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Pathologie Et Biologie Apr 1968
Topics: Radiometry
PubMed: 4882388
DOI: No ID Found -
Medicamundi 1965
Topics: Radiometry
PubMed: 5857672
DOI: No ID Found -
Bulletin of Mathematical Biology 1973
Topics: Animals; Body Burden; Bone and Bones; Citrates; Dogs; Dose-Response Relationship, Radiation; Humans; Injections, Intravenous; Mathematics; Models, Biological; Radiation Dosage; Radiation Effects; Radium; Thorium; Time Factors
PubMed: 4783695
DOI: 10.1007/BF02558802 -
Current Protocols in Cell Biology May 2001The use of radioisotopes to label specific molecules in a defined way has greatly advanced the discovery and dissection of biochemical pathways. The development of... (Review)
Review
The use of radioisotopes to label specific molecules in a defined way has greatly advanced the discovery and dissection of biochemical pathways. The development of methods to inexpensively synthesize such tagged biological compounds on an industrial scale has enabled them to be used routinely in laboratory protocols, including many detailed in this manual. Although most of these protocols involve the use of only microcurie (mCi) amounts of radioactivity, some (particularly those describing the metabolic labeling of proteins or nucleic acids within cells) require amounts on the order of millicuries (mCi). In all cases where radioisotopes are used, depending on the quantity and nature of the isotope, certain precautions must be taken to ensure the safety of the investigator. It is essential to use good safety practices and proper protection to handle radioactive substances. This unit discusses storage, handling, and disposal of 35S, 32P, 33P, and 125I.
Topics: Animals; Biochemistry; Clinical Laboratory Techniques; Humans; Isotope Labeling; Molecular Biology; Radiation Monitoring; Radiation Protection; Radioactive Tracers; Radioactive Waste; Radioisotopes; Safety Management
PubMed: 18228276
DOI: 10.1002/0471143030.cba01ds00 -
Science (New York, N.Y.) May 1988Alpha particles are energetic short-range ions whose higher linear energy transfer produces extreme cytotoxicity. An alpha-particle-emitting radioimmunoconjugate...
Alpha particles are energetic short-range ions whose higher linear energy transfer produces extreme cytotoxicity. An alpha-particle-emitting radioimmunoconjugate consisting of a bismuth-212-labeled monoclonal immunoglobulin M specific for the murine T cell/neuroectodermal surface antigen Thy 1.2 was prepared. Analysis in vitro showed that the radioimmunoconjugate was selectively cytotoxic to a Thy 1.2+ EL-4 murine tumor cell line. Approximately three bismuth-212-labeled immunoconjugates per target cell reduced the uptake of [3H]thymidine by the EL-4 target cells to background levels. Mice inoculated intraperitoneally with EL-4 cells were cured of their ascites after intraperitoneal injection of 150 microcuries of the antigen-specific radioimmunoconjugate, suggesting a possible role for such conjugates in intracavitary cancer therapy.
Topics: Alpha Particles; Animals; Antigens, Surface; Bismuth; Immunoglobulin M; Immunotherapy; Lymphoma; Mice; Mice, Inbred C57BL; Radioisotopes; Thy-1 Antigens
PubMed: 2897133
DOI: 10.1126/science.2897133 -
Cancer Research Jan 1964
Topics: Adenoma; Carcinoma; Carcinoma, Papillary; Hyperplasia; Hypertrophy; Iodine Isotopes; Neoplasms; Neoplasms, Experimental; Pathology; Rats; Research; Thyroid Neoplasms; Thyroidectomy; Thyrotropin
PubMed: 14106158
DOI: No ID Found -
American Industrial Hygiene Association... Dec 1961
Topics: Carbon; Carbon Radioisotopes; Incineration; Radioactive Waste; Radioactivity
PubMed: 14462932
DOI: 10.1080/00028896109343442 -
Federal Register Dec 1997The Nuclear Regulatory Commission (NRC) is amending its regulations to permit NRC licensees to distribute a radioactive drug containing one microcurie of carbon-14 urea...
The Nuclear Regulatory Commission (NRC) is amending its regulations to permit NRC licensees to distribute a radioactive drug containing one microcurie of carbon-14 urea to any person for "in vivo" diagnostic use. The NRC has determined that the radioactive component of such a drug in capsule form presents an insignificant radiation risk and, therefore, regulatory control of the drug for radiation safety is not necessary. This amendment makes the drug more widely available and reduces costs to patients, insurers, and the health care industry. This action grants a petition for rulemaking (PRM-35-12) from Tri-Med Specialties, Inc. and completes action on the petition.
