-
Enzyme 1983An assay for oxalate, based on oxalate oxidase, was studied. It is relatively simple, rapid and reproducible and may be semi-automated and is accurate in the range...
An assay for oxalate, based on oxalate oxidase, was studied. It is relatively simple, rapid and reproducible and may be semi-automated and is accurate in the range 10-140 mg/l, has a coefficient of variation of 4.2% in the healthy range (10-19 mg/l serum). Samples may be stored at -20 degrees C for as long as 2 weeks. It cannot be used for the determination of urinary oxalate because of the presence of variable amounts of low molecular weight inhibitor(s).
Topics: Autoanalysis; Humans; Oxalates; Oxidoreductases; Reference Values; Spectrophotometry
PubMed: 6653548
DOI: 10.1159/000469589 -
Zeitschrift Fur Urologie Und Nephrologie Aug 1979It is reported on individual methods for the estimation of the oxalic acid in body fluids, particularly in the urine. The case in question is a survey of the oxalate... (Review)
Review
It is reported on individual methods for the estimation of the oxalic acid in body fluids, particularly in the urine. The case in question is a survey of the oxalate estimation methods, which, however, has no pretensions to completeness. The at present most actualestimation methods are brought somewhat more in detail. The data are not sufficient for the laboratorytechnical performance of the individual methods, this would transgress the possibilities of the work. However, the original papers are cited which contain all the necessary details. Some technical difficulties and disturbances in the individual estimation methods are also entered. Despite excellent work of several teams the problems of standardization, of the absolutely reliable reference methoda as well as of an objective consideration of advantages and disadvantages of individual, often subjectively judged methods is not yet solved. Comparing these methods, one gets the impression that several reliable methods of the same value are established. It seems that this estimation method brings the greatest progress which will reliably establish so small quantities of oxalate as they are in the blood or in the liquor. By this also the oxalate clearance and the renal oxalate treatment becomes more exactly establishable than up to now.
Topics: Carbon Radioisotopes; Chemical Precipitation; Humans; Hydrogen-Ion Concentration; Methods; Oxalates; Solubility; Urinary Calculi
PubMed: 41381
DOI: No ID Found -
Research in Microbiology 2003Oxalic acid and its salts are widespread in nature, as they are produced by many species of plants, algae and fungi. The bacteria, which are capable of using oxalate as... (Review)
Review
Oxalic acid and its salts are widespread in nature, as they are produced by many species of plants, algae and fungi. The bacteria, which are capable of using oxalate as a sole carbon and energy source, are described as being "oxalotrophic". Oxalotrophic bacteria do not constitute a homogeneous taxonomic group, but they do constitute a well-defined physiological group. A limited number of aerobic bacteria which are able to utilize oxalate as sole carbon and energy source have been completely described. Most of them are facultative methylotrophs and/or facultative hydrogen-oxidizing chemolithoautotrophs. In this review, the current status of the taxonomy and biodiversity of oxalotrophic bacteria in various environments, and aspects of their biotechnological potential, are briefly summarized.
Topics: Bacteria; Ecosystem; Oxalates; Phylogeny
PubMed: 12892846
DOI: 10.1016/S0923-2508(03)00112-8 -
Acta Crystallographica. Section C,... Jan 2001In the title compound, C2H6NO2+.C2HO4-, the glycine molecule exists in the cationic form and the oxalic acid molecule in the mono-ionized state. The molecules aggregate...
In the title compound, C2H6NO2+.C2HO4-, the glycine molecule exists in the cationic form and the oxalic acid molecule in the mono-ionized state. The molecules aggregate into alternate columns of glycinium and semi-oxalate ions. The structure is stabilized by an extensive network of hydrogen bonds.
Topics: Crystallography, X-Ray; Glycine; Hydrogen Bonding; Models, Molecular; Molecular Conformation; Oxalates
PubMed: 11173420
DOI: 10.1107/s0108270100015456 -
Acta Crystallographica. Section C,... Jun 2008The title salt, C3H8NO2+.C2HO4-, formed between L-cysteine and oxalic acid, was studied as part of a comparison of the structures and properties of pure amino acids and...
