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International Journal of Systematic and... Dec 2018A Gram-stain-positive, rod-shaped bacterial strain, 22-7, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach....
A Gram-stain-positive, rod-shaped bacterial strain, 22-7, was isolated from ocean sediment of Laizhou Bay, China, and was characterized by using a polyphasic approach. Optimal growth was observed at 33 °C on a 2216E agar plate of pH 7.5 and with 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences identified it as a member of the genus Jeotgalibacillus, most similar to Jeotgalibacillus campisalis SF-57 (98.7 % similarity), Jeotgalibacillus marinus DSM 1297 (98.2 %) and Jeotgalibacillus soli P9 (97.1 %). Average nucleotide identity values and digital DNA-DNA hybridization values were less than 74.2 and 18.1 %, respectively, between strain 22-7 and the type strains of closely related species. The major polar lipids were aminophospholipid, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major fatty acids (>10 %) were anteiso-C15 : 0 and iso-C15 : 0; and the major menaquinone was MK-7. The peptidoglycan type of the cell wall was A1α linked through l-lysine as the diamino acid. Combined data from phenotypic, chemotaxonomic and genotypic characterizations demonstrated that strain 22-7 represents a novel Jeotgalibacillus species, for which the name Jeotgalibacillus proteolyticus sp. nov. is proposed. The type strain is 22-7(=MCCC 1H00228=KCTC 33930).
Topics: Bacterial Typing Techniques; Base Composition; Cell Wall; China; DNA, Bacterial; Fatty Acids; Geologic Sediments; Nucleic Acid Hybridization; Peptide Hydrolases; Peptidoglycan; Phospholipids; Phylogeny; Planococcaceae; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Vitamin K 2
PubMed: 30328805
DOI: 10.1099/ijsem.0.003060 -
Antonie Van Leeuwenhoek Jun 2022A Gram-stain-positive, orange-pigmented, rod-shaped and flagellated bacterial strain T12 was isolated from wetland soil in Kunyu Mountain Wetland in Yantai, China. The...
A Gram-stain-positive, orange-pigmented, rod-shaped and flagellated bacterial strain T12 was isolated from wetland soil in Kunyu Mountain Wetland in Yantai, China. The strain was able to grow at 15-40 °C (optimum 37 °C), at 0.0-9.0% NaCl (optimum 2%, w/v) and at pH 5.5-9.0 (optimum 8.5). A phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain T12 is a member of the family Planococcaceae, sharing 97.6% and 97.1% sequence similarity with the type strains of Jeotgalibacillus salarius and Jeotgalibacillus marinus, respectively. Genome-based analyses revealed a genome size of 3,506,682 bp and a DNA G + C content of 43.7%. Besides, the genome sequence led to 55.0-74.6% average amino acid identity values and 67.8-74.7% average nucleotide identity values between strain T12 and the current closest relatives. Digital DNA-DNA hybridization of strain T12 with the type strains of Jeotgalibacillus proteolyticus and J. marinus demonstrated 19.0% and 20.3% relatedness, respectively. The chemotaxonomic analysis showed that the sole quinone was MK-7. The predominant cellular fatty acids were iso-C, anteiso-C, Cω7c alcohol and iso-C. The polar lipids consisted of an unidentified aminolipid, phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Based on the polyphasic characterization, strain T12 is considered to represent a novel species, for which the name Jeotgalibacillus aurantiacus sp. nov. is proposed. The type strain is T12 (= KCTC 43296 = MCCC 1K07171).
Topics: Bacterial Typing Techniques; Carotenoids; China; Citrus sinensis; DNA, Bacterial; Fatty Acids; Multigene Family; Phospholipids; Phylogeny; Planococcaceae; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil; Wetlands
PubMed: 35396624
DOI: 10.1007/s10482-022-01731-x -
Antonie Van Leeuwenhoek May 2024A Gram-stain positive, aerobic, alkalitolerant and halotolerant bacterium, designated HH7-29, was isolated from the confluence of the Fenhe River and the Yellow River in...
A Gram-stain positive, aerobic, alkalitolerant and halotolerant bacterium, designated HH7-29, was isolated from the confluence of the Fenhe River and the Yellow River in Shanxi Province, PR China. Growth occurred at pH 6.0-12.0 (optimum, pH 8.0-8.5) and 15-40℃ (optimum, 32℃) with 0.5-24% NaCl (optimum, 2-9%). The predominant fatty acids (> 10.0%) were iso-C and anteiso-C. The major menaquinones were MK-7 and MK-8. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified phospholipids. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that strain HH7-29 was a member of the genus Jeotgalibacillus, exhibiting high sequence similarity to the 16S rRNA gene sequences of Jeotgalibacillus alkaliphilus JC303 (98.4%), Jeotgalibacillus salarius ASL-1 (98.1%) and Jeotgalibacillus alimentarius YKJ-13 (98.1%). The genomic DNA G + C content was 43.0%. Gene annotation showed that strain HH7-29 had lower protein isoelectric points (pIs) and possessed genes related to ion transport and organic osmoprotectant uptake, implying its potential tolerance to salt and alkali. The average nucleotide identity, digital DNA-DNA hybridization values, amino acid identity values, and percentage of conserved proteins values between strain HH7-29 and its related species were 71.1-83.8%, 19.5-27.4%, 66.5-88.4% and 59.8-76.6%, respectively. Based on the analyses of phenotypic, chemotaxonomic, phylogenetic and genomic features, strain HH7-29 represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus haloalkalitolerans sp. nov. is proposed. The type strain is HH7-29 (= KCTC 43417 = MCCC 1K07541).
