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Polish Journal of Microbiology 2014178 yeasts isolated from various foods were identified using ID32 C strip; Saccharomyces cerevisiae (47), Rhodotorula mucilaginosa (19), Candida kefyr (33), Candida...
178 yeasts isolated from various foods were identified using ID32 C strip; Saccharomyces cerevisiae (47), Rhodotorula mucilaginosa (19), Candida kefyr (33), Candida krusei (37), Candida lusitaniae (28), Cndida lipolytica (14). The yeasts were harvested, saponified, derivatized, and extracted, and fatty acid analysis was performed by Gas Chromatography Mass Spectrometry. The yeast lipids contained Decanoic acid (C10:0), Luaric acid (C12:0), Myristoleic acid (C14:1), Myristic acid (C14:0), Pentadecanoic acid (C15:0), Palmitoleic acid C16:1), Pamitic acid (C16:0), Heptadecenoic acid (C17:1), Linoleic acid (C18:2), Linolenic acid (C18:3), Oleic acid (C18:1) and Stearic acid (C 18:0). The differences in the composition of these fatty acids could be used to identify the species as an accurate, efficient and relatively rapid method.
Topics: Fatty Acids; Food Microbiology; Phylogeny; Yeasts
PubMed: 25804068
DOI: No ID Found -
Nuclear Medicine Communications Nov 2019The use of cadmium-zinc-telluride-based scanners may increase the clinical feasibility of simultaneous dual-isotope imaging. In the current study, we sought to...
Shortened acquisition time in simultaneous 99mTc-tetrofosmin and 123I-β-methyl-p-iodophenyl pentadecanoic acid dual-tracer imaging with cadmium-zinc-telluride detectors in patients undergoing primary coronary intervention for acute myocardial infarction.
OBJECTIVE
The use of cadmium-zinc-telluride-based scanners may increase the clinical feasibility of simultaneous dual-isotope imaging. In the current study, we sought to investigate a potential acquisition time in simultaneous Tc-tetrofosmin/I-β-methyl-p-iodophenyl pentadecanoic acid dual-isotope imaging using a Discovery NM/CT 670 cadmium-zinc-telluride.
METHODS
Simultaneous Tc-tetrofosmin/I-β-methyl-p-iodophenyl pentadecanoic acid dual-isotope imaging was performed in 29 patients who had undergone primary percutaneous coronary intervention for acute myocardial infarction. Referenced images with an acquisition time of 65 s/view (16.25 min) were reframed to produce images with acquisition times of 33, 16, and 8 s/view. The values for the quantitative-gated single-photon emission computed tomography (SPECT) and the quantitative perfusion SPECT were compared.
RESULTS
The quantitative-gated SPECT values for images with 33, 16, and 8 s/views showed good consistency with those for 65 s/view (the lower 95% confidence intervals for the intraclass correlation were ≥0.80). The quantitative perfusion SPECT values for Tc-tetrofosmin images with 33, 16, and 8 s/views also showed good consistency with those for 65 s/view; however, the quantitative perfusion SPECT values for I-β-methyl-p-iodophenyl pentadecanoic acid images with an acquisition time of 8 s/view were not consistent with the reference acquisition time of 65 s/view.
CONCLUSIONS
The quantitative-gated SPECT and quantitative perfusion SPECT values obtained from images with shorter acquisition times correlated with the values obtained from images with a reference acquisition time of 65 s/view; however, tracer-specific predisposition should be considered. These findings suggest that it is possible to reduce acquisition time when performing simultaneous Tc-tetrofosmin/I-β-methyl-p-iodophenyl pentadecanoic acid dual-tracer imaging with the novel cadmium-zinc-telluride scanner.
