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The Journal of Antimicrobial... Oct 2023
Topics: Humans; Enterobacteriaceae; Enterobacteriaceae Infections; China; Red Meat
PubMed: 37452728
DOI: 10.1093/jac/dkad222 -
Animals : An Open Access Journal From... Jan 2022is an Enterobacteriaceae with unclarified dispersion and pathogenicity. During an ongoing investigation about antimicrobial resistance in Greece, the occurrence of was...
is an Enterobacteriaceae with unclarified dispersion and pathogenicity. During an ongoing investigation about antimicrobial resistance in Greece, the occurrence of was evaluated among wild birds and humans. A total of 445 wild bird and 2000 human fecal samples were collected and screened for the presence of the organism. Subsequently, all strains were phenotypically and molecularly characterized regarding their antimicrobial resistance characteristics. Four were isolated from a common pheasant (), two Eurasian magpies () and a great white-fronted goose (). Among these four strains, the three latter presented resistance to 3rd generation cephalosporins, were phenotypically confirmed to produce ESBLs and were found to harbor . The three ESBL isolates additionally exhibited resistance to tetracyclines, while resistance to aminoglycosides was detected in two of them and to trimethoprim/sulfamethoxazole in one. No strains were retrieved from the human samples tested. This is the first report that provides evidence of dissemination among wild birds in Greece, describing CTX-M-1 production in multidrug resistant wild birds' isolates of this bacterial species.
PubMed: 35158664
DOI: 10.3390/ani12030340 -
Microorganisms Apr 2022, a member of the family of , although isolated widely in nature, rarely causes infections in humans. Herein, we report a case of isolation of from pigtail end culture,...
BACKGROUND
, a member of the family of , although isolated widely in nature, rarely causes infections in humans. Herein, we report a case of isolation of from pigtail end culture, urine culture and blood culture in a 76-year-old patient.
OBJECTIVE
To systematically address all the relevant information regarding through literature.
METHODS
We searched PubMed and Scopus databases up to January 2022 and performed a qualitative synthesis of published articles reporting infection from in humans.
RESULTS
We identified 25 records on PubMed and 43 additional records on Scopus. After removing duplicates, we examined in detail 15 articles. Ten studies with a total of 17 cases were included in our systematic review. Nine studies described isolated case reports, while 1 study described 8 cases. The origin of the infection was the alimentary tract in 9 cases, gallbladder in 4 cases, peritoneal cavity in 2 cases, respiratory tract in 1 case and hemodialysis catheter insertion site in 1 case. In 3 of the aforementioned cases was also isolated in blood cultures.
CONCLUSION
Physicians should be aware that can be present in multiple clinical specimens and that the antibiotic resistance profile of the isolates may pose significant challenges.
PubMed: 35630337
DOI: 10.3390/microorganisms10050892 -
Infection Dec 2009Moellerella wisconsensis, a member of the Enterobacteriaceae family, is rarely isolated in clinical specimens. We report here a case of M. wisconsensis infection in a... (Review)
Review
Moellerella wisconsensis, a member of the Enterobacteriaceae family, is rarely isolated in clinical specimens. We report here a case of M. wisconsensis infection in a 46-year-old cirrhotic patient with acute cholecystitis. This is the first reported case of a M. wisconsensis infection in Belgium and the first reported case of human bacteremia caused by this bacterium. Our case report is followed by a review of the literature.
Topics: Bacteremia; Belgium; Cholecystitis; Enterobacteriaceae; Enterobacteriaceae Infections; Humans; Liver Cirrhosis; Male; Middle Aged
PubMed: 19730786
DOI: 10.1007/s15010-009-8446-3 -
Vector Borne and Zoonotic Diseases... 2002This report describes the isolation of Moellerella wisconsensis from the oral secretions of a wild raccoon in Northern California. Human enteric disease has previously...
This report describes the isolation of Moellerella wisconsensis from the oral secretions of a wild raccoon in Northern California. Human enteric disease has previously been associated with this organism. This represents the first isolation of this rare enterobacterial species from a non-captive animal and only the third from a non-human source.
