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Analytical Biochemistry Jul 2020Methyl green (MG), a conventional, low-cost histological stain, was used to design a flow cytometric cell-cycle/DNA-ploidy assay. On fluorometry, MG absorbed maximally...
Methyl green (MG), a conventional, low-cost histological stain, was used to design a flow cytometric cell-cycle/DNA-ploidy assay. On fluorometry, MG absorbed maximally at 633-nm, showed negligible fluorescence in free-state, but emitted brightly when bound to DNA. Optimal dye and cell concentrations for staining and effects of time and photobleaching on stained cells were determined for a lyse-permeabilize-stain protocol. Linearity of DNA-binding, coefficients-of-variation of G0/G1-peaks and minimal carryover were confirmed. Assay results correlated highly with a propidium iodide-based kit in 29 acute lymphoblastic leukemia specimens. The MG-based DNA-ploidy assay represented an accurate and inexpensive alternative to conventional PI-based assays.
Topics: Cell Cycle; DNA, Neoplasm; Flow Cytometry; Fluorescent Dyes; Humans; Methyl Green; Neoplasms; Optical Imaging
PubMed: 32450059
DOI: 10.1016/j.ab.2020.113782 -
Stain Technology Sep 1952
Topics: Coloring Agents; Staining and Labeling
PubMed: 12995174
DOI: 10.3109/10520295209105083 -
The Journal of Medical Laboratory... Oct 1967
Topics: Amyloid; Gentian Violet; Microtomy; Staining and Labeling
PubMed: 4167845
DOI: No ID Found -
The Journal of General Physiology Jan 1950Study of the stoichiometry of the DNA-methyl green reaction by dialysis, precipitation of stain-nucleic acid mixtures, and the staining of nuclei of known DNA content,...
Study of the stoichiometry of the DNA-methyl green reaction by dialysis, precipitation of stain-nucleic acid mixtures, and the staining of nuclei of known DNA content, indicate that the compound consists of one dye molecule per 10 P. The significance of this result was discussed in the preceding paper (1). Histone and lanthanum (and probably other multivalent cations (3)) compete with the dye for the nucleic acid molecule, indicating a common site of attachment, presumably the phosphoric acid groups. With care in the avoidance of procedures which might depolymerize DNA, and the use of a buffer at about pH 4.1, a quantitative histochemical method for DNA by the use of methyl green is possible. Pyronin staining appears to be of qualitative significance only. Slight differences in degree of polymerization, as between the shad and mammalian DNA appear to have no effect on methyl green staining. It may be that a critical level of polymerization for DNA staining exists. This level must exceed 20 nucleotides to account for the 10 P to 1 dye molecule and the effect on the methyl green absorption spectrum; but it may be considerably greater. Beyond this critical level, whatever it may be, further polymerization probably has no influence on staining.
Topics: Animals; Buffers; DNA; Methyl Green; Nucleic Acids; Nucleotides; Phosphoric Acids; Pyronine; Staining and Labeling
PubMed: 15409512
DOI: 10.1085/jgp.33.3.265 -
The Journal of General Physiology Jan 19501. Methyl green stains selectively highly polymerized desoxyribonucleic acid, and fails to stain, to any significant extent, depolymerized desoxyribonucleic acid and...
1. Methyl green stains selectively highly polymerized desoxyribonucleic acid, and fails to stain, to any significant extent, depolymerized desoxyribonucleic acid and ribonucleic acid. 2. Pyronin stains preferentially low polymers of nucleic acid. 3. Triphenylmethane dyes with two amino groups appear to share the selectivity of methyl green. Those with three amino groups are not selective. 4. A stereochemical hypothesis is offered to account for these observations.
Topics: Coloring Agents; Methyl Green; Nucleic Acids; Pyronine; Staining and Labeling
PubMed: 15402708
DOI: 10.1085/jgp.33.3.243 -
International Journal of Biological... Feb 2022Modified chitosan with various functional groups has high potential as an efficient adsorbent in removing water pollution. In this study, new magnetic adsorbent,...
