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Annals of Clinical Microbiology and... Mar 2022Olsenella uli is anaerobic or microaerophilic bacteria, commonly found in oral cavity or gastrointestinal tract, which has not been reported to be associated with lower...
BACKGROUND
Olsenella uli is anaerobic or microaerophilic bacteria, commonly found in oral cavity or gastrointestinal tract, which has not been reported to be associated with lower respiratory tract infection. Herein, we report the first case of Olsenella uli infection in the lung.
CASE PRESENTATION
A 70-year-old male farmer with no history of other respiratory tract diseases developed a cough with bloody sputum three times a day without obvious causes or other concomitant symptoms. After a period of treatment with empirical antibiotic, his condition did not improve. The computed tomography (CT) and lung biopsy results indicated bilateral pneumonia, and Olsenella uli was identified by micromorphology, sequence analysis and mass spectrometry analysis recovered from sputum. Ceftazidime, a third generation cephalosporin was used for the treatment, and the patient recovered after 10 days.
CONCLUSIONS
Our report suggests a causative role of gingival bacteria in the pathogenesis of pneumonia, thus early diagnosis and prompt antibiotic therapy may play a role in the treatment of Olsenella uli induced pneumonia.
Topics: Actinobacteria; Aged; Humans; Lung; Male; Pneumonia, Bacterial; RNA, Ribosomal, 16S
PubMed: 35232448
DOI: 10.1186/s12941-022-00499-2 -
International Journal of Systematic and... Apr 2011Strain A2 is an anaerobic, variably Gram-stain-positive, non-spore-forming, small and irregularly rod-shaped bacterium from the ruminal fluid of a sheep that has been...
Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa.
Strain A2 is an anaerobic, variably Gram-stain-positive, non-spore-forming, small and irregularly rod-shaped bacterium from the ruminal fluid of a sheep that has been described informally as a representative of 'Olsenella (basonym Atopobium) oviles'. Three phenotypically similar bacterial strains (lac15, lac16 and lac31(T)) were isolated in concert with Veillonella magna lac18(T) from the mucosal jejunum of a pig. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strains A2, lac15, lac16 and lac31(T) formed a genetically coherent group (100 % interstrain sequence similarity) within the bigeneric Olsenella-Atopobium branch of the family Coriobacteriaceae, class Actinobacteria. This group was most closely related to the type strains of the two recognized Olsenella species, namely Olsenella uli (sequence similarity of 96.85 %) and Olsenella profusa (sequence similarity of 97.20 %). The sequence similarity to the type strain of Atopobium minutum, the type species of the genus Atopobium, was 92.33 %. Unlike those of O. uli and O. profusa, outgrown colonies of strains A2, lac15, lac16 and lac31(T) were opaque and greyish-white with an umbonate elevation on solid culture media. The four novel strains were characterized as being well-adapted and presumably indigenous to the gastrointestinal tract of homoeothermic vertebrates: they were mesophilic, microaerotolerant, neutrophilic and acidotolerant, bile-resistant, mucin-utilizing and markedly peptidolytic lactic acid bacteria. The results of DNA-DNA hybridizations, cellular fatty acid analysis and other differential phenotypic (physiological and biochemical) tests confirmed that strains A2, lac15, lac16 and lac31(T) represent a novel species of the genus Olsenella. On the basis of the genotypic and phenotypic results, we therefore describe Olsenella umbonata sp. nov., with lac31(T) ( = CCUG 58604(T) = DSM 22620(T) = JCM 16156(T)) as the type strain and A2 ( = CCUG 58212 = DSM 22619 = JCM 16157) as an additionally available reference strain. Also, based on our data, we propose emended descriptions of the genus Olsenella and the species Olsenella uli and Olsenella profusa.
Topics: Actinobacteria; Anaerobiosis; Animals; Bacterial Typing Techniques; Cluster Analysis; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Jejunum; Lactic Acid; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S; Rumen; Sequence Analysis, DNA; Sheep; Swine
PubMed: 20435744
DOI: 10.1099/ijs.0.022954-0 -
International Journal of Systematic and... Sep 2001The diversity of organisms present in the subgingival pockets of patients with periodontitis and acute necrotizing ulcerative gingivitis (ANUG) were examined previously....
Characterization of novel human oral isolates and cloned 16S rDNA sequences that fall in the family Coriobacteriaceae: description of olsenella gen. nov., reclassification of Lactobacillus uli as Olsenella uli comb. nov. and description of Olsenella profusa sp. nov.
