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The Pediatric Infectious Disease Journal Apr 2004Pasteurella multocida has been reported to cause peritoneal dialysis-associated peritonitis after a cat bite or scratch to the catheter. We report a teenager with... (Review)
Review
Pasteurella multocida has been reported to cause peritoneal dialysis-associated peritonitis after a cat bite or scratch to the catheter. We report a teenager with hamster bite peritonitis caused by P. aerogenes, an organism predominantly isolated from swine.
Topics: Adolescent; Animals; Bites and Stings; Ceftazidime; Cricetinae; Drug Therapy, Combination; Female; Follow-Up Studies; Humans; Injections, Intraperitoneal; Pasteurella; Pasteurella Infections; Peritonitis; Risk Assessment; Treatment Outcome; Vancomycin
PubMed: 15071300
DOI: 10.1097/00006454-200404000-00023 -
Journal of Tropical Pediatrics Feb 2018'Asymptomatic bacteriuria' (ASB) is isolation of a specified quantitative count of bacteria in an appropriately collected urine specimen obtained from a person without...
'Asymptomatic bacteriuria' (ASB) is isolation of a specified quantitative count of bacteria in an appropriately collected urine specimen obtained from a person without symptoms or signs referable to urinary infection. Catheterized specimens are less likely to be contaminated compared with voided specimens; therefore, positive cultures of catheterized specimens are more likely to reflect true bladder bacteriuria even with low colony counts. The common pathogens for ASB are Escherichia coli, Klebsiella and Streptococcus spp. Pasteurella spp. was not previously reported as an ASB agent. ASB is important for pregnant women, children, individuals with obstructive uropathy, chronic renal failure and neutropenia, before the urologic procedures and after renal transplantation. Treatment of ASB is required for above situations. We report an 11-year-old-girl with neurogenic bladder who made clean intermittent catheterization and had Pasteurella aerogenes as an ASB agent.
Topics: Animals; Anti-Bacterial Agents; Bacteriuria; Child; Female; Humans; Pasteurella; Pasteurella Infections; Rabbits; Urinary Bladder, Neurogenic; Urinary Catheterization
PubMed: 28444291
DOI: 10.1093/tropej/fmx028 -
Zentralblatt Fur Veterinarmedizin.... Apr 1976
Topics: Animals; Pasteurella; Swine
PubMed: 1266448
DOI: 10.1111/j.1439-0450.1976.tb00681.x -
Poultry Science Dec 2022Identification and analysis of the antimicrobial resistance of Pasteurella aerogenes (P. aerogenes) isolated from poultry. For susceptibility testing in accordance with...
Identification and analysis of the antimicrobial resistance of Pasteurella aerogenes (P. aerogenes) isolated from poultry. For susceptibility testing in accordance with the CLSI, plasmids were extracted via alkaline lysis and transferred by CaCl treatment. Genomic DNA of a representative P. aerogenes isolate was subjected to whole genome sequencing. CCCP was utilized to determine whether SF190908 contains an efflux pump. The bla gene was ligated with the pET-28 plasmid and transferred to Escherichia coli to verify it as an ESBL gene. SF190908 isolated from poultry was identified as P. aerogenes based upon biochemical and 16s rRNA results. The isolate showed high MIC values for eight antimicrobials. Sequencing results showed that the mobile element-mediated antimicrobial resistance gene cluster conferred antimicrobial resistance on the strain, and a single 5,105-bp plasmid, designated pRCAD0752PA-1, was isolated. Four antimicrobial resistance gene clusters were identified in the SF190908 chromosome; one antimicrobial resistance gene cluster carried the bla gene, which was verified as ESBL according to the CLSI and was detected in Pasteurellaceae for the first time, to the best of our knowledge. The efflux pump may confer antimicrobial resistance to SF190908. P. aerogenes isolated from poultry showed resistance genes encoded in mobile elements that confer multi-antimicrobial resistance to SF190908. The antimicrobial-resistant plasmid pRCAD0752PA-1 was isolated in SF190908 and conferred resistance to florfenicol. This study indicates an urgent need to increase efforts to monitor the spread of P. aerogenes multi-antimicrobial-resistant strains and plasmids, especially in newly discovered at-risk species such as poultry.
Topics: Animals; Pasteurella; Drug Resistance, Bacterial; RNA, Ribosomal, 16S; Chickens; Plasmids; Escherichia coli Infections; Escherichia coli; Anti-Bacterial Agents; beta-Lactamases; Microbial Sensitivity Tests
PubMed: 36274437
DOI: 10.1016/j.psj.2022.102207 -
International Journal of Systematic and... Jan 2005The aim of this study was to improve the definition and identification of a group of veterinarily important bacteria referred to as the [Pasteurella]...
