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Carbohydrate Polymers Sep 2022Roots of Aconitum carmichaelii are used in Asian countries due to its content of bioactive alkaloids. In the production of root preparations, tons of leaves are usually...
Roots of Aconitum carmichaelii are used in Asian countries due to its content of bioactive alkaloids. In the production of root preparations, tons of leaves are usually discarded, leading to a huge waste of herbal material. The aim of this study is to investigate the polysaccharides in these unutilized leaves. A neutral polysaccharide (AL-N) appeared to be a mixture of heteromannans, and two purified acidic polysaccharides (AL-I-I and AL-I-II) were shown to be pectins containing a homogalacturonan backbone substituted with terminal β-Xylp-units. AL-I-I consisted of a type-I rhamnogalacturonan core, with arabinan and type-II arabinogalactan domains while AL-I-II was less branched. AL-N and AL-I-I were able to modulate the complement system, while AL-I-II was inactive. Interestingly, AL-N, AL-I-I and AL-I-II were shown to exert anti-inflammatory effects on porcine enterocyte IPEC-J2 cells. AL-I-I and AL-I-II were able to down-regulate the expression of toll-like receptor 4 (TLR4) and nucleotide-binding oligomerization domain 1 (NOD1).
Topics: Aconitum; Alkaloids; Animals; Anti-Inflammatory Agents; Cell Line; Enterocytes; Plant Leaves; Polysaccharides; Swine
PubMed: 35698356
DOI: 10.1016/j.carbpol.2022.119655 -
Environmental Science and Pollution... May 2021There are some key processes that regulate cadmium (Cd) accumulation in rice. Understanding the characteristics and mechanisms of Cd accumulation in high Cd-accumulating...
There are some key processes that regulate cadmium (Cd) accumulation in rice. Understanding the characteristics and mechanisms of Cd accumulation in high Cd-accumulating rice lines benefits for excavating relevant genes. Cd accumulation and distribution in roots of Lu527-8, a high Cd-accumulating rice line, were investigated by a hydroponic experiment, with a control of a normal rice line (Lu527-4). Lu527-8 showed significantly higher Cd concentrations in roots than Lu527-4. More than 81% of Cd in roots of two rice lines is distributed in soluble fraction and cell wall. In soluble fraction, there were more organic acids, amino acids, and phytochelatins in Lu527-8, benefiting Cd accumulation. Pectin and hemicellulose 1 (HC1), especially pectin, were main polysaccharides in cell wall. Lu527-8 showed more pectin and HC1 along with higher pectin methylesterase (PME) activity compared with Lu527-4, promoting Cd accumulation. Besides, Lu527-8 showed higher Cd translocation from root to shoot due to more amounts of ethanol-extractable Cd in roots than Lu527-4. In conclusion, specific characteristics of Cd chemical forms and subcellular distribution in roots of high Cd-accumulating rice line are important for Cd accumulation and translocation.
Topics: Cadmium; Hydroponics; Oryza; Plant Roots; Soil Pollutants
PubMed: 33462687
DOI: 10.1007/s11356-021-12373-3 -
Journal of Pediatric Surgery Feb 2002Intestinal neuronal dysplasia (IND) is a complex alteration of the enteric nervous system (ENS) that may involve rectum, colon, or the whole intestine. This disorder is...
BACKGROUND/PURPOSE
Intestinal neuronal dysplasia (IND) is a complex alteration of the enteric nervous system (ENS) that may involve rectum, colon, or the whole intestine. This disorder is a frequent cause of intestinal dysmotility and pseudo-obstruction in the first 3 years of life. The aim of this study was to identify possible associations and correlations of IND with other gastrointestinal and nongastrointestinal anomalies.
METHODS
From 1986 to 2000, 95 cases of IND type B without aganglionosis were diagnosed. Fifteen cases were diffuse IND, whereas the remaining 80 were rectocolonic neuronal dysplasia. The diagnosis was performed on rectal suction biopsy specimens taken 2 to 10 cm above the pectinate line. Acetylcholinesterase (AChE), lactic dehydrogenase (LDH), and NADPH-diaphorase (NADPH-d) histochemical techniques were performed on serial cryostatic sections. We used Schärli and Meier-Ruge criteria (1981) for the diagnosis of IND until 1992, when we adopted Borchard et al criteria (1991). A retrospective analysis of the clinical data was performed to identify IND-associated anomalies.
