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Methods in Molecular Biology (Clifton,... 2018The chapter provides a concise account of collecting techniques, and basic histological techniques for investigating planarians, intended for use by practicing...
The chapter provides a concise account of collecting techniques, and basic histological techniques for investigating planarians, intended for use by practicing scientists and postgraduate students new to the field. Selected hands-on methods are described that will lead to well-stained preparations suitable for light microscopic examination for subsequent taxonomic identification or general investigations into planarian functional cytology and histology. Major equipment items that are minimal essential requirements of a functional histological laboratory are indicated. The chapter includes sectioning and staining troubleshooting guides that will be very helpful for those who must work in the laboratory without the opportunity to consult qualified and experienced technicians or microscopists.
Topics: Animals; Histological Techniques; Laboratories; Microscopy; Planarians; Staining and Labeling; Students
PubMed: 29916162
DOI: 10.1007/978-1-4939-7802-1_9 -
International Review of Cytology 1969
Topics: Freeze Etching; Freezing; Histological Techniques; Methods; Microscopy, Electron; Microtomy
PubMed: 4919571
DOI: 10.1016/s0074-7696(08)60209-0 -
Histology and Histopathology Aug 2015The repair of articular cartilage defects is increasingly moving into the focus of experimental and clinical investigations. Histological analysis is the gold standard... (Review)
Review
The repair of articular cartilage defects is increasingly moving into the focus of experimental and clinical investigations. Histological analysis is the gold standard for a valid and objective evaluation of cartilaginous repair tissue and predominantly relies on the use of established scoring systems. In the past three decades, numerous elementary and complex scoring systems have been described and modified, including those of O'Driscoll, Pineda, Wakitani, Sellers and Fortier for entire defects as well as those according to the International Cartilage Repair Society (ICRS-I/II) for osteochondral tissue biopsies. Yet, this coexistence of different grading scales inconsistently addressing diverse parameters may impede comparability between reported study outcomes. Furthermore, validation of these histological scoring systems has only seldom been performed to date. The aim of this review is (1) to give a comprehensive overview and to compare the most important established histological scoring systems for articular cartilage repair, (2) to describe their specific advantages and pitfalls, and (3) to provide valid recommendations for their use in translational and clinical studies of articular cartilage repair.
Topics: Biopsy; Cartilage, Articular; Chondrocytes; Histological Techniques; Humans; Regeneration; Reproducibility of Results; Wound Healing
PubMed: 25876650
DOI: 10.14670/HH-11-620 -
Journal of Histotechnology Sep 2019Humans and machines both have an inherent error rate. As long as this is true, sub-optimal events will occur in the histology laboratory. The best approach to... (Review)
Review
Humans and machines both have an inherent error rate. As long as this is true, sub-optimal events will occur in the histology laboratory. The best approach to troubleshooting and remedying these events is to (1) understand the various theories of action behind histology procedures and stains then (2) apply a problem-solving mentality to develop a corrective action. These theories and problem solving strategies are presented in this review article.
Topics: Artifacts; Diagnostic Errors; Histological Techniques; Humans; Specimen Handling
PubMed: 31379299
DOI: 10.1080/01478885.2019.1640923 -
Brazilian Journal of Biology = Revista... May 2001The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were...
The objective of the present study was to adapt techniques for the histological processing of Dugesiidae cocoons for the study of embryo development. The cocoons were fixed with formalin, SUSA, Bouin or paraformaldehyde/glutaraldehyde and subsequently embedded in Paraplast or glycol methacrylate (Historesin). Paraplast embedding yielded reasonable results only after the cocoon was perforated or fixed for a prolonged period of time using softening techniques with acid solutions. When the SUSA or Bouin fixative and Historesin embedding techniques were used the results were good for light microscopical analysis. Fixation with paraformaldehyde/glutaraldehyde and glycol methacrylate embedding resulted in better tissue preservation, and did not require prolonged fixation or softening techniques. Thus, we suggest this technique for light microscopical analysis of embryo development in Dugesiidae.