The diversity of organisms present in the subgingival pockets of patients with periodontitis and acute necrotizing ulcerative gingivitis (ANUG) were examined previously. The 16S rRNA genes of subgingival plaque bacteria were amplified using PCR with a universal forward primer and a spirochaete-selective reverse primer. The amplified DNA was cloned into Escherichia coli. In one subject with ANUG, 70 clones were sequenced. Seventy-five per cent of the clones were spirochaetal, as expected. Twelve of the remaining clones fell into two clusters that represent novel phylotypes in the family Coriobacteriaceae. The first novel phylotype was most closely related to Atopobium rimae (98% similarity). The phylotype probably represents a novel Atopobium species, but will not be named until cultivable strains are obtained. The second novel phylotype was only 91% similar to described Atopobium species and 84% similar to Coriobacterium glomerans. The 16S rRNA sequences of the type strain of Lactobacillus uli and a strain representing the Moores' Eubacterium group D52 were determined as part of on ongoing sequence analysis of oral bacteria. The sequence for L. uli was more than 99.8% similar to sequences for the second clone phylotype. It therefore appears that the second clone phylotype and L. uli represent the same species. The sequence for the Eubacterium D52 strain was 95.6% similar to that of L. uli. The G+C content of the DNA of L. uli and Eubacterium D52 is 63-64 mol %. These organisms are thus distinct from the neighbouring genus Atopobium, which has a DNA G+C content of 35-46 mol%. A new genus, Olsenella gen. nov., is proposed for these two species on the basis of phenotypic characteristics and 16S rRNA sequence analysis to include Olsenella uli comb. nov. and Olsenella profusa sp. nov.
Topics: Actinobacteria; Cloning, Molecular; DNA, Ribosomal; Dental Plaque; Genes, rRNA; Gingiva; Humans; Lactobacillus; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 11594611
DOI: 10.1099/00207713-51-5-1797 -
International Journal of Systematic and... Aug 2019A novel actinobacterial strain, designated KGMB04489, was isolated from the faeces of a healthy Korean. Cells of the strain were strictly anaerobic, Gram-stain-positive...
A novel actinobacterial strain, designated KGMB04489, was isolated from the faeces of a healthy Korean. Cells of the strain were strictly anaerobic, Gram-stain-positive and short-rod-shaped. On the basis of 16S rRNA gene sequence similarity, strain KGMB04489 belonged to the genus Olsenella and was most closely related to Olsenella scatoligenes SK9K4 (94.3 %), Olsenella uli ATCC 49627 (93.5 %), Olsenella umbonata lac31 (93.4 %) and Olsenella profusa D315A-29 (93.3 %). The major end product was lactic acid. The DNA G+C content was 65.5 mol%. The major cellular fatty acids of strain KGMB04489 were C18 : 1cis9, C18 : 1cis9 DMA and C16 : 0. Strain KGMB04489 contained meso-diaminopimelic acid as the diamino acid in the peptidoglycan. The polar lipids consisted of an unidentified phospholipid, six unidentified glycolipids and an unidentified lipid. Based on phylogenetic, physiological and chemotaxonomic characteristics, strain KGMB04489 is considered to represent a novel species within the genus Olsenella, for which the name Olsenellafaecalis sp. nov. is proposed. The type strain is KGMB04489 (=KCTC 15699=CCUG 72345).
Topics: Actinobacteria; Bacterial Typing Techniques; Base Composition; Cell Wall; DNA, Bacterial; Diaminopimelic Acid; Fatty Acids; Feces; Glycolipids; Humans; Peptidoglycan; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Republic of Korea; Sequence Analysis, DNA
PubMed: 31135332
DOI: 10.1099/ijsem.0.003469 -
Standards in Genomic Sciences Aug 2010Olsenella uli (Olsen et al. 1991) Dewhirst et al. 2001 is the type species of the genus Olsenella, which belongs to the actinobacterial family Coriobacteriaceae. The...
Olsenella uli (Olsen et al. 1991) Dewhirst et al. 2001 is the type species of the genus Olsenella, which belongs to the actinobacterial family Coriobacteriaceae. The species is of interest because it is frequently isolated from dental plaque in periodontitis patients and can cause primary endodontic infection. The species is a Gram-positive, non-motile and non-sporulating bacterium. The strain described in this study was isolated from human gingival crevices. This is the first completed sequence of the genus Olsenella and the fifth sequence from a member of the family Coriobacteriaceae. The 2,051,896 bp long genome with its 1,795 protein-coding and 55 RNA genes is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
PubMed: 21304694
DOI: 10.4056/sigs.1082860 -
International Journal of Systematic and... Dec 2021A novel Gram-stain-positive, strictly anaerobic, elliptical, non-motile and non-flagellated bacterium, designed LZLJ-2, was isolated from the mud in a fermentation...