RESULTS
These anomalies included anorectal malformations (9 cases), intestinal malrotation (8), megacystis (5), congenital short small bowel (4), hypertrophic pyloric stenosis (3), necrotizing enterocolitis (2), mental retardation (2), short stature (2), facial dysmorphism (2), Down syndrome (1), intestinal atresia (1), diffuse intestinal angiomatosis (1), histiocytosis (1), microvillus agenesia (1), and hearing loss (1). Overall, 43 associated anomalies were found in 29 IND cases (30.5%). Gastrointestinal anomalies accounted for 67.4% (29 of 43 anomalies) of associated disorders. The incidence of associated anomalies was higher in diffuse IND (80% of cases, 12 of 15) than in rectocolonic forms (21.2%, 17 of 80).
CONCLUSIONS
Unlike Hirschsprung's disease, which is determined genetically, IND pathogenesis is unknown. The analysis of associated anomalies in IND population is an important clinical approach to investigate possible pathogenetic correlations. Two recessive syndromes were identified (3 families). The first was characterized by IND, intestinal malrotation, and congenital short bowel, the second by IND, short stature, mental retardation, and facial dysmorphism. In this study, gastrointestinal anomalies accounted for 67.4% of all associated disorders. These data suggest a strong correlation between IND and intestinal development. Abnormalities of the fetal ENS could determine the IND phenotype, which is likely to contribute to the pathogenesis of different intestinal malformations and in particular of anorectal and "rotation" anomalies.
Topics: Abnormalities, Multiple; Adolescent; Biopsy, Needle; Child; Child, Preschool; Comorbidity; Digestive System Abnormalities; Enteric Nervous System; Female; Humans; Incidence; Infant; Infant, Newborn; Intestines; Male; Pedigree; Retrospective Studies; Short Bowel Syndrome
PubMed: 11819202
DOI: 10.1053/jpsu.2002.30258 -
Bio-medical Materials and Engineering 2018Novel pectin-honey hydrogels have been developed and characterized as medical device. Ideally, a wound dressing should maintain optimal fluid affinity, permit moisture...
BACKGROUND
Novel pectin-honey hydrogels have been developed and characterized as medical device. Ideally, a wound dressing should maintain optimal fluid affinity, permit moisture evaporation, protect the wound from microbes, and have shape-conformability, biocompatibility, and antibacterial activity.
OBJECTIVE
A novel, simple and fast method to produce pectin-honey wound dressings is described.
METHODS
The properties of these pectin-honey hydrogels were investigated, including swelling ability, water vapour transmission rate, hydrogen peroxide production, methylglyoxal content and antibacterial activity. Biocompatibility was assessed by proliferation assays using cultured fibroblast cells and by in vivo study with subcutaneous and intraperitoneal implantation in rats.
RESULTS
Hydrogel showed a good water vapour transmission rate, fluid uptake and were not cytotoxic for fibroblasts. The hydrogel demonstrated good antibacterial activity toward clinically relevant pathogens, including S. aureus and E. coli. Biocompatibility was confirmed by the measurement of plasma levels of interleukin (IL)1 beta, IL-6, tumour necrosis factor (TNF) alpha, and prostaglandin (PG)E2. No histological changes were observed.
CONCLUSIONS
The presence of a natural active component, conformability, and complete resorbability are the main characteristics of this new biocompatible biomaterial that is well tolerated by the body, possibly improves healing, may be used for surgical complications prevention, with a simple and inexpensive production process.
Topics: Animals; Anti-Bacterial Agents; Bandages; Biocompatible Materials; Cell Line; Escherichia coli; Escherichia coli Infections; Fibroblasts; Honey; Hydrogels; Male; Materials Testing; Mice; Pectins; Rats, Sprague-Dawley; Staphylococcal Infections; Staphylococcus aureus; Wound Healing
PubMed: 29578463
DOI: 10.3233/BME-181730 -
Environmental Research Aug 2023The current study aimed to investigate the toxicity of positively charged polyhexamethylene guanidine (PHMG) polymer and its complexation with different anionic natural...
