-
Polish Journal of Veterinary Sciences 2015Trichomonadidae family is a protozoan occurring in different animal species. It inhabits the gastrointestinal and urinary tracts. P. hominis is rarely found in faecal...
Trichomonadidae family is a protozoan occurring in different animal species. It inhabits the gastrointestinal and urinary tracts. P. hominis is rarely found in faecal samples of dogs, and its identification and differentiation from other trichomonads by light microscopy are difficult. Methods of molecular biology are the most effective in this case, because they confirm the presence of the specific species in animal organisms, irrespective of the protozoan form. The aim of this study was to find P. hominis in selected dog kennels in North-Eastern Poland. Forty-one faecal samples of dogs from 7 dog kennels were examined. The occurrence of P. hominis in 5 faecal samples of dogs with no symptoms of diarrhoea was the first one to be confirmed in Poland.
Topics: Animals; Dog Diseases; Dogs; Female; Protozoan Infections, Animal; Real-Time Polymerase Chain Reaction; Trichomonadida
PubMed: 26812819
DOI: 10.1515/pjvs-2015-0100 -
Journal of Parasitic Diseases :... Sep 2022() is a large intestinal flagellated protozoan infecting humans. Little is known about the epidemiology of in Egypt, its association with gastrointestinal symptoms and...
() is a large intestinal flagellated protozoan infecting humans. Little is known about the epidemiology of in Egypt, its association with gastrointestinal symptoms and the co-infection with other parasites. Demographic and clinical data were collected from 180 school-aged children. Parasitological examination of fecal samples was done using direct wet mount, formalin ethyl-acetate, Kato-Katz and cultivation on Jones' medium to detect and associated parasitic infection. The diagnosis of was confirmed using Giemsa stain and scanning electron microscopy. The prevalence of was 13.8% (25 out of 180 children). The prevalence of parasitic co-infection was significantly higher in infected (84%, 21 participants) than in non-infected children (56%, 87 participants). The presence of abdominal pain and diarrhea in infected children was higher than in non-infected children (84% and 32% vs. 76% and 18%), respectively. The difference was not statistically significant. This is the first report of in Egypt. The significant association between and other intestinal parasites need more investigations. Further studies are needed to understand the epidemiology and pathogenicity of .
PubMed: 36091265
DOI: 10.1007/s12639-022-01506-1 -
Avian Diseases Mar 2023Intestinal health plays a major role in profitable and efficient turkey production. Blackhead disease (histomoniasis) is caused by , an anaerobic protozoan parasite....
Intestinal health plays a major role in profitable and efficient turkey production. Blackhead disease (histomoniasis) is caused by , an anaerobic protozoan parasite. disrupts intestinal integrity and may cause systemic infection. Some field outbreaks of blackhead disease are associated with low morbidity and mortality, while in some instances, it may cause severe morbidity and mortality. In the current study, a presumptive diagnosis of blackhead disease was made based on the characteristic gross lesions in the liver and ceca. The cecal culture, PCR, and sequencing confirmed the presence of and . has been reported in enteritis cases of several other species, such as dogs, cats, and cattle. The impact of on intestinal health of turkeys has not previously been studied, and to the best of our knowledge, this is the first case report of concurrent and infection in turkeys.
Topics: Animals; Cattle; Dogs; Turkeys; Protozoan Infections, Animal; Poultry Diseases; Trichomonadida; Trichomonas; Disease Outbreaks; Cattle Diseases; Dog Diseases
PubMed: 37140122
DOI: 10.1637/aviandiseases-D-22-00054 -
Rheumatology (Oxford, England) Aug 2013
Topics: Adalimumab; Aged; Antibodies, Monoclonal, Humanized; Arthritis, Rheumatoid; Follow-Up Studies; Humans; Immunocompromised Host; Male; Metronidazole; Rare Diseases; Risk Assessment; Treatment Outcome; Trichomonas; Trichomonas Infections
PubMed: 23325039
DOI: 10.1093/rheumatology/kes385 -
Veterinary Parasitology Apr 2007Pentatrichomonas hominis is considered to be a commensal protozoan of the vertebrate digestive tract. On the basis of light microscopic examination of feces, some...