Topics: Animals; Fixatives; Formaldehyde; Histological Techniques; Methacrylates; Platyhelminths; Tissue Fixation
PubMed: 11514904
DOI: 10.1590/s0034-71082001000200019 -
The International Journal of... Mar 2017The last decade has seen a proliferation of tissue clearing methods that render large biological samples transparent and allow unprecedented three-dimensional views of... (Review)
Review
The last decade has seen a proliferation of tissue clearing methods that render large biological samples transparent and allow unprecedented three-dimensional views of enormous volumes of tissue. For a scientist wondering whether these methods will be useful to address their research problems, it can be bewildering to sort through the ever-increasing number of papers introducing new clearing methods. Here, I provide a concise summary for the novice describing what tissue clearing is, which research problems it can be applied to, how to decide on a clearing method, and where the field is headed in the future.
Topics: Animals; Histological Techniques; Hydrogels; Solutions; Solvents; Staining and Labeling; Tissue Embedding
PubMed: 28082099
DOI: 10.1016/j.biocel.2016.12.009 -
Morfologiia (Saint Petersburg, Russia) 2007This review of the literature presents the data on the history of development of the methods of staining of histological sections using the natural dye, hematoxylin. The... (Review)
Review
This review of the literature presents the data on the history of development of the methods of staining of histological sections using the natural dye, hematoxylin. The content of solutions of the most commonly used alum hematoxylins is described. The information is given on the modern staining methods, in which alum and iron hematoxylins are used, as well as on the hematoxylins, in which vanadium, lead, bismuth, uranium and other metals' salts are applied as mordants.
Topics: Coloring Agents; Hematoxylin; Histological Techniques; Staining and Labeling
PubMed: 18411731
DOI: No ID Found -
Science (New York, N.Y.) Dec 1965Tissue can be homogenized in conventional ground-glass tissue grinders in conjunction with centrifugation. The method may be of special value for separating soluble from...
Tissue can be homogenized in conventional ground-glass tissue grinders in conjunction with centrifugation. The method may be of special value for separating soluble from insoluble material during homogenization of tissue. As yet only yeast cells and spores of slime mold have been homogenized, but the method may be useful for many tissues.
Topics: Centrifugation; Histological Techniques; In Vitro Techniques; Myxomycetes; Subcellular Fractions; Yeasts
PubMed: 5323417
DOI: 10.1126/science.150.3704.1739 -
Journal of Microscopy Nov 1986Vapour deposition of water is a major problem in cryo-transfer and cryo-observation of frozen specimens. The use of (sliding) shields to protect the specimen during...
Vapour deposition of water is a major problem in cryo-transfer and cryo-observation of frozen specimens. The use of (sliding) shields to protect the specimen during transfer has been evaluated and was found to be a fail-safe procedure. Shielding the specimen with solidified coolant is proposed as an alternative or additional protection. The protective layer (ethane, Freon 22) can be evaporated in the high vacuum of the microscope. The evaporating coolant will carry away water films deposited on top. The temperature of coolant evaporation is low enough to prevent possible devitrification of water in the specimen itself.
Topics: Freezing; Histological Techniques; Mass Spectrometry
PubMed: 3546702
DOI: 10.1111/j.1365-2818.1986.tb02802.x -
Cold Spring Harbor Protocols Dec 2020Increasing use is being made of cell smears for cell-staining studies. Suspension cells can be attached to slides by drying, and cell smears can also be prepared from...
Increasing use is being made of cell smears for cell-staining studies. Suspension cells can be attached to slides by drying, and cell smears can also be prepared from biopsy samples, such as needle aspirates, tissue scrapings, or freshly dissected tissues. In these procedures, a thin layer of cells is deposited on a dry slide by physical methods. The most important factor in obtaining good staining patterns is that the smear be only a single cell thick. Tissue smears do not preserve tissue architecture, but are useful for identifying pathological changes and infectious organisms in tissue samples. Cell smears are easily prepared and can be fixed readily by any of the methods used for attached cells.
Topics: Antibodies; Biopsy, Needle; Histological Techniques; Humans; Immunohistochemistry; Staining and Labeling; Tissue Fixation
PubMed: 33262235
DOI: 10.1101/pdb.prot099630