-
Anaerobe Dec 2021Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in... (Review)
Review
Peptostreptococcus anaerobius: Pathogenicity, identification, and antimicrobial susceptibility. Review of monobacterial infections and addition of a case of urinary tract infection directly identified from a urine sample by MALDI-TOF MS.
Peptostreptococcus anaerobius is a gram-positive anaerobic coccus (GPAC) found in the gastrointestinal and vaginal microbiota. The organism is mainly found in polymicrobial and scarcely in monobacterial infections such as prosthetic and native endocarditis. Anaerobic bacteria have rarely been reported as the cause of urinary tract infection (UTI). Although GPAC are susceptible to most antimicrobials used against anaerobic infections, P. anaerobius has shown to be more resistant. Herein, we report a case of UTI caused by P. anaerobius from a 62-year-old man with a history of urological disease. Surprisingly, the microorganism was directly identified by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) from the urine sample. The isolate was successfully identified by phenotypic methods, MALDI-TOF MS, and 16S rRNA gene sequencing. P. anaerobius showed no β-lactamase-producing activity, was resistant to penicillin, ampicillin, ciprofloxacin and levofloxacin, and displayed intermediate susceptibility to ampicillin-sulbactam and amoxicillin-clavulanic acid. Successful treatment was achieved with oral amoxicillin-clavulanic acid. Antimicrobial susceptibility testing (AST) should be performed on P. anaerobius isolates due to their unpredictable AST patterns and because empirically administered antimicrobial agents may not be active. This report shows that MALDI-TOF MS, directly used in urine specimens, may be a quick option to diagnose UTI caused by P. anaerobius or other anaerobic bacteria. This review is a compilation of monobacterial infections caused by P. anaerobius published in the literature, their pathogenicity, identification, and data about the antimicrobial susceptibility of P. anaerobius.
Topics: Anti-Infective Agents; Bacterial Typing Techniques; Disease Susceptibility; Gram-Positive Bacterial Infections; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Molecular Typing; Peptostreptococcus; RNA, Ribosomal, 16S; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Treatment Outcome; Urinary Tract Infections
PubMed: 34626800
DOI: 10.1016/j.anaerobe.2021.102461 -
Cellular and Molecular Biology... Oct 2023Accumulating evidences have shown that Peptostreptococcus anaerobius (P.a) is abundantly enriched in the fetus of colorectal cancer (CRC) patients. P.a is reported able...
Accumulating evidences have shown that Peptostreptococcus anaerobius (P.a) is abundantly enriched in the fetus of colorectal cancer (CRC) patients. P.a is reported able to invade colorectal tissues. This study intends to uncover the clinical significance of P.a in CRC. Mucosal tissues collected from CRC cases (n=109) and precancerous healthy ones (n=65) were subjected to the determination of the absolute copy number of P.a by droplet digital PCR. The positive rate of P.a in mucosal tissues of CRC and healthy ones was 79.8% (87/109), and 55.4% (36/65), respectively. The average absolute copy number of P.a in them was 2.3 copy/ng DNA, and 0.32 copy/ng DNA, respectively. The abundance of P.a in mucosal tissues of CRC, and age and TNM staging of CRC cases were correlated to its survival. The abundance of P.a in CRC cases was remarkably correlated to the relative level of SQLE. The abundance of P.a can be monitored to predict the prognosis of CRC.
Topics: Humans; Colorectal Neoplasms; Peptostreptococcus; Biomarkers; DNA; Biomarkers, Tumor
PubMed: 37953554
DOI: 10.14715/cmb/2023.69.10.36 -
Biochemical Society Transactions Jun 2003Protein L is a multidomain cell-wall protein isolated from Peptostreptococcus magnus. It belongs to a group of proteins that contain repeated domains that are able to... (Review)
Review
Protein L is a multidomain cell-wall protein isolated from Peptostreptococcus magnus. It belongs to a group of proteins that contain repeated domains that are able to bind to Igs without stimulating an immune response, the most characterized of this group being Protein A ( Staphylococcus aureus ) and Protein G ( Streptococcus ). Both of these proteins bind predominantly to the interface of C(H)2-C(H)3 heavy chains, while Protein L binds exclusively to the V(L) domain of the kappa -chain. The function of these proteins in vivo is not clear but it is thought that they enable the bacteria to evade the host's immune system. Two binding sites for kappa -chain on a single Ig-binding domain from Protein L have recently been reported and we give evidence that one site has a 25-55-fold higher affinity for kappa -chain than the second site.
