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Frontiers in Microbiology 2019Comparative analysis of partial , , and gene sequences of 84 reference strains and field isolates revealed several clusters that included no taxonomic reference...
Comparative analysis of partial , , and gene sequences of 84 reference strains and field isolates revealed several clusters that included no taxonomic reference strains. The , , and phylogenetic trees were used to select 27 strains for whole-genome sequence analysis and for a comparative genomics study that also included 41 publicly available genome sequences. The phylogenomic analyses included a Genome BLAST Distance Phylogeny approach to calculate pairwise digital DNA-DNA hybridization values and their confidence intervals, average nucleotide identity analyses using the OrthoANIu algorithm, and a whole-genome phylogeny reconstruction based on 107 single-copy core genes using bcgTree. These analyses, along with subsequent chemotaxonomic and traditional phenotypic analyses, revealed the presence of 17 novel species among the strains analyzed, and allowed the identification of several unclassified strains reported in the literature. The genus has an open pan genome that includes many orthogroups in the 'Xenobiotics biodegradation and metabolism' KEGG pathway, which likely explains the enrichment of these species in polluted soils and participation in the biodegradation of complex organic substances. We propose to formally classify the 17 novel species as sp. nov. (type strain LMG 31117 = CCUG 73385), sp. nov. (type strain LMG 31108 = CCUG 73386), sp. nov. (type strain LMG 31011 = CCUG 73384), sp. nov. (type strain LMG 20603 = ATCC BAA-110), sp. nov. (type strain LMG 20602 = ATCC BAA-109), sp. nov. (type strain LMG 31118 = CCUG 73387), sp. nov. (type strain LMG 31106 = CCUG 39680), sp. nov. (type strain LMG 31010 = CCUG 73378), sp. nov. (type strain LMG 31110 = CCUG 73383), sp. nov. (type strain LMG 31012 = CCUG 73380), sp. nov. (type strain LMG 31112 = CCUG 73379), sp. nov. (type strain LMG 31009 = CCUG 73377), sp. nov. (type strain LMG 31116 = CCUG 73389), sp. nov. (type strain LMG 31109 = CCUG 73390), sp. nov. (type strain LMG 31114 = CCUG 73388), sp. nov. (type strain LMG 31014 = CCUG 73382), and sp. nov. (type strain LMG 31013 = CCUG 73381).
PubMed: 31781066
DOI: 10.3389/fmicb.2019.02556 -
Journal of the Royal Society of Medicine 2003
Review
Topics: Betaproteobacteria; Burkholderia Infections; Burkholderia cepacia; Cross Infection; Cystic Fibrosis; Gram-Negative Bacterial Infections; Humans; Methicillin Resistance; Pseudomonas Infections; Respiratory Tract Infections; Staphylococcal Infections
PubMed: 12906328
DOI: No ID Found -
Frontiers in Microbiology 2016Pandoraea species are considered as emerging pathogens in people with cystic fibrosis (CF). The contribution of these organisms to disease progression in CF patients is...
Pandoraea species are considered as emerging pathogens in people with cystic fibrosis (CF). The contribution of these organisms to disease progression in CF patients is not fully understood owing in large measure to the scant reports in clinical and research literature describing their colonization of CF patients and their associated virulence determinants. In an effort to increase awareness and evidence for Pandoraea spp. infection in people with CF, and to stimulate research aimed at unraveling the pathogenic properties of Pandoraea, we report a case of a 26-year-old Australian (Tasmanian) man with CF who was chronically infected with Pandoraea pnomenusa for at least one year prior to his death from respiratory failure. In addition, we describe for the first time evidence suggesting that this bacterium is a facultative anaerobe and report on the availability of a whole genome sequence for this organism. To the best of our knowledge, this report represents only the second clinical case study of P. pnomenusa infection in the world, and the first in an Australian CF patient.
PubMed: 27242717
DOI: 10.3389/fmicb.2016.00692 -
Infection and Drug Resistance 2022() infection is of great concern as these gram-negative bacillus species are multidrug-resistant and usually isolated from the patients' respiratory tract suffering...
