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Archives of Microbiology 1974
Comparative Study
Topics: Bacteria; Calcium; Cell Nucleus; Endoplasmic Reticulum; Glycogen; Inclusion Bodies; Lipids; Microscopy, Electron, Scanning; Mitochondria; Myxomycetes; Phagocytosis; Spores, Bacterial; Spores, Fungal; Vacuoles
PubMed: 4372966
DOI: 10.1007/BF00696247 -
Chemical & Pharmaceutical Bulletin May 2003Bahiensol (1), a new glycerolipid with antimicrobial activity has been isolated from a cultured plasmodium of myxomycete Didymium bahiense var. bahiense and its planar...
Bahiensol (1), a new glycerolipid with antimicrobial activity has been isolated from a cultured plasmodium of myxomycete Didymium bahiense var. bahiense and its planar structure was elucidated by spectral data.
Topics: Lipids; Magnetic Resonance Spectroscopy; Mass Spectrometry; Myxomycetes; Spectrometry, Mass, Fast Atom Bombardment; Spectrophotometry, Infrared
PubMed: 12736469
DOI: 10.1248/cpb.51.612 -
Journal of General Microbiology Oct 1970
Topics: Carbohydrate Metabolism; Carbohydrates; Chemotaxis; Fructose; Galactose; Glucose; Maltose; Mannose; Myxomycetes; Peptones; Ribose; Sucrose
PubMed: 5534501
DOI: 10.1099/00221287-63-2-221 -
Journal of Natural Products Oct 20106-Hydroxy-9'-methoxystaurosporinone (1), a new bisindole alkaloid, was isolated from field-collected fruiting bodies of the myxomycete Perichaena chrysosperma, together...
6-Hydroxy-9'-methoxystaurosporinone (1), a new bisindole alkaloid, was isolated from field-collected fruiting bodies of the myxomycete Perichaena chrysosperma, together with two known compounds. The structure of the new alkaloid was elucidated from spectral data, and compound 1 was shown to have hedgehog signal inhibitory activity. A related new alkaloid, 6,9'-dihydroxystaurosporinone (4), was also isolated from Arcyria cinerea.
Topics: Animals; Fruiting Bodies, Fungal; Humans; Indole Alkaloids; Japan; Molecular Structure; Myxomycetes; Nuclear Magnetic Resonance, Biomolecular
PubMed: 20839811
DOI: 10.1021/np1002687 -
Current Genetics Oct 1992We have examined five nuclear group I introns, located at three different positions in the large subunit ribosomal RNA (LSU rRNA) gene of the two myxomycete species,...
We have examined five nuclear group I introns, located at three different positions in the large subunit ribosomal RNA (LSU rRNA) gene of the two myxomycete species, Didymium iridis and Physarum polycephalum. Structural models of intron RNAs, including secondary and tertiary interactions, are proposed. This analysis revealed that the Physarum intron 2 contains an unusual core region that lacks the P8 segment, as well as several of the base-triples known to be conserved among group I introns. Structural and evolutionary comparisons suggest that the corresponding introns 1 and 2 were present in a common ancestor of Didymium and Physarum, and that the five introns in LSU rRNA genes of these myxomycetes were acquired in three different events. Evolutionary relationships, inferred from the sequence analysis of several different nuclear group I introns and the ribosomal RNA genes of the intron-harbouring organisms, strongly support horizontal transfer of introns in the course of evolution. We propose a model that may explain how myxomycetes in natural environments obtained their nuclear group I introns.
Topics: Animals; Base Sequence; Conserved Sequence; DNA, Fungal; DNA, Ribosomal; Introns; Models, Genetic; Molecular Sequence Data; Myxomycetes; Phylogeny; Physarum; RNA Splicing
PubMed: 1394512
DOI: 10.1007/BF00317925 -
Mycologia 2011A new species of Didymium (Myxomycetes), D. operculatum, is described in this paper, and details of its life cycle are provided. The new species was recorded during...
