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Journal of the Science of Food and... May 2020The composition of drip brew coffee versus brewing time has been chemically characterized in previous studies, and it is known that the total dissolved solids (TDS)...
BACKGROUND
The composition of drip brew coffee versus brewing time has been chemically characterized in previous studies, and it is known that the total dissolved solids (TDS) systematically decreases with each fraction during the brew. Little information exists regarding the corresponding sensory attributes versus time, however, and it is unclear how TDS correlates with flavor profile.
RESULTS
Standard drip brews were fractionated into distinct samples by switching in an empty carafe every 30 s during the brew. Using a trained sensory descriptive panel, we found that most taste and flavor attributes decreased with brew time; for example, the earlier fractions were systematically more bitter and more sour than later fractions. Surprisingly, however, several flavor and taste attributes increased in time; for example, later fractions were systematically sweeter and more floral than earlier fractions. Since later fractions had lower TDS, these results indicate that perceived sweetness in drip brew coffee is negatively correlated with TDS. Mass spectrometry measurements of the monosaccharide content in the brews showed that none of the fractions had perceptible concentrations of any monosaccharide.
CONCLUSION
The results of the sensory analysis and the monosaccharide analysis suggest that perceptible sweetness in coffee is a consequence of masking effects and/or the presence of sweet-associated aromas and flavors. The results further suggest that unique flavor profiles could be obtained from the same coffee grounds by judicious combinations of specific fractions. © 2020 Society of Chemical Industry.
Topics: Adult; Coffee; Cooking; Humans; Monosaccharides; Odorants; Taste; Time Factors
PubMed: 32031262
DOI: 10.1002/jsfa.10323 -
Carbohydrate Research Jan 2022Unlike DNA and proteins, there is a limit to inferring the structure and function of glycans only by analyzing their sequence. Due to their structural flexibility, it...
Unlike DNA and proteins, there is a limit to inferring the structure and function of glycans only by analyzing their sequence. Due to their structural flexibility, it can be said that an understanding of the 3D structural conformations of glycans is important to better understand their functions. While there are several tools now available that aid in analyzing the 3D structures of glycans, they are very computationally intensive and not easily useable by non-experts. Thus, as a first step, we decided to investigate the monosaccharides that make up the building blocks of glycans and their similarities. We developed a method and software that takes the three-dimensional structures of monosaccharides and finds their commonalities through an efficient algorithm, which we call TouCom (tou = "sugar" in Japanese). We then created a similarity matrix to represent the degree of similarity of pairs of monosaccharides based on this information and the properties of their functional groups. We performed an analysis of pairwise glycan alignment using this similarity matrix, confirming that the scores of pairwise-alignments obtained were improved compared to alignments without using this matrix. As a result, we propose the first monosaccharide substitution matrix that has been developed based on 3D atomic structure. In the future, we will apply this matrix to other glycan alignment tools so that glycan sequence analysis can better utilize this information. We expect that this monosaccharide substitution matrix can improve the analysis of glycan function based on glycan structural information.
Topics: Algorithms; Monosaccharides; Polysaccharides; Proteins; Software
PubMed: 35030433
DOI: 10.1016/j.carres.2021.108496 -
Journal of Separation Science Apr 2021Codium fragile is a green alga belonging to Codiales family. The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant,...
Codium fragile is a green alga belonging to Codiales family. The sulfated polysaccharides of this alga have anti-coagulation, antiviral, anti-angiogenesis, antioxidant, and immunoregulatory properties. In this study, we developed a reliable and rapid method for the analysis of 10 monosaccharides using ultra-performance liquid chromatography-tandem mass spectrometry in the negative electrospray ionization and multiple reaction monitoring mode. Monosaccharides, including two pentoses (xylose, arabinose); two deoxyhexoses (rhamnose, fucose); three hexoses (mannose, glucose, galactose); two hexuronic acids (glucuronic acid, galacturonic acid), and an N-acetyl-hexosamine (glucosamine), were derivatized using 1-phenyl-3-methyl-5-pyrazolone and simultaneously analyzed within 9 min. Optimization of the derivatization process, especially by using various 1-phenyl-3-methyl-5-pyrazolone concentrations, was studied. The calibration curves showed good linearity with a squared correlation coefficient > 0.995. The spiked recovery was determined to be 91.1-105.7% with the relative intra-day and inter-day standard deviations ranging from 2.58-6.71% and 3.15-7.67%, respectively. The limit of detection and limit of quantification for all 10 monosaccharides ranged from 0.02 to 0.10 μg/mL and 0.05 to 0.25 μg/mL, respectively. Using this method, the monosaccharides comprising the polysaccharides of Codium fragile were determined to be arabinose, galactose, and glucose.
