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Sheng Wu Gong Cheng Xue Bao = Chinese... Jul 2023The evaluation of the bioavailability of pollutants in soil is crucial to accurately assess the pollution risk, and whole-cell biosensor is one of the important tools...
The evaluation of the bioavailability of pollutants in soil is crucial to accurately assess the pollution risk, and whole-cell biosensor is one of the important tools for such evaluation. This study aimed to develop a novel whole-cell biosensor for the detection of methyl parathion in soil using. First, a whole-cell biosensor was constructed by the screened methyl parathion hydrolase gene, the existing specific induction element R, and the pUC19 plasmid skeleton. Then, the detection method of methyl parathion in soil extracts was established using 96-well microtiter plate as carrier and five whole-cell biosensors as indicator. The method was applied in the detection of methyl parathion in tested and field soil extracts. Taking . DH5α/pMP-AmilCP with the best detection performance as an example, this biosensor had a detection limit of 6.21-6.66 µg/L and a linear range of 10-10 000 µg/L for methyl parathion in four soil extracts. . DH5α/pMP-RFP and . DH5α/pMP-AmilCP methods have good detection performance for the analysis of methyl parathion in soil extract samples. This biosensor method can help to quickly assess the bioavailability of methyl parathion in soil, and thus help to understand the risk of soil pollution caused by organophosphorus pesticide methyl parathion.
Topics: Methyl Parathion; Pesticides; Organophosphorus Compounds; Escherichia coli; Soil; Farms; Biosensing Techniques
PubMed: 37584126
DOI: 10.13345/j.cjb.230129 -
Bioresource Technology Mar 2021Organophosphorus pesticides are highly toxic phosphate compounds with the general structure of O = P(OR) and threaten human health seriously. Methyl parathion...
In-depth biochemical identification of a novel methyl parathion hydrolase from Azohydromonas australica and its high effectiveness in the degradation of various organophosphorus pesticides.
Organophosphorus pesticides are highly toxic phosphate compounds with the general structure of O = P(OR) and threaten human health seriously. Methyl parathion hydrolase from microbial is an important enzyme to degrade organophosphorus pesticides (OPs) into less toxic or nontoxic compounds like. p-nitrophenol and diethyl phosphate. Here, a gene encoding methyl parathion hydrolase from Azohydromonas australica was firstly cloned and expressed in Escherichia coli. The recombinant hydrolase showed its optimal pH and temperature at pH 9.5 and 50 °C. Leveraging 1 mM Mn, the enzyme activity was significantly enhanced by 29.3-fold, and the thermostability at 40 and 50 °C was also improved. The recombinant MPH showed the specific activity of 4.94 and 16.0 U/mg towards methyl parathion and paraoxon, respectively. Moreover, A. australica MPH could effectively degrade various of OPs pesticides including methyl parathion, paraoxon, dichlorvos and chlorpyrifos in a few minutes, suggesting a great potential in the bioremediation of OPs pesticides.
Topics: Alcaligenaceae; Chlorpyrifos; Humans; Hydrolases; Methyl Parathion; Organophosphorus Compounds; Pesticides
PubMed: 33429316
DOI: 10.1016/j.biortech.2020.124641 -
International Journal of Environmental... Dec 2005Methyl parathion - MP (C[8]H[10rsqbNO[5rsqbPS) is a restricted-use pesticide that has been widely used as an agricultural insecticide. It belongs to the class of... (Review)
Review
Methyl parathion - MP (C[8]H[10rsqbNO[5rsqbPS) is a restricted-use pesticide that has been widely used as an agricultural insecticide. It belongs to the class of organophosphate chemicals characterized by their ability to inhibit acetylcholinesterase activity. The main route of human exposure is inhalation, but dermal contact and inadvertent ingestion can also be substantial. Populations that are susceptible to MP exposure primarily are applicators, manufacturers and individuals living near application and/or disposal sites. Exposure has also been reported as a result of illegal indoor application. MP related health effects include headaches, nausea, night-waking, diarrhea, difficulty breathing, excessive sweating and salivation, incoordination, and mental confusion. Other symptoms including behavior problems, motor skill problems and impairment of memory recall have also been reported. The primary targets of toxicity are the hematopoietic system (serum cholinesterase inhibition), the cardiovascular system (cardiovascular lesions, abnormalities in heart rate and increase in heart-to-body ratio), the reproductive system (placental morphology, fibrosis and hemorrhage, and inhibition of DNA synthesis in seminiferous tubules), and the nervous system (headache, muscle weakness, insomnia, dizziness, and impaired memory). MP is believed to not have any carcinogenic effects. In an attempt to update its toxicologic profile, we hereby provide a critical review of MP-related environmental and toxicologic effects, with a special emphasis on their potential implications for public health.
