-
International Journal of Medical... Jan 2005Micromonas (Peptostreptococcus) micros is frequently associated with periodontal disease as well as respiratory, gastrointestinal and female genitourinary tract...
Micromonas (Peptostreptococcus) micros is frequently associated with periodontal disease as well as respiratory, gastrointestinal and female genitourinary tract infections, but only rarely has been reported as a pathogenic agent of prosthetic joint infections. Here we describe a case of a 63-year-old woman with prosthetic joint infection of total hip arthroplasty caused by the anaerobic species Micromonas micros, associated with tooth extraction. Samples obtained intraoperatively and from the oral cavity were positive for the presence of M. micros by culture and by real-time PCR. This case report indicates that infections of prosthetic joints can be associated with dental procedures and that sensitive molecular techniques are necessary for their routine diagnostic.
Topics: Aged; Female; Gram-Positive Bacterial Infections; Hip Prosthesis; Humans; Joint Diseases; Peptostreptococcus; Prosthesis-Related Infections; Tooth Extraction
PubMed: 15715175
DOI: 10.1016/j.ijmm.2004.10.001 -
Journal of Endodontics Feb 2003A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect Peptostreptococcus micros in primary root canal infections. Samples were collected from 50...
A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect Peptostreptococcus micros in primary root canal infections. Samples were collected from 50 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. DNA extracted from the samples was amplified using the PCR assay, which yielded a specific fragment of P. micros 16S rDNA. P. micros was detected in 6 of 22 root canals associated with asymptomatic chronic periradicular lesions (27.3%), 2 of 8 teeth with acute apical periodontitis (25%), and 6 of 20 cases of acute periradicular abscess (30%). In general, P. micros was found in 14 of 50 cases (28%). There was no correlation between the presence of P. micros and the occurrence of symptoms. Findings suggested that P. micros can be involved in the pathogenesis of different forms of periradicular lesions.
Topics: Adolescent; Adult; Bacterial Typing Techniques; Chi-Square Distribution; DNA, Bacterial; Dental Pulp Necrosis; Gram-Positive Bacterial Infections; Humans; Middle Aged; Odds Ratio; Peptostreptococcus; Periapical Periodontitis; Polymerase Chain Reaction; RNA, Ribosomal, 16S
PubMed: 12597709
DOI: 10.1097/00004770-200302000-00006 -
American Journal of Ophthalmology May 1978A 46-year-old man developed symptoms of a chronic progressive uveitis in his right eye approximately one week after a dental procedure. The patient's intraocular...
A 46-year-old man developed symptoms of a chronic progressive uveitis in his right eye approximately one week after a dental procedure. The patient's intraocular inflammation was not diminished by massive treatment with topical and systemic corticosteroid therapy or intravenously administered adrenocorticotropic hormone. The inflammatory process progressed to an overt endophthalmitis during a period of three weeks and the eye eventually required evisceration. A pure culture of Peptostreptococcus intermedius was isolated from the eye. The most likely source of this organism was hematologic transport following a dental procedure.
Topics: Bacterial Infections; Endophthalmitis; Focal Infection, Dental; Humans; Male; Middle Aged; Peptostreptococcus; Periodontal Abscess; Periodontitis; Postoperative Complications
PubMed: 306760
DOI: 10.1016/s0002-9394(14)77101-6 -
Oral Microbiology and Immunology Feb 1993There are high amounts of hydrogen sulfide in deep periodontal pockets. This volatile sulfur compound may be formed from L-cysteine, but only low levels of this amino...
