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Archiv Fur Mikrobiologie 1973
Topics: Carbon Dioxide; Carbon Isotopes; Cell Differentiation; Citric Acid Cycle; Cyclic AMP; Gluconates; Glucose; Glycolysis; Myxomycetes; Oxygen Consumption; Pentosephosphates; Stimulation, Chemical; Time Factors
PubMed: 4350874
DOI: 10.1007/BF00424971 -
Journal of Microbiological Methods May 2021Unlike fungi, which have a universally accepted barcode marker, universal primers still lack in myxomycetes. Typically, DNA barcode primers were designed based on...
Unlike fungi, which have a universally accepted barcode marker, universal primers still lack in myxomycetes. Typically, DNA barcode primers were designed based on comparing existing myxomycetes sequences and targeting the conserved regions. However, the extreme genetic diversity within major myxomycetes groups and the frequent occurrence of group I introns have made the development of universal DNA barcode a severe challenge. The emergence of next-generation sequencing provides an opportunity to address this problem. We sequenced the mixed genomic DNA of 81 myxomycetes and extracted the SSU gene's reads using next-generation sequencing. After alignment and assembly, we designed a set of SSU primers that matched all potential SNPs, avoided all known group I intron insertion sites, and were highly conserved between major myxomycetes orders. This set of SSU primers has the potential to become one of the universal primer combinations. Due to the high genetic divergence caused by long and complicated evolutionary histories, the lack of universal barcode primers is common in protists. Our research provides a new method to solve this problem.
Topics: DNA Primers; Fungal Proteins; Genetic Variation; High-Throughput Nucleotide Sequencing; Myxomycetes; Phylogeny
PubMed: 33722637
DOI: 10.1016/j.mimet.2021.106203 -
Biochimica Et Biophysica Acta Apr 1968
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Calcium; Chemical Precipitation; Edetic Acid; Magnesium; Methods; Microscopy, Electron; Muscle Proteins; Myxomycetes; Proteins; Solubility; Ultracentrifugation; Viscosity
PubMed: 4231303
DOI: 10.1016/0005-2795(68)90011-1 -
Journal of Visualized Experiments : JoVE Nov 2017Our research is aimed at gaining a better understanding of the electronic properties of organisms in order to engineer novel bioelectronic systems and computing...
Our research is aimed at gaining a better understanding of the electronic properties of organisms in order to engineer novel bioelectronic systems and computing architectures based on biology. This specific paper focuses on harnessing the unicellular slime mold Physarum polycephalum to develop bio-memristors (or biological memristors) and bio-computing devices. The memristor is a resistor that possesses memory. It is the 4th fundamental passiveĀ circuit element (the other three are the resistor, the capacitor, and the inductor), which is paving the way for the design of new kinds of computing systems; e.g., computers that might relinquish the distinction between storage and a central processing unit. When applied with an AC voltage, the current vs. voltage characteristic of a memristor is a pinched hysteresis loop. It has been shown that P. polycephalum produces pinched hysteresis loops under AC voltages and displays adaptive behavior that is comparable with the functioning of a memristor. This paper presents the method that we developed for implementing bio-memristors with P. polycephalum and introduces the development of a receptacle to culture the organism, which facilitates its deployment as an electronic circuit component. Our method has proven to decrease growth time, increase component lifespan, and standardize electrical observations.
Topics: Biomimetics; Electric Impedance; Electrophysiological Phenomena; Myxomycetes
PubMed: 29155754
DOI: 10.3791/56076 -
Canadian Journal of Microbiology Oct 1999Axenic culture of myxomycete plasmodia has been attempted from time to time by various authors, but with very little success. From over 500 known species of myxomycetes,...
Axenic culture of myxomycete plasmodia has been attempted from time to time by various authors, but with very little success. From over 500 known species of myxomycetes, fewer than 20 species have been reported in axenic culture to date, including axenic myxamoebal cultures. In these cultures, the plasmodia required either complex media, or a killed bacterial supplement for growth. Furthermore, the time required for attaining the axenic state varied from several months to years. In the present study, a simple, rapid procedure has been developed to render monoxenic plasmodial cultures axenic. This procedure is based on our discovery that plasmodia have certain unusual substrate preferences that are inhibitory to the associated bacteria using Physarella oblonga as a model. The presence or absence of the bacteria could be ascertained through incubation in four different bacteriological media and by the use of a differential staining technique.
