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Journal of Inherited Metabolic Disease 2005High lactate concentrations occur in type I glycogen storage disease (GSD) whenever glycogenolysis occurs. Not only does hyperlactataemia cause acute clinical...
High lactate concentrations occur in type I glycogen storage disease (GSD) whenever glycogenolysis occurs. Not only does hyperlactataemia cause acute clinical deterioration, but chronic lactate elevations have also been associated with many of the long-term complications in GSD. A portable finger-stick blood lactate meter has recently been marketed as a training tool for high-performance athletes, but it has not been tested as a clinical diagnostic tool. This study was performed to assess the accuracy of the portable lactate meter in subjects with GSD I who are predisposed to high lactate concentrations. A total of 166 intravenous and 39 capillary samples from 13 subjects were tested concomitantly on three different lactate meters. The meter readings were compared with the lactate concentration determined by the laboratory gold-standard enzymatic colorimetric assay. Almost no inter-meter variability was found. The lactate meter values had outstanding correlation with the laboratory lactate determination, although the meters were found to run 0.5 mmol/L higher than the laboratory assay. The meter deviation was independent of lactate concentration. More variability was noted with finger-stick capillary lactate determinations, but monitoring of trends with capillary samples should prove valuable as a method for determining long-term control or acute deterioration. The portable lactate meter is a highly accurate tool for monitoring lactate concentrations, and should prove valuable for monitoring metabolic control in patients with GSD type I and other disorders associated with hyperlactataemia.
Topics: Adolescent; Adult; Biochemistry; Chemistry, Clinical; Child; Child, Preschool; Colorimetry; Evaluation Studies as Topic; Female; Glucose; Glucose-6-Phosphatase; Glycogen; Glycogen Storage Disease Type I; Humans; Infant; Lactates; Lactic Acid; Male; Monitoring, Ambulatory; Regression Analysis; Reproducibility of Results
PubMed: 16151900
DOI: 10.1007/s10545-005-0090-1 -
Vascular Medicine (London, England) Feb 2013Propionyl-L-carnitine (PLC) may improve exercise performance in patients with peripheral artery disease, but results from clinical trials have been inconsistent. The... (Meta-Analysis)
Meta-Analysis Review
Propionyl-L-carnitine (PLC) may improve exercise performance in patients with peripheral artery disease, but results from clinical trials have been inconsistent. The safety and efficacy of PLC for treatment of claudication was evaluated by a systematic review and meta-analysis of clinical trials for which data were available through September 2010. Eighty-five studies were identified, of which 13 were randomized controlled trials. Owing to database availability for the six phase III studies carried out with PLC (1 g orally, twice daily), a patient-level meta-analysis was conducted as the primary analysis. Treadmill performance data from these six studies were harmonized to peak walking distance (PWD) on a 7% grade at a speed of 3 km/hour. PLC (n = 440) was associated with a net 16 meter improvement (95% CI, 8-20 meters) in PWD as compared with placebo (n = 427) in the primary analysis (p = 0.002). The effect of PLC was similar in subpopulations defined using clinical and demographic variables, with possible enhanced benefit in patients engaged in an exercise program or enrolled at study sites in Russia. The systematic review of the effect of PLCs on claudication identified seven additional randomized controlled trials for a total of 13 trials, which included 681 patients on placebo and 672 on PLC. This meta-analysis confirmed a 45 meter net improvement on PLC using a random-effects model. In conclusion, oral PLC is associated with a statistically significant increase in PWD in patients with claudication, which may be clinically relevant.
Topics: Carnitine; Clinical Trials as Topic; Exercise Test; Humans; Intermittent Claudication; Walking
PubMed: 23321261
DOI: 10.1177/1358863X12467491 -
Terapevticheskii Arkhiv Dec 2023A point-of-care glucose testing (POCT) is an essential component of care in patients with hyperglycemia and hypoglycemia in inpatient and outpatient settings. In Russian...
BACKGROUND
A point-of-care glucose testing (POCT) is an essential component of care in patients with hyperglycemia and hypoglycemia in inpatient and outpatient settings. In Russian medical facilities (MFs), conventional glucose meters designed for self-monitoring by patients with diabetes are commonly used for POCT. These home-use meters have two serious disadvantages: the first is large measurement bias and the second - they can't be integrated into laboratory information systems, so measurement data have to be recorded into patient charts manually. Both factors may lead to medical errors. It is reasonable to use in the MFs specialized POCT glucose meters, as they are superior to conventional ones in accuracy and may be easily connected to laboratory information systems. With this in mind, physicians at the Russian Children's Clinical Hospital decided to substitute conventional meters with the Accu-Chek Inform II POCT meter, however, after preliminary performance assessment of the model.
AIM
To test the Accu-Chek Inform II performance characteristics: accuracy, linearity, repeatability, and mean absolute relative difference (MARD).