Topics: Amino Acid Sequence; Antigens, Bacterial; Bacterial Proteins; Binding Sites; Epitopes; Immunoglobulins; Ligands; Molecular Sequence Data; Peptostreptococcus
PubMed: 12773190
DOI: 10.1042/bst0310716 -
Nihon Saikingaku Zasshi. Japanese... Mar 1987
Review
Topics: Cytosine; DNA, Bacterial; Fatty Acids; Guanine; Peptococcus; Peptostreptococcus
PubMed: 3302346
DOI: 10.3412/jsb.42.471 -
Anales de Medicina Interna (Madrid,... May 2008We describe two new cases of Peptostreptococcus endocarditis, one case of Peptostreptococcus micros prosthetic valve endocarditis and the other of Peptostreptococcus... (Review)
Review
We describe two new cases of Peptostreptococcus endocarditis, one case of Peptostreptococcus micros prosthetic valve endocarditis and the other of Peptostreptococcus assaccharolyticus tricuspid native valve endocarditis in an intravenous drug user (IDU) patient and review nine cases previously reported.
Topics: Adult; Aged; Endocarditis, Bacterial; Female; Gram-Positive Bacterial Infections; Humans; Male; Middle Aged; Peptostreptococcus; Young Adult
PubMed: 18769744
DOI: 10.4321/s0212-71992008000500006 -
International Journal of Systematic and... Aug 2011Using a polyphasic approach, a taxonomic study was performed on seven strains of an unknown Gram-reaction-positive, non-spore-forming, obligately anaerobic coccus-shaped...
Using a polyphasic approach, a taxonomic study was performed on seven strains of an unknown Gram-reaction-positive, non-spore-forming, obligately anaerobic coccus-shaped bacterium, isolated from a swine-manure storage pit. Comparative 16S rRNA gene sequencing confirmed that all seven isolates were highly related to each other and formed a hitherto unknown lineage within the clostridial rRNA XI cluster of organisms. Pairwise analysis demonstrated that the novel organism was most closely related to Peptostreptococcus anaerobius CCUG 7835(T) and Peptostreptococcus stomatis CCUG 51858(T) with 16S rRNA gene sequence similarities of 95.5 and 93.0 %, respectively. The peptidoglycan type of the cell wall was determined to be A4α l-Lys-d-Asp and glucose, xylose and traces of mannose were detected as the cell-wall sugars. Based on biochemical, chemotaxonomic and phylogenetic evidence the unknown bacterium represents a new species of the genus Peptostreptococcus, for which the name Peptostreptococcus russellii sp. nov, is proposed. The type strain is RT-10B(T) ( = CCUG 58235(T) = NRRL B-59380(T) = DSM 23041(T)).
Topics: Animals; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Manure; Molecular Sequence Data; Peptostreptococcus; Phylogeny; RNA, Ribosomal, 16S; Swine
PubMed: 20833884
DOI: 10.1099/ijs.0.023762-0 -
The Journal of Rheumatology Oct 1988We describe a 30-year-old female presenting with synovitis of the left 4th proximal interphalangeal joint. Anaerobic cultures of the synovial membrane grew... (Review)
Review
We describe a 30-year-old female presenting with synovitis of the left 4th proximal interphalangeal joint. Anaerobic cultures of the synovial membrane grew Peptostreptococcus magnus. The synovitis resolved after a course of intravenous penicillin G. The role of the anaerobic bacterium Peptostreptococcus magnus in joint infections is reviewed.