A new species of Didymium (Myxomycetes), D. operculatum, is described in this paper, and details of its life cycle are provided. The new species was recorded during studies of the Atacama Desert in Chile. It has been collected directly in the field and isolated in moist chamber cultures prepared with material from an endemic cactus. The distinguishing characters of this species are its dehiscence by means of an apical operculum combined with a whitish calcareous stalk and the banded reticulate ornamentation of the spores. The morphology of this new myxomycete was examined with scanning electron microscopy and light microscopy, and micrographs of relevant details are included in this paper. Some comments are made on the patterns of distribution of Didymium species in arid lands and adaptive characters enabling this genus to colonize such extreme environments. It is proposed that a longer cycle and the ability to resort to resistant forms many times during their development reflect the response of these myxomycetes to the largely unfavorable conditions of their environment.
Topics: Biodiversity; Chile; Desert Climate; Microscopy, Electron, Scanning; Morphogenesis; Mycology; Myxomycetes; Spores, Protozoan
PubMed: 21262984
DOI: 10.3852/10-363 -
CRC Critical Reviews in Microbiology Mar 1976
Review
Topics: Cell Wall; Cytoplasmic Streaming; Light; Myxomycetes; Physarum; Pigments, Biological; Pinocytosis; Spores, Fungal; Temperature
PubMed: 767055
DOI: 10.3109/10408417609106943 -
Journal of Bacteriology Apr 1969The plasmodial phase of the myxomycete Physarum rigidum, analyzed during the period of rapid growth, attained a ribonucleic acid (RNA) and protein content of 9.8 and...
The plasmodial phase of the myxomycete Physarum rigidum, analyzed during the period of rapid growth, attained a ribonucleic acid (RNA) and protein content of 9.8 and 60.0%, respectively, on a dry weight basis. It possessed ribosomes of the 80S class which, especially in the absence of magnesium ions, partially dissociated to 60S and 40S subunit classes. Electron micrographs of ribosomes treated with uranyl acetate-lead citrate revealed a number of surface features. Nucleotide analyses of both ribosomal and total RNA disclosed that they were composed of 51.0 and 52.5% guanylic and cytidylic acids, respectively. Consistent with most reports on other organisms, guanylic acid was the most abundant nucleotide found in the various types of RNA and cytidylic acid was the least abundant. The S(0) (20,w) values of the total RNA classes, in 0.01 sodium acetate (pH 4.6) containing 0.10 m NaCl, were 5.2, 18.1, and 27.3 in S units. Changing the ionic environment of the RNA (0.017 molal potassium phosphate, pH 7.0, containing 0.01 m disodium ethylenediaminetetraacetate) resulted in a reduction of the S(0) (20,w) values to 4.2, 16.6, and 22.6 in S units, which is indicative of molecular conformational transitions. In general, the amino acid composition of the ribosomal proteins was similar to the data available on ribosomal proteins from other biological sources.
Topics: Amino Acids; Microscopy, Electron; Myxomycetes; Nucleotides; Proteins; RNA; Ribosomes; Ultracentrifugation
PubMed: 5813808
DOI: 10.1128/jb.98.1.249-255.1969 -
Journal of Bacteriology Jul 1972Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo... (Comparative Study)
Comparative Study
Myxamoebae of the acellular slime mold Physarum polycephalum have been cultured axenically in a soluble medium. The growth medium contains bovine serum albumin, embryo extracts, liver infusion broth, peptone, and glucose. Cell densities ranging from 3 x 10(6) to 5 x 10(6) cells/ml have been obtained with this medium. To date, myxamoebae have been serially transferred more than 100 times without deleterious effect.
Topics: Agar; Animals; Carbon Radioisotopes; Cell Count; Chick Embryo; Culture Media; Escherichia coli; Glucose; Microscopy, Phase-Contrast; Myxomycetes; Peptones; Protein Hydrolysates; Serum Albumin, Bovine; Tissue Extracts
PubMed: 4591478
DOI: 10.1128/jb.111.1.242-247.1972 -
Journal of Ultrastructure Research Oct 1967
Topics: Microscopy, Electron; Myxomycetes; Organoids
PubMed: 5625692
DOI: 10.1016/s0022-5320(67)90286-9