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Internal Medicine (Tokyo, Japan) Dec 2002
Topics: Animals; Fusion Proteins, bcr-abl; Gene Expression Regulation, Neoplastic; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukocytosis; Mice; Monocytes
PubMed: 12521192
DOI: 10.2169/internalmedicine.41.1092 -
Leukemia Research Nov 2007We recently developed a modified Dupriez prognostic scoring system (PSS) that effectively discriminated between high-, intermediate-, and low-risk young patients (age <...
We recently developed a modified Dupriez prognostic scoring system (PSS) that effectively discriminated between high-, intermediate-, and low-risk young patients (age < or =60 years) with primary myelofibrosis (PMF) based on the respective presence of none, one, or two or more of the following parameters: hemoglobin <10 g/dL, leukocyte count <4 or >30 x 10(9)L(-1), and platelet count <100 x 10(9)L(-1). The current study (n=129; median age, 52 years; 69 males) reveals, on multivariable analysis, that an absolute monocyte count of > or =1 x 10(9)L(-1) carries an independent predictive value (p=0.02), for an inferior survival, in addition to that provided by hemoglobin level (p=0.002), platelet count (0.02), and leukocyte count (p=0.16). The inclusion of the monocyte count as a fourth risk factor enabled the construction of a new and improved Mayo PSS; median survival was 173, 61, and 26 months in the absence of all four (low-risk), three (intermediate-risk), or two or less (high-risk) adverse features, respectively (p<0.0001). The independent prognostic value of monocytosis was validated in a separate database of 97 patients with PMF from another institution.
Topics: Adolescent; Adult; Female; Humans; Male; Middle Aged; Monocytes; Primary Myelofibrosis; Prognosis
PubMed: 17397921
DOI: 10.1016/j.leukres.2006.12.025 -
British Journal of Industrial Medicine Jan 1946
Topics: Explosive Agents; Humans; Leukemia; Toluene; Trinitrotoluene
PubMed: 20983526
DOI: 10.1136/oem.3.1.24 -
Allergy Jun 2024Respiratory syncytial virus (RSV) infection in infants is a major cause of viral bronchiolitis and hospitalisation. We have previously shown in a murine model that...
BACKGROUND
Respiratory syncytial virus (RSV) infection in infants is a major cause of viral bronchiolitis and hospitalisation. We have previously shown in a murine model that ongoing infection with the gut helminth Heligmosomoides polygyrus protects against RSV infection through type I interferon (IFN-I) dependent reduction of viral load. Yet, the cellular basis for this protection has remained elusive. Given that recruitment of mononuclear phagocytes to the lung is critical for early RSV infection control, we assessed their role in this coinfection model.
METHODS
Mice were infected by oral gavage with H. polygyrus. Myeloid immune cell populations were assessed by flow cytometry in lung, blood and bone marrow throughout infection and after secondary infection with RSV. Monocyte numbers were depleted by anti-CCR2 antibody or increased by intravenous transfer of enriched monocytes.
RESULTS
H. polygyrus infection induces bone marrow monopoiesis, increasing circulatory monocytes and lung mononuclear phagocytes in a IFN-I signalling dependent manner. This expansion causes enhanced lung mononuclear phagocyte counts early in RSV infection that may contribute to the reduction of RSV load. Depletion or supplementation of circulatory monocytes prior to RSV infection confirms that these are both necessary and sufficient for helminth induced antiviral protection.
CONCLUSIONS
H. polygyrus infection induces systemic monocytosis contributing to elevated mononuclear phagocyte numbers in the lung. These cells are central to an anti-viral effect that reduces the peak viral load in RSV infection. Treatments to promote or modulate these cells may provide novel paths to control RSV infection in high risk individuals.
PubMed: 38924546
DOI: 10.1111/all.16206 -
Blood Coagulation & Fibrinolysis : An... Sep 2022We aimed to investigate the relationship between demographics, clinical features, laboratory findings including monocytosis and clinical course in children with immune...
