-
Biochemical and Biophysical Research... Jan 2018It was earlier shown that the calcium load of rat liver mitochondria in medium containing TlNO and KNO resulted in the Tl-induced mitochondrial permeability transition...
It was earlier shown that the calcium load of rat liver mitochondria in medium containing TlNO and KNO resulted in the Tl-induced mitochondrial permeability transition pore (MPTP) opening in the inner membrane. This opening was accompanied by an increase in swelling and membrane potential dissipation and a decrease in state 3, state 4, and 2,4-dinitrophenol-uncoupled respiration. This respiratory decrease was markedly leveled by mersalyl (MSL), the phosphate symporter (PiC) inhibitor which poorly stimulated the calcium-induced swelling, but further increased the potential dissipation. All of these effects of Ca and MSL were visibly reduced in the presence of the MPTP inhibitors (ADP, N-ethylmaleimide, and cyclosporine A). High MSL concentrations attenuated the ability of ADP to inhibit the MPTP. Our data suggest that the PiC can participate in the Tl-induced MPTP opening in the inner membrane of Ca-loaded rat liver mitochondria.
Topics: Animals; Calcium; In Vitro Techniques; Ion Transport; Membrane Potential, Mitochondrial; Mersalyl; Mitochondria, Liver; Mitochondrial Membrane Transport Proteins; Mitochondrial Membranes; Mitochondrial Permeability Transition Pore; Mitochondrial Swelling; Oxygen Consumption; Rats; Rats, Wistar; Thallium
PubMed: 29223393
DOI: 10.1016/j.bbrc.2017.12.023 -
Antimicrobial Agents and Chemotherapy Sep 1975Mersalyl (Salyrgan), an organic mercurial diuretic, was tested against human and animal viruses with in vivo model infections in mice and tissue culture systems....
Mersalyl (Salyrgan), an organic mercurial diuretic, was tested against human and animal viruses with in vivo model infections in mice and tissue culture systems. Mersalyl was active against coxsackieviruses A21 and B1 in mice if administered intraperitoneally immediately after infection. No effect was observed if intraperitoneal treatment was delayed 1 or 2 h postinfection, or if treatment was administered either subcutaneously or per os. Topical treatment with a 5% aqueous solution of mersalyl produced a statistically significant effect against herpes simplex dermatitis in mice but the substance was inactive against systemic infections in mice with herpes simplex as well as Columbia SK, influenza, Semliki Forest, and Sendai viruses. Contact inactivation of coxsackieviruses A21 and B1 and herpes simplex virus was observed, but mersalyl was inactive in tissue culture against coxackieviruses A21 and B1, herpes simplex, influenza, rhinovirus, Semliki Forest, Sendai, and vaccinia viruses.
Topics: Animals; Antiviral Agents; Cells, Cultured; Haplorhini; Macaca mulatta; Mersalyl; Mice; Organomercury Compounds; Virus Diseases
PubMed: 810082
DOI: 10.1128/AAC.8.3.295 -
Molecular Pharmacology Nov 1998In response to hypoxia, mammalian cells express multiple gene products [including erythropoietin (EPO) and vascular endothelial growth factor (VEGF)] that serve to...
In response to hypoxia, mammalian cells express multiple gene products [including erythropoietin (EPO) and vascular endothelial growth factor (VEGF)] that serve to increase O2 delivery, as well as glucose transporters and glycolytic enzymes (such as enolase 1) that allow metabolic adaptation to decreased O2 availability. Increased transcription of the genes encoding these proteins in hypoxic cells is mediated by hypoxia-inducible factor 1 (HIF-1), a basic helix-loop-helix transcription factor. Expression of HIF-1 and downstream genes can also be induced by exposure of cells to divalent metals (such as CoCl2) or iron chelators [such as desferrioxamine (DFO)]. We report here that the organomercurial compound mersalyl induced expression of VEGF and enolase 1 mRNA, as well as HIF-1 activity, in cultured cells. Expression of reporter genes containing hypoxia response elements from the EPO and VEGF genes was also induced by mersalyl treatment. However, mersalyl inhibited endogenous EPO mRNA expression induced by hypoxia, CoCl2, or DFO. In cells lacking expression of the insulin-like growth factor-1 receptor, mersalyl did not induce HIF-1 activity or VEGF mRNA expression, whereas induction by hypoxia, CoCl2, or DFO was unaffected. The mitogen-activated protein kinase kinase inhibitor PD098059 markedly reduced induction of HIF-1 by mersalyl but not by hypoxia. These results indicate that mersalyl induces expression of HIF-1 and a subset of hypoxia-inducible genes by a mechanism, involving the insulin-like growth factor-1 receptor and mitogen-activated protein kinase activity, that is distinct from mechanisms of induction by hypoxia, CoCl2, or DFO.
