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Oral Microbiology and Immunology Jun 2004This study characterized the microbial interaction of Peptostreptococcus micros and Prevotella intermedia, the major pathogens of dentoalveolar infection, using a murine... (Comparative Study)
Comparative Study
This study characterized the microbial interaction of Peptostreptococcus micros and Prevotella intermedia, the major pathogens of dentoalveolar infection, using a murine model. Subcutaneous injection of P. micros cells in the dorsum of the mouse together with living cells of P. intermedia resulted in a significantly larger abscess when compared with single injection of the organisms (P < 0.02). The abscess size was also significantly increased (P < 0.05) when the plate-cultured cell suspension of P. micros was injected into mouse with the culture filtrate of P. intermedia. The heat-treated culture filtrate of P. intermedia also enhanced the virulence of P. micros. P. micros culture filtrate did not affect the virulence of P. intermedia. Interestingly, the virulence of P. micros appeared to be enhanced even when the culture filtrate of P. intermedia was injected at separate sites in the mouse. These results suggest that a heat-stable product or products of P. intermedia increase the virulence of P. micros indirectly by altering the host condition, whereas living cells of P. micros can directly enhance virulence of P. intermedia.
Topics: Abscess; Animals; Bacteroidaceae Infections; Culture Media, Conditioned; Disease Models, Animal; Female; Gram-Positive Bacterial Infections; Mice; Mice, Inbred ICR; Peptostreptococcus; Prevotella intermedia; Skin Diseases, Bacterial; Virulence
PubMed: 15107069
DOI: 10.1111/j.0902-0055.2004.00138.x -
Trends in Biochemical Sciences Aug 1993Two families of enzymes are described which catalyse identical chemical reactions but differ in their prosthetic groups and hence in their mechanism of action. One... (Review)
Review
Two families of enzymes are described which catalyse identical chemical reactions but differ in their prosthetic groups and hence in their mechanism of action. One family, the pyridoxal-5'-phosphate (PLP)-dependent L-threonine dehydratases, also use L-serine as substrate. The other, hitherto unrecognized family is the iron-dependent, highly specific bacterial L-serine dehydratases. It has been shown that L-serine dehydratase from the anaerobic bacterium Peptostreptococcus asaccharolyticus contains an iron-sulfur cluster but no PLP. A mechanism for the dehydration of L-serine which is similar, but not identical, to that of the dehydration of citrate catalysed by aconitase is proposed.
Topics: Iron-Sulfur Proteins; L-Serine Dehydratase; Peptostreptococcus; Pyridoxal Phosphate; Substrate Specificity; Threonine Dehydratase
PubMed: 8236444
DOI: 10.1016/0968-0004(93)90040-t -
Antimicrobial Agents and Chemotherapy Mar 1992The activities of erythromycin and clindamycin against 350 Peptostreptococcus strains were studied during a 5-year period (1986 to 1991). In 5.1% of the... (Comparative Study)
Comparative Study
The activities of erythromycin and clindamycin against 350 Peptostreptococcus strains were studied during a 5-year period (1986 to 1991). In 5.1% of the Peptostreptococcus strains, which presented dissociated resistance (clindamycin MIC, less than or equal to 1 microgram/ml; erythromycin MIC, greater than 8 micrograms/ml), evidence of inducible macrolide-lincosamide resistance was shown. A total of 17.7% of the strains presented a constitutive phenotype; the clindamycin and erythromycin MICs for these strains were greater than 8 micrograms/ml.
Topics: Clindamycin; Drug Resistance, Microbial; Erythromycin; Microbial Sensitivity Tests; Peptostreptococcus; Phenotype
PubMed: 1622181
DOI: 10.1128/AAC.36.3.662 -
Journal of Clinical Microbiology Sep 1992Fifty-four strains of Peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot...