Topics: Carbon Radioisotopes; Diagnostic Techniques, Radioisotope; Drug Industry; Government Agencies; Humans; Licensure, Pharmacy; Radiopharmaceuticals; United States; Urea
PubMed: 10176576
DOI: No ID Found -
European Journal of Nuclear Medicine... Aug 2023To introduce a biomarker-based dosimetry method for the rational selection of a treatment activity for patients undergoing radioactive iodine I therapy (RAI) for...
The use of single-timepoint images to link administered radioiodine activity (MBq) to a prescribed lesion radiation-absorbed dose (cGy): a regression-based prediction interval tool for the management of well-differentiated thyroid cancer patients.
PURPOSE
To introduce a biomarker-based dosimetry method for the rational selection of a treatment activity for patients undergoing radioactive iodine I therapy (RAI) for metastatic differentiated thyroid cancer (mDTC) based on single-timepoint imaging of individual lesion uptake by I PET.
METHODS
Patients referred for RAI therapy of mDTC were enrolled in institutionally approved protocols. A total of 208 mDTC lesions (in 21 patients) with SUV > 1 underwent quantitative PET scans at 24, 48, 72, and 120 h post-administration of 222 MBq of theranostic NaI-I to determine the individual lesion radiation-absorbed dose. Using a general estimating equation, a prediction curve for biomarker development was generated in the form of a best-fit regression line and 95% prediction interval, correlating individual predicted lesion radiation dose metrics, with candidate biomarkers ("predictors") such as SUV and activity in microcurie per gram, from a single imaging timepoint.
RESULTS
In the 169 lesions (in 15 patients) that received I therapy, individual lesion cGy varied over 3 logs with a median of 22,000 cGy, confirming wide heterogeneity of lesion radiation dose. Initial findings from the prediction curve on all 208 lesions confirmed that a 48-h SUV was the best predictor of lesion radiation dose and permitted calculation of the I activity required to achieve a lesional threshold radiation dose (2000 cGy) within defined confidence intervals.
CONCLUSIONS
Based on MIRD lesion-absorbed dose estimates and regression statistics, we report on the feasibility of a new single-timepoint I-PET-based dosimetry biomarker for RAI in patients with mDTC. The approach provides clinicians with a tool to select personalized (precision) therapeutic administration of radioactivity (MBq) to achieve a desired target lesion-absorbed dose (cGy) for selected index lesions based on a single 48-h measurement I-PET image, provided the selected activity does not exceed the maximum tolerated activity (MTA) of < 2 Gy to blood, as is standard of care at Memorial Sloan Kettering Cancer Center.
TRIAL REGISTRATION
NCT04462471, Registered July 8, 2020. NCT03647358, Registered Aug 27, 2018.
Topics: Humans; Adenocarcinoma; Iodine Radioisotopes; Radiation Dosage; Thyroid Neoplasms
PubMed: 37171634
DOI: 10.1007/s00259-023-06240-1 -
Analytical Biochemistry Feb 1998UDP-GlcN[1-14C]Ac was synthesized in a single enzymatic reaction from [1-14C]acetate and commercially available precursors on both a microcurie (micromole) and a...
UDP-GlcN[1-14C]Ac was synthesized in a single enzymatic reaction from [1-14C]acetate and commercially available precursors on both a microcurie (micromole) and a millicurie (millimole) scale. The reaction was catalyzed by the action of acetyl coenzyme A synthetase, inorganic pyrophosphatase, and the bifunctional Escherichia coli GlmU protein. Within 2 h 86 to 94% reaction is attained, and it approaches 99% completion overnight. GlmU protein was prepared in the form of a fusion suitable for nickel chelate affinity chromatography. Several methods were developed for rapid purification of UDP-GlcN[1-14C]Ac: an HPLC method handled micromole (microcurie) loads. Alternatively, ion exchange chromatography over DOWEX AG1 X-2 using a batch elution procedure was compatible with millimole (millicurie) amounts of radiolabel and yielded both chemically and radiochemically homogeneous UDP-GlcN[1-14C]Ac. These methods allow laboratories to quickly produce and purify microcurie to millicurie quantities of N-acetyl-labeled UDP-GlcNAc by a choice of methods from relatively inexpensive precursors.
Topics: ATP-Binding Cassette Transporters; Amidohydrolases; Amino Acid Sequence; Base Sequence; Carbon Radioisotopes; Carrier Proteins; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cloning, Molecular; Escherichia coli; Escherichia coli Proteins; Maltose-Binding Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Nucleotidyltransferases; Recombinant Fusion Proteins; Uridine Diphosphate N-Acetylglucosamine
PubMed: 9473276
DOI: 10.1006/abio.1997.2525