741 -
Talanta Dec 2023Doxorubicin (DOX) is a highly effective anticancer drug with a narrow therapeutic window; thus, sensitive and timely detection of DOX is crucial. Using electrodeposition...
Doxorubicin (DOX) is a highly effective anticancer drug with a narrow therapeutic window; thus, sensitive and timely detection of DOX is crucial. Using electrodeposition of silver nanoparticles (AgNPs) and electropolymerization of alginate (Alg) layers on the surface of a glassy carbon electrode, a novel electrochemical probe was constructed (GCE). The fabricated AgNPs/poly-Alg-modified GCE probe was utilized for the quantification of DOX in unprocessed human plasma samples. For the electrodeposition of AgNPs and electropolymerization of alginate (Alg) layers on the surface of GCE, cyclic voltammetry (CV) was used in the potential ranges of -2.0 to 2.0 V and -0.6 to 0.2 V, respectively. The electrochemical activity of DOX exhibited two oxidation processes at the optimum pH value of 5.5 on the surface of the modified GCE. The DPV spectra of poly(Alg)/AgNPs modified GCE probe toward consecutive concentrations of DOX in plasma samples demonstrated wide dynamic ranges of 15 ng/mL-0.1 μg/mL and 0.1-5.0 μg/mL, with a low limit of quantification (LLOQ) of 15 ng/mL. The validation results indicated that the fabricated electrochemical probe might serve as a highly sensitive and selective assay for the quantification of DOX in patient samples. As an outstanding feature, the developed probe could detect DOX in unprocessed plasma samples and cell lysates without the requirement for pretreatment.
Topics: Humans; Carbon; Doxorubicin; Metal Nanoparticles; Silver; Biofouling; Electrodes; Alginates; Electrochemical Techniques; Limit of Detection
PubMed: 37379752
DOI: 10.1016/j.talanta.2023.124846 -
International Journal of Biological... Sep 2018In the present study, the potential of doxorubicin hydrochloride (DOX)-loaded electrospun chitosan/cobalt ferrite/titanium oxide nanofibers was studied to investigate...
In the present study, the potential of doxorubicin hydrochloride (DOX)-loaded electrospun chitosan/cobalt ferrite/titanium oxide nanofibers was studied to investigate the simultaneous effect of hyperthermia and chemotherapy against melanoma cancer B16F10 cell lines. The cobalt ferrite nanoparticles were synthesized via microwave heating method. The titanium oxide nanoparticles were mixed with cobalt ferrite to control the temperature rise. The synthesized nanoparticles and nanofibers were characterized using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and vibrating sample magnetometer (VSM) analysis. The DOX loading efficiency and in vitro drug release of DOX from nanofibers were investigated at both physiological and acidic conditions by an alternating of magnetic field and without magnetic field effect. The fastest release of DOX from prepared magnetic nanofibers was observed at acidic pH by alternating of magnetic field. The antitumor activity of synthesized nanofibers was also investigated on the melanoma cancer B16F10 cell lines. The obtained results revealed that the DOX loaded-electrospun chitosan/cobalt ferrite/titanium oxide nanofibers can be used for localized cancer therapy.
Topics: Animals; Antineoplastic Agents; Cell Line, Tumor; Chitosan; Cobalt; Delayed-Action Preparations; Doxorubicin; Drug Liberation; Ferric Compounds; Magnetics; Melanoma, Experimental; Mice; Nanofibers; Nanoparticles; Temperature; Titanium
PubMed: 29723626
DOI: 10.1016/j.ijbiomac.2018.04.161 -
Zhongguo Shi Yan Xue Ye Xue Za Zhi Aug 2021To evaluate the efficacy and safety of CHOP regimen based on doxorubicin hydrochloride liposome in the initial treatment of elderly patients with diffuse large B-cell...
OBJECTIVE
To evaluate the efficacy and safety of CHOP regimen based on doxorubicin hydrochloride liposome in the initial treatment of elderly patients with diffuse large B-cell lymphoma (DLBCL).
METHODS
Thirty-one patients with DLBCL treated from January 1, 2012 to December 31, 2019 were analyzed retrospectively, their median age was 83 (71-95) years old, and all of them were in Ⅲ-Ⅳ stage, including 17 cases who had international prognostic index (IPI) ≥ 3. The patients were treated with R-CHOP and CHOP regimens based on doxorubicin hydrochloride liposome. The efficacy and safety were evaluated during and after treatment.