Topics: Animals; Bacteria; Culture Media; Myxomycetes; Parasitology
PubMed: 10907423
DOI: 10.1139/w99-080 -
Non-mendelian inheritance of mitochondrial DNA and ribosomal DNA in the myxomycete, Didymium iridis.Molecular & General Genetics : MGG Aug 1988The inheritance of both the mitochondrial DNA (mtDNA) and the nuclear-encoded extrachromosomal ribosomal DNA (rDNA) has been studied in the myxomycete, Didymium iridis,...
The inheritance of both the mitochondrial DNA (mtDNA) and the nuclear-encoded extrachromosomal ribosomal DNA (rDNA) has been studied in the myxomycete, Didymium iridis, by DNA-DNA hybridization of labeled probes to total DNA at various stages of the life cycle. Both the mtDNA and rDNA populations rapidly become homogeneous in individuals, but there is a qualitative difference in the patterns of inheritance of these two molecules. One parental rDNA type was preferentially inherited in all crosses; selective replication of this molecule is tentatively proposed as the mechanism of inheritance. In contrast, either parental mtDNA type could be inherited. Since the inherited population of parental mtDNA molecules are not partitioned into cells in this coenocytic organism, no known mechanism of inheritance can explain the rapid and apparently random loss of one parental mtDNA type in individuals.
Topics: Cloning, Molecular; DNA, Fungal; DNA, Mitochondrial; DNA, Ribosomal; Extrachromosomal Inheritance; Haploidy; Myxomycetes; Zygote
PubMed: 3185507
DOI: 10.1007/BF00339605 -
Developmental Biology Oct 1973
Topics: Cell Nucleus; Chromosomes; DNA; Fungal Proteins; Mitosis; Myxomycetes; Spores, Fungal
PubMed: 4799477
DOI: 10.1016/0012-1606(73)90352-7 -
Mycologia 1967
Topics: Myxomycetes
PubMed: 6068268
DOI: No ID Found -
Archives of Microbiology Mar 1975The haploid phase (myxamoebae-swarm cells) of the myxomycete Physarum flavicomum grew readily in chemical defined liquid media. The minimal medium contained salts,...
The haploid phase (myxamoebae-swarm cells) of the myxomycete Physarum flavicomum grew readily in chemical defined liquid media. The minimal medium contained salts, glucose, biotin, thiamine, hematin, glycine, L-arginine and L-methionine. Cell yields of 1.4 times 10(7) cells/ml were obtained in this medium in aerobic shake culture. These cells consumed about 35 muliters of oxygen/mg protein-hr in the minimal medium. The morphology of cells maintained in this medium appeared to be "normal". L-valine replaced either glycine or L-methionine in the minimal medium but the growth rates and cell yields were reduced. Growth rates increased in media containing four, seven, or fourteen amino acids.
Topics: Amino Acids; Biotin; Culture Media; Haploidy; Heme; Myxomycetes; Oxygen Consumption; Physarum; Thiamine
PubMed: 1171665
DOI: 10.1007/BF00428365 -
Bioorganic & Medicinal Chemistry Letters Jun 2005Two new bisindole alkaloids, 6-hydroxystaurosporinone (1) and 5,6-dihydroxyarcyriaflavin A (2) were isolated from field-collected fruit bodies of a myxomycete Lycogala...
Two new bisindole alkaloids, 6-hydroxystaurosporinone (1) and 5,6-dihydroxyarcyriaflavin A (2) were isolated from field-collected fruit bodies of a myxomycete Lycogala epidendrum, along with eight known bisindoles (3-10). The structures of these new compounds were determined on the basis of spectroscopic data. Compounds 1 and 2 showed cytotoxicity against HeLa, Jurkat, and vincristine resistant KB/VJ300 cells, and compound 1, particularly, inhibited protein tyrosine kinase activity.
Topics: Alkaloids; Animals; Enzyme Inhibitors; HeLa Cells; Humans; Magnetic Resonance Spectroscopy; Mice; Myxomycetes; NIH 3T3 Cells; Protein-Tyrosine Kinases
PubMed: 15911254
DOI: 10.1016/j.bmcl.2005.03.103