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Carbohydrate Research Oct 1990The cell-envelope antigens of Peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. The purified species-specific antigen...
The cell-envelope antigens of Peptostreptococcus anaerobius were extracted from intact cells by autoclave or alkaline treatment. The purified species-specific antigen (G) was identified among several polysaccharides obtained from the extracts by successive treatments with ribonuclease and pronase followed by ion-exchange and gel-filtration chromatography. G was investigated by 13C- and 31P-n.m.r. spectroscopy, titrimetry, elemental analysis, and gas-liquid chromatography. Oxidation of G with NaIO4 followed by reduction with NaBH4 and mild acid hydrolysis yielded the Smith degradation product of G (GS). Treatment of G and GS with 48% HF gave the respective dephosphorylated products GF and GSF. The structures of GS, GF, and GSF were investigated by 13C-n.m.r. spectroscopy, methylation analysis, and gas-liquid chromatography-mass spectrometry. The principal constituents of G were 2-acetamido-2-deoxy-D-glucose (D-GlcNAc), D-glyceric acid, and phosphate as a diester, in the ratio 2:1:1, and a minor amount of D-glucose (beta-D-Glcp). GS contained D-GlcNAc, D-glyceric acid, glycerol, and phosphate in a 1:1:1:1 ratio. GF and GSF contained D-GlcNAc and D-glyceric acid in the ratios 2:1 and 1:1, respectively. A structure for the principal repeating unit of polymeric G compatible with the analytical data consists of alpha-D-GlcpNAc-(1----3)-alpha-D-GlcpNAc-(1----2)-D-glyceric acid units linked through C-6'-C-6" phosphate diester bridges. This structure is novel for two reasons: (a) unsubstituted glyceric acid residues occur as aglycons in the repeating structure, and (b) phosphate diester bridges link nonanomeric glycose carbons in a non-nucleic acid polymer. The structural role of the minor amount of beta-D-Glcp in G remains unknown.
Topics: Antigens, Bacterial; Carbohydrate Conformation; Carbohydrate Sequence; Chromatography, Gel; Chromatography, Ion Exchange; Hydrolysis; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Peptostreptococcus; Polysaccharides, Bacterial; Species Specificity
PubMed: 2076510
DOI: 10.1016/0008-6215(90)80009-r -
Journal of Immunology Research 2022As a common female reproductive system malignancy, cervical cancer (CC) disturbs numerous women's health. This study demonstrates the role of the vaginal microbial...
As a common female reproductive system malignancy, cervical cancer (CC) disturbs numerous women's health. This study demonstrates the role of the vaginal microbial environment () in cervical cancer. Functional assays, including cell proliferation assay, tube formation assay, and immunofluorescence staining, revealed the effect of -treated macrophages on cell proliferation and the angiogenesis process. The tube formation assay disclosed the function of -treated macrophages on angiogenesis. In vivo assays were also established to explore the impact of -treated macrophages on tumor migration. The results revealed that -induced macrophages boosted cervical cancer migration and angiogenesis both in vitro and in vivo. Then, this study unveiled that -induced macrophage secreted VEGF to stimulate the angiogenesis in cervical cancer. As a whole, -induced macrophage facilitates cervical cancer development through modulation of VEGF expression.
Topics: Female; Humans; Macrophages; Peptostreptococcus; Uterine Cervical Neoplasms; Vascular Endothelial Growth Factor A
PubMed: 35983073
DOI: 10.1155/2022/3525735 -
The Journal of Biological Chemistry May 1974
Topics: Adenosine Triphosphate; Amino Acids; Ammonium Sulfate; Apoproteins; Bacterial Proteins; Butyrates; Chromatography, DEAE-Cellulose; Chromatography, Gel; Coenzyme A; Electron Transport; Flavin-Adenine Dinucleotide; Flavoproteins; Macromolecular Substances; Molecular Weight; NAD; Oxidation-Reduction; Oxidoreductases; Peptostreptococcus; Riboflavin
PubMed: 4364030
DOI: No ID Found -
Journal of General Microbiology Sep 1984Glutamate dehydrogenase (L-glutamate:NAD+ oxidoreductase (deaminating); EC 1.4.1.2) has been purified from Peptostreptococcus asaccharolyticus in a single step using...
Glutamate dehydrogenase (L-glutamate:NAD+ oxidoreductase (deaminating); EC 1.4.1.2) has been purified from Peptostreptococcus asaccharolyticus in a single step using dye-ligand chromatography. The enzyme (GDH) was present in high yields and was stabilized in crude extracts. A subunit molecular weight of 49000 +/- 500 was determined by SDS polyacrylamide gel electrophoresis and six bands were obtained after cross-linking the subunits with dimethyl suberimidate. This bacterial GDH was predominantly NAD+-linked, but was able to utilize both NADP+ and NADPH at 4% of the rates with NAD+ and NADH, respectively. An investigation of the amino acid specificity revealed some similarities with GDH from mammalian sources and some clear differences. The values of apparent Km for the substrates ammonia, 2-oxoglutarate, NADH, NAD+ and glutamate were 18.4, 0.82, 0.066, 0.031 and 6 mM, respectively. The P. asaccharolyticus GDH was not regulated by purine nucleotides, but was subject to strong inhibition with increasing ionic strength.