Topics: Chlorophyta; Chromatography, High Pressure Liquid; Monosaccharides; Polysaccharides; Solubility; Tandem Mass Spectrometry; Water
PubMed: 33533562
DOI: 10.1002/jssc.202001140 -
Phytochemistry Sep 2013The succulent leaf mesophyll in Aloe species supports a burgeoning natural products industry, particularly in Africa. Comparative data necessary to prioritise species...
INTRODUCTION
The succulent leaf mesophyll in Aloe species supports a burgeoning natural products industry, particularly in Africa. Comparative data necessary to prioritise species with economic potential have been lacking.
OBJECTIVE
To survey leaf mesophyll monosaccharide composition in the genus Aloe using a predictive phylogenetic approach.
METHODOLOGY
Monosaccharide composition was assessed in 31 species, representing the morphological and taxonomic diversity of Aloe sensu stricto. Leaf mesophyll polysaccharides were partially hydrolysed in a trifluoroacetic acid (TFA)-SilA assay. Oximes and trimethylsilyl ether products were detected by GC-MS. Constituent monosaccharides accounting for the greatest variation among species were identified by principal component analysis. Two plant DNA barcoding regions were sequenced in 28 of the sampled species and the resulting maximum likelihood tree was used to evaluate phylogenetic signal in monosaccharide composition throughout the genus.
RESULTS
Nineteen peaks (Rt=16.76-23.67 min) were identified in the GC-MS spectra. All samples were dominated by one constituent; glucose was the major monosaccharide in 19 species, mannose in eight species, and xylose in one species (Aloidendron pillansii). Three monosaccharides therefore account for 90% of the variation in leaf mesophyll in Aloe. Species which do not share this typical monosaccharide profile appear to group outside the core Aloe clade in the phylogeny.
CONCLUSION
Preliminary findings suggest that leaf mesophyll monosaccharide composition is conservative in Aloe. Characterisation of within-species variation and quantitative differences between species will be necessary to authenticate leaf mesophyll products, whereas unusual monosaccharide profiles could be diagnostic in some species. The common glucose-mannose-xylose profile identified in commercially important species is shared by many other Aloe species.
Topics: Aloe; Gas Chromatography-Mass Spectrometry; Monosaccharides; Multivariate Analysis; Phylogeny; Plant Leaves
PubMed: 23642457
DOI: 10.1016/j.phytochem.2013.03.015 -
Methods in Molecular Biology (Clifton,... 2024Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous...
Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensitivity (limit of quantitation 1 nmol). This method allows for complex monosaccharide mixtures to be separated and quantified using a reasonably simple and safe derivatization procedure.
Topics: Chromatography, Reverse-Phase; Monosaccharides; Chromatography, High Pressure Liquid; Spectrophotometry, Ultraviolet; Edaravone; Antipyrine
PubMed: 38656509
DOI: 10.1007/978-1-0716-3782-1_5 -
Carbohydrate Research Dec 2015Xylose is one of the few monosaccharidic building blocks that are used by mammalian cells. In comparison with other monosaccharides, xylose is rather unusual and, so... (Review)
Review
Xylose is one of the few monosaccharidic building blocks that are used by mammalian cells. In comparison with other monosaccharides, xylose is rather unusual and, so far, only found in two different mammalian structures, i.e. in the Notch receptor and as the linker between protein and glycosaminoglycan (GAG) chains in proteoglycans. Interestingly, simple soluble xylopyranosides can not only initiate the biosynthesis of soluble GAG chains but also function as inhibitors of important enzymes in the biosynthesis of proteoglycans. Furthermore, xylose is a major constituent of hemicellulosic xylans and thus one of the most abundant carbohydrates on Earth. Altogether, this has spurred a strong interest in xylose chemistry. The scope of this review is to describe synthesis of xylopyranosyl donors, as well as protective group chemistry, modifications, and conformational analysis of xylose.
Topics: Animals; Glycosides; Humans; Molecular Structure; Pyrans; Xylose
PubMed: 26580709
DOI: 10.1016/j.carres.2015.10.004 -
Scientific Reports Jan 2023This research examined the possible pathway of monosaccharide production from the rice straw waste using three integrated enzymatic hydrolysis approaches: boiled hot...
