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American Journal of Ophthalmology Nov 1984Blepharoconjunctivitis caused by methicillin-resistant strains of Staphylococcus epidermidis occurred in two hospitalized patients, a 74-year-old man and a 19-year-old...
Blepharoconjunctivitis caused by methicillin-resistant strains of Staphylococcus epidermidis occurred in two hospitalized patients, a 74-year-old man and a 19-year-old man. Both strains were resistant to multiple courses of topical antibiotic therapy. Successful treatment in both cases depended upon antibiotic sensitivity testing. These methicillin-resistant strains of S. epidermidis, like methicillin-resistant strains of Staphylococcus aureus, demonstrated almost complete cross-resistance to cephalosporins despite apparent sensitivity on Kirby-Bauer disk sensitivity testing. Topical vancomycin was curative in one case. In the other case, treatment with topical gentamicin, intravenous cefoperazone, and oral rifampin led to resolution of the symptoms.
Topics: Adult; Aged; Blepharitis; Eyelid Diseases; Humans; Male; Methicillin; Penicillin Resistance; Staphylococcal Infections; Staphylococcus epidermidis
PubMed: 6568091
DOI: 10.1016/0002-9394(84)90241-1 -
Lancet (London, England) Sep 1985
Topics: Humans; Methicillin; Penicillin Resistance; Staphylococcus aureus
PubMed: 2863609
DOI: No ID Found -
Singapore Medical Journal Oct 1987
Topics: Humans; Methicillin; Penicillin Resistance; Staphylococcus aureus
PubMed: 3433102
DOI: No ID Found -
The Medical Journal of Australia Feb 1984
Topics: Methicillin; Penicillin Resistance; Staphylococcus aureus
PubMed: 6559256
DOI: No ID Found -
The Medical Journal of Australia Oct 1981
Topics: Chronic Disease; Humans; Methicillin; Penicillin Resistance; Staphylococcus aureus
PubMed: 7321964
DOI: No ID Found -
Northwest Medicine Jan 1969
Topics: Methicillin; Penicillin Resistance; Staphylococcus
PubMed: 5189757
DOI: No ID Found -
The Indian Journal of Medical Research Sep 1982
Comparative Study
Topics: Humans; Methicillin; Penicillin G; Penicillin Resistance; Staphylococcus aureus
PubMed: 6924635
DOI: No ID Found -
Journal of Microbiology and... May 2023Rapid diagnosis of methicillin-resistant (MRSA) is essential for guiding clinical treatment and preventing the spread of MRSA infections. Herein, we present a simple...
Rapid diagnosis of methicillin-resistant (MRSA) is essential for guiding clinical treatment and preventing the spread of MRSA infections. Herein, we present a simple and rapid MRSA screening test based on the aggregation effect of mannose-binding lectin (MBL)-conjugated gold nanoparticles (AuNP), called the MRSA probe. Recombinant MBL protein is a member of the lectin family and part of the innate immune system. It can recognize wall teichoic acid (WTA) on the membrane of MRSA more specifically than that of methicillin-sensitive (MSSA) under optimized salt conditions. Thus, the MRSA probe can selectively bind to MRSA, and the aggregation of the probes on the surface of the target bacteria can be detected and analyzed by the naked eye within 5 min. To demonstrate the suitability of the method for real-world application, we tested 40 clinical isolates (including 20 MRSA specimens) and recorded a sensitivity of 100%. In conclusion, the MRSA probe-based screening test with its excellent sensitivity has the potential for successful application in the microbiology laboratory.
Topics: Humans; Methicillin-Resistant Staphylococcus aureus; Staphylococcus aureus; Gold; Metal Nanoparticles; Methicillin; Staphylococcal Infections; Anti-Bacterial Agents; Microbial Sensitivity Tests
PubMed: 36959167
DOI: 10.4014/jmb.2301.01004 -
BioMed Research International 2023Methicillin-resistant (MRSA) has evolved resistance even against the last resort -lactam antibiotics. This is because of the acquisition of an additional...
Methicillin-resistant (MRSA) has evolved resistance even against the last resort -lactam antibiotics. This is because of the acquisition of an additional penicillin-binding protein 2a (PBP2a) which is a resistance determinant in MRSA. Currently, available PBP2a inhibitors are ineffective against life-threatening and fatal infections caused by microorganisms. Therefore, there is an urgent need to screen natural compounds that could overpass the resistance issue alone or in combination with antibacterial drugs. We studied the interactions of different phytochemicals with PBP2a so that crosslinking of peptidoglycans could be inhibited. In structure-based drug designing, approach plays a key role in determining phytochemical interactions with PBP2a. In this study, a total of 284 antimicrobial phytochemicals were screened using the molecular docking approach. The binding affinity of methicillin, -11.241 kcal/mol, was used as the threshold value. The phytochemicals having binding affinities with PBP2a stronger than methicillin were identified, and the drug-likeness properties and toxicities of the screened phytochemicals were calculated. Out of the multiple phytochemicals screened, nine were found as good inhibitors to be PBP2a, among which cyanidin, tetrandrine, cyclomorusin, lipomycin, and morusin showed strong binding potential with the receptor protein. These best-selected phytochemicals were also docked to the allosteric site of PBP2a, and most of the compounds revealed strong interactions with the allosteric site. These compounds were safe to be used as drugs because they did not show any toxicity and had good bioactivity scores. Cyanidin had the highest binding affinity (S-score of -16.061 kcal/mol) with PBP2a and with high gastrointestinal (GI) absorption. Our findings suggest that cyanidin can be used as a drug against MRSA infection either in purified form or that its structure can lead to the development of more potent anti-MRSA medicines. However, experimental studies are required to evaluate the inhibitory potential of these phytochemicals against MRSA.
Topics: Methicillin-Resistant Staphylococcus aureus; Methicillin; Molecular Docking Simulation; Anti-Bacterial Agents; Penicillin-Binding Proteins; Phytochemicals; Bacterial Proteins; Microbial Sensitivity Tests
PubMed: 37250750
DOI: 10.1155/2023/5100400 -
European Journal of Clinical... Jul 1999
Topics: Cost-Benefit Analysis; Delivery of Health Care; Gene Frequency; Guidelines as Topic; Humans; Methicillin; Methicillin Resistance; Netherlands; Penicillins; Staphylococcal Infections; Staphylococcus aureus
PubMed: 10482021
DOI: 10.1007/s100960050324