The title salt, C3H8NO2+.C2HO4-, formed between L-cysteine and oxalic acid, was studied as part of a comparison of the structures and properties of pure amino acids and their cocrystals. The structure of the title salt is very different from that formed by oxalic acid and equivalent amounts of D- and L-cysteine molecules. The asymmetric unit contains an L-cysteinium cation and a semioxalate anion. The oxalate anion is only singly deprotonated, in contrast with the double deprotonation in the crystal structure of bis(DL-cysteinium) oxalate. The oxalate anion is not planar. The conformation of the L-cysteinium cation differs from that of the neutral cysteine zwitterion in the monoclinic and orthorhombic polymorphs of L-cysteine, but is similar to that of the cysteinium cation in bis(DL-cysteinium) oxalate. The structure of the title salt can be described as a three-dimensional framework formed by ions linked by strong O-H...O and N-H...O and weak S-H...O hydrogen bonds, with channels running along the crystallographic a axis containing the bulky -CH2SH side chains of the cysteinium cations. The cations are only linked through hydrogen bonds via semioxalate anions. There are no direct cation-cation interactions via N-H...O hydrogen bonds between the ammonium and carboxylate groups, or via weaker S-H...S or S-H...O hydrogen bonds.
Topics: Crystallography, X-Ray; Cysteine; Hydrogen Bonding; Molecular Structure; Oxalates
PubMed: 18535345
DOI: 10.1107/S0108270108014911 -
Contributions To Nephrology 1997
Review
Topics: Calcium Oxalate; Glyoxylates; Humans; Hyperoxaluria, Primary; Liver; Microbodies; Mutation; Oxalates; Oxalic Acid; Transaminases
PubMed: 9399058
DOI: 10.1159/000059893 -
Clinica Chimica Acta; International... Oct 1980A gas-chromatographic procedure for the analysis of oxalic acid is described. The procedure requires relatively small quantities of urine (1 ml) or plasma (5 ml). The...
A gas-chromatographic procedure for the analysis of oxalic acid is described. The procedure requires relatively small quantities of urine (1 ml) or plasma (5 ml). The procedure consists of three steps: (1) extraction of exalic acid by acidified diethyl ether; (2) esterification by isopropanol; and (3) final analysis by gas chromatography. The oxalic acid was found to have an elution temperature of 107 degrees C and a retention time of 14 min. The standard curve is linear up to 800 nmol. In the described condition the lower limit of detection is 20 nmol. The mean normal plasma and urine oxalate levels (mean +/- 2 S.D.) were found to be 20 mumol/l +/- 17.5 and 275 mumol/24 h +/- 200 respectively.
Topics: Chromatography, Gas; Humans; Oxalates
PubMed: 7418227
DOI: 10.1016/0009-8981(80)90311-3 -
The Journal of Urology Jan 1982Changes in oxalate excretion (together with changes in urinary volume) constitute the most important factors in altering the probability of renal stone formation.... (Review)
Review
Changes in oxalate excretion (together with changes in urinary volume) constitute the most important factors in altering the probability of renal stone formation. However, investigations on oxalate metabolism have been sparse, perhaps because of the lack of an accurate method for measuring oxalate in biologic fluids. Available data clearly implicate increased urinary oxalate excretion as the etiological factor in stone formation in two groups of patients--those with primary hyperoxaluria and those with gastrointestinal malabsorption. Evidence for the existence of hyperoxaluria in the patient with the "garden" variety of calcium oxalate stones is less persuasive.
Topics: Animals; Biological Transport; Calcium Oxalate; Diet; Gastrointestinal Diseases; Glyoxylates; Humans; Intestinal Absorption; Kidney Calculi; Oxalates; Oxalic Acid; Rats; Rats, Inbred Strains; Urinary Calculi
PubMed: 7035692
DOI: 10.1016/s0022-5347(17)53649-6 -
Journal of Applied Toxicology : JAT 1994
Review
Topics: Animals; Environmental Exposure; Female; Humans; Male; Oxalates; Oxalic Acid
PubMed: 8083486
DOI: 10.1002/jat.2550140315 -
American Journal of Kidney Diseases :... Mar 1982A 13-year-old boy with primary hyperoxaluria and a successful renal allograft developed symptomatic bone disease, hypercalcemia, and hypercalciuria. Transiliac bone...
A 13-year-old boy with primary hyperoxaluria and a successful renal allograft developed symptomatic bone disease, hypercalcemia, and hypercalciuria. Transiliac bone biopsy revealed calcium oxalate crystals in the marrow within mononuclear phagocytes and multinucleated giant cells. Deep resorption bays were seen adjacent to these crystal-cell aggregates. Serum 1,25-(OH)2-vitamin D (calcitriol) and iPTH concentrations were low or normal. We suggest that hypercalcemia results from macrophage-mediated bone resorption initiated by Ca oxalate crystal deposition.
Topics: Adolescent; Bone Diseases; Bone Resorption; Calcium Oxalate; Humans; Hypercalcemia; Kidney Transplantation; Male; Oxalates; Oxalic Acid
PubMed: 7041638
DOI: 10.1016/s0272-6386(82)80028-0