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BMC Microbiology Apr 2022Mycobacterium fortuitum (M. fortuitum) is a bacterium, which can cause infections in many anatomical regions of the body, including the skin, lymph nodes, and...
BACKGROUND
Mycobacterium fortuitum (M. fortuitum) is a bacterium, which can cause infections in many anatomical regions of the body, including the skin, lymph nodes, and joints. This bacterium, which belongs to a group of bacteria known as nontuberculous mycobacteria, is regarded as an important nosocomial pathogen worldwide owing to its increasing antibiotic resistance. Recently, the antimicrobial effects of carbon nanotubes have been reported in numerous studies. These nanotubes can be very useful in drug delivery; besides, they exhibit unique properties against multidrug-resistant bacterial infections. This study aimed to investigate the antimicrobial effects of carboxyl-functionalized multi-walled carbon nanotubes (MWCNT-COOH) to reduce antibiotic resistance.
METHODS
In this study, antibacterial effects of nanofluids containing functionalized MWCNTs at initial concentration of 2 mg/mL and serial dilutions of 54, 28.5, 14.25, 7.12, 3.5 µg/mL, antibiotics alone and combination of nanofluids with antibiotics were investigated. Standard and resistant strains of M. fortuitum were obtained from the microbial bank of the Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran.
RESULTS
It was observed that nanofluid containing MWCNT-COOH can exert antimicrobial effects on M. fortuitum and significantly reduce bacterial resistance to antibiotics including kanamycin and streptomycin. In the presence of antibiotics and nanofluids containing MWCNT-COOH at a dose of 28.5 µg/mL, no growth was observed.
CONCLUSION
One of the main antimicrobial mechanisms of MWCNT-COOH is penetration into the bacterial cell wall. In this study, by using the nanofluid containing MWCNT-COOH with increased stability, the antibiotic resistance of M. fortuitum was significantly reduced at lower dilutions compared to the antibiotic alone.
Topics: Anti-Bacterial Agents; Anti-Infective Agents; Bacteria; Drug Resistance, Microbial; Mycobacterium fortuitum; Nanotubes, Carbon
PubMed: 35410123
DOI: 10.1186/s12866-022-02523-z -
Plastic and Reconstructive Surgery May 2002
Review
Topics: Adult; Breast Implantation; Drainage; Female; Humans; Mycobacterium Infections, Nontuberculous; Mycobacterium fortuitum; Reoperation; Sodium Chloride; Surgical Wound Infection
PubMed: 11994623
DOI: 10.1097/00006534-200205000-00051 -
BMC Nephrology Jun 2012Peritoneal dialysis-associated peritonitis (PD-peritonitis) due to Mycobacterium spp is uncommon. Non-tuberculous Mycobacterium (NTB) PD-peritonitis can present in a... (Review)
Review
BACKGROUND
Peritoneal dialysis-associated peritonitis (PD-peritonitis) due to Mycobacterium spp is uncommon. Non-tuberculous Mycobacterium (NTB) PD-peritonitis can present in a similar fashion to more common causes of bacterial PD-peritonitis. We describe the first reported case of multiresistant Mycobacterium fortuitum PD-peritonitis in an Australian patient.
CASE PRESENTATION
A 38 year-old woman developed mild PD-peritonitis during an overseas holiday. Treatment was complicated by delayed diagnosis, requirement for special investigations, treatment with multiple antibiotics, and conversion to haemodialysis following removal of her Tenckhoff catheter.
CONCLUSION
This case demonstrates the diagnostic yield of pursuing further investigations in cases of initially culture-negative, problematic PD-peritonitis. A systematic review of the literature identified only 17 reports of M. fortuitum PD-peritonitis. Similar to our case, a delay in microbiological diagnosis was frequently noted and the Tenckhoff catheter was commonly removed at the time of diagnosis. The type and duration of antibiotic therapy also varied widely so the optimum treatment appears to be poorly defined.