There are high amounts of hydrogen sulfide in deep periodontal pockets. This volatile sulfur compound may be formed from L-cysteine, but only low levels of this amino acid can be expected to be present in periodontal pockets. Glutathione, L-gamma-glutamyl-L-cysteinylglycine, is in high concentration in most tissue cells, and this tripeptide may be more readily available as a source of hydrogen sulfide formation in the pockets. The ability of 37 different species of oral bacteria to utilize glutathione in hydrogen sulfide formation was studied. Of these species, only 2 species of Peptostreptococcus and 5 species of Fusobacterium formed high amounts of hydrogen sulfide from glutathione within 24 h. Since the initial rate of hydrogen sulfide formation was more than 5 times higher in Peptostreptococcus micros than in any of the other bacterial species, the kinetics of sulfide formation from glutathione by P. micros was further elucidated. The formation of sulfide followed quite closely hyperbolic Michaelis-Menten kinetics. The maximal initial rate of sulfide formation (Vmax) was 163 +/- 2 nmol sulfide per minute per milligram of cellular protein. Half maximal initial rate (Km) was obtained at 7.4 +/- 0.8 microM glutathione. The initial rate of sulfide formation from L-cysteine was much slower and was almost proportional to L-cysteine concentration. This difference in kinetics of sulfide formation between glutathione and L-cysteine strongly suggested that glutathione was actively transported into the cell, whereas the transport of L-cysteine was more or less controlled by diffusion. The sulfide formation from the dipeptide L-cysteinylglycine also followed quite closely hyperbolic Michaelis-Menten kinetics.(ABSTRACT TRUNCATED AT 250 WORDS)
Topics: Biological Transport, Active; Cysteine; Dipeptides; Glutathione; Hydrogen Sulfide; Peptostreptococcus
PubMed: 8510983
DOI: 10.1111/j.1399-302x.1993.tb00541.x -
Applied and Environmental Microbiology May 1984Anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with B-vitamins and 0.05% yeast extract and with a 50% CO-30%... (Comparative Study)
Comparative Study
Anaerobic bacteria were enriched with a sewage digestor sludge inoculum and a mineral medium supplemented with B-vitamins and 0.05% yeast extract and with a 50% CO-30% N2-20% CO2 (2 atm [202 kPa]) gas phase. Microscopic observation revealed an abundance of gram-positive cocci, 1.0 by 1.4 micron, which occurred in pairs or chains. The coccus, strain U-1, was isolated by using roll tubes with CO as the energy source. Based on morphology, sugars fermented, fermentation products from glucose (H2, acetate, lactate, and succinate), and other features, strain U-1 was identified as Peptostreptococcus productus IIb (similar to the type strain). The doubling time with up to 50% CO was 1.5 h; acetate and CO2 were the major products. In addition, no significant change in the doubling time was observed with 90% CO. Some stock strains were also able to use CO, although not as well. Strain U-1 produced acetate during growth with H2-CO2. Other C1 compounds did not support growth. Most probable numbers of CO utilizers morphologically identical with strain U-1 were 7.5 X 10(6) and 1.1 X 10(5) cells per g for anaerobic digestor sludge and human feces, respectively.
Topics: Acetates; Acetic Acid; Anaerobiosis; Carbon Dioxide; Carbon Monoxide; Energy Metabolism; Hydrogen; Peptostreptococcus; Sewage
PubMed: 6430231
DOI: 10.1128/aem.47.5.961-964.1984 -
Antimicrobial Agents and Chemotherapy Aug 1991Of 15 Peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the Tet K, Tet M, or Tet O determinant. A donor Peptostreptococcus anaerobius...
Of 15 Peptostreptococcus sp. strains isolated between 1975 and 1984, 13 hybridized with the Tet K, Tet M, or Tet O determinant. A donor Peptostreptococcus anaerobius strain carrying the three determinants could transfer Tet M to P. anaerobius and Fusobacterium nucleatum recipients but not to an Enterococcus faecalis recipient, while neither Tet K nor Tet O was transferred.
Topics: Peptostreptococcus; Tetracycline Resistance
PubMed: 1929341
DOI: 10.1128/AAC.35.8.1682 -
International Journal of Systematic... Jul 1990We describe Peptostreptococcus hydrogenalis sp. nov., which is found in human feces and vaginal discharge. This new species was established on the basis of the results...
We describe Peptostreptococcus hydrogenalis sp. nov., which is found in human feces and vaginal discharge. This new species was established on the basis of the results of DNA-DNA hybridization among anaerobic cocci. The results of different biochemical reactions also are given. The type strain of this species is strain GIFU 7662 (= JCM 7635).
Topics: Base Composition; DNA, Bacterial; Feces; Female; Humans; Nucleic Acid Hybridization; Peptostreptococcus; Phenotype; Vagina
PubMed: 2397199
DOI: 10.1099/00207713-40-3-305 -
The Journal of International Medical... 2000We examined the characteristics of Peptostreptococcus species in infectious skin diseases. P. magnus was the species identified most frequently, followed by P....