We aimed to investigate the relationship between demographics, clinical features, laboratory findings including monocytosis and clinical course in children with immune thrombocytopenia (ITP). Data of 100 ITP patients were analysed. Complete blood count findings of the patients at certain time points were evaluated to classify the disease as acute, persistent and chronic. An effect of sex on chronicity was not observed ( P = 0.166). Of the patients enrolled in the study, 38% ( n = 38) had chronic course. The mean age of patients with the chronic course was 7 ± 4.1 years, which was significantly higher than the other groups ( P = 0.007). Sixty-five percent ( n = 13) of the patients presenting with mucosal bleeding and 27.4% ( n = 20) of the patients presenting with skin bleeding became chronic ( P = 0.008). MPV was found to be significantly high in chronic ITP patients ( P = 0.049). Monocytosis was noted in 80% of the patients at diagnosis. Intravenous immunoglobulin was used in 84% of the patients with acute ITP; 33% of them developed chronic ITP. The age at diagnosis, presence of mucosal bleeding and increased MPV on admission were high-risk factors for the development of the chronic course. Monocytosis was detected in 80% of the patients on admission, and it may play a role in the pathogenesis of ITP.
Topics: Child; Child, Preschool; Hemorrhage; Humans; Immunoglobulins, Intravenous; Platelet Count; Purpura, Thrombocytopenic, Idiopathic; Risk Factors
PubMed: 35834725
DOI: 10.1097/MBC.0000000000001146 -
Blood Oct 1995
Topics: B-Lymphocytes; Humans; Leukocyte Count; Macrophage Colony-Stimulating Factor; Male; Middle Aged; Monocytes; Staining and Labeling; Waldenstrom Macroglobulinemia
PubMed: 7545477
DOI: No ID Found -
International Journal of Laboratory... Dec 2020Monocytosis Workflow Optimization rule set has been developed by using mono-dysplasia-score to determine reactive monocytosis and prevent unnecessary blood smear of... (Clinical Trial)
Clinical Trial
A new approach for diagnosing hematological malignancies using monocytosis workflow optimization and abnormal lymphocyte/blast flag of Sysmex XN series of blood count analyzers.
INTRODUCTION
Monocytosis Workflow Optimization rule set has been developed by using mono-dysplasia-score to determine reactive monocytosis and prevent unnecessary blood smear of these patients and for detection of chronic myelomonocytic leukemia cases during complete blood count. In our study, we aimed to examine the contribution of Monocytosis Workflow Optimization rule set.
METHODS
Adult patients with monocyte count ≥1.0 10 /µL and monocyte percentage ≥10% were included in our study. Blood smears were made from the samples in our laboratory. These smears were examined and patients were divided into two groups as reactive monocytosis or hematological malignancy. The groups were compared in terms of Monocytosis Workflow Optimization rule set and device flags.
RESULTS
Twenty-one patients had hematological malignancies of 155 patients who were included in our study. Monocytosis Workflow Optimization rule set suggested performing blood smear in 19 of the patients with hematological malignancy, and evaluated two patients as reactive monocytosis with 90.5% sensitivity and 76.9% specificity. There was an "abnormal lymphocyte/ blast" flag in 90.5% of patients with hematological malignancies and in patients whose Monocytosis Workflow Optimization rule set defined as reactive monocytosis and it was found that sensitivity and negative predictive value reached 100%.
CONCLUSION
Automated validation support systems and softwares developed especially for these systems make it possible to classify patients with their non-specific findings, as a result both contributing to the reduction of laboratory workload and costs and assisting laboratory specialists and clinicians with adding value to laboratory results.
Topics: Aged; Automation, Laboratory; Female; Hematologic Neoplasms; Humans; Leukocyte Count; Lymphocytes; Male; Middle Aged; Prospective Studies
PubMed: 32639667
DOI: 10.1111/ijlh.13281 -
Avian Pathology : Journal of the W.V.P.A Jan 1980Differential and total white cell counts on the peripheral blood of chickens, turkeys and quails maintained from hatching on an experimental zinc-deficient diet,...