Fifty-four strains of Peptostreptococcus magnus (11 were recovered from abdominal infections, 18 were from nonpuerperal breast abscesses, and 21 were from diabetic foot infections; the type strain and three other strains were from the American Type Culture Collection, Rockville, Md.) and the type strain of Peptostreptococcus micros were tested for their ability to produce various enzymes, including catalase, hippurate hydrolase, serine dehydratase, threonine dehydratase, collagenase, gelatinase, alkaline phosphatase, and esterase C4. The data were analyzed by cluster analysis. The results showed that all but one strain could be assigned to either of two distinct, valid clusters. The first cluster of 11 strains was composed of strains that were relatively inactive, having produced one or two of the eight strain-dependent enzymes. The second was a large cluster of strains (n = 43) that were considerably more active, all having produced at least three enzymes; the vast majority of strains (89%) produced four or more enzymes. The unclustered strain produced one enzyme that was different from that produced by the strains in the first cluster. The chi 2 test of homogeneity applied to the clustering solution indicated that greater enzyme activity was significantly associated with the site of infection (P less than 0.001). The more enzymatically active P. magnus strains were recovered significantly more often from nonpuerperal breast abscesses and diabetic foot infections than they were from abdominal infections. These results may provide insight into the nature of certain polymicrobial soft tissue infections and suggest that (i) P. magnus may participate more in nonpuerperal breast and diabetic foot infections than in abdominal infections and that (ii) peptostreptococcal production of proteolytic enzymes may have an important adjunctive effect on the pathogenesis of certain soft tissue infections.
Topics: Abdomen; Abscess; Bacteria, Anaerobic; Breast Diseases; Diabetes Complications; Female; Foot Diseases; Gram-Positive Bacterial Infections; Humans; Peptostreptococcus
PubMed: 1400997
DOI: 10.1128/jcm.30.9.2330-2334.1992 -
Journal of Clinical Microbiology May 1994The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical... (Comparative Study)
Comparative Study
The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (Virginia Polytechnic Institute), the current "gold standard" method. A total of 5 Peptostreptococcus American Type Culture Collection strains and 95 clinical isolates comprising Peptostreptococcus anaerobius, P. asaccharolyticus, P. magnus, P. micros, and P. prevotii isolates were included for analysis. Overall, the sensitivity and specificity of the rapid ID32A kit in the identification for five Peptostreptococcus species compared with the Virginia Polytechnic Institute method were 93 and 80%, respectively. All P. anaerobius (n = 20) and P. asaccharolyticus (n = 25) isolates were identified with 100% sensitivity and 100% specificity. For the identification of P. magnus (n = 24) and P. micros (n = 19), the rapid ID32A kit was 100% sensitive for both species; the specificity for P. magnus was 95.8% and that for P. micros was 57.9%. The sensitivity and specificity of the rapid ID32A kit for identification of P. prevotii (n = 12) were poor (41.7 and 8.3%, respectively). The rapid ID32A kit is a useful method for the rapid differentiation of P. anaerobius and P. asaccharolyticus from other Peptostreptococcus spp. Conventional methods should be used to identify to the species level isolates of P. magnus, P. micros, and P. prevotii.
Topics: Bacteriological Techniques; Chromatography, Gas; Evaluation Studies as Topic; Female; Genital Diseases, Female; Genitalia, Female; Gram-Positive Bacterial Infections; Humans; Peptostreptococcus; Sensitivity and Specificity; Species Specificity
PubMed: 8051260
DOI: 10.1128/jcm.32.5.1302-1307.1994 -
Journal of Medical Microbiology Mar 2001Peptostreptococcus micros is a gram-positive anaerobic coccus which, although considered to be a natural commensal of the human oral cavity, is associated with...
Peptostreptococcus micros is a gram-positive anaerobic coccus which, although considered to be a natural commensal of the human oral cavity, is associated with periodontal, endodontal and peritonsillar infections. Identification of the organism has to date relied upon conventional culture methods and biochemical analyses. The purpose of this study was to develop a PCR method for rapid and specific identification of this organism in clinical samples. A pair of primers was selected, each of which was specific at the 3' end for P. micros DNA; they were used in the PCR assay, resulting in a 1074-bp product. The primers were shown to be specific for P. micros DNA as no PCR products were obtained when genomic DNA extracts from a wide range of other Peptostreptococcus species and other oral bacteria were used as templates. The PCR assay was then applied to the identification of P. micros DNA in subgingival plaque samples from adult periodontitis patients and pus samples from subjects with acute dento-alveolar abscesses. Confirmation of specific amplification of P. micros DNA was obtained by digestion of PCR products with the restriction endonuclease RsaI, which gives a unique restriction profile for P. micros, and DNA sequencing. Sixty-eight subgingival plaque samples from 18 patients were analysed, of which 19 (28%) were positive for P. micros DNA; the proportion of patients carrying P. micros DNA in at least one sampled site was 11 (61%) of 18. Twenty (71%) of 28 pus samples analysed by PCR contained P. micros DNA. These results confirm that P. micros may be involved in the aetiology of acute dentoalveolar abscesses and adult periodontitis. The PCR assay provides a more rapid and reliable alternative to conventional methods for identification of P. micros in clinical samples.