We examined the characteristics of Peptostreptococcus species in infectious skin diseases. P. magnus was the species identified most frequently, followed by P. asaccharolyticus. Peptostreptococcus species were mainly isolated from infected atheroma and secondary infections due to ulcers; their resistance to five antimicrobial agents was generally low. The resistance of the three predominant Peptostreptococcus species to the antimicrobials was similar to that of all of the Peptostreptococcus species. The predominant Peptostreptococcus species isolated from infected atheroma might be pathogenic. It was considered that the presence of anaerobes as well as aerobes was important to induce the infectious condition.
Topics: Adolescent; Adult; Aged; Child; Child, Preschool; Female; Gram-Positive Bacterial Infections; Humans; Infant; Male; Middle Aged; Peptostreptococcus; Skin Diseases, Bacterial
PubMed: 10983864
DOI: 10.1177/147323000002800305 -
British Journal of Biomedical Science Dec 1996The current knowledge is reviewed concerning the anaerobic cocci, in particular those of clinical relevance. The anaerobic cocci are defined and their current taxonomic... (Review)
Review
The current knowledge is reviewed concerning the anaerobic cocci, in particular those of clinical relevance. The anaerobic cocci are defined and their current taxonomic positions discussed. It is clear that new genera and species await to be characterised fully. The overwhelming majority found in clinical material belong to the genus Peptostreptococcus, with the remainder belonging to the veillonellae and, possibly, ruminococci. Human infections with other anaerobic cocci are extremely rare. Their morphology, metabolism and culture, and role in clinical infections are assessed. The methods for isolation and identification, which for some species are difficult, are presented, together with brief summaries of the clinically important species. The review concludes with the current status of antibiotic susceptibilities and the methods used to test susceptibility in vitro. There is no current consensus as to which susceptibility test method is the method of choice.
Topics: Gram-Negative Anaerobic Cocci; Gram-Positive Cocci; Humans; Microbial Sensitivity Tests; Peptostreptococcus
PubMed: 9069108
DOI: No ID Found -
International Endodontic Journal Jan 2015To investigate whether DNA from two obligate anaerobes, Fusobacterium nucleatum and Peptostreptococcus anaerobius, is recoverable after loss of cell viability induced by...
AIM
To investigate whether DNA from two obligate anaerobes, Fusobacterium nucleatum and Peptostreptococcus anaerobius, is recoverable after loss of cell viability induced by air exposure.
METHODOLOGY
Harvested cultures of F. nucleatum and P. anaerobius were killed by exposure to air and stored in phosphate-buffered saline. Dead cells were incubated aerobically for up to 6 months. Every month, the presence of detectable DNA in the cell pellet and supernatant was assessed by conventional and quantitative PCR. Cell staining techniques were used to characterize the cell wall permeability of air-killed cells. Scanning electron microscopy was used to examine viable, freshly killed and stored cells.
RESULTS
With conventional PCR, amplifiable DNA was detectable over 6 months in all samples. Quantitative PCR showed a progressive fall in DNA concentration in nonviable cell pellets and a concomitant rise in DNA concentration in the supernatant. DNA staining showed that some air-killed cells retained an intact cell wall. After storage, SEM of both air-killed species revealed shrivelling of the cells, but some cells of P. anaerobius retained their initial form.
CONCLUSION
Amplifiable DNA from F. nucleatum and P. anaerobius was detectable 6 months after loss of viability. Air-killed anaerobes initially retained their cell form, but cells gradually shriveled over time. The morphological changes were more pronounced with the gram-negative F. nucleatum than the gram-positive P. anaerobius. Over 6 months, there was a gradual increase in cell wall permeability with progressive leakage of DNA. Bacterial DNA was recoverable long after loss of cell viability.
Topics: Air; Azides; DNA, Bacterial; Electrophoresis, Agar Gel; Fusobacterium nucleatum; Humans; Microscopy, Electron, Scanning; Necrosis; Peptostreptococcus; Polymerase Chain Reaction; Propidium; Staining and Labeling
PubMed: 24611977
DOI: 10.1111/iej.12273