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The Medical Journal of Australia Jan 1978
Topics: Humans; Paraquat
PubMed: 651718
DOI: 10.5694/j.1326-5377.1978.tb112488.x -
The Medical Journal of Malaysia Jun 1978
Topics: Adolescent; Adult; Child; Female; Humans; Male; Paraquat
PubMed: 732620
DOI: No ID Found -
Ryoikibetsu Shokogun Shirizu 1994
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Medicina Clinica Jun 1989
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Adverse Drug Reactions and Acute... 1988
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Zhonghua Lao Dong Wei Sheng Zhi Ye Bing... Jul 2015
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Human & Experimental Toxicology 2023We aimed to explore the mechanisms underlying paraquat (PQ)-induced damage using cell lines (NCTC1469, TC-1, TCMK-1) and bioinformatic analysis of the GSE153959 dataset....
OBJECTIVE
We aimed to explore the mechanisms underlying paraquat (PQ)-induced damage using cell lines (NCTC1469, TC-1, TCMK-1) and bioinformatic analysis of the GSE153959 dataset. Assessment of changes in the expression of ferroptosis-related genes in cellular damage due to paraquat poisoning and the important value of these genes in the pathogenesis.
METHODS
Data were retrieved from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) related to ferroptosis were identified by Venn plots and analyzed for enrichment. Proteins encoded by these DEGs were studied for interactions. qRT-PCR and western blotting analyses of cultured cells were used to determine the expression of ferroptosis-related DEGs and their corresponding protein levels.
RESULTS
We identified 25 DEGs primarily involved in epidermal growth factor receptor signaling, apoptotic signaling pathways, endoplasmic reticulum (ER) stress, and ferroptosis. From these, we uncovered eight ferroptosis-related DEGs, four of which were involved in ER response and regulators of ferroptosis- (ChaC glutathione specific gamma-glutamylcyclotransferase 1), (activating transcription factor 3), (transferrin receptor), and (solute carrier family 7 member 11). Significant changes in mRNA and protein levels of CHAC1, ATF3, TFRC, and SLC7A11 were confirmed in PQ-exposed cells.
CONCLUSION
ER stress and ferroptosis are critical for PQ-induced cell damage. CHAC1, ATF3, TFRC, and SLC7A11 are essential molecules implicated in PQ-induced ferroptosis that may serve as therapeutic targets for the amelioration of PQ poisoning.
Topics: Paraquat; Ferroptosis; Apoptosis; Cells, Cultured; Cell Line
PubMed: 36960817
DOI: 10.1177/09603271231167585 -
The International Journal of Artificial... May 1989
Topics: Humans; Paraquat
PubMed: 2737766
DOI: No ID Found -
Biomedical Chromatography : BMC Mar 2012Paraquat (PQ) is an herbicide implicated in numerous fatalities, mainly caused by voluntary ingestion. Several methods have been used to quantify PQ in plasma and urine... (Review)
Review
Paraquat (PQ) is an herbicide implicated in numerous fatalities, mainly caused by voluntary ingestion. Several methods have been used to quantify PQ in plasma and urine samples of intoxicated humans as a predictor of clinical outcome. There is no validated method for the analysis of PQ in postmortem samples. Therefore, the aim of this study was to develop an analytical method, using gas chromatography-ion trap mass spectrometry (GC-IT/MS) after solid-phase extraction, to quantify PQ in postmortem samples, namely in whole blood, urine, liver, lung and kidney, to cover the routes of distribution, accumulation and elimination of PQ. The method proved to be selective as there were no interferences of endogenous compounds with the same retention time as PQ and ethyl paraquat (internal standard). The regression analysis for PQ was linear in the range 0-10 µg/mL. The detection limits ranged from 0.0076 µg/mL for urine to 0.047 µg/mL for whole blood, and the recoveries were suitable for forensic analysis. The proposed GC-IT/MS method provided an accurate and simple assay with adequate precision and recovery for the quantification of PQ in postmortem samples. The proof of applicability was performed in two fatal PQ intoxications. A review of the analytical methods for the determination of quaternary ammonium herbicides is also provided for a better understanding of the presently available techniques.
Topics: Gas Chromatography-Mass Spectrometry; Humans; Limit of Detection; Paraquat; Postmortem Changes
PubMed: 21656535
DOI: 10.1002/bmc.1663 -
Nihon Rinsho. Japanese Journal of... Feb 1995