Topics: Acute Disease; Cadmium; Fatty Acids; Female; Humans; Image Processing, Computer-Assisted; Iodobenzenes; Male; Middle Aged; Myocardial Infarction; Organophosphorus Compounds; Organotechnetium Compounds; Percutaneous Coronary Intervention; Tellurium; Time Factors; Tomography, Emission-Computed, Single-Photon; Zinc
PubMed: 31524763
DOI: 10.1097/MNM.0000000000001084 -
The Biochemical Journal Dec 1954
Topics: Fats; Fatty Acids; Food; Humans; Hydrogenation
PubMed: 13229996
DOI: 10.1042/bj0580516 -
International Journal of Clinical... Apr 2013A Malabsorption Blood Test (MBT) is proposed as an alternative method to the 72-hour stool and dietary collection for assessing the degree of fat malabsorption in people...
Diagnosing malabsorption with systemic lipid profiling: pharmacokinetics of pentadecanoic acid and triheptadecanoic acid following oral administration in healthy subjects and subjects with cystic fibrosis.
OBJECTIVE
A Malabsorption Blood Test (MBT) is proposed as an alternative method to the 72-hour stool and dietary collection for assessing the degree of fat malabsorption in people with pancreatic insufficiency. The MBT consists of a simultaneous oral dose of pentadecanoic acid (PA), a free fatty acid, and triheptadecanoic acid (THA), a triglyceride with three heptadecanoic (HA) saturated fatty acids requiring hydrolysis by pancreatic lipase before HA can be intestinally absorbed. The aim of this study is to demonstrate the ability of MBT to detect fat malabsorption in healthy adult subjects using the pancreatic lipase (PL) inhibitor Orlistat (Xenical®), and in subjects with CF and PI while on and off routine pancreatic enzyme doses.
MATERIALS AND METHODS
The MBT with the PA and THA were delivered in a breakfast test meal (2.5 g PA and either 5 g or 8 g THA) to healthy adult subjects (ages 18 - 50 years, BMI 21 - 30) and to subjects with CF (> 12 years, FEV1% predicted > 40%), after a 12-hour fast and 24 hours without dairy foods. Serum levels of PA and HA were assessed by gas-liquid chromatography, from blood samples drawn prior to MBT and then hourly for 8 hours. For healthy subjects, the MBT was administered before and after Orlistat treatment, and in subjects with CF, both with subjects receiving routine pancreatic lipase treatment ("on enzyme") and also "off enzyme" treatment. Treatment groups were compared for baseline (C0) and maximum (Cmax) plasma concentrations of PA and HA over 8 hours: area under the curve (AUC) was calculated using linear trapezoid method. The ratio of HA to PA Cmax and AUC was also calculated and compared.
RESULTS
For the healthy subjects (n = 15, 60% female, ages 21 - 49 years), absorption of HA was reduced 71% for Cmax (p < 0.001) and 65% for AUC (p = 0.001) after Orlistat treatment, and absorption of PA was unchanged. For subjects with CF (n = 6, 50% female, ages 13 - 19 years), absorption of HA was minimal with subjects "off enzymes" and increased significantly with subjects "on enzymes" while absorption of PA did not differ between groups. Enzyme administration resulted in increased Cmax HA/ PA ratios from 0.02 to 0.92 and from 0.05 to 0.73 in subjects with CF receiving 5.0 g and 8.0 g of THA, respectively. AUC HA/PA ratios showed similar increases.
CONCLUSIONS
In this pilot and feasibility proof-of-concept study, the MBT, utilizing the relative absorption of HA to PA, two odd-chained fatty acids, responds to changes in fat absorption in healthy subjects using a lipase inhibitor and in subjects with CF while on or off enzyme therapy. The MBT holds promise to provide a more accurate, specific and acceptable alternative to the 72-hour stool collection to quantify pancreatic-based fat malabsorption in a variety of clinical and research contexts.
Topics: Administration, Oral; Adolescent; Adult; Area Under Curve; Chromatography, Gas; Cystic Fibrosis; Dietary Fats; Fatty Acids; Feasibility Studies; Female; Humans; Lactones; Malabsorption Syndromes; Male; Middle Aged; Orlistat; Pilot Projects; Young Adult
PubMed: 23357842
DOI: 10.5414/CP201793 -
Chemistry Central Journal Jan 2013Fatty acid profiling has been widely used in the bacteria species identification, we hypothesized that fatty acid characteristics might discriminate the Panax herbs...