Topics: Phylogeny; RNA, Ribosomal, 16S; China; Rivers; DNA, Bacterial; Base Composition; Fatty Acids; Sodium Chloride; Bacterial Typing Techniques; Phospholipids; Sequence Analysis, DNA; Nucleic Acid Hybridization
PubMed: 38691182
DOI: 10.1007/s10482-024-01968-8 -
International Journal of Systematic and... Mar 2012A Gram-staining-positive, motile, rod-shaped, spore-forming bacterium, designated P9(T), was isolated from soil in Portugal. This organism was aerobic and catalase- and...
A Gram-staining-positive, motile, rod-shaped, spore-forming bacterium, designated P9(T), was isolated from soil in Portugal. This organism was aerobic and catalase- and oxidase-positive. It had an optimum growth temperature of about 35 °C and an optimum growth pH of about 8.0-8.5, and grew in medium with 0-9% (w/v) NaCl. The cell-wall peptidoglycan was of the A1α type, with L-lysine as the diagnostic diamino acid. The major respiratory quinone was menaquinone 7 (MK-7) and the major fatty acids were anteiso-C(15:0) (45.4%), iso-C(15:0) (22.0%) and anteiso-C(17:0) (11.2%). The genomic DNA G+C content was about 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain P9(T) was most closely related to Jeotgalibacillus campisalis DSM 18983(T) (96.8%) and Jeotgalibacillus marinus DSM 1297(T) (96.5%). These two recognized species formed a coherent cluster with strain P9(T) that was supported by a bootstrap value of 99%. On the basis of the phylogenetic analysis and physiological and biochemical characteristics, strain P9(T) (=DSM 23228(T)=LMG 25523(T)) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed.
Topics: Aerobiosis; Bacterial Typing Techniques; Base Composition; Benzoquinones; Catalase; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Hydrogen-Ion Concentration; Lysine; Molecular Sequence Data; Oxidoreductases; Peptidoglycan; Phylogeny; Planococcaceae; Portugal; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sodium Chloride; Soil Microbiology; Temperature
PubMed: 21531739
DOI: 10.1099/ijs.0.028878-0 -
International Journal of Systematic and... Dec 2016A Gram-stain-positive, non-motile, rod-shaped bacterium (strain JC303T) isolated from a salt pan was identified based on 16S rRNA gene sequence analysis as a member of...
Description of Jeotgalibacillus alkaliphilus sp. nov., isolated from a solar salt pan, and Jeotgalibacillus terrae sp. nov., a name to replace 'Jeotgalibacillus soli' Chen et al. 2010.
A Gram-stain-positive, non-motile, rod-shaped bacterium (strain JC303T) isolated from a salt pan was identified based on 16S rRNA gene sequence analysis as a member of the genus Jeotgalibacillus. It was related most closely to Jeotgalibacillus salarius ASL-1T (99.1 % similarity), Jeotgalibacillusalimentarius YKJ-13T (97.9 %), Jeotgalibacillussoli JSM 081008 (97.9 %), Jeotgalibacillusmalaysiensis D5T (97.8 %), Jeotgalibacillusmarinus DSM 1297T (96.3 %), Jeotgalibacilluscampisalis SF-57T (96.1 %) and J. soli P9T (94.9 %). Genomic relatedness based on DNA-DNA hybridization of strain JC303T with the type strains of the closest related species was less than 40 %. Diphosphatidylglycerol, three aminophospholipids, an unidentified aminoglycolipid, two unidentified phospholipids and an unidentified lipid were the polar lipids of strain JC303T. Major (>10 %) fatty acids were anteiso-C15 : 0, iso-C15 : 0 and iso-C14 : 0. Cell-wall amino acids contained peptidoglycan with l-lysine as the diagnostic diamino acid. Strain JC303T contained MK-7 as the predominant (96 %) menaquinone with the presence of a significant amount (4 %) of MK-8. The DNA G+C content was 43 mol%. On the basis of morphological, physiological, genotypic, phylogenetic and chemotaxonomic analyses, strain JC303T is considered to represent a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus alkaliphilus sp. nov. is proposed. The type strain is JC303T (=KCTC 33662T=LMG 28756T). In addition, we propose to rename J. soli (Chen et al., 2010), an illegitimate homonym of the validly published name Jeotgalibacillus soli(Cunha et al., 2012) as Jeotgalibacillus terrae sp. nov. with type strain JSM 081008T (=DSM 22174T=KCTC 13528T).