Topics: Adult; Female; Humans; Mycobacterium Infections, Nontuberculous; Mycobacterium fortuitum; Peritoneal Dialysis; Peritonitis
PubMed: 22682357
DOI: 10.1186/1471-2369-13-35 -
Clinical Microbiology and Infection :... Feb 2002Endocarditis due to Mycobacterium fortuitum complex is a rare entity generally linked to the hospital environment. Only 18 cases have been published since 1966. Here we... (Review)
Review
Endocarditis due to Mycobacterium fortuitum complex is a rare entity generally linked to the hospital environment. Only 18 cases have been published since 1966. Here we present a case of a female who developed an endocarditis due to Mycobacterium chelonae after valve replacement as well as a review of the literature. The course of this kind of endocarditis is generally subacute and the outcome is usually fatal. Blood cultures were positive in 75% of cases of metallic valve endocarditis, versus 20% in bioprostheses. The treatment must include antibiotics that have shown activity against these mycobacteria, such as amikacin, imipenem, cefoxitin, fluorinated quinolones and macrolides (especially clarithromycin). Surgical removal is recommended. Although the prognosis for the patient is poor, we should expect better outcomes with the use of new antibiotic regimens.
Topics: Aortic Valve Insufficiency; Endocarditis, Bacterial; Female; Heart Valve Prosthesis; Humans; Middle Aged; Mitral Valve Insufficiency; Mycobacterium Infections, Nontuberculous; Mycobacterium fortuitum; Prognosis
PubMed: 11952729
DOI: 10.1046/j.1198-743x.2001.00397.x -
Nefrologia : Publicacion Oficial de La... 2015
Review
Topics: Anti-Bacterial Agents; Catheter-Related Infections; Child, Preschool; Device Removal; Drug Resistance, Multiple, Bacterial; Drug Substitution; Drug Therapy, Combination; Granuloma; Humans; Male; Mycobacterium Infections, Nontuberculous; Mycobacterium fortuitum; Peritoneal Dialysis, Continuous Ambulatory
PubMed: 26565935
DOI: 10.1016/j.nefro.2015.09.010 -
Pediatric Allergy and Immunology :... Sep 2023
Topics: Female; Humans; Mycobacterium fortuitum; Histiocytosis; Genetic Predisposition to Disease
PubMed: 37747748
DOI: 10.1111/pai.14027 -
Applied Microbiology and Biotechnology Oct 2023Biofilm formation by Mycobacterium fortuitum causes serious threats to human health due to its increased contribution to nosocomial infections. In this study, the first... (Comparative Study)
Comparative Study
Biofilm formation by Mycobacterium fortuitum causes serious threats to human health due to its increased contribution to nosocomial infections. In this study, the first comprehensive global proteome analysis of M. fortuitum was reported under planktonic and biofilm growth states. A label-free Q Exactive Quadrupole-Orbitrap tandem mass spectrometry analysis was performed on the protein lysates. The differentially abundant proteins were functionally characterized and re-annotated using Blast2GO and CELLO2GO. Comparative analysis of the proteins among two growth states provided insights into the phenotypic switch, and fundamental pathways associated with pathobiology of M. fortuitum biofilm, such as lipid biosynthesis and quorum-sensing. Interaction network generated by the STRING database revealed associations between proteins that endure M. fortuitum during biofilm growth state. Hypothetical proteins were also studied to determine their functional alliance with the biofilm phenotype. CARD, VFDB, and PATRIC analysis further showed that the proteins upregulated in M. fortuitum biofilm exhibited antibiotic resistance, pathogenesis, and virulence. Heatmap and correlation analysis provided the biomarkers associated with the planktonic and biofilm growth of M. fortuitum. Proteome data was validated by qPCR analysis. Overall, the study provides insights into previously unexplored biochemical pathways that can be targeted by novel inhibitors, either for shortened treatment duration or for eliminating biofilm of M. fortuitum and related nontuberculous mycobacterial pathogens. KEY POINTS: • Proteomic analyses of M. fortuitum reveals novel biofilm markers. • Acetyl-CoA acetyltransferase acts as the phenotype transition switch. • The study offers drug targets to combat M. fortuitum biofilm infections.
Topics: Mycobacterium fortuitum; Biofilms; Microscopy, Electron, Scanning; Proteome; Metabolic Networks and Pathways; Acetyl-CoA C-Acetyltransferase; Quorum Sensing
PubMed: 37542577
DOI: 10.1007/s00253-023-12705-y -
Antimicrobial Agents and Chemotherapy Apr 2023Mycobacterium fortuitum represents one of the most clinically relevant rapid-growing mycobacterial species. Treatments are complex due to antibiotic resistance and to...