Topics: Adult; Arthritis, Infectious; Female; Finger Joint; Humans; Penicillin G; Peptostreptococcus
PubMed: 3060615
DOI: No ID Found -
Clinical Infectious Diseases : An... Dec 1997We describe a patient with postsurgical anaerobic meningitis due to Peptostreptococcus magnus. In cases of meningitis associated with Peptostreptococcus species reported... (Review)
Review
We describe a patient with postsurgical anaerobic meningitis due to Peptostreptococcus magnus. In cases of meningitis associated with Peptostreptococcus species reported in the literature, the most common predisposing factors are meningorectal fistulae and head-and-neck surgery. Most patients respond well to appropriate antimicrobial therapy. Surgical intervention may be required in some instances.
Topics: Female; Gram-Positive Bacterial Infections; Humans; Meningitis, Bacterial; Middle Aged; Peptostreptococcus
PubMed: 9431395
DOI: 10.1086/516147 -
Oral Microbiology and Immunology Feb 1992Peptostreptococcus micros is a recognized pathogen in medical infections, and its association with progressive periodontitis was examined in this study. P. micros was...
Peptostreptococcus micros is a recognized pathogen in medical infections, and its association with progressive periodontitis was examined in this study. P. micros was isolated from paper-point subgingival samples on anaerobic enriched blood agar plates and identified on the basis of cellular and colonial morphology and selected biochemical tests. In a cross-sectional study involving 907 people with advanced adult periodontitis, 127 with early-onset periodontitis, and 12 with localized juvenile periodontitis, P. micros in these patient groups occurred with a prevalence of 58-63%. In culture-positive patients, P. micros averaged 12-15% of total viable counts. P. micros demonstrated similar occurrence and proportional recovery in all age groups. In a longitudinal study of 91 adult periodontitis patients on maintenance therapy, P. micros demonstrated a significantly higher prevalence in disease-active than in disease-inactive patients (47% vs 14%). Mechanical subgingival debridement and 0.12% chlorhexidine pocket irrigation was unable to eradicate subgingival P. micros from 18 of 22 adult periodontitis patients. In vitro antimicrobial susceptibility testing showed P. micros to be sensitive to therapeutic levels of penicillin, clindamycin and metronidazole. Our findings indicate that P. micros is a potential pathogen in adult periodontitis. The methods for its eradication from subgingival sites remain to be determined.
Topics: Adult; Aged; Aged, 80 and over; Aggressive Periodontitis; Anti-Bacterial Agents; Chi-Square Distribution; Chlorhexidine; Clindamycin; Cross-Sectional Studies; Dental Scaling; Female; Humans; Longitudinal Studies; Male; Metronidazole; Microbial Sensitivity Tests; Middle Aged; Odds Ratio; Penicillins; Peptostreptococcus; Periodontitis; Predictive Value of Tests; Sensitivity and Specificity
PubMed: 1528618
DOI: 10.1111/j.1399-302x.1992.tb00011.x -
International Journal of Systematic and... Apr 2006Seven strains of anaerobic Gram-positive cocci isolated from human oral sites were subjected to a comprehensive range of phenotypic and genotypic tests. 16S rRNA gene...
Seven strains of anaerobic Gram-positive cocci isolated from human oral sites were subjected to a comprehensive range of phenotypic and genotypic tests. 16S rRNA gene sequence analysis revealed that the strains constituted a homogeneous group that was distinct from species with validly published names, but related to Peptostreptococcus anaerobius. All oral strains tested belonged to this group, whereas all non-oral strains studied were confirmed as P. anaerobius. A novel species, Peptostreptococcus stomatis sp. nov., is proposed to accommodate these oral strains. P. stomatis is weakly saccharolytic and produces acetic, butyric, isobutyric, isovaleric and isocaproic acids as end products of fermentation. The type strain of P. stomatis is W2278T (=DSM 17678T = CCUG 51858T); the G+C content of the DNA of this strain is 36 mol%.
Topics: Fermentation; Humans; Molecular Sequence Data; Mouth; Peptostreptococcus; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA
PubMed: 16585688
DOI: 10.1099/ijs.0.64041-0