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Foods (Basel, Switzerland) Dec 2023Interest in () is growing in the world of winemaking. is used both to protect musts from microbial spoilage and to modulate the aromatic profile of wines. Here, we...
Interest in () is growing in the world of winemaking. is used both to protect musts from microbial spoilage and to modulate the aromatic profile of wines. Here, we describe the isolation, characterization, and use of an autochthonous strain of in the vinification of Chasselas musts from the 2022 vintage. was used in co-fermentation with at both laboratory and experimental cellar scales. Our results showed that does not ferment sugars but has high metabolic activity, as detected by flow cytometry. Furthermore, sensory analysis showed that contributed slightly to the aromatic profile when compared to the control vinifications. The overall results suggest that our bioprospecting strategy can guide the selection of microorganisms that can be effectively used in the winemaking process.
PubMed: 38137289
DOI: 10.3390/foods12244485 -
Foods (Basel, Switzerland) Sep 2019One of the most important problems in the winemaking field is the increase of ethanol content in wine. Wines with high ethanol level negatively affect wine flavor and...
One of the most important problems in the winemaking field is the increase of ethanol content in wine. Wines with high ethanol level negatively affect wine flavor and human health. In this study, we evaluated the use of a selected strain of in immobilized form and under different aeration conditions, to reduce the ethanol content evaluating the volatile profile of the resulting wines. In a preliminary screening the best conditions regarding free/immobilized cells, static/aerated fermentation and inoculation level were identified. Bench-Top fermentation trials with different aeration conditions showed that the use of selected strain with aeration flow of 20 mL/L/min during the first 72 h of fermentation, led an ethanol reduction of 1.38% () in comparison with control strain. The analytical profile of the resulting wines did not show any negative feature. Indeed, the concentration of ethyl acetate, that above its sensory threshold impacts negatively the wine sensory profile, was found at an acceptable level. On the other hand, an increase in the concentration of significant fruity and flower compounds was found.
PubMed: 31480605
DOI: 10.3390/foods8090378 -
Current Microbiology Mar 2017Three yeast strains, named as FHL-A, FHL-B, and FHL-C, were isolated from peach fruit surfaces collected from different regions in the North of China highly produced...
Three yeast strains, named as FHL-A, FHL-B, and FHL-C, were isolated from peach fruit surfaces collected from different regions in the North of China highly produced protease and were presented as single separate group in the genus Metschnikowia by sequence comparisons of 26S rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region. BLASTn alignments on NCBI showed that the similarity of 26S rRNA gene sequences of the three strains to all sequences of other yeasts accessed into the GenBank/EMBL/DDBJ and other database was very low (≦93%). The phylogenetic tree based on the D1/D2 region of 26S rRNA gene sequences revealed that three strains are most closely related to Metschnikowia koreensis KCTC 7828T (AF257272.1) (sequence similarity: 93.0%) and Metschnikowia reukaufii CBS9709 (AJ716113.1) (sequence similarity: 93.0%). However, the strains are distinguished from M. koreensis by its non-assimilation of galactose, ribitol, and D-xylose, and by its growth at 37 °C or in vitamin-free medium, and are notably different from M. reukaufii by its non-assimilation of galactose, D-xylose, D-arabinose, and D-ribose, and by its growth at 35 °C or in vitamin-free medium. The strain FHL-B formed asci in V8 juice sporulation medium for 3 weeks. Therefore, the name Metschnikowia persici is proposed for the novel species, with FHL-B (= CBS12815 = CFCC 3578T) as the type strain.
Topics: Arabinose; China; DNA, Fungal; Endopeptidases; Galactose; Metschnikowia; Prunus persica; RNA, Ribosomal; Ribitol; Ribose; Xylose
PubMed: 28168603
DOI: 10.1007/s00284-017-1194-1 -
Biology Nov 2021is a non-conventional yeast with potential to be used in biotechnological processes, especially those involving low-cost feedstock exploitation and biocontrol...
is a non-conventional yeast with potential to be used in biotechnological processes, especially those involving low-cost feedstock exploitation and biocontrol applications. The combination of traits that supports these industrial applications in also makes it an attractive option to study in the context of livestock health. In this study, we examined the specific interactions between and multiple avian pathogenic bacteria. We tested individual bacteria-yeast interactions and bacterial combinations in both solid and liquid media and in variable nutrient environments. Across multiple isolates of , we observed different levels of antimicrobial activity, varying from supporting the growth of competing bacteria through suppression and bacterial killing, and we found that these responses varied depending on the bacterial strains and media. We identified multiple molecular routes, including proteins produced by strains, that acted to control these microbial interactions. Furthermore, protein screening revealed that strains were induced to produce proteins specifically when exposed to bacterial strains, suggesting that fine-tuned mechanisms allow to function as a potential lynchpin in a microbial community.