Mycobacterium fortuitum represents one of the most clinically relevant rapid-growing mycobacterial species. Treatments are complex due to antibiotic resistance and to severe side effects of effective drugs, prolonged time of treatment, and co-infection with other pathogens. Herein, we explored the activity of NITD-916, a direct inhibitor of the enoyl-ACP reductase InhA of the type II fatty acid synthase in Mycobacterium tuberculosis. We found that this compound displayed very low MIC values against a panel of M. fortuitum clinical strains and exerted potent antimicrobial activity against M. fortuitum in macrophages. Remarkably, the compound was also highly efficacious in a zebrafish model of infection. Short duration treatments were sufficient to significantly protect the infected larvae from M. fortuitum-induced killing, which correlated with reduced bacterial burdens and abscesses. Biochemical analyses demonstrated an inhibition of synthesis of mycolic acids. Resolving the crystal structure of the InhA in complex with NAD and NITD-916 confirmed that NITD-916 is a direct inhibitor of InhA. Importantly, single nucleotide polymorphism leading to a G96S substitution in InhA conferred high resistance levels to NITD-916, thus resolving its target in M. fortuitum. Overall, these findings indicate that NITD-916 is highly active against M. fortuitum both and and should be considered in future preclinical evaluations for the treatment of M. fortuitum pulmonary diseases.
Topics: Animals; Mycobacterium fortuitum; Zebrafish; Mycobacterium tuberculosis; Mycolic Acids; Oxidoreductases
PubMed: 36920188
DOI: 10.1128/aac.01607-22 -
La Tunisie Medicale May 2018The association achalasia and non tuberculous Mycobacteria lung infection is described in the literature. Most of the time Mycobacterium Fortuitum is responsible of...
The association achalasia and non tuberculous Mycobacteria lung infection is described in the literature. Most of the time Mycobacterium Fortuitum is responsible of aspiration pneumonia that didn't respond to usual antibiotic therapy. We report a new case about a 15 year-old woman with Allgrove's syndrome history. The chest imaging showed a right pulmonary condensation and the diagnosis was bacteriological. Mycobacterium Fortuitum resistant to Rifampicin, isoniazid, Pyrazinamide and ethamabutol was isolated. She was treated by cotrimoxazole, ciprofloxacin and clarithromycin for 12 months, with a good clinical, radiological and bacteriological evolution. With the purpose to prevent the relapse the patient was treated by cardiomyotomy.
Topics: Adolescent; Adrenal Insufficiency; Anti-Bacterial Agents; Drug Resistance, Multiple, Bacterial; Esophageal Achalasia; Female; Humans; Mycobacterium Infections, Nontuberculous; Mycobacterium fortuitum
PubMed: 30430507
DOI: No ID Found -
American Journal of Infection Control Jul 2020Mycobacterium fortuitum survive in different environmental conditions, biofilm formation and resistance to chlorinated disinfectants makes its isolation frequent in...
BACKGROUND
Mycobacterium fortuitum survive in different environmental conditions, biofilm formation and resistance to chlorinated disinfectants makes its isolation frequent in hospital environments, even being involved in outbreaks by contamination of medical equipment such as bronchoscopes. We describe a pseudo-outbreak by M fortuitum isolated in samples from 9 patients who underwent bronchoscopy in the pneumology bronchoscopy unit of the University Hospital Complex of the Canary Islands from December 2016 to March 2017.
METHODS
We proceeded to investigate the pseudo-outbreak with a combination of epidemiologic, environmental, and molecular typing data.
RESULTS
The source/reservoir of pseudo-outbreak was the hospital water used by the bronchoscope automatic washing machine (without antibacterial filter), so control measures were taken. Molecular typing was performed on 7 strains from 7 patients, and a sample of water was collected from a tap in the pneumology bronchoscopy unit: all of which had the same pattern.
CONCLUSIONS
Our study demonstrates the presence of nontuberculous mycobacteria in the hospital water supply, and thus the need to take measures against them because they compromise patients' health. We also suggest the need for hospital water quality guidelines in which methods to control and/or eliminate them are established.
Topics: Bronchoscopy; Cross Infection; Disease Outbreaks; Equipment Contamination; Hospitals; Humans; Mycobacterium Infections, Nontuberculous; Mycobacterium fortuitum; Spain
PubMed: 31882175
DOI: 10.1016/j.ajic.2019.11.019