Modified chitosan with various functional groups has high potential as an efficient adsorbent in removing water pollution. In this study, new magnetic adsorbent, bio-based chitosan/FeO/NiFeO, was successfully prepared by green chemistry route involving mixing of chitosan as core moiety and FeO/NiFeO nanocomposite, and slow evaporation of solvent. Synthesized chitosan/FeO/NiFeO was characterized by FT-IR, TGA, XRD, VSM and FE-SEM. The FT-IR and XRD results confirmed that the successful preparation of chitosan/FeO/NiFeO. Uniform dispersion of FeO/NiFeO nanoparticles with low aggregation was confirmed by FE-SEM. The as-prepared magnetic chitosan/FeO/NiFeO was developed as solid phase adsorbent to remove methyl green (MG) dye from aqueous solutions. Several important parameters such as contact time, pH, temperature and adsorbent dosage were investigated systematically. The high and fast MG dye removal (≈ 80%) occurs after 30 min. The optimal conditions for MG removal was recorded at pH = 8, contact time of 60 min, adsorbent dosage of 0.2 g and 25 °C and displayed a high MG dye removal percentage of 96.51% and adsorption capacity of 77.22 mg/g.
Topics: Chitosan
PubMed: 34968538
DOI: 10.1016/j.ijbiomac.2021.12.082 -
FEBS Letters Jan 1993Interaction and binding geometries of complexes of Methyl green with poly(dA-dT)2, poly(dA).poly(dT), and triplex poly(dA).2poly(dT) complexes have been studied by...
Interaction and binding geometries of complexes of Methyl green with poly(dA-dT)2, poly(dA).poly(dT), and triplex poly(dA).2poly(dT) complexes have been studied by linear dichroism. For both of the complexes with double helical DNAs, the z symmetry axis of Methyl green is found to be approximately parallel to the DNA bases while the x symmetry axis lies at 40-44 degrees relative to the local DNA helix axis, in agreement with a groove binding mode. However, in contrast to minor-groove binders (such as DAPI and Hoechst 33258) Methyl green is found to be excluded from binding to the triple helical poly(dA).2poly(dT) in which the major groove is filled by the third strand. While most so far studied groove-binding dyes bind in the minor groove of DNA, Methyl green thus appears to be an exception.
Topics: Binding Sites; Circular Dichroism; DNA; Hydrogen Bonding; Methyl Green; Nucleic Acid Conformation; Spectrum Analysis
PubMed: 8416812
DOI: 10.1016/0014-5793(93)81133-k -
Stain Technology Nov 1966
Topics: Autoradiography; Cell Nucleolus; Cell Nucleus; Cell Wall; Chromosomes; Cytoplasm; DNA; Photography; Plant Cells; Pyrans; Staining and Labeling
PubMed: 4165878
DOI: 10.3109/10520296609116331 -
The Journal of General Physiology Nov 19501. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to...
1. Methyl green ("ethyl green") C. I. Number 685 was examined and found to behave identically with methyl green C. I. Number 684 (no longer available) in respect to molar extinction coefficient, effect of combination with polymerized DNA, failure to react with depolymerized DNA, and effect of pH. 2. The mass law permits the calculation of P/dye. This is found to be 13 P/dye. The same value is obtained when an excess of methyl green is caused to fade by adjusting the pH to 7.5. 3. The compound formed by methyl green with DNA has the same maximum absorption at 642.5 to 645 mmicro in the pH range 3.5-7.8, whereas the free dye fades markedly above pH 5.0.
Topics: DNA; Methyl Green; Nucleic Acids
PubMed: 14824487
DOI: 10.1085/jgp.34.2.147 -
The Histochemical Journal 1986Fully standardized Methyl Green-Pyronin methods are presented. Pure Pyronin Y and purified Methyl Green or Ethyl Green are used either simultaneously in one dye bath or...
Fully standardized Methyl Green-Pyronin methods are presented. Pure Pyronin Y and purified Methyl Green or Ethyl Green are used either simultaneously in one dye bath or are used as a sequence of Pyronin Y and Ethyl or Methyl Green. Both methods, as shown by enzymatic pretreatment, give a reliable and reproducible staining in DNA with Ethyl or Methyl Green and of RNA with Pyronin Y on Carnoy fixed material. On formaldehyde fixed material it was found advantageous to use the sequential method as chromatin was hereby stained green instead of blue as seen with the simultaneous method.
Topics: Animals; Buffers; DNA; Histocytochemistry; Methyl Green; Pyronine; RNA; Rats; Rosaniline Dyes; Staining and Labeling; Xanthenes
PubMed: 2426223
DOI: 10.1007/BF01675361