Topics: Chromatography, Agarose; Coloring Agents; Glutamate Dehydrogenase; Kinetics; Macromolecular Substances; Molecular Weight; Osmolar Concentration; Peptostreptococcus; Substrate Specificity; Triazines
PubMed: 6502134
DOI: 10.1099/00221287-130-9-2385 -
Pathologie-biologie Jun 1998We report a retrospective study of 14 Peptostreptococcus magnus bone and joint infections, following orthopaedic prostheses or implantation of fixation devices,...
We report a retrospective study of 14 Peptostreptococcus magnus bone and joint infections, following orthopaedic prostheses or implantation of fixation devices, diagnosed in two Paris hospitals between 1992 and 1996. Five patients experienced a knee joint infection after anterior cruciate ligament reconstruction with 4 artificial grafts, and 9 caught joint or wound infections, after limb traumatic injuries or bone neoplastic ruptures involving femur, tibia, calcaneum and humerus, treated by arthroplasty or osteosynthesis with implantation of biomaterials. Septic arthritis was experienced one week to one year after reconstructive surgery, and had evolved for several months to years before etiologic diagnosis in 5 cases. Specimens of pus, tissues or removed implants produced numerous slow growing small colonies of Gram positive cocci arranged in clumps on culture media incubated in anaerobic atmosphere only. In 10 patients, the same organism was disclosed in several separate specimens. The identification of P. magnus was assessed by the enzyme profile (rapid ID 32A API strips), gaz liquid chromatography, catalase and coagulase production, resistance to novobiocin and Na polyanethol sulphonate. Antibiotic sensitivity testing performed by disc method was constant to penicillin G, amoxicillin, cefuroxime, cefoxitin, imipenem and pristinamycin with penicillin G MICs < 0.125 mg/l and metronidazole MICs < 1 mg/l. Erythromycin, clindamycin, rifampicin, tetracycline and fosfomycin were active against more than 70% of P. magnus. All patients were cured after a prolonged course of various antibiotics and surgical removal of the foreign material whenever possible. We studied in vitro binding of P. magnus with extracellular matrix proteins adsorbed onto biomaterials, by particle agglutination assays of latex beads coated with proteins. Eighty one% of strains bound to collagen, 69% to fibrinogen and 46% to fibronectin. Comparison of orthopaedic strains with strains of other infections and from skin showed a correlation between P. magnus from bone and joint infections and their fibrinogen binding ability (69% against 20%, p < 0.05).
Topics: Adolescent; Adult; Aged; Aged, 80 and over; Anti-Bacterial Agents; Arthritis, Infectious; Bone Diseases, Infectious; Drug Resistance, Microbial; Drug Therapy, Combination; Female; Gram-Positive Bacterial Infections; Humans; Incidence; Latex Fixation Tests; Male; Middle Aged; Orthopedic Procedures; Paris; Peptostreptococcus; Surgical Wound Infection
PubMed: 9769879
DOI: No ID Found -
Annals of the New York Academy of... Jul 1965
Topics: Acrylates; Animals; Azides; Coenzyme A; Dinitrophenols; Fermentation; Hydroxylamines; In Vitro Techniques; Intestinal Mucosa; Lactates; Peptostreptococcus; Pyruvates; Rumen
PubMed: 5216438
DOI: 10.1111/j.1749-6632.1965.tb47460.x -
Antimicrobial Agents and Chemotherapy Feb 2001Eighty percent (21 of 26) of macrolide-resistant Peptostreptococcus strains studied harbored the ermTR gene. This methyltransferase gene is also the most frequently...
Eighty percent (21 of 26) of macrolide-resistant Peptostreptococcus strains studied harbored the ermTR gene. This methyltransferase gene is also the most frequently found gene among macrolide-lincosamide-streptogramin B-resistant Streptococcus pyogenes strains. Transfer of the ermTR gene from Peptostreptococcus magnus to macrolide-susceptible S. pyogenes strains indicates that this resistance determinant may circulate among gram-positive aerobic and anaerobic species of the oropharyngeal bacterial flora.
Topics: Anti-Bacterial Agents; Bacterial Proteins; Conjugation, Genetic; Drug Resistance, Microbial; Electrophoresis, Agar Gel; Genes, Bacterial; Humans; Lincosamides; Macrolides; Methyltransferases; Peptostreptococcus; Reverse Transcriptase Polymerase Chain Reaction; Streptococcal Infections; Streptococcus pyogenes; Virginiamycin
PubMed: 11158770
DOI: 10.1128/AAC.45.2.630-632.2001 -
Molecular Oncology May 2024Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non-CRC) were collected for microbiome analysis. Saliva (28...