PubMed: 34943142
DOI: 10.3390/biology10121227 -
Toxins Jun 2021Wild yeasts isolated from the surface of apples were screened for antagonistic activity against , the main producer of the mycotoxin patulin. Three antagonistic yeasts...
Wild yeasts isolated from the surface of apples were screened for antagonistic activity against , the main producer of the mycotoxin patulin. Three antagonistic yeasts (Y33, Y29 and Y24) from a total of 90 were found to inhibit growth. Identification by ITS region sequence and characterization showed that three selected isolates of yeast should be different strains of Several concentrations of the selected yeasts were used to study their in vitro antifungal effectivity against on Petri dishes (plates with 63.6 cm surface) whereas their potential activity on patulin reduction was studied in liquid medium. Finally, the BCA that had the best in vitro antifungal capacity against and the best patulin degradation capacity was selected to be assessed directly on apples. All the selected strains demonstrated antifungal activity in vitro but the most efficient was the strain Y29. Isolated strains were able to reduce patulin content in liquid medium, Y29 being the only strain that completely reduced patulin levels within 120 h. The application of Y29 as biocontrol agent on the surface of apples inoculated with inhibited fungal growth and patulin production during storage. Therefore, the results shown that this yeast strain could be used for the reduction of and its mycotoxin in apples or apple-based products by adapting the procedure application.
Topics: Biological Control Agents; Fruit; Malus; Metschnikowia; Patulin; Penicillium; Plant Diseases
PubMed: 34199507
DOI: 10.3390/toxins13060397 -
Transboundary and Emerging Diseases Sep 2022The Chinese grass shrimp (Palaemonetes sinensis) was found with a white turbidity appearance in the Panjin area. After dissection, typical symptoms of milky disease with...
The Chinese grass shrimp (Palaemonetes sinensis) was found with a white turbidity appearance in the Panjin area. After dissection, typical symptoms of milky disease with hemolymph emulsification and noncoagulation were observed; however, the pathogen was unknown. In this study, we aimed to isolate the pathogen of the diseased P. sinensis. We found that the pathogen could grow on the fungal medium Bengal red, and microscopic examination showed that it reproduced by budding. Molecular identification of the isolated and purified yeast strain LNMB2021 based on 26S rDNA sequence showed that the pathogenic pathogen was Metschnikowia bicuspidata (GenBank OK094821), with 98.74% homology with M. bicuspidata strain LNES0119 (GenBank OK073903) and 98.56% with M. bicuspidata strain Liao (GenBank MT856369). The results of an artificial infection test showed that M. bicuspidata caused the same clinical symptoms in P. sinensis, and the isolated pathogen was still the same, which proved that P. sinensis was a new host of M. bicuspidata. Histopathological analysis showed that there were obvious pathological changes in the hepatopancreas and muscle tissue of the diseased P. sinensis. Identification of the pathogen is essential for the prevention and control of the disease and the healthy culture of P. sinensis. Furthermore, considering the transmissibility and cross-host transmission of M. bicuspidata, its risk of infecting other aquatic animals deserves high attention.
Topics: Animals; China; DNA, Ribosomal; Metschnikowia; Palaemonidae
PubMed: 35076183
DOI: 10.1111/tbed.14460 -
Antonie Van Leeuwenhoek 1977Ascosporulation in the yeast strain designated as Selenotila intestinalis Krassilnikov was achieved. On the basis of mode of ascus formation and ascospore morphology it...
Ascosporulation in the yeast strain designated as Selenotila intestinalis Krassilnikov was achieved. On the basis of mode of ascus formation and ascospore morphology it is included in the genus Metschnikowia Kamienski as a new species, M. lunata.
