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The Journal of Biological Chemistry Nov 1977
Topics: Animals; Cattle; Energy Metabolism; Hydrogen-Ion Concentration; Mersalyl; Mitochondria; Mitochondria, Heart; Mitochondrial Swelling; Nigericin; Nitrates; Oxygen Consumption; Potassium; Sodium; Succinates
PubMed: 21185
DOI: No ID Found -
Acta Physiologica Scandinavica May 1960
Topics: Diuretics; Mersalyl; Muscle Contraction; Muscles; Organomercury Compounds; Zinc
PubMed: 13819340
DOI: 10.1111/j.1748-1716.1960.tb01932.x -
Experimental Eye Research Oct 1993Degradation of substance P was studied in dog and rabbit aqueous humor. Substance P inactivation was followed by the bioassay using the isolated guinea pig ileum. Both... (Comparative Study)
Comparative Study
Degradation of substance P was studied in dog and rabbit aqueous humor. Substance P inactivation was followed by the bioassay using the isolated guinea pig ileum. Both rabbit and dog aqueous humor inactivated substance P. Rabbit aqueous humor inactivated the peptide faster than dog aqueous humor. Inactivation of substance P by rabbit aqueous humor was inhibited by diisopropylfluorophosphate while other enzyme inhibitors tested (captopril, phosphoramidon, mersalyl acid and p-chloromercuriphenyl-sulphonate) were practically ineffective or had a partial inhibitory effect. Our results suggest that serine proteases, rather than other peptidases, play a major role in the inactivation of substance P in aqueous humor.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Aqueous Humor; Biological Assay; Dogs; Ileum; In Vitro Techniques; Isoflurophate; Muscle Contraction; Rabbits; Substance P
PubMed: 7506669
DOI: 10.1006/exer.1993.1142 -
Acta Physiologica Et Pharmacologica... 1955
Topics: Animals; Diuretics; Kidney; Mersalyl; Organomercury Compounds; Phosphates; Rats
PubMed: 14387732
DOI: No ID Found -
Indian Journal of Biochemistry &... Dec 1977
Comparative Study
Topics: Animals; Ethylmaleimide; Hydrogen-Ion Concentration; In Vitro Techniques; Mersalyl; Mitochondria, Liver; Organomercury Compounds; Potassium; Rats; Stimulation, Chemical
PubMed: 27451
DOI: No ID Found -
Current Eye Research Jul 1990The presence of the membrane transport mechanism, lactate-H+ cotransport, was tested in explants of rabbit corneal epithelium. Basal corneal epithelial cells were loaded...
The presence of the membrane transport mechanism, lactate-H+ cotransport, was tested in explants of rabbit corneal epithelium. Basal corneal epithelial cells were loaded with the pH sensitive fluorescent dye BCECF. Intracellular pH (pHi) was measured by rationing the fluorescence emission output following excitation at 490 and 440 nm. Perfusion of explants in lactate-containing Ringer's, pH 7.40, produced a reversible decrease in pHi. The lactate induced proton influx (mM/min) followed saturating kinetics, Km = 10.7 mM lactate, Vmax = 10.2 mM/min. Proton influx following addition of 10 mM lactate was inhibited 36, 60 and 47% by pre-perfusion in 1 mM CHC (cyano-hydroxycinammic acid), 500 microM H2DIDS (4,4'-diisothiocyanato-dihydrostilbene-2,2'-disulfonic acid) and 1 mM LAIE (lactic acid isobutylester), respectively. These inhibitors of lactate-H+ cotransport were reversible. Mersalyl acid (500 microM) inhibited proton flux from 10 mM lactate addition by nearly 100%, but was irreversible. Stimulation of lactate production by perfusion in N2 equilibrated Ringer's (hypoxia) or the addition of 1 mM NaCN led to a slow alkalinization (0.1 pH unit in 10 min). Pre-perfusion with the reversible inhibitors slowed the hypoxic alkalinization by approximately 40%. It is concluded that lactate-H+ cotransport is present in the corneal epithelium and that it contributes to pHi regulation during hypoxia.
Topics: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; Animals; Biological Transport; Calibration; Cornea; Coumaric Acids; Epithelium; Fluorescent Dyes; Fluorometry; Hydrogen-Ion Concentration; In Vitro Techniques; Lactates; Mersalyl; Perfusion; Protons; Rabbits
PubMed: 2170077
DOI: 10.3109/02713689008999587 -
Annual Review of Physiology 1976The renal diluting segment (thick ascending limb of Henle's loop) reabsorbs sodium chloride in excess of water and is responsible for dilution of the urine as well as... (Review)
Review
The renal diluting segment (thick ascending limb of Henle's loop) reabsorbs sodium chloride in excess of water and is responsible for dilution of the urine as well as reabsorption of a large fraction of the salt present in the glomerular ultrafiltrate. There is active reabsorption of chloride, which causes the voltage to be positive in the tubule lumen. Most, if not all, of the sodium transport is passive, driven by the voltage. Three major diuretics (mersalyl, furosemide, and ethacrynic acid) act in the lumen of the diluting segment to inhibit active chloride transport, not sodium transport as previously believed. This specific action on chloride transport may explain how these drugs are able to inhibit salt transport in the kidney while having so little effect on the transport processes elsewhere in the body.
Topics: Adenosine Triphosphatases; Animals; Biological Transport, Active; Chlorides; Diuretics; Dose-Response Relationship, Drug; Electrophysiology; Ethacrynic Acid; Furosemide; Kidney Tubules; Loop of Henle; Mersalyl; Permeability; Rabbits; Sodium; Sodium Chloride; Water
PubMed: 130827
DOI: 10.1146/annurev.ph.38.030176.000345 -
FEBS Letters Dec 1980
Comparative Study
Topics: Animals; Biological Transport, Active; Ethylmaleimide; Mersalyl; Mitochondria, Heart; Mitochondrial ADP, ATP Translocases; Muscle Proteins; Nucleotidyltransferases; Phosphates; Swine
PubMed: 6257545
DOI: 10.1016/0014-5793(80)80349-8 -
Acta Pharmacologica Et Toxicologica 1965
Topics: Blood; Body Fluids; Chlorides; Connective Tissue; Diuretics; Electrolytes; Estradiol; Hexosamines; Hydroxyproline; Mersalyl; Mice; Organomercury Compounds; Pharmacology; Potassium; Research; Skin; Sodium; Sodium, Dietary; Theophylline; Urine; Water-Electrolyte Balance
PubMed: 14254795
DOI: No ID Found -
Acta Pharmacologica Et Toxicologica 1958
Topics: Connective Tissue; Diuretics; Humans; Leadership; Mersalyl; Organomercury Compounds; Theophylline
PubMed: 13594499
DOI: 10.1111/j.1600-0773.1958.tb02981.x