Topics: Adult; DNA, Bacterial; Humans; Middle Aged; Peptostreptococcus; Polymerase Chain Reaction; Sensitivity and Specificity
PubMed: 11232771
DOI: 10.1099/0022-1317-50-3-249 -
Clinical Gastroenterology and... Mar 2010
Topics: Aged; Animals; Bone and Bones; Female; Fishes; Foreign Bodies; Humans; Pancreatitis; Peptostreptococcus; Radiography, Abdominal; Tomography
PubMed: 19835985
DOI: 10.1016/j.cgh.2009.09.037 -
The British Journal of Dermatology Nov 2013The precise roles of Propionibacterium acnes and other anaerobic bacteria in the pathogenesis of acne are still unclear. Recent studies have shown that P. acnes can be...
BACKGROUND
The precise roles of Propionibacterium acnes and other anaerobic bacteria in the pathogenesis of acne are still unclear. Recent studies have shown that P. acnes can be further classified into several phylotypes with distinct phenotypes and virulence. Their distribution patterns in acne lesions have rarely been demonstrated.
OBJECTIVES
To analyse distribution patterns of P. acnes phylotypes and Peptostreptococcus species on the skin surface of patients with acne and healthy controls, and in comedones, papules and pustules from patients.
METHODS
A total of 370 samples from 95 patients with acne and 65 samples from 65 healthy controls were investigated. Three P. acnes phylotypes and three Peptostreptococcus species were identified by polymerase chain reaction primarily using type-specific primers.
RESULTS
There was no significant difference in the microflora of the skin surface samples between patients with acne and healthy controls. In acne lesions, distribution patterns between skin surface and comedonal lesions were similar, but they were significantly different from those of both papules and pustules. In the inflammatory acne lesions, the proportion of type IA P. acnes was increased, while those of type IB and II were decreased. The proportion of Peptostreptococcus species was also increased significantly in the inflammatory lesions.
CONCLUSIONS
At the precision of this qualitative study, our results do not provide any evidence that different phylotypes in the surface microflora might be important in triggering acne. However, type IA P. acnes and Peptostreptococcus species might be more closely associated with inflammatory acne lesions.
Topics: Acne Vulgaris; Case-Control Studies; Gram-Positive Bacterial Infections; Humans; Peptostreptococcus; Phylogeny; Polymerase Chain Reaction; Propionibacterium acnes; Skin; Skin Diseases, Bacterial
PubMed: 23795647
DOI: 10.1111/bjd.12486 -
Oral Microbiology and Immunology Feb 1998The prevalence of the smooth and rough colonial morphotypes of Peptostreptococcus micros was examined with culture technique in 123 patients with adult periodontitis...
The prevalence of the smooth and rough colonial morphotypes of Peptostreptococcus micros was examined with culture technique in 123 patients with adult periodontitis (age 24-68 years). Of all subgingival samples, 91% contained the smooth morphotype of P. micros. The smooth morphotype constituted a mean percentage of the total anaerobic viable biota of 6.0%, with a range of 0.02-35.7%. Of these samples, 49% contained colonies of the rough morphotype as well, with a mean percentage of the total anaerobic viable biota of 2.3% (range 0.01-16.2%). None of the samples contained only the rough morphotype. The total percentage of P. micros varied from 0.02-35.71% with a mean of 7.2%. No correlation was found between the prevalence of both morphotypes of P. micros and the age of the subjects or with loss of attachment or pocket depth.
Topics: Adult; Aged; Gingiva; Gram-Positive Bacterial Infections; Humans; Middle Aged; Peptostreptococcus; Periodontitis; Prevalence
PubMed: 9573826
DOI: 10.1111/j.1399-302x.1998.tb00754.x -
Clinical Infectious Diseases : An... May 1995
Topics: Endocarditis, Bacterial; Female; Gram-Positive Bacterial Infections; Heart Valve Prosthesis; Humans; Middle Aged; Peptostreptococcus
PubMed: 7620041
DOI: 10.1093/clinids/20.5.1431