Topics: Animals; Animals, Wild; California; Female; Gram-Negative Bacteria; Gram-Negative Bacterial Infections; Mouth; Raccoons
PubMed: 12737549
DOI: 10.1089/15303660260613765 -
Diagnostic Microbiology and Infectious... Jan 2003The present study establishes a data compilation on biochemical features and natural antibiotic susceptibilities of Moellerella wisconsensis strains. 17 moellerellae... (Comparative Study)
Comparative Study
The present study establishes a data compilation on biochemical features and natural antibiotic susceptibilities of Moellerella wisconsensis strains. 17 moellerellae isolated from humans (n = 11), food (n = 5) and water (n = 1) were tested. Identification was carried out using two commercially available systems and conventional tests. MIC determinations of 74 antibiotics were performed applying a microdilution procedure in Cation-adjusted Mueller Hinton broth and IsoSensitest broth. M. wisconsensis was naturally sensitive to doxycycline, minocycline, all tested aminoglycosides, numerous beta-lactams, all fluoroquinolones, folate-pathway inhibitors, chloramphenicol and nitrofurantoin. Natural resistance was found with oxacillin, penicillin G, all tested macrolides, lincomycin, streptogramins, ketolides, glycopeptides, fusidic acid, linezolid and rifampicin. Medium-dependent differences in susceptibility affecting clinical assessment criteria were seen with tetracycline, clindamycin and fosfomycin. From the data of the present study it is possible that some moellerellae are misidentified as Klebsiella pneumoniae subsp. ozaenae.
Topics: Anti-Bacterial Agents; Bacteriological Techniques; Culture Media; Drug Resistance, Microbial; Enterobacteriaceae; Humans; Microbial Sensitivity Tests; Sensitivity and Specificity
PubMed: 12573545
DOI: 10.1016/s0732-8893(02)00483-2 -
Journal of Wildlife Diseases Oct 2020Total genomic (g)DNA from 100 American dog ticks (Dermacentor variabilis) collected from humans, dogs, raccoons, and skunks near Minnedosa (Manitoba, Canada) in 2005 was...
American Dog Ticks (Dermacentor variabilis) as Biological Indicators of an Association between the Enteric Bacterium Moellerella wisconsensis and Striped Skunks (Mephitis mephitis) in Southwestern Manitoba, Canada.
Total genomic (g)DNA from 100 American dog ticks (Dermacentor variabilis) collected from humans, dogs, raccoons, and skunks near Minnedosa (Manitoba, Canada) in 2005 was tested for the presence of Moellerella wisconsensis (Gammaproteobacteria: Enterobacteriales) using PCR. Although two gDNA samples derived from ticks attached to two striped skunks (Mephitis mephitis) contained M. wisconsensis DNA, it is unlikely that D. variabilis is a vector of this bacterium. Genomic DNA prepared from the washes of the external surfaces of these two ticks (i.e., before DNA extraction from the whole tick) and another two ticks attached to same skunks were also PCR positive for M. wisconsensis. This suggests that ticks acquired the bacterium by physical contact with contaminated or infected skunks. However, it does not exclude the possibility that the ticks may have also imbibed the bacterium from their host blood and lymph. Nonetheless, the results of this molecular study suggest that the four adult D. variabilis represent biological indicators of the presence of M. wisconsensis in association with their vertebrate hosts (i.e., striped skunks). Additional work is needed to determine if M. wisconsensis is present in the blood and lymph of striped skunks in southwestern Manitoba and if there are potential health risks for persons coming into contact with infected animals.
Topics: Animals; DNA, Bacterial; Dermacentor; Dogs; Gammaproteobacteria; Host-Pathogen Interactions; Humans; Manitoba; Mephitidae; Phylogeny; Raccoons
PubMed: 32402233
DOI: 10.7589/2019-09-224 -
Euro Surveillance : Bulletin Europeen... Dec 2022IntroductionMeat can be a vehicle for food-borne transmission of antimicrobial resistant bacteria and antimicrobial resistance genes. The occurrence of extended-spectrum...
Finding of extended-spectrum beta-lactamase (ESBL)-producing Enterobacterales in wild game meat originating from several European countries: predominance of producing CTX-M-1, November 2021.