The current study aimed to investigate the toxicity of positively charged polyhexamethylene guanidine (PHMG) polymer and its complexation with different anionic natural polymers such as k-carrageenan (kCG), chondroitin sulfate (CS), sodium alginate (Alg.Na), polystyrene sulfonate sodium (PSS.Na) and hydrolyzed pectin (HP). The physicochemical properties of the synthesized PHMG and its combination with anionic polyelectrolyte complexes (PECs) namely PHMG:PECs were characterized using zeta potential, XPS, FTIR, and TG analysis. Furthermore, cytotoxic behavior of the PHMG and PHMG:PECs, respectively, were evaluated using human liver cancer cell line (HepG2). The study results revealed that the PHMG alone had slightly higher cytotoxicity to the HepG2 cells than the prepared polyelectrolyte complexes such as PHMG:PECs. The PHMG:PECs showed a significant reduction of cytotoxicity to the HepG2 cells than the pristine PHMG alone. A reduction of PHMG toxicity was observed may be due to the facile formation of complexation between the positively charged PHMG and negatively charged anionic natural polymers such as kCG, CS, Alg. Na, PSS.Na and HP, respectively, via charge balance or neutralization. The experimental results indicate that the suggested method might significantly lower PHMG toxicity while improving biocompatibility.
Topics: Humans; Disinfectants; Guanidine; Polyelectrolytes; Guanidines; Cell Line
PubMed: 37201704
DOI: 10.1016/j.envres.2023.116172 -
International Journal of Biological... Apr 2021Platycodonis Radix is widely used as homology of medicine and food in China; polysaccharides are thought to be one of its functional constituents. In this study, a...
Platycodonis Radix is widely used as homology of medicine and food in China; polysaccharides are thought to be one of its functional constituents. In this study, a pectic polysaccharide, PGP-I-I, was obtained from the root of the traditional medicine plant Platycodon grandiflorus through ion exchange chromatography and gel filtration. This was characterized being mainly composed of 1,5-α-L-arabinan and both arabinogalactan type I (AG-I) and II chains linked to rhamnogalacturonan I (RG-I) backbone linked to longer galacturonan chains. In vitro bioactivity study showed that PGP-I-I could restore the intestinal cellular antioxidant defense under the condition of hydrogen peroxide (HO) treatment through promoting the expressions of cellular antioxidant genes and protect against oxidative damages.
Topics: Animals; Antioxidants; Cell Line; Chromatography, Gel; Chromatography, Ion Exchange; Dietary Carbohydrates; Galactans; Hydrogen Peroxide; Pectins; Plant Extracts; Plant Roots; Platycodon; Polysaccharides; Swine
PubMed: 33571586
DOI: 10.1016/j.ijbiomac.2021.02.041 -
Membranes Aug 2022Composite bacterial cellulose (BC) based hydrogel with alginate (A) or pectin (P) or alginate and pectin was fabricated via a physical crosslinking technique using...
Composite bacterial cellulose (BC) based hydrogel with alginate (A) or pectin (P) or alginate and pectin was fabricated via a physical crosslinking technique using calcium chloride (CaCl) solution and incorporated with polyhexamethylene biguanide (PHMB) as an effective antimicrobial drug by immersion method. After that, the physicochemical properties of all hydrogel formulations were characterized. The result showed that the formulations with PHMB performed better physicochemical properties than the hydrogel without PHMB. Fourier transform infrared spectroscopy (FT-IR) showed the interaction between PHMB and the carboxylic group of alginate and pectin. BC/A-PHMB hydrogel performed suitable mechanical strength, fluid uptake ability, water retention property, drug content, high integrity value, and maximum swelling degree. Moreover, in vitro cell viability of BC/A-PHMB hydrogel revealed high biocompatibility with human keratinocyte cell line (HaCaT) and demonstrated prolong released of PHMB in Tris-HCl buffer pH 7.4, while rapid release in phosphate buffer saline pH 7.4. BC/A-PHMB hydrogel demonstrated good anti-bacterial activity against and . In conclusion, BC/A-PHMB hydrogel could be a potential dual crosslinked ion-based hydrogel for wound dressing with anti-bacterial activity.
PubMed: 36135844
DOI: 10.3390/membranes12090825 -
Carbohydrate Polymers Mar 2017Pectic Polysaccharide (PP) from dietary sources has been known to prevent cancer growth and hence impede cancer progression. We evaluated anticancer effect of...
