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Arquivos Brasileiros de Oftalmologia 2008Acute dacryocystitis usually induces preseptal infection. In rare instances the infection that is confined to the lacrimal sac can extend to the orbital contents... (Review)
Review
Acute dacryocystitis usually induces preseptal infection. In rare instances the infection that is confined to the lacrimal sac can extend to the orbital contents resulting in orbital cellulitis. We present a case of intraconal abscess secondary to acute dacryocystitis and review the literature of orbital cellulitis resulting from acute lacrimal sac infection.
Topics: Abscess; Adult; Bacteroidaceae Infections; Dacryocystitis; Female; Gram-Positive Bacterial Infections; Humans; Orbital Diseases; Peptostreptococcus; Prevotella melaninogenica
PubMed: 18797671
DOI: 10.1590/s0004-27492008000400020 -
Infectious Diseases in Obstetrics and... 1998Transvaginal ultrasound-guided aspiration of ovarian endometrioma has been applied and emphasized as a safe and simple procedure.
BACKGROUND
Transvaginal ultrasound-guided aspiration of ovarian endometrioma has been applied and emphasized as a safe and simple procedure.
CASE
Two 27-year-old infertile women, both gravida 0, para 0, underwent medical follow-up examinations for cases of ovarian endometrioma. Both had undergone transvaginal ultrasound-guided aspiration of ovarian endometrioma. Because both were continuously febrile and had abdominal pain and cysts with tenderness in spite of antibiotic therapies, both underwent laparotomies for treatment. In both cases, enucleation of the ovarian abscess revealed purulent and malodorous fluid that demonstrated Peptostreptococcus magnus in culture.
CONCLUSION
We theorize that following transvaginal ultrasound-guided aspiration of ovarian endometrioma and fixation with pure ethanol, anaerobic infection by P. magnus occurred, and a cyst formed in the abscess.
Topics: Abscess; Adult; Endometriosis; Ethanol; Female; Gram-Positive Bacterial Infections; Humans; Ovarian Diseases; Peptostreptococcus; Suction; Ultrasonography; Vagina
PubMed: 9702588
DOI: 10.1002/(SICI)1098-0997(1998)6:2<66::AID-IDOG7>3.0.CO;2-5 -
Infection and Immunity Feb 1985Hyaluronidase was purified to apparent homogeneity from the spent medium of Peptostreptococcus sp. strain 84H14S. The enzyme was purified 310-fold by ethanol...
Hyaluronidase was purified to apparent homogeneity from the spent medium of Peptostreptococcus sp. strain 84H14S. The enzyme was purified 310-fold by ethanol precipitation, gel chromatography, and cation-exchange chromatography with a recovery of 42% of the original activity in the culture medium. The molecular weight of the purified enzyme was estimated to be 160,000 by gel filtration with Sephacryl S-300. Like bacterial mucopolysaccharidases of other sources, the enzyme carried out an eliminative reaction with the substrate, producing 4,5-unsaturated disaccharides as the final end products. Its optimum temperature of activity is 46 degrees C. The purified peptostreptococcal hyaluronidase was different from previously reported bacterial hyaluronidases in several respects. It degraded hyaluronic acid rapidly and also exhibited some activity against chondroitin sulfate A and chondroitin sulfate C. The KmS for hyaluronic acid, chondroitin sulfate A, and chondroitin sulfate C were 0.14, 1.4, and 2.6 mg/ml, respectively. The specific activity of hyaluronidase was much higher than that of any previously purified mucopolysaccharidases. The Vmax against hyaluronic acid reached 400 mmol of product per min per mg of protein at 22 degrees C. The peptostreptococcal hyaluronidase was also unique in that its optimum pH of activity was around neutrality, whereas other bacterial hyaluronidases were most active at acidic pHs.
Topics: Bacterial Proteins; Bacteriological Techniques; Chromatography, Gel; Hyaluronoglucosaminidase; Hydrogen-Ion Concentration; Molecular Weight; Peptostreptococcus; Substrate Specificity
PubMed: 3881352
DOI: 10.1128/iai.47.2.508-513.1985 -
Microbiology (Reading, England) Jul 2003Here, a rapid and reliable two-step multiplex PCR assay for identifying 14 Gram-positive anaerobic cocci (GPAC) species originally classified in the genus... (Comparative Study)
Comparative Study
Rapid identification of Gram-positive anaerobic coccal species originally classified in the genus Peptostreptococcus by multiplex PCR assays using genus- and species-specific primers.