BACKGROUND
Fatty acid profiling has been widely used in the bacteria species identification, we hypothesized that fatty acid characteristics might discriminate the Panax herbs according to species. To test the hypothesis, fatty acids of Panax species, including Panax ginseng, Panax notoginseng and Panax quinquefolius, were characterized and compared using gas chromatography-mass spectrometry (GC-MS) followed by multivariate statistical analysis.
RESULTS
The content of investigated 11 fatty acids, including myristic acid, pentadecanoic acid, palmitic acid, palmitoleic acid, heptadecanoic acid, stearic acid, oleic acid, linoleic acid, α-linolenic acid, arachidic acid and eicosadienoic acid, obviously varied among three species, suggesting each species has its own fatty acid pattern. Principal component analysis and hierarchical clustering analysis according to the absolute and relative contents of fatty acids, showed that 30 tested samples could be clearly differentiated according to the species.
CONCLUSIONS
These findings demonstrated that GC-MS-based fatty acid profiling coupled with multivariate statistical analysis provides reliable platform to classify these three Panax species, which is helpful for ensuring their safety and efficacy.
PubMed: 23336351
DOI: 10.1186/1752-153X-7-12 -
Current Cardiology Reports Apr 2007The myocardium preferentially oxidizes free fatty acids for energy production. However, the dependency of this metabolic pathway on oxygen makes this process vulnerable... (Review)
Review
The myocardium preferentially oxidizes free fatty acids for energy production. However, the dependency of this metabolic pathway on oxygen makes this process vulnerable to ischemia. The energy requirements of the myocardium are subsequently met by the oxidation of carbohydrates, particularly glucose. Recovery of fatty acid metabolism lags behind restoration of perfusion, resulting in the phenomenon of metabolic stunning. This decrease of fatty acid utilization following ischemia can be imaged with fatty acid radiotracers, particularly beta-Methyl-p-(123)I-iodophenyl pentadecanoic acid (BMIPP), which demonstrates markedly limited metabolism via beta-oxidation, resulting in prolonged retention in the cardiomyocyte. Thus, in patients presenting with chest pain and no prior myocardial infarction, abnormal BMIPP uptake at rest reflects metabolic alteration caused by the preceding ischemia, also termed ischemic memory.
Topics: Animals; Coronary Circulation; Diagnostic Imaging; Fatty Acids; Humans; Metabolic Networks and Pathways; Myocardial Ischemia; Radiopharmaceuticals
PubMed: 17430684
DOI: 10.1007/BF02938343 -
Circulation Journal : Official Journal... Sep 2020
I-β-Methyl Iodophenyl Pentadecanoic Acid and Thallium Dual Myocardial Single-Photon Emission Computed Tomography (BMIPP/Tl SPECT) Detection of Myocardial Damage of Systemic Sclerosis.
Topics: Aged; Fatty Acids; Female; Humans; Iodine Radioisotopes; Iodobenzenes; Japan; Middle Aged; Myocardial Perfusion Imaging; Nicorandil; Scleroderma, Systemic; Thallium Radioisotopes; Tomography, Emission-Computed, Single-Photon; Treatment Outcome; Vasodilator Agents; Ventricular Dysfunction, Left
PubMed: 32848121
DOI: 10.1253/circj.CJ-20-0516 -
Pharmacological Research Jun 2024Accumulating evidence has proved the close association between alterations in gut microbiota and resistance to chemotherapeutic drugs. However, the potential roles of...
