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Somatic Cell Genetics Nov 1978Forty-three independent variants of the Novikoff hepatoma cell line have been isolated for their ability to use D-xylose, D-ribose, and/or L-arabinose as a sole carbon...
Forty-three independent variants of the Novikoff hepatoma cell line have been isolated for their ability to use D-xylose, D-ribose, and/or L-arabinose as a sole carbon and energy source. The variants exhibited marked morphological changes and a loss or decrease of cloning efficiency in soft agar. The xylose and arabinose variants showed similar phenotypes while the ribose variants were a phenotypically heterogenous group. Two major classes of variants were found with regard to their specificity for pentoses: one class could grow on ribose, xylose, or arabinose, while the second class grew only on ribose. The lack of specificity for pentose use was correlated with the ability to use pentitols for growth. The frequency of pentose-utilizing clones was 5 X 10(-2) to 10(-3), and nitrosoguanidine treatment increased this frequency tenfold. Fluctuation analyses showed the appearance of pentose-utilizing variants to be a random event. Of the variants examined, 84% expressed a stable pentose phenotype, and of those, 6% were cold sensitive and 8% were temperature sensitive for pentose utilization. In addition to the suggested mutational basis for the pentose phenotype, two variants showed a large increase in chromosome number from 73 +/- 3 to 132 +/- 10.
Topics: Animals; Chromosomes; Clone Cells; Gene Frequency; Genetic Variation; Karyotyping; Liver Neoplasms, Experimental; Methylnitronitrosoguanidine; Mutation; Pentoses; Phenotype
PubMed: 570306
DOI: 10.1007/BF01543156 -
The Journal of Biological Chemistry Apr 1962
Topics: Candida; Carbohydrate Metabolism; L-Iditol 2-Dehydrogenase; NAD; Oxidoreductases; Pentoses
PubMed: 13877918
DOI: No ID Found -
Archives of Biochemistry and Biophysics Apr 1958
Topics: Oxidation-Reduction; Pentosephosphates; Pentoses; Phosphates
PubMed: 13534661
DOI: 10.1016/0003-9861(58)90002-x -
Science (New York, N.Y.) May 1950
Topics: Bacteremia; Gluconates; Pentoses; Phosphates; Saccharomyces cerevisiae; Yeasts
PubMed: 15418189
DOI: 10.1126/science.111.2890.543 -
Molecules and Cells Jun 1998When E. coli cells were grown in minimal medium supplemented with D-ribose and D-xylose, a diauxic growth preferring D-xylose was observed. Transcription of the ribose...
When E. coli cells were grown in minimal medium supplemented with D-ribose and D-xylose, a diauxic growth preferring D-xylose was observed. Transcription of the ribose (rbs) operon was repressed in the presence of D-xylose, phenotypically similar to catabolite repression by D-glucose, although D-ribose did not affect transcription of the xylose (xyl) operon. Complementation analysis with xylR revealed that the repression of the rbs operon by D-xylose is exerted at the transcriptional level through XylR, suggesting a novel mechanism for catabolite repression. Furthermore, it was shown that L-arabinose reduced transcriptions of both xyl and rbs operons, whereas the arabinose operon was not affected by D-xylose or D-ribose, suggesting a priority mechanism for pentose utilization.
Topics: Arabinose; Bacterial Proteins; Cell Division; DNA-Binding Proteins; Escherichia coli; Gene Expression Regulation, Bacterial; Genes, Bacterial; Operon; Pentoses; Repressor Proteins; Ribose; Transcription Factors; Transcription, Genetic; Xylose
PubMed: 9666469
DOI: No ID Found -
Carbohydrate Research Apr 2023A new synthesis of rare d-lyxose from easily available d-arabinose is disclosed. The route includes 7 steps with a total 40% yield. Inversion of configuration at C3...
A new synthesis of rare d-lyxose from easily available d-arabinose is disclosed. The route includes 7 steps with a total 40% yield. Inversion of configuration at C3 promoted by DAST reagent is utilized on trans-2,3-di-hydroxy pentofuranose to provide cis-2,3-di-hydroxy pentofuranose, which is hardly synthesized using normal method.
Topics: Arabinose; Pentoses
PubMed: 37001234
DOI: 10.1016/j.carres.2023.108782 -
American Journal of Human Genetics Sep 1962
Topics: Carbohydrate Metabolism, Inborn Errors; Humans; Metabolic Diseases; Pentoses; Sugar Alcohol Dehydrogenases; Xylulose
PubMed: 14455483
DOI: No ID Found -
British Medical Journal Feb 1955
Topics: Carbohydrate Metabolism, Inborn Errors; Humans; Pentoses; Sugar Alcohol Dehydrogenases; Urine; Xylulose
PubMed: 13230505
DOI: 10.1136/bmj.1.4910.391 -
Applied Microbiology and Biotechnology Mar 2018Functional sugars have attracted attention because of their wide application prospects in the food, cosmetics, and pharmaceutical industries in recent decades. Compared... (Review)
Review
Functional sugars have attracted attention because of their wide application prospects in the food, cosmetics, and pharmaceutical industries in recent decades. Compared with complex chemical synthesis, enzymatic methods of creating functional sugars, characterized by high specificity, moderate reaction conditions, and sustainability, are favored. D-lyxose isomerase (D-LI, EC 5.3.1.15), an important aldose-ketose isomerase, catalyzes the reverse isomerization reaction between D-xylulose and D-lyxose, as well as D-fructose and D-mannose. D-LI has drawn researchers' attention due to its broad substrate specificity and high potential for enzymatic production of some functional sugars such as D-xylulose, D-mannose, and D-ribose. In this article, an overview of recent advances in the biochemical properties of various D-LIs is explored in detail. Structural analysis, active site identification, and catalytic mechanisms are also provided. Additionally, the applications of D-LIs for functional sugar production, including D-lyxose, D-mannose, and L-ribose, are reviewed in detail in this paper.
Topics: Biocatalysis; Industrial Microbiology; Isomerases; Pentoses; Sugars
PubMed: 29392387
DOI: 10.1007/s00253-018-8746-6 -
Nature May 1954
Topics: Nucleic Acids; Pentoses
PubMed: 13165710
DOI: 10.1038/1731037a0