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The Pharos of Alpha Omega Alpha-Honor... 2011
Topics: Bacteriology; Genes, Bacterial; Genetic Research; Genetics, Microbial; History, 20th Century; Humans; Male; Pneumococcal Infections; Streptococcus pneumoniae; Transformation, Bacterial
PubMed: 21615066
DOI: No ID Found -
Microbiologia (Madrid, Spain) Dec 1994
Topics: Animals; Ciliophora; France; Genetics, Microbial; History, 20th Century; Nobel Prize; Virology
PubMed: 7772298
DOI: No ID Found -
BioEssays : News and Reviews in... Oct 1988
Topics: Bacteria; Genetic Engineering; Genetics, Microbial; Microbiology; Molecular Biology
PubMed: 3228451
DOI: 10.1002/bies.950090407 -
Research in Microbiology Jun 2014
Topics: Gene Expression Regulation, Bacterial; Gene Expression Regulation, Viral; Genetics, Microbial; History, 20th Century; Molecular Biology; Operon; Prophages
PubMed: 24861002
DOI: 10.1016/j.resmic.2014.05.010 -
Current Biology : CB Jun 2003Richard Lenski is the John Hannah Distinguished Professor of Microbial Ecology at Michigan State University. He studies the ecology, genetics and evolution of bacteria...
Richard Lenski is the John Hannah Distinguished Professor of Microbial Ecology at Michigan State University. He studies the ecology, genetics and evolution of bacteria in an experimental setting that enables him to observe the dynamical processes and outcomes across many generations. One of his experiments with Escherichia coli has passed 30,000 generations and is still on-going. A few years ago, he also began studying artificial life in the form of 'digital organisms' - computer programs that replicate, mutate, compete, and therefore evolve and adapt.
Topics: Bacteria; Biological Evolution; Genetics, Microbial
PubMed: 12814560
DOI: 10.1016/s0960-9822(03)00401-9 -
Cold Spring Harbor Protocols May 2016Extraction of DNA from Schizosaccharomyces pombe cells is required for various uses, including templating polymerase chain reactions (PCRs), Southern blotting, library...
Extraction of DNA from Schizosaccharomyces pombe cells is required for various uses, including templating polymerase chain reactions (PCRs), Southern blotting, library construction, and high-throughput sequencing. To purify high-quality DNA, the cell wall is removed by digestion with Zymolyase or Lyticase and the resulting spheroplasts lysed using sodium dodecyl sulfate (SDS). Cell debris, SDS, and SDS-protein complexes are subsequently precipitated by the addition of potassium acetate and removed by centrifugation. Finally, DNA is precipitated using isopropanol. At this stage, purity is usually sufficient for PCR. However, for more sensitive procedures, such as restriction enzyme digestion, additional purification steps, including proteinase K digestion and phenol-chloroform extraction, are recommended. All of these steps are described in detail here.
Topics: DNA, Fungal; Genetics, Microbial; Molecular Biology; Schizosaccharomyces
PubMed: 27140918
DOI: 10.1101/pdb.prot090985 -
British Medical Bulletin Sep 1973
Topics: Genetics, Microbial; Genetics, Population; Molecular Biology; Research
PubMed: 4807320
DOI: 10.1093/oxfordjournals.bmb.a071004 -
Bacteriological Reviews Jun 1970
Review
Topics: Chromosome Mapping; Escherichia coli; Genetic Linkage; Genetics, Microbial; Terminology as Topic
PubMed: 4918631
DOI: 10.1128/br.34.2.155-175.1970 -
Computational and Mathematical Methods... 2021Genomic islands are related to microbial adaptation and carry different genomic characteristics from the host. Therefore, many methods have been proposed to detect... (Comparative Study)
Comparative Study
Genomic islands are related to microbial adaptation and carry different genomic characteristics from the host. Therefore, many methods have been proposed to detect genomic islands from the rest of the genome by evaluating its sequence composition. Many sequence features have been proposed, but many of them have not been applied to the identification of genomic islands. In this paper, we present a scheme to predict genomic islands using the chi-square test and random forest algorithm. We extract seven kinds of sequence features and select the important features with the chi-square test. All the selected features are then input into the random forest to predict the genome islands. Three experiments and comparison show that the proposed method achieves the best performance. This understanding can be useful to design more powerful method for the genomic island prediction.
Topics: Algorithms; Chi-Square Distribution; Computational Biology; Databases, Genetic; Genetics, Microbial; Genome, Bacterial; Genomic Islands; Genomics; Models, Genetic
PubMed: 34122622
DOI: 10.1155/2021/9969751 -
Current Protocols in Microbiology 2013Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a small (~7.5-kb) genome of positive polarity. cDNA clones of several strains are...
Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a small (~7.5-kb) genome of positive polarity. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro-transcribed viral genomes into an appropriate host cell. The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented.
Topics: Genetics, Microbial; Mutation; Poliovirus; Virology
PubMed: 23686829
DOI: 10.1002/9780471729259.mc15h02s29