MATERIALS AND METHODS
Performance of the Accu-Chek Inform II was tested by comparing the results of parallel C measurements with the meter and reference laboratory analyzer in capillary blood samples. Overall, 99 parallel C measurements were made in 45 samples. Accuracy was evaluated according to the ISO 15197-2013 and POCT12-A3 criteria.
RESULTS
The Accu-Chek Inform II meter met the requirements of ISO 15197-2013 and POCT12-A3 and demonstrated high linearity (correlation coefficient, =1,0), good repeatability (mean coefficient of variation, CV=1,38%) and acceptable MARD (4,9%).
CONCLUSION
The Accu-Chek Inform II POCT glucose meter may be efficiently and safely used in inpatient and outpatient MFs and particularly in pediatric clinics.
Topics: Humans; Blood Glucose; Blood Glucose Self-Monitoring; Russia; Point-of-Care Systems; Point-of-Care Testing; Reproducibility of Results; Diabetes Mellitus
PubMed: 38785055
DOI: 10.26442/00403660.2023.12.202522 -
Annals of Clinical Biochemistry Jan 2014To assess glucose meter performance in a diabetes antenatal clinic, focussing on clinical and pre-analytical factors that might impact on the quantification of meter...
BACKGROUND
To assess glucose meter performance in a diabetes antenatal clinic, focussing on clinical and pre-analytical factors that might impact on the quantification of meter accuracy and precision.
METHODS
The Freestyle Lite and the Performa glucose meters were assessed by trained researchers. Finger stick glucose was measured and compared with plasma venous glucose, obtained from a concomitantly collected antecubital fossa sample. Venous plasma was separated on-site then sent to the laboratory for measurement of glucose using the hexokinase method (comparative method). Additional data collected included: (i) timing of and also (ii) quantity of last carbohydrate intake; (iii) time periods between collection, preparation and analysis of the venous sample; (iv) the haemolysis index of the plasma sample and (v) haematocrit.
RESULTS
There were 104 participants. Both meters fulfilled ISO 15197 standards, with 99% and 97% of Freestyle Lite and Performa results, respectively, falling within acceptable limits for this standard. Both meters showed minor proportional bias, reading low at higher glucose values. Consensus error grid analysis showed 100% of results from the Freestyle Lite and 99% from the Performa falling within Zone A, thus the observed differences in measured capillary and venous plasma glucose were sufficiently minor that they would have little effect on clinical action. No association was observed between [capillary-plasma] glucose difference and the five variables outlined above.
CONCLUSIONS
The two glucose meters tested showed a reassuringly acceptable level of performance, when assessed by a research team in the setting of a diabetes antenatal clinic.
Topics: Blood Glucose; Blood Glucose Self-Monitoring; Clinical Laboratory Techniques; Diabetes, Gestational; Female; Humans; Pregnancy; Pregnancy Complications; Prenatal Diagnosis
PubMed: 24003257
DOI: 10.1177/0004563213487893 -
Trends in Analytical Chemistry : TRAC Mar 2020There is a growing demand for high-performance point-of-care (POC) diagnostic technologies where in vitro diagnostics (IVD) is fundamental for prevention,...
There is a growing demand for high-performance point-of-care (POC) diagnostic technologies where in vitro diagnostics (IVD) is fundamental for prevention, identification, and treatment of many diseases. Over the past decade, a shift of IVDs from the centralized laboratories to POC settings is emerging. In this review, we summarize recent progress in translating IVDs from centralized labs to POC settings using commercially available handheld meters. After introducing typical workflows for IVDs and highlight innovative technologies in this area, we discuss advantages of using commercially available handheld meters for translating IVDs from centralized labs to POC settings. We then provide comprehensive coverage of different signal transduction strategies to repurpose the commercially-available handheld meters, including personal glucose meter, pH meter, thermometer and pressure meter, for detecting a wide range of targets by integrating biochemical assays with the meters for POC testing. Finally, we identify remaining challenges and offer future outlook in this area.
PubMed: 32194293
DOI: 10.1016/j.trac.2019.115782 -
The Journal of Pediatrics Aug 1982The correlation of transcutaneous bilirubin measurements with serum bilirubin concentrations is not good enough to allow for accurate prediction of the serum values. To... (Comparative Study)
Comparative Study
The correlation of transcutaneous bilirubin measurements with serum bilirubin concentrations is not good enough to allow for accurate prediction of the serum values. To impose the jaundice meter's potential clinical usefulness, we evaluated 344 paired jaundice meter-serum bilirubin measurements in 125 infants, using new guidelines from the marketing company which were designed to identify which infants require serum bilirubin determinations rather than to predict the actual bilirubin values. Use of the new guidelines correctly assessed the need for serum determinations in most infants, but false positives and, more importantly, false negatives (missed high serum values) did occur.
Topics: Bilirubin; Equipment and Supplies; Ethnicity; False Negative Reactions; False Positive Reactions; Humans; Infant, Newborn; Jaundice, Neonatal; Skin; Skin Pigmentation
PubMed: 7097424
DOI: 10.1016/s0022-3476(82)80135-2 -
Journal of Hazardous Materials May 2000Changes in pH and temperature were monitored in two freshwater ponds in Southeastern Massachusetts from 1990 to 1993 using a remote-sensing system that collected data on...