A novel Gram-stain-positive, strictly anaerobic, elliptical, non-motile and non-flagellated bacterium, designed LZLJ-2, was isolated from the mud in a fermentation cellar used for the production of Chinese Luzhou-flavour Baijiu. Growth occurred at 28-45 °C (optimum, 37 °C), at pH 6.0-7.0 (optimum, pH 6.0) and with concentrations of NaCl up to 2 % (w/v; optimum, 0 %). On the basis of 16S rRNA gene sequence similarity, strain LZLJ-2 belonged to the genus and was most closely related to Marseille-P3256 (similarity 96.9 %), ClaCZ62 (similarity 96.6 %) and Marseille-P2912 (similarity 96.4 %). In addition, strain LZLJ-2 had high similarity to the genus , including DSM 13989 (similarity 94.9 %), DSM 22620 (similarity 94.9 %), ATCC 49627 (similarity 94.22 %), DSM 28304 (similarity 93.9 %) and KCTC 15699 (similarity 93.25 %). Comparative genome analysis showed that orthoANI values between strain LZLJ-2 and Marseille-P3256, ClaCZ62, Marseille-P2912, DSM 13989, DSM 22620, ATCC 49627, DSM 28304 and KCTC 15699 were 78.68, 78.99, 78.29, 73.40, 74.00, 74.30, 75.08 and 77.23 %, and the genome-to-genome distance values were respectively 22.3, 22.5, 22.4, 19.6, 20.5, 19.7, 20.5 and 21.5 %. The genomic DNA G+C content of strain LZLJ-2 was 65.21 mol%. The predominant cellular fatty acids (>10 %) of strain LZLJ-2 were C 9 (33.7 %), C (22.0 %) and C 9 DMA (13.5 %). d-Glucose, sucrose, mannose, maltose, lactose (weak), salicin, glycerol (weak), cellobiose and trehalose (weak) could be used by strain LZLJ-2 as sole carbon sources. Enzyme activity results showed positive reactions with valine arylamidase, leucine arylamidase, crystine arylamidase, acid phosphatase, alkaline phosphatase, esterase (C4) (weakly positive), naphthol-AS-BI-phosphohydrolase, -glucosidase and -glucosidase. The major end products of glucose fermentation were lactic acid and acetic acid. It produced skatole from indole acetic acid, and produced -cresol from modified peptone-yeast extract medium with glucose. Based on the 16S rRNA gene trees as well as the genome core gene tree, it is suggested that are transferred to genus as comb. nov. Based on phenotypic, genotypic and phylogenetic data, strain LZLJ-2 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LZLJ-2 (=KCTC 25162=JCM 34224).
Topics: Actinobacteria; Alcoholic Beverages; Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial; Fatty Acids; Fermentation; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology
PubMed: 34914571
DOI: 10.1099/ijsem.0.005192 -
International Journal of Systematic and... Apr 2015Strain SK9K4(T), which is a strictly anaerobic, non-motile, non-sporulating, Gram-stain-positive, saccharolytic coccobacillus, was isolated from pig faeces. SK9K4(T)...
Strain SK9K4(T), which is a strictly anaerobic, non-motile, non-sporulating, Gram-stain-positive, saccharolytic coccobacillus, was isolated from pig faeces. SK9K4(T) metabolized indol-3-acetic acid to 3-methylindole (skatole), which is the main contributor to boar taint; it also produced 4-methylphenol (p-cresol) from p-hydroxyphenylacetic acid. Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that the isolate represented a new lineage within the genus Olsenella of the family Atopobiaceae . Strain SK9K4(T) was most closely related to the type strains of the three species of the genus Olsenella with validly published names; Olsenella profusa DSM 13989(T) (93.6%), Olsenella uli DSM 7084(T) (93.5%) and Olsenella umbonata DSM 22620(T) (92.7%). DNA-DNA relatedness values of strain SK9K4(T) with O. profusa , O. uli and O. umbonata were 28.3%, 69.1% and 27.2%, respectively. The genomic DNA G+C content was 62.1 mol% and the major cellular fatty acids (constituting >10% of the total) were C(14 : 0) and C(18 : 1)ω9c. The major end product of glucose fermentation was lactic acid, with minor amounts of acetic acid and formic acid; no H2 was produced. Discrepancies in the fatty acid profiles, the MALDI-TOF mass spectra of cell extracts and the physiological and biochemical characteristics differentiated strain SK9K4(T) from other species of the genus Olsenella and indicate that the isolate represents a novel species within this genus. The name Olsenella scatoligenes sp. nov., is proposed and the type strain is SK9K4(T) ( = JCM 19907(T) = DSM 28304(T)).