Topics: Animals; Cholinesterase Inhibitors; Ecotoxicology; Humans; Insecticides; Methyl Parathion
PubMed: 16819098
DOI: 10.3390/ijerph2005030007 -
Biosensors & Bioelectronics Oct 2018The electrochemical detection of methyl parathion in fish was performed by preconcentrating the pesticide on magnetic molecularly imprinted polymer and further readout...
The electrochemical detection of methyl parathion in fish was performed by preconcentrating the pesticide on magnetic molecularly imprinted polymer and further readout on magneto-actuated electrode by square wave voltammetry. The magnetic molecularly imprinted polymer was synthesized by a magnetic core-shell strategy, using methacrylic acid as a functional monomer, and selected by theoretical calculation using the density functional theory (DFT). The characterization of this material was performed by SEM, TEM and XRD. Moreover, the binding capacity and selectivity towards methyl parathion was studied and compared with the corresponding magnetic non-imprinted polymer. The magneto-actuated electrochemical sensor showed outstanding analytical performance for the detection of methyl parathion in fish, with a limit of detection of as low as 1.22 × 10 mg L and recovery values ranging from 89.4% to 94.7%. The magnetic molecularly imprinted polymer successfully preconcentrated the analyte from the complex samples and paves the way to incorporate this material in other platforms for the detection of this pesticide in the field of environmental control and food safety.
Topics: Animals; Electrochemical Techniques; Electrodes; Environmental Monitoring; Fishes; Food Analysis; Methyl Parathion; Molecular Imprinting; Polymers
PubMed: 30077132
DOI: 10.1016/j.bios.2018.06.052 -
Chemosphere Apr 2022The widespread use of methyl parathion (MP) and carbendazim (CBZ) as pesticide molecules for controlling pests and protect crops has added pollution issues; excess usage...
The widespread use of methyl parathion (MP) and carbendazim (CBZ) as pesticide molecules for controlling pests and protect crops has added pollution issues; excess usage of these can lead to atmospheric pollution through contaminating water and soil sources. In the present study, detection of these compounds at the trace level was achieved by employing graphene oxide (GO) and graphitic carbon nitride (g-CN) nanohybrid electrode assembly (GO/g-CN/GCE). The X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), and Atomic Force Microscopy (AFM) techniques were also used to characterize the materials developed to reveal their purity, crystal structure, and morphology. The complete voltammetric behavior of these analytes was investigated using cyclic voltammetic (CV) and square wave voltammetry (SWV) techniques. The influence of pH was studied and it was noticed that electrochemical response was the highest at pH 7.0 for MP and at pH 4.2 for CBZ. Density Functional Theory (DFT) calculations could help us to understand the adsorption behavior of MP and CBZ onto the GO and g-CN before their degradation due to the electrochemical reactions. SWV technique was helpful in the trace level detection of MP and CBZ. Linearity plots were obtained in the range of concentration from 8.0 × 10 M to 1.0 × 10 M with a limit of detection 0.824 nM for MP and 1.0 × 10 M to 2.5 × 10 M for CBZ with the detection limit of 2.82 nM. Significance of the developed method in the field of agricultural and environmental domains was successfully investigated by monitoring MP and CBZ in water and soil samples, and the obtained results suggested the selectivity, stability, and reproducibility of the newly developed GO/g-CN/GCE electrode assembly.