IntroductionMeat can be a vehicle for food-borne transmission of antimicrobial resistant bacteria and antimicrobial resistance genes. The occurrence of extended-spectrum beta-lactamase (ESBL) producing Enterobacterales has been observed in meat from livestock production but has not been well studied in meat from wild game.AimWe aimed to investigate, particularly in central Europe, to what extent ESBL-producing Enterobacterales may be present in wild game meat.MethodsA total of 111 samples of different types of game meat supplied by butchers, hunters, retail stores and a large game-processing establishment in Europe were screened for ESBL-producing Enterobacterales using a selective culture medium. Isolates were genotypically and phenotypically characterised.ResultsThirty-nine samples (35% of the total) yielded ESBL-producing Enterobacterales, with most (35/39) supplied by the game-processing establishment. Isolates included 32 , 18 and one . PCR screening identified (n = 31), (n = 8), (n = 4), (n = 3), (n = 1), (n = 1), (n = 1), and (n = 2). Most belonged to phylogenetic group A (n = 7) or B1 (n = 9), but several isolates belonged to extraintestinal pathogenic (ExPEC) sequence types (ST)58 (n = 4), ST68 (n = 1) and ST540 (n = 1). Whole genome sequencing of six selected isolates localised on megaplasmids in four and on IncN_1 plasmids in one and one . Forty-eight isolates (94%) exhibited a multidrug-resistance phenotype.ConclusionWe found a high occurrence of ESBL-producing Enterobacterales in wild game meat, suggesting wildlife habitat pollution and possible microbial contamination events occurring during skinning or cutting carcasses.
Topics: Humans; Escherichia coli; Escherichia coli Infections; Phylogeny; beta-Lactamases; Enterobacteriaceae; Meat; Europe
PubMed: 36695441
DOI: 10.2807/1560-7917.ES.2022.27.49.2200343 -
Journal of Clinical Microbiology Apr 1984The name Moellerella wisconsensis is proposed for a group of the family Enterobacteriaceae previously called enteric group 46. The species name, wisconsensis, was coined...
The name Moellerella wisconsensis is proposed for a group of the family Enterobacteriaceae previously called enteric group 46. The species name, wisconsensis, was coined because six of the nine strains were isolated in Wisconsin. M. wisconsensis strains were negative for indole production, Voges-Proskauer, H2S production, urea, phenylalanine deaminase, lysine and ornithine decarboxylases, arginine dihydrolase, gas production from D-glucose, acid production from trehalose, and motility; the strains were positive for methyl red, citrate (Simmons), and acid production from lactose and raffinose and resistant to colistin. DNAs from five strains of M. wisconsensis were highly related (80 to 93% in reactions assayed on hydroxyapatite at 60 degrees C and 78 to 97% at 75 degrees C) to 32P-labeled DNA of the proposed type strain (CDC 2896-78, ATCC 35017). Labeled DNA from this type strain was only 2 to 32% related (at 60 degrees C) to DNA from 49 strains of named and unnamed species of Enterobacteriaceae. Eight of nine M. wisconsensis strains were isolated from human stool samples. Clinical information on one strain was available, and it was found to be associated with a case of diarrhea. On MacConkey agar, colonies of M. wisconsensis were bright red with precipitated bile around them and thus were indistinguishable from Escherichia coli colonies. Future studies should focus on the isolation of this new organism and its relationship to human disease.
Topics: Adult; Anti-Bacterial Agents; Child, Preschool; DNA, Bacterial; Diarrhea; Enterobacteriaceae; Feces; Female; Gastroenteritis; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Nucleic Acid Hybridization
PubMed: 6715516
DOI: 10.1128/jcm.19.4.460-463.1984 -
Journal of Infection in Developing... Feb 2020Infections caused by carbapenemase-producing Enterobacteriaceae have become a major threat to public health, worldwide. Here we report clinically significant NDM-1 and...
Infections caused by carbapenemase-producing Enterobacteriaceae have become a major threat to public health, worldwide. Here we report clinically significant NDM-1 and VIM-1 producing Moellerella wisconsensis which has not yet been described in the literature; this is the first report of M. wisconsensis strain harbouring blaNDM-1 and blaVIM-1, recovered from the rectal swab of a low birth weight female child admitted in NICU of the north Indian tertiary care hospital. A plasmid of IncW incompatibility with size of 154 kb was observed in AK-92 strain.
Topics: Bacterial Proteins; Drug Resistance, Multiple, Bacterial; Enterobacteriaceae Infections; Female; Gammaproteobacteria; Humans; India; Infant, Newborn; Intensive Care Units, Neonatal; Plasmids; beta-Lactamases
PubMed: 32146459
DOI: 10.3855/jidc.10969