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Microbiological Research Nov 2021Biofilm formation by pathogenic bacteria as well as their resilience to antibiotic treatments are a major health problem. Here, we sequenced and analyzed the genome of...
Biofilm formation by pathogenic bacteria as well as their resilience to antibiotic treatments are a major health problem. Here, we sequenced and analyzed the genome of the clinical methicillin-resistant Staphylococcus aureus S1 (MRSA-S1) strain and established its sensitivity to the combination of methicillin and the leaderless two peptides enterocin DD14 (EntDD14). Such sensitivity was assessed in vitro based on the MIC/FIC values as well as on killing curves experiments. Moreover, combination of EntDD14 and methicillin was able to reduce the biofilm formation of Staphylococcus aureus S1 of about ∼30 %. Interestingly, genes thought to be involved in the virulence of MRSA-S1, like nuc and pvl which code, respectively, for nuclease and Panton-Valentine leucocidin, were shown to be downregulated following treatment with EntDD14 and methicillin. Similar effects were registered for other genes such as cflA, cflB and icaB, coding for bacterial ligands clumping factors A, B and intercellular adhesion factor respectively. All these data, suggest that combinations of bacteriocins and antibiotics are useful as a backup for treatment of bacterial infections.
Topics: Bridged-Ring Compounds; Drug Resistance, Bacterial; Drug Synergism; Methicillin; Methicillin-Resistant Staphylococcus aureus
PubMed: 34521050
DOI: 10.1016/j.micres.2021.126864 -
Lancet (London, England) Feb 1974
Comparative Study
Topics: Animals; Cephalothin; Kanamycin; Methicillin; Penicillin Resistance; Rats; Staphylococcal Infections; Staphylococcus; Vancomycin
PubMed: 4130509
DOI: 10.1016/s0140-6736(74)92636-1 -
Lancet (London, England) Apr 1975
Topics: Drug and Narcotic Control; Fever; Humans; Methicillin; United States
PubMed: 48098
DOI: 10.1016/s0140-6736(75)93050-0 -
Cell Transplantation 2023is one of the most common organisms isolated from respiratory secretions in lung transplant donors and recipients perioperatively. Within the first 90 days after lung...
is one of the most common organisms isolated from respiratory secretions in lung transplant donors and recipients perioperatively. Within the first 90 days after lung transplantation, methicillin-susceptible (MSSA) infections have been associated with increased mortality and acute and chronic rejection. However, it is unclear whether respiratory cultures positive for MSSA at the time of transplantation can lead to clinically significant infection. The aim of this study was to assess the microbiological and clinical outcomes for lung transplant recipients (LTRs) with positive perioperative donor or/and recipient respiratory cultures for MSSA. A retrospective study was conducted evaluating MSSA-positive respiratory cultures at the time of lung transplantation from donors and/or recipients from January 1, 2008, to December 30, 2019. Patients who did not have a bronchoalveolar lavage at 2 weeks after the lung transplant or died within 2 weeks of lung transplant were excluded. The main outcome was MSSA eradication at 2-week bronchoscopy. Recipients were evaluated for MSSA infections at the 12-week period after the transplant. Of the 1,678 individuals who underwent lung transplantation, 218 LTRs had isolated in perioperative donor or recipient respiratory cultures, and 29 were subsequently excluded. Of the remaining 189 LTRs, MSSA eradication at the 2-week bronchoscopy was achieved in 186 (98.4%) recipients. During the 12-week follow-up, 15 (7.9%) recipients were diagnosed with MSSA pneumonia; concurrent MSSA bacteremia was noted in one recipient. No anastomotic infection, empyema, or lung abscess related to MSSA was diagnosed during the follow-up period.In LTRs, the rate of MSSA eradication at 2-week post-transplant recipients is high, and it is associated with a low rate of infectious complication within the first 12 weeks after transplant. Most of the recipients received a combination therapy with at least one agent active against MSSA. More studies to evaluate the optimal antimicrobial stewardship policies regarding the regimen and duration of antibiotic therapy for these patients are needed.
Topics: Humans; Staphylococcus aureus; Methicillin; Retrospective Studies; Lung Transplantation; Lung
PubMed: 37452563
DOI: 10.1177/09636897231182480 -
Frontiers in Cellular and Infection... 2023is one of the species with the greatest clinical importance and greatest impact on public health. In fact, methicillin-resistant (MRSA) is considered a pandemic...
is one of the species with the greatest clinical importance and greatest impact on public health. In fact, methicillin-resistant (MRSA) is considered a pandemic pathogen, being essential to develop effective medicines and combat its rapid spread. This study aimed to foster the translation of clinical research outcomes based on metallodrugs into clinical practice for the treatment of MRSA. Bearing in mind the promising anti-Gram-positive effect of the heteroscorpionate ligand 1,1'-(2-(4-isopropylphenyl)ethane-1,1-diyl)bis(3,5-dimethyl-1H-pyrazole) (2P), we propose the coordination of this compound to platinum as a clinical strategy with the ultimate aim of overcoming resistance in the treatment of MRSA. Therefore, the novel metallodrug 2P-Pt were synthetized, fully characterized and its antibacterial effect against the planktonic and biofilm state of evaluated. In this sense, three different strains of were studied, one collection strain of sensitive to methicillin and two clinical MRSA strains. To appraise the antibacterial activity, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) were determined. Moreover, successful outcomes on the development of biofilm in a wound-like medium were obtained. The mechanism of action for 2P-Pt was proposed by measuring the MIC and MBC with EDTA (cation mediated mechanism) and DMSO (exogenous oxidative stress mechanism). Moreover, to shed light on the plausible antistaphylococcal mechanism of this novel platinum agent, additional experiments using transmission electron microscopy were carried out. 2P-Pt inhibited the growth and eradicated the three strains evaluated in the planktonic state. Another point worth stressing is the inhibition in the growth of MRSA biofilm even in a wounded medium. The results of this work support this novel agent as a promising therapeutic alternative for preventing infections caused by MRSA.
Topics: Methicillin-Resistant Staphylococcus aureus; Staphylococcus aureus; Platinum; Anti-Bacterial Agents; Methicillin; Microbial Sensitivity Tests; Biofilms
PubMed: 37051297
DOI: 10.3389/fcimb.2023.1100947 -
Journal of Clinical Microbiology Feb 1987
Topics: Methicillin; Penicillin Resistance; Staphylococcus aureus
PubMed: 3643929
DOI: 10.1128/jcm.25.2.463-464.1987 -
Infection Control : IC May 1987
Topics: Cross Infection; Humans; Methicillin; Penicillin Resistance; Staphylococcal Infections; Staphylococcus aureus
PubMed: 3647938
DOI: 10.1017/s0195941700065917 -
Changgeng Yi Xue Za Zhi Sep 1987
Topics: Methicillin; Microbial Sensitivity Tests; Oxacillin; Penicillin Resistance; Staphylococcus aureus; Vancomycin
PubMed: 3455291
DOI: No ID Found -
Zentralblatt Fur Bakteriologie,... Feb 1966
Topics: Culture Media; Methicillin; Penicillin Resistance; Staphylococcus; Staphylococcus Phages
PubMed: 4228505
DOI: No ID Found -
Lancet (London, England) Dec 1978
Topics: Acute Kidney Injury; Drug Synergism; Drug Therapy, Combination; Gentamicins; Humans; Methicillin
PubMed: 82822
DOI: 10.1016/s0140-6736(78)92082-2