Oral and intestinal samples from a cohort of 93 colorectal cancer (CRC) patients and 30 healthy controls (non-CRC) were collected for microbiome analysis. Saliva (28 non-CRC and 94 CRC), feces (30 non-CRC and 97 CRC), subgingival fluid (20 CRC), and tumor tissue samples (20 CRC) were used for 16S metabarcoding and/or RNA sequencing (RNAseq) approaches. A differential analysis of the abundance, performed with the ANCOM-BC package, adjusting the P-values by the Holm-Bonferroni method, revealed that Parvimonas was significantly over-represented in feces from CRC patients (P-value < 0.001) compared to healthy controls. A total of 11 Parvimonas micra isolates were obtained from the oral cavity and adenocarcinoma of CRC patients. Genome analysis identified a pair of isolates from the same patient that shared 99.2% identity, demonstrating that P. micra can translocate from the subgingival cavity to the gut. The data suggest that P. micra could migrate in a synergistic consortium with other periodontal bacteria. Metatranscriptomics confirmed that oral bacteria were more active in tumor than in non-neoplastic tissues. We suggest that P. micra could be considered as a CRC biomarker detected in non-invasive samples such as feces.
Topics: Humans; Colorectal Neoplasms; Male; Female; Adenocarcinoma; Middle Aged; Aged; Mouth; Feces; RNA, Ribosomal, 16S; Gingiva; Saliva; Peptostreptococcus; Firmicutes
PubMed: 37558206
DOI: 10.1002/1878-0261.13506 -
Medical Hypotheses Aug 2012Finegoldia magna is an anaerobic Gram positive coccus, previously classified as Peptostreoptococcus magnus. It is normal flora of the gastrointestinal and genitourinary...
Finegoldia magna is an anaerobic Gram positive coccus, previously classified as Peptostreoptococcus magnus. It is normal flora of the gastrointestinal and genitourinary tract, and can be isolated from skin and the oral cavity and is often regarded as a contaminant in cultures. As the most frequently isolated anaerobic coccus, it is implicated in a range of mono- and polymicrobial infections, including skin and skin structure, bone and joint (native and prosthetic joints), infective endocarditis (native and prosthetic valves), necrotizing pneumonia, mediastinitis and meningitis. Recently, whole genome sequencing furthered the understanding of the pathogenicity of this organism by elucidating both chromosomally encoded and mobile plasmid mediated virulence factors. Although no cases of toxic shock syndrome have been attributed to F. magna, we present a case of a fatal monomicrobial F. magna bacteremia and hypothesize that superantigen activity, mediated via Protein L binding the variable domain of the κ light chains of IgG, resulted in the syndrome observed in our patient. Additionally, we suspect the overall significance of this pathogen is underestimated and with more sensitive detection methods, this organism will be identified more frequently in clinical cultures and associated with true infection.
Topics: Aged; Female; Gram-Positive Bacterial Infections; Humans; Models, Biological; Peptostreptococcus; Shock, Septic
PubMed: 22571938
DOI: 10.1016/j.mehy.2012.04.013 -
Journal of Medical Microbiology Dec 2002Peptostreptococcus anaerobius is a gram-positive anaerobic coccus that is widely distributed in the normal human flora. The organism has also been implicated as a...
Peptostreptococcus anaerobius is a gram-positive anaerobic coccus that is widely distributed in the normal human flora. The organism has also been implicated as a causative agent of several systemic infections, including endocarditis and infections of the genitourinary and gastrointestinal tracts. Its role in oral disease is less well defined, although it has been implicated in periodontal disease, gingivitis and root canal infections. Identification of P. anaerobius in clinical samples is currently reliant upon traditional culture and biochemical methods. The aim of this study was to develop a novel PCR assay for the detection of P. anaerobius and to attempt detection of this organism in oral samples. PCR primers specific for P. anaerobius DNA were developed by alignment of bacterial 16S ribosomal RNA gene sequences and selection of sequences specific at their 3' ends for P. anaerobius. When used in a PCR assay, positivity for P. anaerobius DNA was indicated by the amplification of a 943-bp product. The primers were shown to be specific for P. anaerobius DNA, as no PCR products were obtained when genomic DNA from a wide range of other Peptostreptococcus species and other oral bacteria were used as templates. The PCR assay was then applied to the detection of P. anaerobius DNA in subgingival plaque samples from adult periodontitis patients and pus aspirates from subjects with acute dento-alveolar abscesses. All of 60 subgingival plaque samples from 16 patients were negative for P. anaerobius DNA. None of the 43 pus samples analysed contained P. anaerobius DNA. These results suggest that P. anaerobius is not a major pathogen in adult periodontitis and dento-alveolar abscesses. The PCR assay is a more rapid, sensitive and specific alternative to culture-based methods for identification of P. anaerobius in clinical samples.
Topics: DNA Primers; DNA, Bacterial; Dental Plaque; Gene Amplification; Humans; Peptostreptococcus; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Sensitivity and Specificity; Sequence Alignment; Species Specificity; Time Factors
PubMed: 12466408
DOI: 10.1099/0022-1317-51-12-1097