Pectic Polysaccharide (PP) from dietary sources has been known to prevent cancer growth and hence impede cancer progression. We evaluated anticancer effect of Pectic-Oligosaccharide isolated from Sour Raw Tomato (SrTPO); its bioavailability and structure elucidated from purified fraction (SrTPO1). SrTPO1 inhibited galectin-3 activity with MIC of 0.25 ug/mL (100 fold better than standard galactose), inhibited the growth of AGS cells (IC 3.4μg/mL) and induced apoptosis (70% inhibition at 30μg/mL concentration). Normal- NIH 3T3 cells were not affected by SrTPO as opposed to doxorubicin, a known anticancer drug, which reduced 76% viability at equivalent dose. SrTPO1 was identified as RhamnogalacturonanI-arabinogalactan (RGI-AG), where repeated alternative rhamnose and galacturonic acid residues were observed while arabinose in the branch point and β-1,4 linked galactose in the linear chain form. SrTPO was found to be bioavailable as evaluated by FITC labelled oligos inside the cell, which was in reciprocal proportion with apoptosis.
Topics: Antineoplastic Agents, Phytogenic; Apoptosis; Cell Line, Tumor; Humans; Solanum lycopersicum; Oligosaccharides; Pectins; Stomach Neoplasms; Structure-Activity Relationship
PubMed: 28115100
DOI: 10.1016/j.carbpol.2016.12.046 -
Genes Jun 2017Methylation modifications play an important role in multiple biological processes. Several studies have reported altered methylation patterns in male sterile plants such...
Methylation modifications play an important role in multiple biological processes. Several studies have reported altered methylation patterns in male sterile plants such as rice and wheat, but little is known about the global methylation profiles and their possible roles in the cabbage () male sterile line. In this study, single-base-resolution bisulfite sequencing (BS-Seq) was adopted to identify the pattern and degree of cytosine methylation in the male sterile line 01-20S and its near-isogenic fertile line 01-20F. Similar methylation patterns were profiled, with some changes observed in local positions. In total, 505 differentially methylated genomic regions (DMRs) and 106 DMR-associated genes were detected. Nine genes related to pollen development were discovered and further validated by a quantitative reverse-transcription polymerase chain reaction (qRT-PCR). Among these, four were downregulated in 01-20S. In particular, (an invertase/pectin methylesterase inhibitor family protein) is likely involved in pectin degradation, and might play an important role in the pollen separation defects of 01-20S. This study facilitates a better understanding of DNA methylation alterations and their possible roles in genic male sterility in cabbages.
PubMed: 28621722
DOI: 10.3390/genes8060159 -
Ecotoxicology and Environmental Safety Dec 2021Exploring the mechanism of cadmium (Cd) accumulation in Cd-safe rice lines is beneficial for ensuring rice safety. D62B, a Cd-safe rice line, accumulates less than...
Exploring the mechanism of cadmium (Cd) accumulation in Cd-safe rice lines is beneficial for ensuring rice safety. D62B, a Cd-safe rice line, accumulates less than 0.2 mg Cd kg in the brown rice due to strong capacity of Cd retention in the roots, and the root cell wall (RCW) polysaccharides play important roles. However, specific underlying mechanism of Cd binding on the polysaccharides is little known. In this study, the role of polysaccharides, especially pectin and hemicellulose 1 (HC1), in RCW of D62B was investigated by adsorption experiments and Fourier Transform Infrared Spectroscopy (FTIR) analysis compared with a common rice line (Luhui17). Cadmium was adsorbed on RCW of two rice lines by a multilayer and inhomogeneous chemisorption way with the force of ion transfer or exchange. Cadmium was adsorbed on RCW rapidly at first stage with the limit of internal and external diffusion, and gradually reached saturation. With the removal of pectin, the Cd adsorption rate, maximum Cd adsorption amount and the shift degree of carboxyl groups in the RCW of D62B sharply decreased, which showed advantages compared with Luhui17. Sequential removal of HC1 further decreased the maximum Cd adsorption amount and the shift degree of hydroxyl groups. The results showed that more available functional groups, especially carboxyl groups in pectin and hydroxyl groups in HC1, contributed to Cd immobilization within the RCW of Cd-safe rice line, thus limiting Cd translocation to the shoot and reducing Cd accumulation in the brown rice.
Topics: Cadmium; Cell Wall; Oryza; Plant Roots; Polysaccharides; Soil Pollutants
PubMed: 34592527
DOI: 10.1016/j.ecoenv.2021.112818