Topics: Animals; Antimutagenic Agents; Base Sequence; Cell Hypoxia; Chelating Agents; Cobalt; DNA; DNA-Binding Proteins; Deferoxamine; Endothelial Growth Factors; Enzyme Inhibitors; Erythropoietin; Fibroblasts; Gene Expression Regulation, Neoplastic; Hepatoblastoma; Humans; Hypoxia-Inducible Factor 1; Hypoxia-Inducible Factor 1, alpha Subunit; Liver Neoplasms; Lymphokines; Mersalyl; Nuclear Proteins; Phosphopyruvate Hydratase; RNA, Messenger; Rats; Receptor, IGF Type 1; Transcription Factors; Tumor Cells, Cultured; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
PubMed: 9804609
DOI: 10.1124/mol.54.5.749 -
Canadian Medical Association Journal Aug 1956
Topics: Diuretics; Drug Overdose; Mersalyl; Organomercury Compounds; Poisoning
PubMed: 13343095
DOI: No ID Found -
Current Medical Research and Opinion 1974
Topics: Acute Disease; Anticoagulants; Antihypertensive Agents; Arteritis; Arthritis; Barbiturates; Contraceptives, Oral; Digitalis Glycosides; Drug-Related Side Effects and Adverse Reactions; Edema; Gout; Humans; Hydralazine; Iatrogenic Disease; Lupus Erythematosus, Systemic; Mersalyl; Osteomalacia; Osteoporosis; Penicillins; Procainamide; Pyrazinamide; Succinylcholine; Sulfonamides; Water-Electrolyte Balance
PubMed: 4452289
DOI: 10.1185/03007997409115250 -
Biochimica Et Biophysica Acta Feb 1986We have examined the effect of a mercurial sulfhydryl reagent, mersalyl, on the protein composition of cytoskeletons by SDS-polyacrylamide gel electrophoresis after...
We have examined the effect of a mercurial sulfhydryl reagent, mersalyl, on the protein composition of cytoskeletons by SDS-polyacrylamide gel electrophoresis after treatment of human platelets with Triton X-100 (Triton) containing mersalyl and Ca2+, and have found that mersalyl alters the protein composition of cytoskeletons in a Ca2+-dependent manner. At 1 X 10(-7) M Ca2+, 0.2 mM mersalyl, which represents approximately the equivalent amount of sulfhydryl of platelet suspensions that we used, specifically made myosin insoluble. The amount of myosin in Triton-mersalyl residues was increased by increasing the Ca2+ concentration of Triton lysis buffer. Actin-binding protein, 235 kDa polypeptide and alpha-actinin-like protein were decreased in Triton residues by mersalyl at Ca2+ concentrations less than 1 X 10(-7) M, while these polypeptides in Triton residues were increased by mersalyl in the presence of more than 2 X 10(-7) M Ca2+. Electron microscopic study revealed the presence of thick filaments with an appearance similar to that of the thick filaments of platelet myosin. Thus, the modification with mersalyl of sulfhydryls of platelet polypeptides along with changes in Ca2+ concentrations within a physiological range leads to changes in solubility of, and filament formation of, myosin, actin and other cytoskeletal proteins.
Topics: Actomyosin; Adenosine Triphosphatases; Blood Platelets; Calcium; Chemical Precipitation; Cytoskeletal Proteins; Electrophoresis, Polyacrylamide Gel; Humans; Mersalyl; Microscopy, Electron; Myosins; Octoxynol; Organomercury Compounds; Polyethylene Glycols; Solubility; Sulfhydryl Compounds; Sulfhydryl Reagents
PubMed: 2935197
DOI: 10.1016/0304-4165(86)90080-2 -
The Journal of Pharmacology and... Mar 1955
Topics: Body Fluids; Diuretics; Mersalyl; Organomercury Compounds; Polarography; Sulfhydryl Compounds; Urine
PubMed: 14368491
DOI: No ID Found -
Hoppe-Seyler's Zeitschrift Fur... Dec 1981Pig heart mitochondria were incubated with [203Hg]mersalyl and the radioactive pattern was analyzed by fluorography after dodecyl sulfate gel electrophoresis. No...
Pig heart mitochondria were incubated with [203Hg]mersalyl and the radioactive pattern was analyzed by fluorography after dodecyl sulfate gel electrophoresis. No differences in the radioactivity distribution were found after labeling with various mersalyl concentrations, at different pH and after labeling in the native or dodecyl sulfate-dissociated state of mitochondria. A redistribution of [203Hg]mersalyl between various proteins in the presence of dodecyl sulfate could directly be demonstrated by mixing labeled membranes with unlabeled matrix proteins, as well as by comparison of the radioactivity patterns of whole mitochondria labeled with irreversibly reacting N-([2-3H]ethyl)maleimide and reversibly binding [203Hg]mersalyl. From these data it is concluded that under native conditions mersalyl is principally bound to the phosphate carrier protein, whereas during dissociation in dodecyl sulfate the organomercurial is redistributed and mainly attached to the ADP/ATP-carrier protein.
Topics: Animals; Carrier Proteins; Mersalyl; Mitochondria, Heart; Organomercury Compounds; Phosphate-Binding Proteins; Phosphates; Protein Denaturation; Swine
PubMed: 7319473
DOI: 10.1515/bchm2.1981.362.2.1583 -
The Journal of Pharmacy and Pharmacology Jun 1949
Topics: Biological Assay; Humans; Mersalyl
PubMed: 18145474
DOI: 10.1111/j.2042-7158.1949.tb12439.x -
British Journal of Pharmacology and... Sep 1946
Topics: Animals; Bronchodilator Agents; Diuresis; Kidney; Mercury; Mercury Compounds; Mersalyl; Rats; Sodium Acetate; Theophylline
PubMed: 19108088
DOI: 10.1111/j.1476-5381.1946.tb00038.x