Differential and total white cell counts on the peripheral blood of chickens, turkeys and quails maintained from hatching on an experimental zinc-deficient diet, revealed a severe monocytosis. In chickens the increase in monocytes was first detected on the 3rd day after hatching and was maintained until 18 days of age. The relationship of this monocytosis to the zinc-associated factors which affect mammalian leucocytes and to the damaged epithelium in zinc-deficient birds is discussed.
PubMed: 18770240
DOI: 10.1080/03079458008418386 -
Clinical Cancer Research : An Official... May 2018Preoperative or neoadjuvant therapy (NT) is increasingly used in patients with locally advanced or inflammatory breast cancer to allow optimal surgery and aim for...
Preoperative or neoadjuvant therapy (NT) is increasingly used in patients with locally advanced or inflammatory breast cancer to allow optimal surgery and aim for pathologic response. However, many breast cancers are resistant or relapse after treatment. Here, we investigated conjunctive chemotherapy-triggered events occurring systemically and locally, potentially promoting a cancer stem-like cell (CSC) phenotype and contributing to tumor relapse. We started by comparing the effect of paired pre- and post-NT patient sera on the CSC properties of breast cancer cells. Using cell lines, patient-derived xenograft models, and primary tumors, we investigated the regulation of CSCs and tumor progression by chemotherapy-induced factors. In human patients and mice, we detected a therapy-induced CSC-stimulatory activity in serum, which was attributed to therapy-associated monocytosis leading to systemic elevation of monocyte chemoattractant proteins (MCP). The post-NT hematopoietic regeneration in the bone marrow highlighted both altered monocyte-macrophage differentiation and biased commitment of stimulated hematopoietic stem cells toward monocytosis. Chemotherapeutic agents also induce monocyte expression of MCPs through a JNK-dependent mechanism. Genetic and pharmacologic inhibitions of the MCP-CCR2 pathway blocked chemotherapy's adverse effect on CSCs. Levels of nuclear Notch and ALDH1 were significantly elevated in primary breast cancers following NT, whereas higher levels of CCR2 in pre-NT tumors were associated with a poor response to NT. Our data establish a mechanism of chemotherapy-induced cancer stemness by linking the cellular events in the bone marrow and tumors, and suggest pharmacologic inhibition of CCR2 as a potential cotreatment during conventional chemotherapy in neoadjuvant and adjuvant settings. .
Topics: Animals; Antineoplastic Combined Chemotherapy Protocols; Biomarkers; Breast Neoplasms; Cell Line, Tumor; Cytokines; Disease Models, Animal; Female; Humans; Immunophenotyping; Leukocytosis; Mice; Monocytes; Neoplastic Stem Cells; Receptors, CCR2; Receptors, Notch; Signal Transduction; Xenograft Model Antitumor Assays
PubMed: 29500278
DOI: 10.1158/1078-0432.CCR-17-2545 -
Arthritis and Rheumatism Mar 1979Five patients with the triad of fever, skin rash, and acute polyarthritis were studied with regard to synovial fluid analysis. All cases revealed inflammatory effusions...
Five patients with the triad of fever, skin rash, and acute polyarthritis were studied with regard to synovial fluid analysis. All cases revealed inflammatory effusions with a predominant monocytosis. Skin biopsies from two cases and synovial membrane biopsy from one case revealed a nonnecrotizing vasculitis. Although the etiology of this syndrome was not ascertained, it bears striking similarities to certain viral arthritides. It also deserves consideration as a form of acute hypersensitivity angiitis.
Topics: Adult; Arthritis; Dermatitis; Female; Fever; Humans; Male; Middle Aged; Monocytes; Seasons; Synovial Fluid; Synovial Membrane; Vasculitis
PubMed: 420721
DOI: 10.1002/art.1780220314