Topics: Spores, Fungal; Yeasts
PubMed: 603233
DOI: 10.1007/BF02313758 -
Foods (Basel, Switzerland) Oct 2023Three strains marketed as bioprotection yeasts were studied to compare their antimicrobial effect on a mixture of two yeast strains in synthetic must at 12 °C,...
Three strains marketed as bioprotection yeasts were studied to compare their antimicrobial effect on a mixture of two yeast strains in synthetic must at 12 °C, mimicking pre-fermentative maceration by combining different approaches. The growth of the different strains was monitored, their nitrogen and oxygen requirements were characterised, and their metabolomic footprint in single and co-cultures studied. Only the strain and one strains colonised the must and induced the rapid decline of . The efficiency of these two strains followed different inhibition kinetics. Furthermore, the initial ratio between and was an important factor to ensure optimal bioprotection. Nutrient consumption kinetics showed that apiculate yeasts competed with strains for nutrient accessibility. However, this competition did not explain the observed bioprotective effect, because of the considerable nitrogen content remaining on the single and co-cultures. The antagonistic effect of on probably implied another form of amensalism. For the first time, metabolomic analyses of the interaction in a bioprotection context were performed after the pre-fermentative maceration step. A specific footprint of the interaction was observed, showing the strong impact of the interaction on the metabolic modulation of the yeasts, especially on the nitrogen and vitamin pathways.
PubMed: 37959046
DOI: 10.3390/foods12213927 -
International Journal of Systematic and... Oct 2019Four strains, NYNU 15610, NYNU 15612, NYNU 15613 and NYNU 15615, of a novel ascomycetous yeast were isolated from the gut of (Coleoptera: Scarabeidae) collected from...
Four strains, NYNU 15610, NYNU 15612, NYNU 15613 and NYNU 15615, of a novel ascomycetous yeast were isolated from the gut of (Coleoptera: Scarabeidae) collected from two different localities in Henan Province, Central PR China. The four strains shared identical sequences in both of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions. Sequence analyses revealed that this novel species represents a member of the genus . It differed from its closest known species , and , by 8.4-9.2 % sequence divergence (33-40 nt substitutions and 7-12 gaps over 509 bases) in the D1/D2 sequences. The formation of ascospores was not observed on various sporulation media. In contrast to , and , the novel yeast species was unable to assimilate succinate, ethanol, ethylamine, cadaverine and 10 % NaCl plus 5 % glucose, but was able to grow in vitamin-free medium. The name f.a., sp. nov. is proposed to accommodate these strains, with NYNU 15613 as the holotype.
Topics: Animals; China; Coleoptera; DNA, Fungal; DNA, Ribosomal Spacer; Metschnikowia; Mycological Typing Techniques; Phylogeny; Sequence Analysis, DNA; Spores, Fungal
PubMed: 31329532
DOI: 10.1099/ijsem.0.003593 -
Scientific Reports May 2021Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental...
Biological control against microbial infections has a great potential as an alternative approach instead of fungicidal chemicals, which can cause environmental pollution. The pigment producer Metschnikowia andauensis belongs to the antagonistic yeasts, but details of the mechanism by which it inhibits growth of other microbes are less known. Our results confirmed its antagonistic capacity on other yeast species isolated from fruits or flowers and demonstrated that the antagonistic capacity was well correlated with the size of the red pigmented zone. We have isolated and characterized its red pigment, which proved to be the iron chelating pulcherrimin. Its production was possible even in the presence of 0.05 mg/ml copper sulphate, which is widely used in organic vineyards because of its antimicrobial properties. Production and localisation of the pulcherrimin strongly depended on composition of the media and other culture factors. Glucose, galactose, disaccharides and the presence of pectin or certain amino acids clearly promoted pigment production. Higher temperatures and iron concentration decreased the diameter of red pigmented zones. The effect of pH on pigment production varied depending of whether it was tested in liquid or solid media. In addition, our results suggest that other mechanisms besides the iron depletion of the culture media may contribute to the antagonistic capacity of M. andauensis.
Topics: Amino Acids, Sulfur; Carbon; Cell Count; Copper; Extracellular Space; Hydrogen-Ion Concentration; Ions; Iron; Metschnikowia; Piperidines; Polysaccharides; Temperature; Yeasts
PubMed: 34011985
DOI: 10.1038/s41598-021-89982-y