Topics: Actinobacteria; Animals; Bacterial Typing Techniques; Base Composition; Cresols; DNA, Bacterial; Fatty Acids; Feces; Fermentation; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Skatole; Sus scrofa
PubMed: 25634945
DOI: 10.1099/ijs.0.000083 -
Letters in Applied Microbiology 2005To investigate the occurrence of Olsenella uli and Olsenella profusa in samples taken from infections of endodontic origin using a devised 16S RNA gene-based nested PCR...
AIMS
To investigate the occurrence of Olsenella uli and Olsenella profusa in samples taken from infections of endodontic origin using a devised 16S RNA gene-based nested PCR protocol.
METHODS AND RESULTS
DNA extracted from clinical samples was initially amplified using universal 16S rRNA gene primers followed by a second round of amplification using the first PCR products to detect a specific fragment of either O. uli or O. profusa 16S rRNA gene. Olsenella uli was detected in 27% of samples from teeth with asymptomatic periradicular lesions and in 20% of pus samples taken from symptomatic (abscess) lesions. Olsenella profusa was detected in 7% of asymptomatic teeth and in 13% of symptomatic teeth. A new Olsenella phylotype was also detected.
CONCLUSIONS
Olsenella species can take part in the microbiota associated with infections of the root canal of human teeth and a role in the pathogenesis of periradicular diseases is suspected.
SIGNIFICANCE AND IMPACT OF THE STUDY
The present study demonstrates that molecular genetic methods keep expanding the list of candidate endodontic pathogens to include Olsenella species.
Topics: Actinobacteria; Adult; Aged; Aged, 80 and over; DNA Primers; Humans; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; Pulpitis; RNA, Bacterial; RNA, Ribosomal, 16S; Species Specificity
PubMed: 15960746
DOI: 10.1111/j.1472-765X.2005.01723.x -
Clinical & Experimental Ophthalmology Nov 2008
Topics: Actinobacteria; Adult; Endophthalmitis; Humans; Leuconostoc; Male
PubMed: 19128392
DOI: 10.1111/j.1442-9071.2008.01890.x -
Journal of Endodontics May 2024In this study, we used metatranscriptomics for the first time to investigate microbial composition, functional signatures, and antimicrobial resistance (AR) gene...
INTRODUCTION
In this study, we used metatranscriptomics for the first time to investigate microbial composition, functional signatures, and antimicrobial resistance (AR) gene expression in endodontic infections.
METHODS
Root canal samples were collected from ten teeth, including five primary and five persistent/ secondary endodontic infections. RNA from endodontic samples was extracted, and RNA sequencing was performed on a NovaSeq6000 system (Illumina). Taxonomic analysis was performed using the Kraken2 bacterial database. Then, sequences with a taxonomic classification were annotated against the Universal Protein Knowledgebase for functional annotation and the Comprehensive Antibiotic Resistance Database for AR-like gene identification.
RESULTS
Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria represented the dominant phyla, whereas Fusobacteria, Spirochaetes, and Synergistetes were among the non-dominant phyla. The top ten species were mainly represented by obligate (or quasi-obligate) anaerobes, including Gram-negative (e.g., Capnocytophaga sp. oral taxon 323, Fusobacterium nucleatum, Prevotella intermedia, Prevotella oris, Tannerella forsythia and Tannerella sp. oral taxon HOT-286) and Gram-positive species (e.g., Olsenella uli and Parvimonas micra). Transcripts encoding moonlighting proteins (e.g., glycolytic proteins, translational elongation factors, chaperonin, and heat shock proteins) were highly expressed, potentially affecting bacterial adhesion, biofilm formation, host defense evasion, and inflammation induction. Endodontic bacteria expressed genes conferring resistance to antibiotic classes commonly used in dentistry, with a high prevalence and expression of tetracycline and lincosamide resistance genes. Antibiotic efflux and antibiotic target alteration/ protection were the main resistance mechanisms.
CONCLUSIONS
Metatranscriptomics revealed the activity of potential endodontic pathogens, which expressed putative virulence factors and a wide diversity of genes potentially involved in antimicrobial resistance.
PubMed: 38719087
DOI: 10.1016/j.joen.2024.03.015