Changes in pH and temperature were monitored in two freshwater ponds in Southeastern Massachusetts from 1990 to 1993 using a remote-sensing system that collected data on a continuous basis. The sensing system included a combination electrode, pH meter and portable computer powered by a marine battery. Temperature and pH information from the pH meter were acquired every 10 min and stored in the computer. The two ponds, located within 2 km of one another, have a different average pH and sensitivity to acid precipitation. Maquan Pond has an average pH of 6.0 and an alkalinity of 7.4 mg/l, while Furnace Pond has an average pH of 6.9 and alkalinity of 14.9 mg/l. The pH of both ponds varied seasonally and showed diel changes due to the photosynthetic and respiratory activity of aquatic organisms. Precipitation events did not change the pH of Furnace Pond. Maquan Pond on the other hand, did exhibit changes in surface water pH due to specific acidic precipitation events. During certain rainstorms, the pH of Maquan surface waters dropped to values as low as pH 4. In addition to the transient changes in pH, the acid-sensitive pond also exhibited differences in planktonic distribution patterns.
Topics: Acid Rain; Animals; Circadian Rhythm; Environmental Monitoring; Fresh Water; Hydrogen-Ion Concentration; Metabolism; Plankton; Rain; Seasons
PubMed: 10781722
DOI: 10.1016/s0304-3894(99)00203-4 -
Meat Science Feb 2004A patented method of rapidly determining the ultimate pH from approximate glycolytic potential of muscles of slaughtered animals has been devised. The method is based on...
A patented method of rapidly determining the ultimate pH from approximate glycolytic potential of muscles of slaughtered animals has been devised. The method is based on the rapid hydrolysis of muscle glycogen to glucose by the enzyme amyloglucosidase and subsequent measurement of the liberated glucose. In acetate buffer at pH 4.5, glucose concentration can be determined in 30 s with domestic meters for diabetes control. The meter response differed from that of glucose in blood, but was linear with concentration. In slurries comprising homogenised meat in acetate buffer and added glucose, a similar linear response was obtained. Amyloglucosidase was capable of rapidly hydrolysing glycogen to glucose in such slurries. In the 24 h following slaughter, a decrease in glycogen, as determined by glucose, occurred in parallel with the decline in pH. At the same time, lactate progressively accumulated as expected. Values for the approximate glycolytic potential and (by calibration) ultimate pH, were obtained on prerigor muscle within 7 min of muscle sampling in an industrial environment. The method is suitable for on-line application in beef abattoirs particularly those employing hot boning where ultimate must be known at the grading point.
PubMed: 22064153
DOI: 10.1016/S0309-1740(03)00140-2 -
Archives of Oral Biology Mar 1997A new system for measuring saliva pH by radio telemetry was developed. The system was built into a lower complete denture and could operate for about 19 h. The maximum... (Comparative Study)
Comparative Study
A new system for measuring saliva pH by radio telemetry was developed. The system was built into a lower complete denture and could operate for about 19 h. The maximum error in the range pH 5-8 was 0.15 pH, and the correlation when values were compared with those from a glass-electrode pH meter was 0.99. That this system operates successfully was confirmed in a clinical experiment.
Topics: Aged; Denture, Complete; Electrodes; Equipment Design; Female; Glass; Humans; Hydrogen-Ion Concentration; Saliva; Telemetry; Time Factors
PubMed: 9188996
DOI: 10.1016/s0003-9969(96)00119-7 -
Sensors (Basel, Switzerland) Nov 2019Site-specific liming helps increase efficiency in agricultural production. For adequate determination of the lime demand, a combination of apparent soil electrical...
Site-specific liming helps increase efficiency in agricultural production. For adequate determination of the lime demand, a combination of apparent soil electrical conductivity (EC) and topsoil pH can be used. Here, it was hypothesized that this can also be done at low-input level. Field measurements using the EM38 MK I (Geonics, Canada) were conducted on three experimental sites in north Germany in 2011. The topsoil pH was measured based on two approaches: on the field using a handheld pH meter (Spectrum-Technologies Ltd., Bridgend, UK) with a flat electrode (in situ), and in the lab using standard equipment (ex situ). Both soil EC (0.4-35.9 mS m) and pH (5.13-7.41) were heterogeneously distributed across the sites. The same was true of the lime demand (-1.35-4.18 Mg ha). There was a significant correlation between in situ and ex situ determined topsoil pH (r = 0.89; < 0.0001). This correlation was further improved through non-linear regression (r = 0.92; < 0.0001). Thus, in situ topsoil pH was found suitable for map-overlay with EC to determine the site-specific lime demand. Consequently, the hypothesis could be confirmed: The combined use of data from EM38 and handheld pH meters is a promising low-input approach that may help implement site-specific liming in developing countries.
PubMed: 31801215
DOI: 10.3390/s19235280