Here, a rapid and reliable two-step multiplex PCR assay for identifying 14 Gram-positive anaerobic cocci (GPAC) species originally classified in the genus Peptostreptococcus (Anaerococcus hydrogenalis, Anaerococcus lactolyticus, Anaerococcus octavius, Anaerococcus prevotii, Anaerococcus tetradius, Anaerococcus vaginalis, Finegoldia magna, Micromonas micros, Peptostreptococcus anaerobius, Peptoniphilus asaccharolyticus, Peptoniphilus harei, Peptoniphilus indolicus, Peptoniphilus ivorii and Peptoniphilus lacrimalis) is reported. Fourteen type strains representing 14 GPAC species were first identified to the genus level by multiplex PCR (multiplex PCR-G). Since three of these genera (Finegoldia, Micromonas and Peptostreptococcus) contain only a single species, F. magna, M. micros and P. anaerobius, respectively, these organisms were identified to the species level directly by using the multiplex PCR-G. Then six species of the genus Anaerococcus (A. hydrogenalis, A. lactolyticus, A. octavius, A. prevotii, A. vaginalis and A. tetradius) were further identified to the species level using multiplex PCR assays (multiplex PCR-Ia and multiplex PCR-Ib). Similarly, five species of the genus Peptoniphilus (Pn. asaccharolyticus, Pn. harei, Pn. indolicus, Pn. ivorii and Pn. lacrimalis) were identified to the species level using multiplex PCR-IIa and multiplex PCR-IIb. The established two-step multiplex PCR identification scheme was applied to the identification of 190 clinical isolates of GPAC species that had been identified previously to the species level by 16S rRNA sequencing and phenotypic tests. The identification obtained from multiplex PCR assays showed 100 % agreement with 16S rDNA sequencing identification, but only 65 % (123/190) agreement with the identification obtained by phenotypic tests. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of GPAC species. It will permit a more accurate assessment of the role of various GPAC species in infection and of the degree of antimicrobial resistance in each of the group members.
Topics: Base Sequence; DNA Primers; DNA, Bacterial; DNA, Ribosomal; Gram-Positive Cocci; Peptostreptococcus; Polymerase Chain Reaction; RNA, Bacterial; RNA, Ribosomal, 16S; Sensitivity and Specificity; Species Specificity
PubMed: 12855723
DOI: 10.1099/mic.0.26227-0 -
Journal of Clinical Microbiology Oct 1979Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus...
Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonomic decision to transfer aerotolerant Peptostreptococcus species to the genus Streptococcus. Because inconsistencies in the results prevented our differentiating Peptococcus prevotii. Peptococcus magnus, and Peptococcus variabilis by GLC, additional strains will have to been examined. These GLC techniques are amenable to routine use; however, for interlaboratory results to be meaningful, the classification and nomenclature of the anaerobic gram-positive cocci should be standardized.
Topics: Amines; Chromatography, Gas; Fatty Acids; Fatty Acids, Volatile; Peptococcus; Peptostreptococcus; Species Specificity; Streptococcus
PubMed: 528680
DOI: 10.1128/jcm.10.4.464-476.1979 -
Journal of Endodontics Mar 1989Eubacterium, Peptostreptococcus, and Bacteroides were isolated in high frequency from root canals with acute periapical inflammation. The antimicrobial susceptibilities... (Comparative Study)
Comparative Study
Eubacterium, Peptostreptococcus, and Bacteroides were isolated in high frequency from root canals with acute periapical inflammation. The antimicrobial susceptibilities of these strains were studied by determining minimum inhibitory concentrations of different agents. Although all three kinds of isolates were susceptible to penicillins, the isolates other than black-pigmented Bacteroides were less susceptible to cephems, tetracyclines, and macrorides with several resistant strains. All strains were uniformly resistant to aminoglycosides. Some differences in susceptibilities were observed among species of Eubacterium and Peptostreptococcus, while penicillins were effective for both species. Black-pigmented Bacteroides showed good susceptibilities to all agents except for aminoglycosides. The susceptibility of Bacteroides gingivalis was superior to that of Bacteroides intermedius. There were many resistant strains in non-black-pigmented but not in black-pigmented Bacteroides isolates. Penicillins were the most effective for Eubacterium, Peptostreptococcus, and Bacteroides, indicating that penicillins are suitable for treatment of root canals with acute apical periodontitis.
Topics: Aminoglycosides; Bacteroides; Eubacterium; Humans; Microbial Sensitivity Tests; Penicillins; Peptostreptococcus; Periapical Diseases; Tetracyclines
PubMed: 2607278
DOI: 10.1016/S0099-2399(89)80130-X -
Oral Microbiology and Immunology Dec 2007Microorganisms of Peptostreptococcus micros are asaccharolytic, anaerobic gram-positive cocci that are frequently isolated from human oral sites such as periodontal...
BACKGROUND/AIMS
Microorganisms of Peptostreptococcus micros are asaccharolytic, anaerobic gram-positive cocci that are frequently isolated from human oral sites such as periodontal pockets. Preliminary study showed that several amino acids, including serine, enhanced slightly the growth of P. micros. Therefore, we investigated the degradation of serine and serine-containing oligopeptides.