RESULTS
A total of 219 chemotherapy cycles and 7 median cycles were performed in 31 patients. The overall response (OR) rate and complete remission (CR) rate was 80.7% (25/31) and 61.3% (19/31), respectively, as well as 2 cases (6.5%) stable, 4 cases (12.9%) progressive. The main toxicities were as follows: the incidence of grade Ⅲ -Ⅳ neutropenia was 29% (9/31); two patients (6.5%) developed degree Ⅰ-Ⅱ cardiac events, which were characterized by new degree Ⅰ atrioventricular block; there were no cardiac events requiring emergency treatment and discontinuation of chemotherapy. The 1-year, 2-year and 3-year overall survival rate was 83.9%, 77.4% and 61.3%, respectively. The 1-year, 2-year and 3-year progression-free survival rate was 77.4%, 64.5% and 61.3%, respectively.
CONCLUSION
The chemotherapy regimen based on doxorubicin hydrochloride liposome has better efficacy and higher cardiac safety for elderly patients with DLBCL.
Topics: Aged; Aged, 80 and over; Antineoplastic Combined Chemotherapy Protocols; Cyclophosphamide; Doxorubicin; Humans; Liposomes; Lymphoma, Large B-Cell, Diffuse; Prednisolone; Prednisone; Retrospective Studies; Rituximab; Vincristine
PubMed: 34362493
DOI: 10.19746/j.cnki.issn.1009-2137.2021.04.018 -
Journal of Biomedical Nanotechnology Nov 2016Mesoporous silica nanoparticles (MSNs) are widely used nanoparticles with a pore rich structure that is suitable for drug delivery. Here, we used MSNs to carry and...
Mesoporous silica nanoparticles (MSNs) are widely used nanoparticles with a pore rich structure that is suitable for drug delivery. Here, we used MSNs to carry and deliver doxorubicin hydrochloride (DOX) in vivo to study the features of DOXloaded MSNs (MSNs@DOX). We used TEM and zeta potential to illustrate that MSNs@DOX increase apoptosis and decrease metastasis of tumor cells. We used MTT, flow cytometry, Western blotting, wound healing, and transwell assays, as well as an in vivo metastasis model to explore the anti-carcinoma efficacy of MSNs@DOX. Our results showed that DOX was efficiently loaded into MSNs measuring approximately 88±11 nm, which significantly increased the antitumor efficacy of DOX on lung cancer, both in vitro and in vivo, compared to a regular DOX treatment. MSNs@DOX markedly induced apoptosis through cytochrome C release and the caspase family. Furthermore, cell migration and invasion were sharply inhibited, both in vitro and in vivo. We also found that the enhanced effect of MSNs@DOX might be due to an increased cellular uptake by tumor cells based on the enhanced permeability and retention time of the nanoparticles. Matrigel plug assays and Western blotting assays revealed that the molecular mechanism behind the anti-metastasis effect might be attributed to the suppression of VEGF-mediated angiogenesis. Our results offer a new perspective on the application of nanoparticles against metastasis.
Topics: Animals; Antineoplastic Agents; Apoptosis; Carcinoma, Non-Small-Cell Lung; Cell Movement; Doxorubicin; Drug Carriers; Female; Lung Neoplasms; Mice; Mice, Nude; Nanoparticles; Neovascularization, Pathologic; Particle Size; Silicon Dioxide; Vascular Endothelial Growth Factor A
PubMed: 29363936
DOI: 10.1166/jbn.2016.2290 -
The Western Journal of Medicine Nov 1979Adriamycin (doxorubicin hydrochloride) is an antineoplastic agent effective against a wide range of malignant conditions, although cardiac toxicity, especially... (Review)
Review
Adriamycin (doxorubicin hydrochloride) is an antineoplastic agent effective against a wide range of malignant conditions, although cardiac toxicity, especially dose-dependent cardiomyopathy, limits its long-term use. Previous mediastinal radiation therapy or left ventricular dysfunction and advanced age increase the risk of this complication developing. Unfortunately, there is no readily available, noninvasive method that can predict Adriamycin-induced congestive heart failure (CHF). However, both endomyocardial biopsy and radionuclide ejection-fraction measurement are promising techniques which may soon permit selection of patients who can safely receive this drug. At present, Adriamycin-induced CHF can best be prevented by limiting the total dose as follows: 400 to 450 mg per sq meter following mediastinal radiation and 500 to 550 mg per sq meter for patients without other significant risk factors. Consideration of dose-response data and use of a weekly schedule may soon permit the administration of Adriamycin for long-term antineoplasm therapy.
Topics: Age Factors; Aged; Doxorubicin; Heart Failure; Humans; Neoplasms; Risk
PubMed: 394479
DOI: No ID Found