The aim of this study was to improve the definition and identification of a group of veterinarily important bacteria referred to as the [Pasteurella] aerogenes-[Pasteurella] mairii-[Actinobacillus] rossii complex. These organisms have mainly been isolated from the reproductive and intestinal tracts of pigs and in most cases have been considered as opportunistic pathogens. A collection of 87 strains were characterized by phenotypic analysis from which 41 strains were selected for 16S rRNA gene sequence comparison, out of which 23 have been sequenced in the present study. One group of 21 strains phenotyped as biovars 1, 3-5, 9-11, 19 and 25-27, including the type strain of [P.] aerogenes, showed 16S rRNA gene sequence similarities of 99.6 % or higher; another group of 18 strains including biovars 2, 6-8, 12-15, 21, 23, 24 and 26A and the type strain of [A.] rossii showed 97.8 % or higher 16S rRNA gene sequence similarity. Between the two groups, 93.8-95.7 % 16S rRNA gene sequence similarity was observed. Strains of [P.] mairii showed 99.5 % similarity, with 95.5-97.2 and 93.8-95.5 % similarity to strains of [P.] aerogenes and [A.] rossii, respectively. Four strains could not be classified with any of these groups and belonged to other members of Pasteurellaceae. Comparisons were also made to DNA-DNA hybridization data. Biovars 1, 9, 10, 11 and 19, including the type strain of [P.] aerogenes, linked at 70 % DNA reassociation, whereas strains identified as biovars 2, 6, 7, 8, 12, 15 and 21 of [P.] aerogenes linked at 81 %. The latter group most likely represents [A.] rossii based on the 16S rRNA gene sequence comparisons. DNA reassociation between the [P.] aerogenes and [A.] rossii groups was at most 37 %, whereas 47 % was the highest DNA reassociation found between [P.] aerogenes and [P.] mairii. The study showed that [P.] aerogenes, [P.] mairii and [A.] rossii can not be easily separated and may consequently be misidentified based on current knowledge of their phenotypic characteristics. In addition, these taxa are difficult to separate from other taxa of the Pasteurellaceae. A revised scheme for separation based upon phenotypic characters is suggested for the three species [P.] aerogenes emend., [P.] mairii emend. and [A.] rossii emend., with the respective type strains ATCC 27883T, NCTC 10699T and ATCC 27072T.
Topics: Animals; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Genes, rRNA; Genotype; Molecular Sequence Data; Nucleic Acid Hybridization; Pasteurella; Pasteurella Infections; Phenotype; RNA, Ribosomal, 16S; Swine; Swine Diseases
PubMed: 15653877
DOI: 10.1099/ijs.0.63119-0 -
Lancet (London, England) Feb 1994
Topics: Adult; Female; Fetal Death; Fetal Diseases; Fetus; Humans; Pasteurella; Pasteurella Infections; Pregnancy; Vagina
PubMed: 7905986
DOI: 10.1016/s0140-6736(94)92736-7 -
Scandinavian Journal of Infectious... 1996Pasteurella aerogenes is rarely isolated from human specimens. The species is found in the digestive tract of pigs. From 1976 to 1994 7 strains were cultured in Denmark... (Comparative Study)
Comparative Study
Pasteurella aerogenes is rarely isolated from human specimens. The species is found in the digestive tract of pigs. From 1976 to 1994 7 strains were cultured in Denmark from wounds or ulcers. Five patients were bitten by pigs and 2 patients with ulcers were employed in pig farming. A mixture of bacterial species was often found. All 7 strains of P. aerogenes were susceptible to ampicillin, cephalosporins and ciprofloxacin. Ability to hydrolyse urea, to produce oxidase and catalase, to decarboxylate ornithine and to produce gas from glucose and inability to produce indole was characteristic for P. aerogenes. Most bite wounds were located on the lower lateral part of the thigh. Foul smelling pus and abscess formation was the rule. Incision, drainage and antibiotic treatment were usually necessary.
Topics: Adult; Aged; Agricultural Workers' Diseases; Animals; Anti-Bacterial Agents; Bites and Stings; Denmark; Drainage; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Occupational Exposure; Pasteurella; Pasteurella Infections; Swine; Ulcer; Wound Infection
PubMed: 9060058
DOI: 10.3109/00365549609037962 -
Antimicrobial Agents and Chemotherapy Oct 2001Tetracycline-resistant Pasteurella aerogenes isolates obtained from the intestinal tract of swine were investigated for their tet genes by PCR analysis and hybridization...