() infection is of great concern as these gram-negative bacillus species are multidrug-resistant and usually isolated from the patients' respiratory tract suffering from cystic fibrosis (CF). A few cases of infection have also been reported in non-CF patients due to its rare pathogenic nature with unclear and overlapping clinical, biochemical, and microbiological characteristics with other species. Here, we report an unusual case of a 46-year-old non-CF female, who presented with multiple pelvic fractures, acute traumatic brain injury, multiple rib fractures, and multiple burns (18% of the total body surface area, II°) by the collapse of a brick kiln, suffered from sepsis due to wound infection. Pandoraea species were isolated both from her blood and wound secretion. Antibiotic susceptibility testing indicated susceptibility to imipenem, tetracyclines, sulfamethoxazole, and ampicillin/sulbactam but resistance to meropenem, quinolones, aminoglycosides, and other beta-lactams. 16S ribosomal RNA (rRNA) PCR assays and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to confirm the bacteria as . After effective anti-infection of intravenous antibiotics (imipenem 1.0 Q8H with tigecycline 50 mg Q12H for 14 days), wound care, and other comprehensive treatment for two months, the patient improved and was discharged from the hospital eventually. After reviewing the literature, we observed that the susceptibility results of Pandoraea species were often multidrug-resistant and had a unique pattern of being resistant to meropenem but sensitive to imipenem. Biofilm formation, carbapenemase production, and unique gene procession differed from the environmental isolates could help explain its resistance. This case report highlights the potential virulence of as a pathogen in patients with no underlying disease. Although they are often multi-resistant, imipenem can be a preferred treatment for Pandoraea species in the earliest identification steps.
PubMed: 36483145
DOI: 10.2147/IDR.S388520 -
Journal of the Association of Medical... Oct 2020Diagnosis and clinical management of pulmonary infections in lung transplant patients are challenging. The increased diversity of bacterial species identified from...
Diagnosis and clinical management of pulmonary infections in lung transplant patients are challenging. The increased diversity of bacterial species identified from clinical samples with novel proteomics-based systems can further complicate clinical decision making in this highly vulnerable population. Whether newly recognized organisms are colonizers or true pathogens often remains controversial since symptoms causality and impact on lung function is often unknown. We present the case of a 48-year-old female lung transplant patient with sp infection. We review and discuss the role of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for accurate bacterial identification. We report on therapeutic management and clinical outcome.
PubMed: 36341313
DOI: 10.3138/jammi-2020-0001 -
New Microbes and New Infections Nov 2013Pandoraea are considered emerging multidrug resistant pathogens in the context of cystic fibrosis. We report herein for the first time the case of a 30-year-old woman...
Pandoraea are considered emerging multidrug resistant pathogens in the context of cystic fibrosis. We report herein for the first time the case of a 30-year-old woman with cystic fibrosis, living in France, who was chronically infected with Pandoraea pulmonicola and who died of Pseudomonas aeruginosa sepsis 3 weeks after bilateral lung transplantation.
PubMed: 25356323
DOI: 10.1002/2052-2975.16 -
Journal of Cystic Fibrosis : Official... May 2020A structured survey of the cystic fibrosis pathogens Achromobacter, Pandoraea and Ralstonia species from thirteen sentinel hospitals throughout England was undertaken by...
A structured survey of the cystic fibrosis pathogens Achromobacter, Pandoraea and Ralstonia species from thirteen sentinel hospitals throughout England was undertaken by Public Health England. One isolate per patient of these genera collected from CF patients during the seven-month survey period in 2015 was requested from participating hospitals. Species-level identification was performed using nrdA/gyrB sequence cluster analysis, and genotyping by pulsed-field gel electrophoresis. In total, 176 isolates were included in the survey; 138 Achromobacter spp. (78.4%), 29 Pandoraea spp. (16.5%) and 9 Ralstonia spp. (5.1%). Novel Achromobacter and Pandoraea clusters were identified. High levels of antimicrobial resistance were found, particularly among Pandoraea isolates. Genotyping analysis revealed considerable diversity, however one geographically-widespread cluster of A. xylosoxidans isolates from six hospitals was found, in addition to two other clusters, both comprising isolates from two hospitals, either derived from the same region (A. xylosoxidans), or from hospitals within the same city (P. apista).
Topics: Achromobacter denitrificans; Adult; Anti-Bacterial Agents; Burkholderiaceae; Child; Cross Infection; Cystic Fibrosis; Drug Resistance, Microbial; England; Epidemiological Monitoring; Female; Gram-Negative Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Multilocus Sequence Typing; Ralstonia; Respiratory Tract Infections
PubMed: 31862307
DOI: 10.1016/j.jcf.2019.11.005 -
International Journal of Systematic and... Sep 2011Five isolates, designated TA2, TA4, TA25(T), KOx(T) and NS15(T) were isolated in previous studies by enrichment in mineral medium with potassium oxalate as the sole...