Pentatrichomonas hominis is considered to be a commensal protozoan of the vertebrate digestive tract. On the basis of light microscopic examination of feces, some investigators presumptively identified P. hominis as a causative agent of feline diarrhea. However, molecular identification of P. hominis infection in the cat has not been reported. Another trichomonad, Tritrichomonas foetus, is recognized as an intestinal pathogen in cats and often presumptively diagnosed on the basis of the presence of trichomonads in diarrheic feces. It is of importance to determine if cats are natural hosts for P. hominis, as the presence of this organism could result in inaccurate assumption of T. foetus infection. In this study, we used a species-specific PCR assay to identify P. hominis 18S rRNA genes in fecal samples collected from a convenience population of cats in which a high prevalence of T. foetus infection had been previously identified (cat show) or suspected (submitted for T. foetus diagnostic testing). The prevalence of T. foetus infection in these samples was 31% and 28.6%, respectively. P. hominis infection was identified by PCR of DNA extracted from feces of five cats (1.9% and 2.1% of fecal samples, respectively). All cats in which P. hominis was identified were also infected with T. foetus. PCR identification of P. hominis infection in the cat should facilitate future studies to determine the pathogenicity of this species and enable differentiation of P. hominis from other known or as-yet unidentified species of trichomonads that may infect cats.
Topics: Animals; Cat Diseases; Cats; Feces; Polymerase Chain Reaction; RNA, Protozoan; RNA, Ribosomal, 16S; RNA, Ribosomal, 18S; Trichomonadida; Trichomonas Infections
PubMed: 17127004
DOI: 10.1016/j.vetpar.2006.10.020 -
The Korean Journal of Parasitology Mar 2010Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The...
Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performed to confirm the P. hominis infection and its molecular characterization from the feces of puppies with diarrhea. Fecal samples were obtained from 14 German shepherd puppies with diarrhea over 1 week (7 females and 7 males, 2-9 months of age) residing on a dog farm in August 2007. Species-specific PCR assay identified P. hominis 18S rRNA genes in 3 of the 14 puppies (1 female and 2 males; 1 aged 2 months and 2 aged 9 months). This phylogenetic analysis established that P. hominis belonged to the 1st clade, which is comprised of Bos taurus and Felines.
Topics: Animals; Base Sequence; Cluster Analysis; DNA, Protozoan; DNA, Ribosomal; Diarrhea; Dog Diseases; Dogs; Feces; Female; Genes, rRNA; Male; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; Protozoan Infections, Animal; RNA, Protozoan; RNA, Ribosomal, 18S; Republic of Korea; Sequence Analysis, DNA; Sequence Homology; Trichomonadida
PubMed: 20333280
DOI: 10.3347/kjp.2010.48.1.9 -
Parasite (Paris, France) 2024Pentatrichomonas hominis, a flagellated parasitic protozoan, predominantly infects the mammalian digestive tract, often causing symptoms such as abdominal pain and...
Pentatrichomonas hominis, a flagellated parasitic protozoan, predominantly infects the mammalian digestive tract, often causing symptoms such as abdominal pain and diarrhea. However, studies investigating its pathogenicity are limited, and the mechanisms underlying P. hominis-induced diarrhea remain unclear. Establishing an in vitro cell model for P. hominis infection is imperative. This study investigated the interaction between P. hominis and IPEC-J2 cells and its impact on parasite growth, adhesion, morphology, and cell viability. Co-cultivation of P. hominis with IPEC-J2 cells resulted in exponential growth of the parasite, with peak densities reaching approximately 4.8 × 10 cells/mL and 1.2 × 10 cells/mL at 48 h for initial inoculation concentrations of 10 cells/mL and 10 cells/mL, respectively. The adhesion rate of P. hominis to IPEC-J2 cells reached a maximum of 93.82% and 86.57% at 24 h for initial inoculation concentrations of 10 cells/mL and 10 cells/mL, respectively. Morphological changes in IPEC-J2 cells co-cultivated with P. hominis were observed, manifesting as elongated and irregular shapes. The viability of IPEC-J2 cells exhibited a decreasing trend with increasing P. hominis concentration and co-cultivation time. Additionally, the mRNA expression levels of IL-6, IL-8, and TNF-α were upregulated, whereas those of CAT and CuZn-SOD were downregulated. These findings provide quantitative evidence that P. hominis can promote its growth by adhering to IPEC-J2 cells, inducing morphological changes, reducing cell viability, and triggering inflammatory responses. Further in vivo studies are warranted to confirm these results and enhance our understanding of P. hominis infection.