Tetracycline-resistant Pasteurella aerogenes isolates obtained from the intestinal tract of swine were investigated for their tet genes by PCR analysis and hybridization experiments. In contrast to Pasteurella isolates from the respiratory tract, tet(H) genes were detected in the chromosomal DNA of only 2 of the 24 isolates, one of which also carried two copies of a tet(B) gene. All other P. aerogenes isolates carried tet(B) genes, which are the predominant tet genes among Enterobacteriaceae. A single isolate harbored a tet(B) gene as part of a truncated Tn10 element on the 4.8-kb plasmid pPAT2. Comparative analysis of the pPAT2 sequence suggested that the Tn10 relic on plasmid pPAT2 is the result of several illegitimate recombination events. The remaining 21 P. aerogenes isolates carried one or two copies of the tet(B) gene in their chromosomal DNA. In the majority of the cases, these tet(B) genes were associated with copies of Tn10 as confirmed by their SfuI and BamHI hybridization patterns. No correlation between the number of tet gene copies and the MICs of tetracycline, doxycyline and minocycline was observed.
Topics: Animals; Bacterial Proteins; Chromosomes, Bacterial; Genotype; Intestines; Molecular Sequence Data; Pasteurella; Plasmids; Swine; Tetracycline Resistance
PubMed: 11557485
DOI: 10.1128/AAC.45.10.2885-2890.2001 -
FEMS Microbiology Letters Jul 2000Molecular analysis of Pasteurella isolates of animal origin for plasmid-encoded tetracycline resistance genes identified a common tet(H)-carrying plasmid of 5.5 kbp in a... (Comparative Study)
Comparative Study
Molecular analysis of Pasteurella isolates of animal origin for plasmid-encoded tetracycline resistance genes identified a common tet(H)-carrying plasmid of 5.5 kbp in a single isolate of Pasteurella aerogenes and six isolates of Pasteurella multocida. This plasmid carried a truncated Tn5706 element in which one of the IS elements, IS1596, was lost completely and of the other, IS1597, only a relic of 84 bp was left. Sequencing of the resistance gene region and the flanking areas revealed the presence of a deletion in the 3' end of the tet(H) gene which shortened the tet(H) reading frame by 24 bp. The amino acid sequence of the respective TetH protein comprised only 392 amino acids. Despite this deletion, the tet(H) gene conferred high level tetracycline resistance not only to the original Pasteurella isolates but also to the respective Escherichia coli JM107 and C600 transformants as confirmed by MIC determination. The deletion was probably the result from recombinational events. Two possible recombination sites involved in the deletion of tet(H) and that of IS1597 were identified. Macrorestriction analysis of the Pasteurella isolates carrying plasmid pPAT1 confirmed horizontal and vertical transfer of this plasmid.
Topics: Animals; Anti-Bacterial Agents; Cattle; DNA Transposable Elements; Electrophoresis, Gel, Pulsed-Field; Escherichia coli; Gene Deletion; Genes, Bacterial; Microbial Sensitivity Tests; Pasteurella; Plasmids; Recombinant Proteins; Repressor Proteins; Sequence Homology, Amino Acid; Swine; Tetracycline; Tetracycline Resistance; Transformation, Bacterial
PubMed: 10913704
DOI: 10.1111/j.1574-6968.2000.tb09192.x -
Zentralblatt Fur Bakteriologie :... Jun 1993On the occasion of five Danish human Pasteurella aerogenes from pig bite lesions, a comparison was made between 6 isolates from man and 15 animal isolates, mainly from...
On the occasion of five Danish human Pasteurella aerogenes from pig bite lesions, a comparison was made between 6 isolates from man and 15 animal isolates, mainly from pigs. The strains originated from 6 different countries (USA, Canada, Czechoslovakia, France, Belgium and Denmark). The 21 isolates were characterized by conventional biochemical tests, antibiogram and the API 20 NE kit; finally ribotyping was carried out by hybridizing EcoRI-digested chromosomal DNA with a probe derived from E. coli ribosomal RNA. By ribotyping, 19 of the 21 strains clustered at a similarity level of 81% or more; both phenotypical tests and ribotyping indicated that the remaining two strains did not belong to the species P. aerogenes. In conclusion, despite minor differences our P. aerogenes isolates constituted a well-defined group and they could not be subdivided on basis of animal or geographical origin.
Topics: Animals; Anti-Bacterial Agents; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Deoxyribonuclease EcoRI; Humans; Pasteurella; Phenotype; Polymorphism, Restriction Fragment Length; RNA, Bacterial; RNA, Ribosomal; Swine
PubMed: 7690274
DOI: 10.1016/s0934-8840(11)80493-7