Topics: Benzimidazoles; Carbamates; Electrochemical Techniques; Electrodes; Graphite; Methyl Parathion; Nanocomposites; Nitriles; Reproducibility of Results
PubMed: 34979209
DOI: 10.1016/j.chemosphere.2021.133450 -
Journal of Environmental Sciences... 2009The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential...
The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonella typhimurium TA100 bacterial cells was observed without rat liver S9 metabolic activation. Parathion-methyl was further investigated for pathological changes in rat pancreas and liver. The long-term rat experiments showed that parathion-methyl exposure for 3 months can cause pathological changes in rat pancreases acinar cells and pancreatic hepatocytes. Atypical acinar cell focuses (AACF) were determined in the liver and pancreas of the rats. The results from short-term Ames test and long-term rat experiments suggested that parathion-methyl would be potential carcinogenic.
Topics: Animals; Biotransformation; Carcinogens; Insecticides; Liver; Liver Neoplasms, Experimental; Male; Methyl Parathion; Rats; Rats, Wistar; Salmonella typhimurium
PubMed: 20108674
DOI: 10.1016/s1001-0742(08)62326-8 -
Chemosphere Jun 2017Although the kinetics and transformation of methyl parathion have been investigated extensively, its abiotic degradation mechanism in anoxic sulfur-containing...
Although the kinetics and transformation of methyl parathion have been investigated extensively, its abiotic degradation mechanism in anoxic sulfur-containing groundwater system is still not clear. In this work, the abiotic degradation of methyl parathion in anoxic sulfur-containing system mediated by natural organic matter (NOM) was investigated in batch experiments. It was found that the removal of methyl parathion (up to 80.7%) was greatly improved in sulfide containing NOM compared to those in sulfide alone (with 15.5%) and in NOM alone (almost negligible). Various sulfur species presented significant differences in behaviors methyl parathion degradation, but followed by the pseudo-first-order model well. No facilitated degradation of methyl parathion was observed in sulfite (SO) or thiosulfate (SO) containing NOM such as anthraquinone. Although elemental sulfur (S) and cysteine could further improve the degradation rate of methyl parahtion, their impacts was very limited. The removal efficiency of methyl parathion in anoxic sulfur-containing system were related remarkably with NOM concentration and solution pH. Based on the transformation products identified by gas chromatography-mass spectrometer (GC/MS) and liquid chromatography high resolution mass spectrometer (LC/HRMS), both the nitro group reduction and hydrolysis (S@C) processes by sulfide (HS) were further proved to be two predominant reaction mechanisms for the abiotic degradation of methyl parathion in anoxic sulfur-containing system. The results of this study help to understand the natural attenuation of methyl parathion under anoxic sulfide-containing groundwater system mediated by NOM.
Topics: Gas Chromatography-Mass Spectrometry; Humic Substances; Hydrolysis; Kinetics; Methyl Parathion; Models, Theoretical; Solutions; Sulfides; Sulfites; Sulfur Compounds; Thiosulfates; Water Pollutants, Chemical
PubMed: 28273536
DOI: 10.1016/j.chemosphere.2017.02.109 -
Indian Journal of Physiology and... Apr 1992Sublethal doses of methyl parathion (O,O-dimethyl-O-nitrophenyl- thiophosphate) injected intraperitoneally to 15 and 21 day old rat pups induced regional alterations in...