METHODS
Metabolic end products were determined with high-performance liquid chromatography. The related enzymatic activities in cell-free extract were also assayed.
RESULTS
Washed P. micros degraded serine-tripeptides (Ser-Ser-Ser), and produced formate, pyruvate, acetate, and ammonia. They also degraded serinyl-tyrosine (Ser-Tyr) to the same products. Related enzymatic activities, such as serine dehydratase, pyruvate formate-lyase, formate dehydrogenase, pyruvate oxidoreductase, phosphate acetyltransferase, and acetate kinase, were detected in the cell-free extract, indicating that the organisms produced ATP in the serine metabolism.
CONCLUSION
P. micros utilized serine-containing oligopeptides as exogenous metabolic substrates rather than serine itself, and degraded Ser-Ser-Ser and Ser-Tyr to formate, pyruvate, acetate, and ammonia with ATP generation.
Topics: Acetate Kinase; Acetates; Acetyltransferases; Adenosine Triphosphate; Ammonia; Formate Dehydrogenases; Formates; Humans; L-Serine Dehydratase; Oligopeptides; Peptostreptococcus; Phosphate Acetyltransferase; Pyruvate Synthase; Pyruvic Acid; Serine; Tyrosine
PubMed: 17949340
DOI: 10.1111/j.1399-302X.2007.00374.x -
Biophysical Chemistry Dec 2002A mixed culture of ovine ruminal microbes metabolizes the macrocyclic pyrrolizidine alkaloids present in the plant Senecio jacobaea, including jacobine and...
A mixed culture of ovine ruminal microbes metabolizes the macrocyclic pyrrolizidine alkaloids present in the plant Senecio jacobaea, including jacobine and seneciphylline. Previous attempts to identify metabolites of these alkaloids have not been successful. The objective of this study was to compare the metabolism of pyrrolizidine alkaloids by a mixed culture of ovine ruminal microbes to the metabolism of pyrrolizidine alkaloids by the known organism Peptostreptococcus heliotrinreducens. P. heliotrinreducens metabolizes the pyrrolizidine alkaloids heliotrine and lasiocarpine to 7alpha-hydroxy-1-methylene-8alpha-pyrrolizidine and 7alpha-angelyl-1-methylene-8alpha-pyrrolizidine, respectively. This organism does not metabolize the pyrrolizidine alkaloids jacobine or seneciphylline. A mixed culture of ovine ruminal microbes also metabolized heliotrine and lasiocarpine to identical methylene compounds. This mixed culture also metabolized jacobine and seneciphylline, with the production of very low levels of the corresponding 1-methylene compounds. Samples were analyzed by TLC and GC/MS.
Topics: Animals; Chromatography, Thin Layer; Gas Chromatography-Mass Spectrometry; Peptostreptococcus; Pyrrolizidine Alkaloids; Rumen; Sheep
PubMed: 12488016
DOI: 10.1016/s0301-4622(02)00152-7 -
Archives of Microbiology Jul 1985The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the...
The determination of enzymatic activities in cell-free extracts of Acidaminococcus fermentans and Peptostreptococcus asaccharolyticus led to a refined scheme for the pathway of glutamate fermentation via (R)-2-hydroxyglutarate to acetate and butyrate. From the ratio of these products the amount of ATP generated by substrate level phosphorylation was calculated. Growth experiments with the organisms including Clostridium symbiosum and Clostridium tetanomorphum indicated that a sodium gradient contributed additional energy for growth. The high growth yields found in organisms containing the biotin dependent sodium pump glutaconyl-CoA decarboxylase could be reduced by the sodium ionophor monensin. In P. asaccharolyticus energy equivalent up to 0.6 mol ATP per mol of glutaconyl-CoA decarboxylated was conserved via the Na+ gradient. The data may explain the growth promoting effects of monensin in cattle.
Topics: Adenosine Triphosphate; Bacteria, Anaerobic; Carboxy-Lyases; Clostridium; Energy Metabolism; Fermentation; Furans; Glutamates; Kinetics; Monensin; Peptostreptococcus; Phosphorylation; Sodium
PubMed: 4037980
DOI: 10.1007/BF00447055 -
Journal of Clinical Microbiology Sep 2003Oral Peptostreptococcus isolates tentatively identified by conventional microbiological culture methods were identified to the species level by a combination of PCR...
Oral Peptostreptococcus isolates tentatively identified by conventional microbiological culture methods were identified to the species level by a combination of PCR amplification of 16S rRNA genes and restriction enzyme analysis of the amplified products. This method is a reliable and rapid alternative to conventional methods for identification of these bacterial species.
Topics: Fatty Acids, Volatile; Humans; Mouth; Peptostreptococcus; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; RNA, Ribosomal, 16S
PubMed: 12958299
DOI: 10.1128/JCM.41.9.4475-4479.2003