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; India; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; Planococcaceae; RNA, Ribosomal, 16S; Salinity; Sequence Analysis, DNA; Sodium Chloride; Vitamin K 2
PubMed: 27667642
DOI: 10.1099/ijsem.0.001491 -
Journal of Biotechnology Jun 2015Jeotgalibacillus spp. are halophilic bacteria within the family Planococcaceae. No genomes of Jeotgalibacillus spp. have been reported to date, and their metabolic...
Jeotgalibacillus spp. are halophilic bacteria within the family Planococcaceae. No genomes of Jeotgalibacillus spp. have been reported to date, and their metabolic pathways are unknown. How the bacteria survive in hypertonic conditions such as seawater is yet to be discovered. As only few studies have been conducted on Jeotgalibacillus spp., potential applications of these bacteria are unknown. Here, we present the complete genome of J. malaysiensis D5(T) (=DSM 28777(T) =KCTC 33350(T)), which is invaluable in identifying interesting applications for this genus.
Topics: Adaptation, Biological; Base Composition; Base Sequence; Genome, Bacterial; Molecular Sequence Data; Planococcaceae; Plasmids; Salinity; Sequence Analysis, DNA
PubMed: 25858153
DOI: 10.1016/j.jbiotec.2015.03.007 -
International Journal of Systematic and... Nov 2001A moderately halophilic, round-endospore-forming bacterium (strain YKJ-13T) was isolated from jeotgal, a traditional Korean fermented seafood, and studied by a...
Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with L-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 . as mMrinibacillus marinus gen nov., comb. nov.
A moderately halophilic, round-endospore-forming bacterium (strain YKJ-13T) was isolated from jeotgal, a traditional Korean fermented seafood, and studied by a polyphasic taxonomic approach. This organism was related to the phylogenetic clade comprising members of Bacillus rRNA group 2 and formed a cluster with Bacillus marinus with a bootstrap fidelity value of 93.6%. The peptidoglycan type was A1alpha linked directly through L-Lys. Based on cell morphology, peptidoglycan type and phylogeny, strain YKJ-13T, together with B. marinus, is considered to be a member of Bacillus rRNA group 2. Strain YKJ-13T was also characterized by having MK-7 and MK-8 as the predominant menaquinones and iso-C15:0 as the major fatty acid. The DNA G+C content was 44 mol%. Strain YKJ-13T exhibited a 16S rDNA similarity value of 95.7% with B. marinus DSM 1297T, its closest phylogenetic relative. Levels of 16S rDNA similarity between strain YKJ-13T and other Bacillus spp. were less than 94.2%. Therefore, on the basis of the data presented, the name Jeotgalibacillus alimentarius gen. nov., sp. nov. is proposed for strain YKJ-13T (= KCCM 80002T = JCM 10872T). It is also proposed that B. marinus be reclassified in Marinibacillus gen. nov. as Marinibacillus marinus comb. nov.
Topics: Bacillus; Culture Media; DNA, Ribosomal; Fermentation; Korea; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Seafood; Sequence Analysis, DNA
PubMed: 11760951
DOI: 10.1099/00207713-51-6-2087 -
International Journal of Systematic and... Jul 2015A Gram-stain-positive, endospore-forming, rod-shaped bacterial strain, designated D5(T), was isolated from seawater collected from a sandy beach in a southern state of...
A Gram-stain-positive, endospore-forming, rod-shaped bacterial strain, designated D5(T), was isolated from seawater collected from a sandy beach in a southern state of Malaysia and subjected to a polyphasic taxonomic study. Sequence analysis of the 16S rRNA gene demonstrated that this isolate belongs to the genus Jeotgalibacillus, with 99.87% similarity to Jeotgalibacillus alimentarius JCM 10872(T). DNA-DNA hybridization of strain D5(T) with J. alimentarius JCM 10872(T) demonstrated 26.3% relatedness. The peptidoglycan type was A1α linked directly to L-lysine as the diamino acid. The predominant quinones identified in strain D5(T) were menaquinones MK-7 and MK-8.The major fatty acids were iso-C15:0 and anteiso-C15:0. The G+C content of its DNA was 43.0 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and sulfoquinovosyl diacylglycerol, as well as two unknown phospholipids and three unknown lipids. The phenotypic, chemotaxonomic and genotypic data indicated that strain D5(T) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus malaysiensis sp. nov. is proposed (type strain D5(T) = DSM 28777(T) = KCTC33550(T)). An emended description of the genus Jeotgalibacillus is also provided.
Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Malaysia; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan; Phospholipids; Phylogeny; Pigmentation; Planococcaceae; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Vitamin K 2
PubMed: 25862385
DOI: 10.1099/ijs.0.000242 -
International Journal of Systematic and... Jan 2010A Gram-variable, motile and rod-shaped bacterial strain, ASL-1(T), was isolated from a marine saltern located on the coast of the Yellow Sea, Korea. A neighbour-joining...
Jeotgalibacillus salarius sp. nov., isolated from a marine saltern, and reclassification of Marinibacillus marinus and Marinibacillus campisalis as Jeotgalibacillus marinus comb. nov. and Jeotgalibacillus campisalis comb. nov., respectively.
A Gram-variable, motile and rod-shaped bacterial strain, ASL-1(T), was isolated from a marine saltern located on the coast of the Yellow Sea, Korea. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain ASL-1(T) clustered with Jeotgalibacillus alimentarius YKJ-13(T) and that this cluster joined the clade comprising the type strains of two Marinibacillus species. Strain ASL-1(T) exhibited 16S rRNA gene sequence similarity values of 97.3 % to J. alimentarius YKJ-13(T) and 96.5 % to the type strains of Marinibacillus marinus and Marinibacillus campisalis. The chemotaxonomic properties of strain ASL-1(T) were similar to those of one or two of the genera Jeotgalibacillus and Marinibacillus. The peptidoglycan type was A1alpha linked directly through L-lysine as the diamino acid. Strain ASL-1(T) contained MK-7 as the predominant menaquinone with the presence of a significant amount of MK-8. The predominant fatty acid was anteiso-C(15 : 0). The DNA G+C content was 42.9 mol%. Differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain ASL-1(T) could be differentiated from J. alimentarius and the two Marinibacillus species. On the basis of the data presented, strain ASL-1(T) represents a novel species within the genus Jeotgalibacillus, for which the name Jeotgalibacillus salarius sp. nov. is proposed. The type strain is ASL-1(T) (=KCTC 13257(T)=CCUG 56751(T)). It is also proposed that Marinibacillus marinus and Marinibacillus campisalis be reclassified as Jeotgalibacillus marinus comb. nov. (type strain 581(T)=DSM 1297(T)=ATCC 29841(T)=CCUG 28884(T)=CIP 103308(T)=LMG 6930(T)) and Jeotgalibacillus campisalis comb. nov. (type strain SF-57(T)=KCCM 41644(T)=JCM 11810(T)), respectively.
Topics: Bacillales; Base Composition; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Geologic Sediments; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Seawater
PubMed: 19643870
DOI: 10.1099/ijs.0.008318-0 -
Antonie Van Leeuwenhoek Oct 2010A Gram-stain-positive, endospore-forming, motile, catalase- and oxidase-positive, aerobic, rod-shaped bacterium, designated strain JSM 081008(T), was isolated from...
A Gram-stain-positive, endospore-forming, motile, catalase- and oxidase-positive, aerobic, rod-shaped bacterium, designated strain JSM 081008(T), was isolated from non-saline forest soil in China. Strain JSM 081008(T) was able to grow with 0-20% (w/v) NaCl, at pH 6.0-10.5 and at 10-45 degrees C; optimum growth was observed with 2-5% (w/v) NaCl, at pH 7.0-8.0 and at 30-35 degrees C. The peptidoglycan type was A1alpha linked directly through L-Lys. The major cellular fatty acids (>10% of the total) were anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:0. The predominant respiratory quinone was menaquinone 7 and the genomic DNA G + C content of the strain was 42.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 081008(T) should be assigned to the genus Jeotgalibacillus and was related most closely to the type strains of Jeotgalibacillus alimentarius (sequence similarity 99.4%) and Jeotgalibacillus salarius (97.0%), followed by Jeotgalibacillus campisalis (95.4%) and Jeotgalibacillus marinus (95.2%). The combination of phylogenetic analysis, DNA-DNA relatedness values, phenotypic characteristics and chemotaxonomic data supports the view that strain JSM 081008(T) represents a novel species of the genus Jeotgalibacillus, for which the name Jeotgalibacillus soli sp. nov. is proposed. The type strain is JSM 081008(T) (=DSM 22174(T) = KCTC 13528(T)). An emended description of the genus Jeotgalibacillus is also presented.
Topics: Aerobiosis; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; DNA, Ribosomal; Geologic Sediments; Hydrogen-Ion Concentration; Locomotion; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; Planococcaceae; RNA, Ribosomal, 16S; Salinity; Sequence Analysis, DNA; Sodium Chloride; Soil Microbiology; Temperature; Trees
PubMed: 20490920
DOI: 10.1007/s10482-010-9455-z