Topics: Animals; Trichomonas; Cell Proliferation; Abdominal Pain; Diarrhea; Gene Expression; Mammals
PubMed: 38530211
DOI: 10.1051/parasite/2024014 -
Antibiotics (Basel, Switzerland) Jul 2022Antimicrobial resistance (AMR) is becoming more common in both bacteria and pathogenic protozoa. Therefore, new solutions are being sought as alternatives to currently...
Antimicrobial resistance (AMR) is becoming more common in both bacteria and pathogenic protozoa. Therefore, new solutions are being sought as alternatives to currently used agents. There are many new ideas and solutions, especially compounds of natural origin, including essential oils. In the present study, the antiprotozoal activity of a mixture of essential oils (eucalyptus, lavender, cedar and tea tree), organic acids (acetic acid, propionic acid and lactic acid) and metal ions (Cu, Zn, Mn) were tested. As a model, protozoans were selected: , , , , . The tested concentrations of mixtures were in the range of 0.001-1.5%. The analyses show unexpected, very strong protozoicidal activity of combinations, presenting the synergy of compounds via determination of LD and LD values. Obtained mixtures showed significantly higher activity against protozoans, compared to chloramphenicol and metronidazole. Most of the analyzed samples show high antiprotozoal activity at very low concentration, in the range of 0.001-0.009%. The most effective combinations for all analyzed protozoans were the cedar essential oil and tea tree essential oil with a mixture of acids and manganese or zinc ions. Innovative combinations of essential oils, organic acids and metal ions are characterized by very high antiprotozoal activity at low doses, which, after further investigation, can be applicable for control of protozoan pathogens.
PubMed: 35884167
DOI: 10.3390/antibiotics11070913 -
Letters in Applied Microbiology 2004To develop a sensitive and specific polymerase chain reaction for the detection of Pentatrichomonas hominis in biological specimens.
AIM
To develop a sensitive and specific polymerase chain reaction for the detection of Pentatrichomonas hominis in biological specimens.
METHODS
Three primers, associated in two primer pairs, were designed to amplify a sequence from the SSU rRNA gene of P. hominis. The specificity of both primer pairs was established by testing DNA extractions of different Trichomonad species, protozoa, bacteria, yeasts, and human leucocytes. The analytical sensitivity was determined through testing dilutions of P. hominis trophozoites. The clinical specificity and applicability of the assay was evaluated on stool samples and self-administered vaginal swabs.
CONCLUSIONS
A highly specific and sensitive PCR assay was developed. Both primer pairs performed equally well.
SIGNIFICANCE AND IMPACT OF THE STUDY
The presence of P. hominis in vaginal specimens has not been reported before.
Topics: Animals; Bacteria; DNA, Protozoan; DNA, Ribosomal; Eukaryota; Feces; Female; Genes, rRNA; Humans; Leukocytes; Polymerase Chain Reaction; Sensitivity and Specificity; Trichomonadida; Vagina; Yeasts
PubMed: 15130148
DOI: 10.1111/j.1472-765X.2004.01528.x -
The Journal of Protozoology Aug 1968
Topics: Eukaryota; Humans; Intestines; Membranes; Microscopy, Electron; Organoids; Trichomonas
PubMed: 5703074
DOI: 10.1111/j.1550-7408.1968.tb02151.x