Sublethal doses of methyl parathion (O,O-dimethyl-O-nitrophenyl- thiophosphate) injected intraperitoneally to 15 and 21 day old rat pups induced regional alterations in the central nervous system (CNS) in the levels of total RNA, total proteins, modulatory protein Calmodulin (CaM), in the activity levels of membrane bound enzyme Ca(2+)-ATPase and phospholipids. Levels of RNA and total proteins increased considerably in 15 days old methyl parathion treated (MPT) rat pups. Contrary to this the RNA and total protein content exhibited remarkable decrease in 21 day old methyl parathion treated animals. Calmodulin level showed an increase in cerebral cortex and brain stem and decrease in cerebellum and spinal cord in 15 day old methyl parathion treated rat pups. Whereas the level of Calmodulin decreased in cerebral cortex and cerebellum and increased in brain stem and spinal cord in 21 day old methyl parathion treated rat pups. Activity levels of calcium dependent ATPase showed significant inhibition in all the regions of Central Nervous System (CNS) of 15 and 21 day old methyl parathion treated rat pups. Phospholipids showed a general increase in all the regions of Central Nervous System on methyl parathion exposure. In the light of these observations, it has been suggested that the molecular regulatory mechanisms involving Ca2+/CaM are rendered inefficient due to toxic impact of methyl parathion.
Topics: Animals; Brain; Brain Stem; Calcium-Transporting ATPases; Calmodulin; Cerebellum; Cerebral Cortex; Electrophoresis, Polyacrylamide Gel; Injections, Intraperitoneal; Methyl Parathion; Phospholipids; Proteins; RNA; Rats; Spinal Cord
PubMed: 1380492
DOI: No ID Found -
Biosensors & Bioelectronics Jul 2011This article reports the fabrication of a nanocomposite biosensor for the sensitive and specific detection of methyl parathion. The nanocomposite sensing film was...
This article reports the fabrication of a nanocomposite biosensor for the sensitive and specific detection of methyl parathion. The nanocomposite sensing film was prepared via the formation of gold nanoparticles on silica particles, mixing with multiwall carbon nanotubes and subsequent covalent immobilization of methyl parathion hydrolase. The composite of the individual materials was finely tuned to offer the sensing film with high specific surface area and high conductivity. A significant synergistic effect of nanocomposites on the biosensor performance was observed in biosensing methyl parathion. The square wave voltammetric responses displayed well defined peaks, linearly proportional to the concentrations of methyl parathion in the range from 0.001 μg mL⁻¹ to 5.0 μg mL⁻¹ with a detection limit of 0.3 ng mL⁻¹. The application of this biosensor in the analysis of spiked garlic samples was also evaluated. The proposed protocol can be used as a platform for the simple and fast construction of biosensors with good performance for the determination of enzyme-specific electroactive species.
Topics: Biosensing Techniques; Electrochemical Techniques; Food Contamination; Garlic; Gold; Insecticides; Metal Nanoparticles; Methyl Parathion; Microscopy, Electron, Scanning; Nanocomposites; Phosphoric Monoester Hydrolases
PubMed: 21616655
DOI: 10.1016/j.bios.2011.04.025 -
Mikrochimica Acta May 2021A sensitive voltammetric sensor has been developed for hazardous methyl parathion detection (MP) using graphene oxide@Ce-doped TiO nanoparticle (GO@Ce-doped TiO NP)...
A sensitive voltammetric sensor has been developed for hazardous methyl parathion detection (MP) using graphene oxide@Ce-doped TiO nanoparticle (GO@Ce-doped TiO NP) electrocatalyst. The GO@Ce-doped TiO NPs were prepared through the sol-gel method and characterized by various physicochemical and electrochemical techniques. The GO@Ce-doped TiO NP-modified glassy carbon electrode (GCE) addresses excellent electrocatalytic activity towards MP detection for environmental safety and protection. The developed strategy of GO@Ce-doped TiO NPs at GCE surfaces for MP detection achieved excellent sensitivity (2.359 μA μM cm) and a low detection limit (LOD) 0.0016 μM with a wide linear range (0.002 to 48.327 μM). Moreover, the fabricated sensor shows high selectivity and long-term stability towards MP detection; this significant electrode further paves the way for real-time monitoring of environmental quantitative samples with satisfying recoveries.
Topics: Carbon; Catalysis; Cerium; Electrochemical Techniques; Electrodes; Graphite; Insecticides; Limit of Detection; Metal Nanoparticles; Methyl Parathion; Nanocomposites; Reproducibility of Results; Titanium
PubMed: 34052922
DOI: 10.1007/s00604-021-04847-5