This research examined the possible pathway of monosaccharide production from the rice straw waste using three integrated enzymatic hydrolysis approaches: boiled hot water pre-treatment with enzyme, alkaline pre-treatment with enzyme, and acid pre-treatment with enzyme, that can be further used as the feedstock for anaerobic digestion. Two cellulase enzymes: SIGMA-ALDRICH laboratory grade cellulase from Aspergillus niger and atres Zymix plus as a commercial cellulase enzyme were applied. It was found that the boiled hot water pre-treatment with the commercial cellulase gave the highest total monosaccharides yields. Glucose was the most significant part (78-86%) of the monosaccharides. For the pre-treatment with dilute acid, glucose was also the main component of monosaccharides; however, for the alkali pre-treatment, xylose was the main monosaccharide. It made up 48-85% of the total monosaccharide compared to glucose that made up 5-49% of total monosaccharide. Boiled rice straw with commercial cellulase enzyme provided the highest glucose yield compared to other experiments. Moreover, the obtained results from GC-MS/MS analysis show that up to 62 species of phenolic compound could be found in enzymatic hydrolysis of the rice straw waste. Aromatic and aliphatic hydrocarbon substances were also detected in the FEEM analysis. From the overall results, the integrated enzymatic hydrolysis with boil hot water pre-treatment was the most efficient method for monosaccharide production from the rice straw waste.
Topics: Monosaccharides; Oryza; Anaerobiosis; Hydrolysis; Tandem Mass Spectrometry; Cellulase; Glucose
PubMed: 36600032
DOI: 10.1038/s41598-023-27398-6 -
Carbohydrate Polymers Jan 2016A novel analytical method for neutral monosaccharide composition analysis of plant-derived oligo- and polysaccharides was developed using hydrophilic interaction liquid...
A novel analytical method for neutral monosaccharide composition analysis of plant-derived oligo- and polysaccharides was developed using hydrophilic interaction liquid chromatography coupled to a charged aerosol detector. The effects of column type, additives, pH and column temperature on retention and separation were evaluated. Additionally, the method could distinguish potential impurities in samples, including chloride, sulfate and sodium, from sugars. The results of validation demonstrated that this method had good linearity (R(2) ≥ 0.9981), high precision (relative standard deviation ≤ 4.43%), and adequate accuracy (94.02-103.37% recovery) and sensitivity (detection limit: 15-40 ng). Finally, the monosaccharide compositions of the polysaccharide from Eclipta prostrasta L. and stachyose were successfully profiled through this method. This report represents the first time that all of these common monosaccharides could be well-separated and determined simultaneously by high performance liquid chromatography without additional derivatization. This newly developed method is convenient, efficient and reliable for monosaccharide analysis.
Topics: Chlorides; Chromatography, High Pressure Liquid; Eclipta; Hydrogen-Ion Concentration; Monosaccharides; Oligosaccharides; Polysaccharides; Sodium; Sulfates; Temperature; Time Factors
PubMed: 26572471
DOI: 10.1016/j.carbpol.2015.10.028 -
Carbohydrate Polymers Apr 2014Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and...
Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and cell-cell interaction, with the carbohydrate structure determining main function in the cell. Due to the importance of the primary structure, the monosaccharide composition is crucial to show the glycan structure. We now present a method for complex carbohydrates based on NMR spectroscopy, which has shown to give similar results to those obtained by the classic GC-MS-carboxy-reduction/deuterium labeling approach. Quantitative HSQC, through JCH dependence showed 155 Hz as the best value for (1)H/(13)C anomeric aldoses, allowing milli-microM detection using conventional inverse probe heads. Combining the quantification of native monosaccharide units of the glycan and those from the hydrolyzed product, a strong correlation occurs between the molecular mobility of the monosaccharide units, giving rise to some insights on the dynamic properties of the parent glycan.
Topics: Magnetic Resonance Spectroscopy; Monosaccharides; Polysaccharides
PubMed: 24607157
DOI: 10.1016/j.carbpol.2013.12.046 -
Carbohydrate Polymers Jul 2020Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides. To modernize the hydrolytic...
Monosaccharide composition analysis after acid hydrolysis is the first step towards structural characterization of the polysaccharides. To modernize the hydrolytic procedure, we used a polymerase chain reaction (PCR) instrument to accomplish the task, which allows to generate monosaccharide products from up to 96 samples simultaneously within 30 min. Fucoidan, chitosan and propylene glycol alginate sodium sulfate (PSS) were chosen as representatives of complex, basic and acidic polysaccharides to optimize the hydrolytic conditions, respectively, through the orthogonal L9 (3) experiments. The hydrolysis loss ratio for monosaccharide standards were also measured. Using this assay, the hydrolysis plus 1-phenyl-3-methyl-5-pyrazolone (PMP) labeling of the monosaccharide products could be accomplished in 90 min with the RSD values less than 5 % based on HPLC analysis. We further confirmed the reliability of the assay by HPLC coupled MS analysis. In conclusion, PCR instrument-based hydrolysis assay is suitable for monosaccharide composition analysis of complex, acidic and basic polysaccharides.
Topics: Hydrolysis; Monosaccharides; Polymerase Chain Reaction; Polysaccharides
PubMed: 32475593
DOI: 10.1016/j.carbpol.2020.116338