Five isolates, designated TA2, TA4, TA25(T), KOx(T) and NS15(T) were isolated in previous studies by enrichment in mineral medium with potassium oxalate as the sole carbon source and were characterized using a polyphasic approach. The isolates were Gram-reaction-negative, aerobic, non-spore-forming rods. Phylogenetic analyses based on 16S rRNA and DNA gyrase B subunit (gyrB) gene sequences confirmed that the isolates belonged to the genus Pandoraea and were most closely related to Pandoraea sputorum and Pandoraea pnomenusa (97.2-99.7 % 16S rRNA gene sequence similarity). The isolates could be differentiated from their closest relatives on the basis of several phenotypic characteristics. The major cellular fatty acid profiles of the isolates comprised C₁₆:₀, C₁₈:₁ω7c, C₁₇:₀ cyclo and summed feature 3 (C₁₆:₁ω7c and/or iso-C₁₅:₀ 2-OH). On the basis of DNA-DNA hybridization studies and phylogenetic analyses, the isolates represent three novel species within the genus Pandoraea, for which the names Pandoraea oxalativorans sp. nov. (TA25(T) = NBRC 106091(T) = CCM 7677(T) = DSM 23570(T)), Pandoraea faecigallinarum sp. nov. (KOx(T) = NBRC 106092(T) = CCM 2766(T) = DSM 23572(T)) and Pandoraea vervacti sp. nov. (NS15(T) = NBRC 106088(T) = CCM 7667(T) = DSM 23571(T)) are proposed.
Topics: Aerobiosis; Bacterial Typing Techniques; Burkholderiaceae; Cluster Analysis; Culture Media; DNA Gyrase; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Molecular Sequence Data; Nucleic Acid Hybridization; Oxalates; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 20952546
DOI: 10.1099/ijs.0.026138-0 -
European Journal of Clinical... Nov 2021A 26-year-old girl with a longstanding colonization by Pandoraea nosoerga underwent liver-lung transplantation for cystic fibrosis (CF) in 2018. Her brother also...
A 26-year-old girl with a longstanding colonization by Pandoraea nosoerga underwent liver-lung transplantation for cystic fibrosis (CF) in 2018. Her brother also suffering from CF was also colonized by P. nosoerga. Despite appropriate perioperative antibiotic therapy, she had post-transplant bacteremic pneumonia caused by extensively drug-resistant P. nosoerga. Drug repurposing was used to optimize treatment options. The cause of post-transplant contamination was studied by comparative whole-genome sequencing including pre- and post-transplant strains and her brother's strains. Post-transplant contamination appeared to be due to her own pre-transplant strain, emphasizing the urgent need to study and implement effective decontamination protocols before transplantation.
Topics: Adult; Anti-Bacterial Agents; Burkholderiaceae; Cystic Fibrosis; Fatal Outcome; Female; Gram-Negative Bacterial Infections; Humans; Liver; Liver Transplantation; Lung; Lung Transplantation; Postoperative Complications
PubMed: 33830365
DOI: 10.1007/s10096-021-04235-0 -
Bioresource Technology Jun 2018Lignin depolymerization is a challenging process in biorefinery due to the recalcitrant and complex structure of lignin. This challenge was herein addressed via...
Lignin depolymerization is a challenging process in biorefinery due to the recalcitrant and complex structure of lignin. This challenge was herein addressed via elaborating a new strategy of combining the bacterial strain Pandoraea sp. B-6 (hereafter B-6) with a deep eutectic solvent (DES) to pretreat rice straw (RS). In this approach, DES effectively depolymerized lignin yet easily caused sugar loss under severe conditions. B-6 not only overcame the obstacle of lignin droplets, but also significantly improved enzymatic digestibility. After B-6 assisted DES pretreatment, the reducing sugar yield increases by 0.3-1.5 times over DES pretreatment and 0.9-3.1 times over the untreated RS. Furthermore, a "cornhusking" mechanism explaining the improvement of the enzymatic digestibility by B-6 was suggested based on physicochemical characterizations of the untreated and pretreated RS. The findings provided a comprehensive perspective to establish a DES-microbial process for lignocellulose pretreatment.
Topics: Carbohydrates; Hydrolysis; Lignin; Oryza; Polymerization; Solvents
PubMed: 29482167
DOI: 10.1016/j.biortech.2018.02.029