Accumulating evidence has proved the close association between alterations in gut microbiota and resistance to chemotherapeutic drugs. However, the potential roles of gut microbiota in regulating oxaliplatin sensitivity in gastric cancer (GC) have not been investigated before. We first found that antibiotic treatment diminished the therapeutic efficacy of oxaliplatin in a GC mouse model. Importantly, this effect could be transmitted to germ-free mice via fecal microbiota transplantation, indicating a potential role of gut microbiota modulation in oxaliplatin efficacy. Further, metagenomics data showed that Akkermansia muciniphila (A. muciniphila) ranked first among the bacterial species with decreased relative abundances after antibiotic treatment. Metabolically active A. muciniphila promotes oxaliplatin efficacy. As shown by metabolomics analysis, the metabolic pattern of gut microbiota was disrupted with significantly downregulated levels of pentadecanoic acid (PEA), and the use of PEA significantly promoted oxaliplatin efficacy. Mechanistically, FUBP1 positively regulated aerobic glycolysis of GC cells to hinder the therapeutic efficacy of oxaliplatin. A. muciniphila-derived PEA functioned as an inhibitory factor of glycolysis by directly antagonizing the activity of FUBP1, which potentiated GC responses to oxaliplatin. Our research suggested a key role for intestinal A. muciniphila and its metabolite PEA in promoting oxaliplatin efficacy, thus providing a new perspective for probiotic and prebiotic intervention in GC patients during chemotherapy.
PubMed: 38908613
DOI: 10.1016/j.phrs.2024.107278 -
European Journal of Nuclear Medicine 1985The uptake and turnover of omega(p 123I iodophenyl-)pentadecanoic acid (I-PPA), a radioiodinated free-fatty-acid analog, was examined in the heart, lung, liver, kidneys,...
The uptake and turnover of omega(p 123I iodophenyl-)pentadecanoic acid (I-PPA), a radioiodinated free-fatty-acid analog, was examined in the heart, lung, liver, kidneys, spleen, and skeletal muscle of rats. At 2 min post injection, a high cardiac uptake of 4.4% dose per gram had already been achieved; this was followed by a rapid, two-component, tracer clearance. The kinetics of tissue concentrations of labeled hydrophilic catabolites indicated a rapid oxidation of I-PPA and the subsequent washout of I-PPA catabolites from heart-muscle tissue. The fractional distribution of the labeled cardiac lipids compared favorably with previously reported values for 3H-oleic- or 14C-palmitic-acid-labeled myocardial lipids. Typical patterns of I-PPA metabolism were observed in tissues depending on primary fatty-acid oxidation, lipid metabolism regulation, or I-PPA-catabolite excretion. The tissue concentrations and kinetics of I-PPA and its metabolites in the heart muscle indicated that general pathways of cardiac-lipid metabolism are traced by this new gamma-emitting isotope-labeled radiopharmaceutical.
Topics: Animals; Iodine Radioisotopes; Iodobenzenes; Kinetics; Male; Myocardium; Rats; Rats, Inbred Strains; Tissue Distribution
PubMed: 3987699
DOI: 10.1007/BF00254466 -
Polymorphism and solid-state miscibility in the pentadecanoic acid-heptadecanoic acid binary system.Chemistry and Physics of Lipids Jul 2008The pentadecanoic acid-heptadecanoic acid (C(15)H(29)OOH-C(17)H(33)OOH) binary system is dealt with in this article. Combined thermal analysis and X-ray powder...
The pentadecanoic acid-heptadecanoic acid (C(15)H(29)OOH-C(17)H(33)OOH) binary system is dealt with in this article. Combined thermal analysis and X-ray powder diffraction experiments are performed to characterize the polymorphism of the pure compounds and of their mixed samples. In particular, modern methods of crystal structure resolution from powder data (direct space methods) are applied in order to investigate and compare the molecular arrangement within the solid phases of the fatty acids considered. A proposal of the binary phase diagram is given. It exhibits no less than eight distinct solid phases stabilized on relatively narrow domains of composition which shows the reduced miscibility of the constituents. Finally, a structural model of one of the intermediate solid solutions is developed which well accounts for the mixing behaviour of the two fatty acids and permits to propose an explanation about their low solid-state miscibility.
Topics: Fatty Acids; Models, Molecular; Solubility; Solutions; Surface Properties; Temperature; X-Ray Diffraction
PubMed: 18505679
DOI: 10.1016/j.chemphyslip.2008.04.008